全國中小學科展

2015年

『凍』人心『鹹』,『黃』金Style~以冷凍凝膠法創作速成鹹蛋黃之新『蛋』生

蛋黃酥所使用鹹蛋黃來自於鴨蛋鹽漬而成,取出鹹蛋黃剩餘之蛋白因鹽度高,無法再利用只能拋棄相當不環保,本實驗利用將蛋冷凍再解凍以分離出蛋白及蛋黃,蛋白可加工再利用,凝膠蛋黃則可鹽漬成鹹蛋黃。結果發現蛋黃在-18℃冷凍3天可完全凝膠,以30%食鹽水鹽漬40-60分鐘,其鹽度已與市售鹹蛋黃相當。在喜好性感官品評發現,鹹味、口感及整體喜好性均與傳統鹹蛋黃相似。解凍蛋白製成蛋糕在喜好性感官品評發現,色澤及口感評分最高優於新鮮蛋白,在香味及整體喜好性則與新鮮蛋白無差異。速成鹹蛋黃僅須1/10生產時間且蛋白可回收再利用並減少鹹蛋白廢棄物。

轉角遇到愛-街道方格中不期而遇的機率

在m×n的街道方格上,甲從左下到右上,乙從右上到左下,沿格線走捷徑前進,探討兩人相遇機率。 依相遇點型態不同,相遇機率分為m+n為偶數與奇數兩種形式。當分岔口選擇路徑之機率均等時,發現偶數時的機率大於奇數的機率,並證明若固定n值,則相遇機率隨m增加有鋸齒狀的下降趨勢。另外,在m+n為大於5的定值時,街道方格越接近正方形,相遇機率越大。 當選擇路徑之機率不均等時,m+n為偶數時的相遇機率不必然大於奇數的機率,並且當選擇路徑之機率的比值接近m/n時,可得最大的相遇機率。同時分析了相遇機率圖形的上升、下降趨勢與鋸齒狀等多種樣貌的形成條件。

探討正n邊形的內接正三角形

對於一個正n邊形,若一正三角形三個頂點分別位於正n邊形的三個不同邊上,則稱此正三角形為該正n邊形的內接正三角形。本次科展的主題,就是探討正n邊形的內接正三角形之作圖方法,以及作出的圖形中各種漂亮性質。

狐亂發臭-腋下細菌叢林的秘密

根據西方文獻造成狐臭的原因之一是因為Staphylococcus 將汗液中的白胺酸(leucine)代謝產生異戊酸(isovaleric),散發出臭味,但台灣人的狐臭菌是否與西方人類似則較少被探討。 本研究透過好氧和厭氧的篩選方式篩出的優勢腋下菌種以Staphylococcus epidermidis、Staphylococcus haemolyticus、Staphylococcus lugdunensis、Micrococcus luteus、Staphylococcus aureu 為主,與國外研究結果略有不同。在Gas Chromatography (GC,氣相層析儀) 檢測結果發現以上菌種中能將白胺酸轉換成異戊酸,效果最好的菌是Staphylococcus lugdunensis,推測Staphylococcus lugdunensis為造成狐臭主因。 由結果推測,異戊酸只是臭味的一部分,未來可以藉由分析汗液成分與腋下菌的產物來找出導致狐臭的其他原因,找出更完整的腋下作用機制及更好改善狐臭的方法。

「心心」照我「心」-從Pascal's theorem、Brianchon's theorem到雙心多邊形的共點共線性質探討

本研究靈感來自對Brianchon定理「圓外切六邊形的相對頂點的三條對角線共點」及Pascal定理「圓內接六邊形的三組對邊延長線交點共線」這兩個對偶定理的性質探討,進一步研究其在雙心六邊形共點共線的可能情形。研究結果有許多驚人的發現,特別是其三條對角線以及三條對邊切點連線,有六線共點,此點為定點,且與其外接圓圓心、內切圓圓心三點共線,且此線與Pascal Line垂直。據此,更進一步對雙心六邊形退化與延伸情形作深入探討。

Ancient Medicine- Modern Approach

The apricot kernel is believed to have a great medicinal value in many cultures. However, literature and research indicates that this belief still remains extremely controversial and conclusions regarding the medicinal value are ambiguous due to the presence of cyanide in the kernel. The focus of this research was to evaluate two objectives through the use of several integrated technologies and modified methods: (a) To successfully remove the cyanide from the apricot kernel using an adapted method; (b) To determine the effects of the cyanide free apricot kernel extracts on Helicobacter pylori and Streptococcus pyogenes bacteria. Procedures The apricot kernels were removed from the pits and then ground using a food processor. The kernels were then tested for cyanide using a cyanide test kit and Cyantesmo test tape. These tests indicated that cyanide was present. A novel approach was devised to remove the cyanide and when retested, the kernels tested negative for cyanide. This result was confirmed with Infrared Spectroscopy. The cyanide free kernels were then extracted using a Soxhlet Extractor with methanol for 24 hours. In addition to the methanol extraction, three other techniques were used to obtain kernel extracts: (a) Celite filtration, (b) Infusion Method A, (c) Infusion Method B. The Kirby Bauer method was modified for the microbiology aspect of this project. The Helicobacter pylori and Streptococcus pyogenes bacteria were plated using a 0.5 McFarland Standard. Paper filter discs containing 20µL of each extract were placed onto the inoculated plates in replicates of nine. After 48 hours of incubation, the zones of inhibition were read for each plate. Data The results were extremely encouraging and therefore to ensure the accuracy and preciseness of the data collected, four statistical analyses were completed. These include Confidence Intervals (CI), Standard Deviation (STDEV), T-Tests, and Chi Tests. The methanol extract was significantly different from the control in all trials. The Chi test also yielded a Chi Square value of 223, which was significantly greater than the critical value of 15.507, indicating that the results observed were not due to coincidence. Conclusion Literature evidence has indicated that the apricot kernel has been and is still used for medicinal purposes. Studies have shown that the presence of cyanide and the risks associated with this compound outweigh any benefits gained from the kernel. It has also been suggested that previous bacterial testing resulting in positive inhibition may have been due to the presence of cyanide. However, in this study, I was able to remove the cyanide through an innovative method to prove that the biological activity observed was highly unlikely to be due to cyanide. This indicates that there are other compound(s) in the apricot kernel that have specific antibacterial properties. The potential to improve the quality of life through the application of the apricot kernel appears to be supported, and further studies justified at this time.

間質幹細胞的條件培養基在增進胚胎幹細胞生長、移動及移植成功率所扮演的角色

胚胎幹細胞是一種高度未分化細胞,因具有分化成許多組織構造的能力,所以可用來治療疾病。但在移植胚胎幹細胞至人體時,經常無法順利增生和移動到目標組織。而先前的研究發現間質幹細胞條件培養基可促進皮膚細胞移動,所以我們假設間質幹細胞條件培養液能增進胚胎幹細胞的移動與增生,並且有潛力應用於臨床上。 本研究中,我們發現間質幹細胞條件培養基能促進胚胎幹細胞的移動,而在細胞增生方面僅能維持基本生存。未來我們將進行動物實驗,以了解間質幹細胞條件培養基對於細胞分裂與移動是否有幫助及是否具臨床上的應用性。

COMPARATIVE STUDY OF THE ELECTRICITY GENERATED FROM FRUIT EXTRACTS OF CALAMANSI (Citrofortunella microcarpa), CAMIAS (Averrhoa bilimbi), AND STARFRUIT (Averrhoa carambola)

The study aimed to compare the electricity generated from the fruit extracts of calamansi, camias and starfruit. Unripe fruits were extracted and varied percentage compositions of each extract were prepared.Wires wereconnected to a multi-tester (voltmeter or ammeter) to measure voltage or current that passes through. Results revealed, that amount of voltage and electric current generated are its lowest reading at 25% and are its highest reading at 100%. Nonetheless, of the three fruit extracts, it’s the calamansi that has the highest amount of voltage generated of0.97 volt while camias has the highest amount of electric current generated of 13.98 mA. Using ANOVA at 0.05 level of significance on the amount of voltage generated among varied percentage compositions of three extracts. However, there’s a significant difference on the amount of electric generated among varied percentage compositions. Results of ANOVA statistically signify that the three different extracts could either be used as a source of voltage and that camias extract should be preferably used over the other two fruit extracts in generating electric current. In all compositions, produced voltage is between 0.88 and 0.97 volts and current is between 3.28 and 13.98 mA. These currents produced are not enough to turn on a small light bulb having a smallest voltage capacity of 1.2 volt, but can be able to turn on a light-emitting diode (LED) that require such amount of current.

"癌"究-小花蔓澤蘭

外來入侵種小花蔓澤蘭繁殖速度太快,使台灣本土生態系受到嚴重破壞。我們研究發現,小花蔓澤蘭葉和根莖具有保護自由基誘發劑AAPH誘導紅血球溶血及清除DPPH自由基的能力。小花蔓澤蘭葉和根莖總多酚含量可達51.6及20.9 mg/g,推測其所含多酚化合物可能是抗氧化活性的來源。抗癌研究發現,小花蔓澤蘭葉和根莖會誘導人類急性骨髓血癌(HL-60)細胞毒性(24小時IC50為129及203 μg/mL)。顯微鏡觀察下,血癌細胞有減少和皺縮現象; TUNEL試驗法發現,血癌細胞DNA斷裂; 西方墨點法發現,Caspase-3蛋白增加、Bcl-2蛋白減少、Bax蛋白增加及PARP蛋白裂解; 推論小花蔓澤蘭可能誘發血癌細胞凋亡。裸鼠移植腫瘤模式證實,小花蔓澤蘭確實具有抑制活體血癌腫瘤及誘導血癌細胞凋亡之功效。總結,小花蔓澤蘭具抗氧化及抗血癌功效,可開發成為預防自由基疾病及抗癌的保健食品。

以分頻多工及交流差動磁場強度為基礎之定位系統應用於無人飛行器控制系統的研究

本研究提出一套創新的定位系統,僅需設置單一已知參考點即能達成高精確度的定位,由該參考點上的兩個永久磁鐵的靜磁場轉換成時變場型之交流磁場,再透過分頻多工的數位訊號處理,分離出這兩個磁場源之差動磁場強度及其相位,以求得物體的位置資訊,達成不受空間中的多重路徑干擾及障礙物影響的精確定位。透過實驗證明,本套系統在實驗設定下具有10公分以內的定位精準度,同時可以支援微型無人飛行器的即時定位,對於未來各種行動裝置、穿戴科技與自動控制系統之定位需求,可提供具體的貢獻。