捷克

My thesis focuses on studying Cretaceous microfossil specimens from the excavation of former brickworks in Štíty, especially foraminifera. In the theoretical part, I have covered the structure of the Bohemian Cretaceous Basin area, especially Bystřice Lithofacial Development. I have also processed previous paleontological researches from the locality. Emphasis was placed on field research and subsequently on laboratory research of the site. I have examined the present state of the location and gathered samples of silt clay containing a wide variety of fossils. I have acquired the microfossils, determined them, and ordered them systematically. The most important part of the thesis is the systematic and palaeoecological processing of the collection of microfossils from the locality. The thesis continues the research of the last year of SOČ, where I have gathered a collection of fossil macrofauna, flora, and ichnofauna. My collection is supplemented mainly by benthic and planktonic foraminifers. I have confirmed that the specimens found are typical representatives of marine fauna belonging to the Upper Cretaceous Coniacian. The paleoecological characteristics of the locality correspond to a nutrient-rich shallow-water environment, occasionally disturbed by storm waves.

The occurrence and behavior of insects is significantly affected by the environment they live in. In this thesis, I dealt with the influence of structure of landscape on nesting preferences of Hymenoptera. For this comparison I had chosen to work with twig-nesting Hymenoptera, for which I have placed artificial nest opportunities into four biotopes – heath, edge of a heath, country lanes between fields and field. The studied location is located south of Znojmo near Podyjí national park. Particularly, I have focused on small carpenter bees of genus Ceratina. My results show that there is a big difference in the species distribution between the habitates of field and heath. The habitats of edge of a heath and country lanes make up a gradient between these two biotopes. The ecosystem of fields was preferred by a sphecid wasp Pemphredon lethifer and Ceratina cyanea. On the other hand, the ecosystem of heath was preferred by Ceratina chalybea, Ceratina nigrolabiata and megachille bee Hoplitis tridentata. I had also found out that in species that are more abundant on heaths, there is higher mortality of offsprings. Heaths were a place of the highest competition between species, as a result of which species with a lower body weight (Ceratina nigrolabiata) have been pushed into narrower twigs by larger species (Ceratina chalybea). I recorded a high number of nests in biparental bee Ceratina nigrolabiata, which were guarded only by mother in fields and country lane habitats. This may indicate that Ceratina nigrolabiata is only facultatively biparental, not strictly biparental as was considered until now.

Silica capillaries have been an integral part of the instrumentation used in many areas of analytical chemistry for decades, especially in analytical separations. In most cases, they are used without treatment, occasionally forceless chemical surface treatments are made to suppress or enhance the activity of silanol groups. The aim of this work was to disrupt the inner surface of the capillary, perfectly smooth from manufactory, so that relatively coarse and various structures would be created, and to study their influence on the separation efficiency. The uniqueness of the used solution is based on the use of special properties of water exposed to high temperatures and pressures (supercritical water), which is able to disrupt this chemically inert material because of its aggressivity. In total, over 2000 experiments were carried out in order to define conditions suitable for the formation of various types of surface structures. Due to the high amount of resulting data, our own database application was created, allowing not only to save the picture of the structure and experimental conditions information, but also to clearly sort them out and create image reports according to the specified parameters. Samples representing individual types of structures were then selected from this database and a number of silica capillaries with a configuration suitable for electromigration analyzes were prepared. The creation of a structured surface in the input part of the separation capillary enabled the separation of some classes of substances and biosamples, which cannot be analyzed on standard capillaries with a smooth surface. An example is the complete separation of two strains of Staphyllococcus aureus bacteria (MRSA and MSSA), or the use of the absorbing capabilities of a structured surface to study the interactions of these bacteria with bacteriophages. This ability was also used in the determination of Aspergillus fungus in a sample taken directly from the patient's lungs, where there was achieved a significant increase in the sensitivity of the analysis. Structured capillaries can also be used in the analysis of food samples, i.e., for the separation of β-lactoglobulins A and B in cow's milk, which belong to its main allergens.

In the present paper I studied the winter territories of the Eurasian Wren (Troglodytes troglodytes) in the urban environment of the city of Hradec Králové. The males of this species were detected through the aggressive reactions to the playback of its conspecific call. The Eurasian Wren territories were detected at seven out of the ten observation points in the city. The frequency of the territories decreased during the observation period from October to January and it was also affected by the structure of the biotope. It seemed that the wrens preferred trees and avoided lawns and isolated growths. That illustrates how important it is to maintain wildlife corridors in the cities. Individuals of seven other bird species were seen reacting to the playback of Eurasian Wren songs or warning calls. Eurasian Wren may be applied as an indicator species of the ecological value of the urban environment. Through this species, we can access such quality also for the human inhabitants.