台灣地形對颱風路徑的影響
颱風是台灣地區常見的天然災害之一，而台灣地區複雜的地形變化，也為颱風路徑的變化投下一個變數。根據以往的資料顯示，颱風路徑受到地形影響往往是在登陸前六小時內，而颱風對台灣地區災害最嚴重的時候往往是登陸前後三小時，也就是說要是颱風路徑受到地形影響發生偏轉，則我們只有數小時的時間來進行防災的準備。本研究主要收集1987~2001年由台灣東部登陸的台風最佳路徑圖，分別統計其颱風路徑或颱風環流過山時的變化，並分類統計各種不同颱風要素（包括颱風強度、行進速度、移入角、登陸地段等）對颱風路徑變化的影響，以做為未來預報之參考。結果顯示，台灣地形的確對颱風路徑有所影響，並使颱風在接近時偏轉，偏轉角度大多在30度以內，且偏北較偏南略多。而當颱風已偏東北方向接近時（移入角小於60度）向南偏轉較多；以偏東南方向接近時（移入角大於161度）方向偏較多，顯然地形對颱風行進有阻礙作用；而正交颱風偏轉角度也比斜交颱風小。在颱風強度部分，越強的颱風路徑偏轉角也越小，颱風過山後路徑受到導引氣流影響回到原路徑的機率較低。而影響颱風移動速度最主要的因素是導引氣流的強弱，若導引氣流受到山脈阻擋時，會使颱風移動速度減慢。而台灣各地山脈高低不同，也會使某些地區出現較特殊的台風現象。例如花蓮南部及成功地區容易誘發副低壓造成分裂路徑；台東地區因颱風容易繞山而過因此偏南機率比其他地區高等。Typhoon is a natural hazard that often occurs in Taiwan. The complex terrain of Taiwan also adds to the uncertainty to typhoon tracks. Based on past data, typhoons often show changes in their tracks within six hours before making landfall, while they produce most of their damage within three hours of the landfall. In other words, if the track of a particular typhoon was to be affected by the terrain, people have only a few hours to prepare. The study collects best tracks of a total of 145 typhoons that made landfall from the east of Taiwan from 1987 to 2001. Their tracks and changes in circulation while they moved across the Central Mountain Range are recorded, and classified according to several different elements such as strength, traveling speed, incipient angle, and landfall area. The possible influence of these elements on typhoon tracks can be applied to forecasts in the future. Results indicate that the terrain of Taiwan does have an impact on typhoon tracks, and causes typhoons to turn when they approach from the east. Turning angle in most cases is within 30°, and cases turning northward are slightly more than those turning southward. When typhoons approach Taiwan from the northeast (incipient angle 160°) they favor turning north. Also, the turning angle tends to be smaller for typhoons that approach the terrain at a right angle, and the opposite is favored when the incipient angle is more acute. This suggests that the terrain of Taiwan had a blocking and deflecting effect to typhoon tracks. For results related to typhoon strength, stronger typhoons tend to exhibit smaller turning in their tracks, but they are also less likely to return to their original direction of motion under the influence of steering flow after they leave the terrain. The most relevant factor to moving speed of typhoon is the strength of steering flow, which is slowed down if blocked by the terrain. The difference in terrain elevation can also influence the motion of typhoon and cause some rather special phenomena. For instance, a secondary low is more likely to form and result in a discontinuous track if a typhoon made landfall over southern Hwa-Lien or Cheng-kung, while a typhoon has a higher possibility to be deflected southward if it made landfall over Tai-tung, the southernmost area.
螯合劑對銅．鉛．鋅離子萃取．分離及測定之微型實驗研究
以微型實驗的方式用螯合劑：dithizone(diphenyl thiocarbazone)在四氯化碳中對金屬離子銅(Ⅱ)、鉛(Ⅱ)、鋅(Ⅱ)進行溶劑萃取與反萃取的平衡所得條件，以及由金屬離子與螯合劑結合時的莫耳數比與分離條件的探討得知，僅僅由控制溶液之pH值便可以使水溶液中的銅、鉛、鋅離子分離。於本研究中所使用之萃取光度分析法，對銅可以測至0-0.1ppm，鉛可以測至0-5.0ppm，鋅可以測至0-0.8ppm；莫耳吸光率分別為：Cu[HDz]2：4.50x104 L/moles‧cm(550nm)。Pb[HDz]2：6.85x104 L/moles‧cm(520nm)。Zn[HDz]2：9.50x104 L/moles‧cm(540nm)。其結果可做為重金屬離子廢水淨化效能之微型檢測指標。
The microscale experiment that the equilibrium of extraction and back extraction of Cu(Ⅱ)、Pb(Ⅱ)、and Zn(Ⅱ) with the extraction of chelate agent- diphenyl thiocarbazone(dithizone) in carbon tetrachloride were investigated. The conditions of extraction and back extraction of pH1/2 and the stability of these chelate compounds have the possibility to separate these Cu(Ⅱ)、Pb(Ⅱ)、and Zn(Ⅱ) mental ions in aqueous solution or in water sample mutually.
The separation and determination of Cu(Ⅱ)、Pb(Ⅱ)、and Zn(Ⅱ) up to 0~1ppm(Cu), 0~5ppm(Pb), 0~0.8ppm(Zn) with dithizone in CCl4 by extraction spectrophotometry has been established.
The molar absorptivity expresssed in Lmol-1cm-1 was found to be 4.50x104(550nm)for Cu[HDz]2, 6.85x104(520nm) for Pb[HDz]2, 9.50x104(540nm) for Zn[HDz]2, respectively.
銅影響大豆及田菁根部生長、細胞死亡和訊息傳遞
隨著迅速的工業化，重金屬汙染已是嚴重的環境問題。在植物中，當植物體內累積過量的重金屬，對於植物根部、葉部等器官的生長與發育有嚴重的影響或傷害。銅離子為植物生長所必須之重金屬，但是過量銅離子會導致細胞死亡，生長受到抑制。本實驗以大豆( Glycine max )及田菁( Sesbania roxburghii )為植物材料，藉由Evans blue 染色法、螢光染色、西方墨點法、反轉錄聚合?鏈鎖反應等，觀察過量銅離子影響植物根部生長、細胞死亡和細胞訊息傳遞物質變化之情形，並探討過量銅離子影響大豆根部細胞死亡的訊息傳遞路徑。過量銅離子會限制植物根部的生長及造成根部細胞死亡。以螢光染色觀察根尖(ROS, reactive oxygen species)、Ca2+累積情形，根尖細胞Ca2+、ROS 累積隨處理銅濃度的增加而上升，可能影響細胞死亡程度。以Ca2+螯合劑EGTA 和W-7(CDPK(calcium-dependent protein kinase)、Calmodulin 抑制劑)前處理發現可以降低過量銅離子對大豆根部的細胞死亡程度，推測Ca2+、CDPK 參與銅引發大豆根部細胞死亡的途徑。為檢驗MAPK 參與根部細胞死亡的途徑，以西方墨點法偵測根部細胞MAPK 的TEY 或TDY 磷酸化，實驗結果發現，隨著過量銅離子濃度的升高，田菁、大豆根部42-kDa MAPK磷酸化情形有上升之趨勢。以RT-PCR 分析大豆MAPK1 及MAPK2 基因之表現量，發現在銅處理時大豆之MAPK1 和MAPK2 基因的轉錄情形增加。大豆( Glycine max )及田菁( Sesbania roxburghii )皆屬於豆科植物，可作為綠肥植物。探討銅影響大豆、田菁細胞死亡之訊息傳遞路徑，希望進而控制生物體所受的毒害情形及訊息傳遞途徑，加強生物體對重金屬的防禦機制，未來可以以基因轉殖等基因工程技術，轉入抗重金屬基因或增強植物體對抗重金屬的能力等，作為綠肥植物、抗重金屬植物吸 附重金屬來復育土地達綠色淨化等用途。 Many heavy metals are necessary for plants, but excessive quantities directly affect plant growth and survival of organisms, cause cell death, or even affect human life indirectly. Cu (copper ion) is a heavy metal, which is one of micronutrients essential for normal growth and development of plants. The purpose of this experiment is to study the effect of excessive copper on Glycine max and Sesbania roxburghii root tips. I conducted some experiments by means of Evans blue staining (analysis of cell death), western blot analyses, and fluorescence microscope in order to examine the way copper results in plant death. Measurement of root length and analysis of cell death showed that excessive copper could bring about the inhibition of plant growth as well as cell death. With fluorescence microscope, I found that excessive copper might increase the level of copper-caused reactive oxygen species (ROS) in both the root tips of Glycine max and Sesbania roxburghii. In addition, I used Oregon Green 488 BAPTA-1 so as to assess the accumulation of calcium ions in root tips and found that the exposure of root tips to excessive copper results in the accumulation of calcium ions. To investigate whether calcium ions and calcium-dependent protein kinase (CDPK) play a role in the cell death caused by excessive copper, I tested W-7, calmodulin and CDPK inhibitors, and EGTA, Ca2+ chelating agents, before copper treatment – immersing copper in CuCl2. In this way, plant cells would be effectively prevented from copper-caused death. Besides, to find out whether copper activates MAPKs in plant cells, I took advantage of western blot analysis with Phosphor-Map kinase Antibody and Map kinase Antibody. The results revealed that excessive copper might lead to TEY or TDY motif phosphorylation of approximate 42- and 64-kDa MAPKs in the cells of Glycine max root-tip and approximate 42-kDa MAPKs in the cells of Sesbania roxburghii root-tip. Furthermore, with RT-PCR, I found that the transcription of Glycine max MAPK1 and MAPK2 mRNA happens more frequently in root cells after copper treatment. In addition, this study suggested that the MAPK cascade CDPK pathway may function in the heavy-metal-signaling pathway in plant, and that calcium ions and ROS might get involved in the copper-caused death of plant cells. By studying signal transduction against heavy-metal toxicity in the plants, we can know how the organisms protect themselves. Sesbania roxburghiivv (or Glycine max), as green manure, could be used for metal-hyper-accumulator with the help of genetic engineering in the future.
仲夏夜裡的精靈－探討發光胺之化學發光反應與催化劑之作用
過去，螢光的使用只局限於釣魚、登山等無法使用電燈或火把時使用的一種較為安全的冷光。而今，我們使用這種化學發光的機會也越來越多，也再成了更多的汙染，所以我們想藉此去研討有關螢光棒之化學反應與其反應之改良。在這一篇報告當中，我們討論與研究有關Luminol發光之反應與催化劑對其反應之影響；比較在380nm~480nm範圍內不同波長所產生之光度及比較各種不同催化劑在相同波長的發光度隨反應時間的變化。我們發現在此反應之中，以k3Fe(CN)6可以產生出最大的亮度，且由實驗的結果得知Luminol的發光無法維持兩分鐘，發光時間較為短暫。映之催化效果是同時被金屬離子和根離子影響。具有明顯催化效果(最大光度超過2.5)的鹽類濃度以稀薄為佳，約10-3M。在此反應中以k3Fe(CN)6為其催化劑，可以產生一種穩定且明亮的發光，是一種較佳的催化劑在此化學發光反應之中。In the past, fluorescence was limited in being used in fishing or hiking, in which light or a torch was not available. Fluorescence is much safer because of its feature of luminescence. Today, the opportunities we use this fluorescence become more and more. The more people will use fluorescence. The more environment pollution will be caused, that is the reason we would like to study the chemical reaction of fluorescence and its solution to reduce pollution. In this paper, chemical reactions between the Luminol and different catalysis agents are studied, the comparisons between the reaction condition of the catalysis agents and the Luminol, to measure the light intensity variation in 350-500nm light wavelength range. And to measure the light intensity variation following the time of the chemical reactions between the Luminol and different catalysis. We found out that the chemical reactions between the Luminol and k3Fe(CN)6 being the catalysis agents can produce the maximum light strength. But the time of the chemical reactions is much shorter, it only can keep this chemical reactions operating in two minutes. The chemical reaction’s catalysis agent is affected by metallic ion and SO4(2-) , NO3(1-),Cl(1-), when the catalysis agent’s concentration is sparely, this luminous reaction is more obvious( the maximum light strength is over 2.5) .It can produce a fluorescence which is steady and luminous, and it is better to become the catalysis agent material of the fluorescent chemical reactions.
Vitamin E 對回鍋油品質的影響
家庭中為了節省油量的使用，常將所剩的油品另存於容器中，以待下次使用。近年來，已有不少文獻報告指出，這種「回鍋」的使用方式，往往使得油品品質變得相當低劣。近來市面上有不少的油品，標榜添加天然維生素E，使消費者趨之若鶩，作為一種吸引消費的手段；這激發了我們極高的興趣，因為在文獻中已知道維生素E在室溫下確實可抑制油品的氧化；但添加的油品經過回鍋後，其對油品的影響卻仍不得而知。我們利用鐵鍋油炸澱粉食物，並經由反覆的油炸與冷卻，模擬更真實的回鍋情形。並添加抗氧化劑，包括維生素E和酯化的維生素E，以及回鍋次數等不同的變因進行實驗，進行油品品質的化學變化的測量。經由實驗結果顯示，經數次的回鍋烹煮，油品品質因為一連串的化學反應變得十分低劣；另外，添加酯化的維生素E的油品在某些方面的確減少了某些不良生成物的形成(如游離脂肪酸、羰基化合物等)，但在某些方面如氧化程度的比較(共軛雙鍵的生成)卻與一般無添加的回鍋油無異。反倒是添加維生素E對於回鍋油品質的影響在實驗結果的每方面看來，不管是酸敗氧化或是裂解成羰基化合物都能夠有效地減少或產生抑制的作用；故添加維生素E對於回鍋油的品質的確有正面性的影響！Cooking oil is often reused in homes in order to economize on its use. In the past few years, many reports have pointed out that ”reusing” oil this way always changes the properties of oil and makes it spoiled. Also in the past few years, many oil products have appeared on market that highlight the fact that vitamin E has been added, in order to attract consumers. This attracted our interest, since we know that on the basis of several studies, vitamin E indeed has an antioxidizing effect at room temperature or below. However, it is not know what effect vitamin E has on re-cooked oil. Therefore, in this experiment we simulated the re-cooking process by frying steamed dumplings in an iron wok with soybean oil, and repeatedly cooling and re-using the oil. Variable factors were the added antioxidants(vitamin E and vitamin E acetate) and the number of re-cooking times. The chemical variation in the resulting oil was tested with regard to acid value, viscosity, carbonyl value, and the value of conjugated bonds. The results indicated that as the number of re-cooking times increased, the quality of the oil had undergone a series of chemical reactions and became spoiled. Also, the addition of vitamin E and vitamin E acetate indeed reduced the formation of certain unhealthful products such as free fatty acids, carbonyl groups, and so on. In certain respects, such as the quantity of oxidized components in the oil, there is no obvious difference between ordinary oil and oil with the vitamin E acetate additives. However, the addition of vitamin E to cooking oil, based on the results of this experiment, can effectively reduce or suppress processes causing rancidity and breakdown into carbonyl groups. Therefore, the addition of vitamin E or vitamin E acetate dose have a positive effect on re-cooked oil.
八分鐘快速免疫呈色法檢測市售牛乳中有無摻雜粉
台灣過去40 年來，許多牛乳廠商會因牛乳供應量不足摻雜奶粉以增加利潤，為了解決這問題，本實驗提供一種快速且準確的免疫呈色法檢測牛乳中有無摻雜奶粉。因奶粉的加工過程中加熱是必須的，所以本實驗是利用單株抗體只與牛乳中因加熱而變性的蛋白質反應。實驗步驟非常簡單，首先，用一支玻棒沾附待測鮮乳，經過簡短的清洗及化學處理後，玻棒尖端會與經特殊製備的單株抗體反應。最後，將玻棒放入已製備好的溶劑中呈色。當溶液呈現綠色，表示待測乳中摻有奶粉。整個實驗流程只需短短八分鐘。據我所知，該方法是相當具有新穎性且從來未被應用過。這是個令人興奮的發現，足以解決多年來酪農業中牛乳摻雜奶粉的問題。For the last 40-years, owing to the limited supply of commercial raw milk, the industry sometimes would mix the dry milk into the raw milk to increase their profit. To detect whether or not the milk on the market contains the poor quality’s dry milk, I invented a rapid and sensitive colorimetric immunoassay. The assay essentially utilizes a monoclonal antibody that only reacted with the thermal denatured protein presented in the dry milk. First, a glass tip-stick was dipped in to the milk to be tested. Second, following a brief wash and chemical treatment, the glass tip was reacted with the monoclonal antibody that has been specifically produced. Finally, the glass tip was dipped into a reagent containing developer. When the solution develops a color in green, it indicates the milk definitely contains dry milk. The entire procedure only takes 8 minutes to finish. To the best of my knowledge, this method is novel and has never been shown before. It represents an exciting discovery that solves the malpractice of mixing dry and raw milk in our dairy industry.