自由基VS抗氧化物
自由基會產生在神經系統、免疫系統、血液循環系統等等，進而影響到人體各器官的運作,甚至於近年來許多醫生學者提出自由基病理:自由基是百病之源。本次實驗筆者挑選葡萄子、維生素C、綠茶來抑制清氧自由基(OH.)所採用的方法是將10%雙氧水製入注射筒並加亞鐵離子催化,,使其與抗氧化物反應,由於雙氧水分解會產生氫氣自由基與氧氣,因此筆者用倍率放大器(OPA)放大生成氧氣造成的電壓,並用Data Studio測量記錄,最後可由氧氣體積對電壓的趨勢圖看出抑制氫氣自由基的效果;Free radicals will be produced in our nerves system blood circulation immunization system etc. and they able to influene the operaion for our organs many medical scholars have even come up with "free radical pathology"-free radicals are sourse of all he diseases in recent years.In this study, I chose rape stone vitaminC and green tea to restrain hydroxide radicals(OH.) Here is summary of the experimental process. First,I put 10%hydrogen peroxide into an injector and then added ferrous ion to hydrogen peroxide to catalyze it. Second I let it reaact with the sample. Because hydrogen peroxide can produce hydroxide radicals and oxygen, I used the mutiplier(OPA) to amplify the pressure caused with the prducion of oxygen, measuring and recording resuls by the software"Data Studio"Finally, we can tell which antioxidant is more effective in restraining hydrode radicals from volume-voltage gragh.
電容超音波膠體金粒子電位調控系統研發
費曼曾說：There is plenty of room at the bottom。喬治亞理工大學的Mostafa El-Sayed 教授發表的癌細胞辨識、與科學月刊報導『台大抗煞一號』引發我們對膠體金粒子的興趣。膠體的性質主要是由界達電位 (zeta potential)決定。參考台科大、成大、中山…等超音波應用研究，提出改良篩選物理法製造之膠體金粒子的儀器設計與製作。經沉降過濾可達平均粒徑 100 nm；而離心式篩選機與超音波管式篩選機可達平均粒徑30 nm。篩選後的膠體粒子以電容原理調控膠體金粒子之界達電位 (zeta potential)，成功地從-30 mV 提升至-59 mV，並發展成電容超音波界達電位控制儀(Capacitor Ultrasonic Zeta Potential Controller)。以膠體金粒子與蛋白質鍵結量來測試調控界達電位的效果，發現蛋白質鍵結量之增加曲線與界達電位的增加曲線的增加趨勢相似；此功能的發現對於生物科技方面的應用應會有很大的幫助。透過界達電位控制系統，本研究達到費曼先生所期望的「在原子或分子的尺度上來加工材料和製造設備」。“There is plenty room at the bottom.” The words of Mr. Feynman are the beginning of nano technology. Mostafa El-Sayed, a professor of Georgia Institute Technology, identified cancer cells through nano gold-antibody complex. So, our study focuses on the zeta potential of colloidal gold particles. At first, the filtering method and equipments were developed. The theories were based on the ultrasonic studies of universities such as National Taiwan University of Science and Technology. Then the colloidal gold’s sizes were filtered to100 nm through settling. At last, by using Continual-Filtering Centrifuge (CoCe.) and Tube Well Mass (TW-MS), the mean particles sizes can be filtered to 30 nm. The most important results are: Zeta potential of the gold colloid was controlled with Capacitor Ultrasonic Zeta Potential Controller. The zeta potential can be raised from -30 mV up to -59 mV, which is -20 mV higher than the conventional pH-changing way. The function of zeta potential to protein binding quantity was tested. The increasing curves of zeta potential and protein binding quantity were similar. This property would be a significance of biotechnology. Thourgh Capacitor Ultrasonic Zeta Potential Control system, the zeta potential’s limitation of gold colliod, which is produced by SANSS (Submerged Arc Nanoparticles Synthesis System), can be controled in a wilder range. The study which is focused on nano-scale, like the wish of Mr. Feynman – “To manufacture material and produce equipment in atom and molecular scale”.
明察秋毫-金屬的熱膨脹
Thermal expansion exists in our daily life. However, thermal expansion is generally too slight to be seen by naked eyes. Therefore, in the present project, a dilatometer was assembled to enhance better sensitivity toward thermal expansion. Hopefully the self-assembled dilatometer could contribute to teaching purpose.The structure of our 4th generation dilatometer is showed below. Using an ‘L’ square to hang up the metal stick and a rolling needle with a mirror to reflect the laser light are the critical parts of this equipment. By using this special reflection mechanism, the slight expansion of a metal stick caused by heat can be enlarged to a large scale. This special mechanism is where our creativity laid. Measuring in millimeter (mm), the measurement precision of the equipment can be extended to 0.0001 decimal. Our dilatometer was used to measure the expansion of various metal sticks caused by the temperature changes. Results were drawn from analysis of the data: 1) The average relative deflection was within 1.0~1.8%; 2) The relative deviation of linear thermal expansion coefficient was within –1.2~-4.4%.
物質熱漲冷縮的特性普遍存在於我們的生活環境中，但因其變化量相對微小，一般並不容易直接觀察，爲了進一步研究這課題，我們組裝偵測熱膨脹的儀器，並希望儀器的靈敏度高，能推廣為教學器材，經過我們不斷努力與改良，終於有了令人愉悅的成果。
自製第四代熱膨脹儀的結構如圖，設計「角尺懸吊金屬棒」與「滾針及鏡面反射」是儀器的重要部份，利用滾針旋轉及鏡面反射雷射光，加乘放大熱膨脹的微量變化，這是我們主要的創意，以公厘（mm）為單位，儀器的精確值到小數第四位。
利用自製的熱膨脹儀，探討金屬熱膨脹的影響因素。分析實驗所得數據，平均相對偏差在1.0~1.8﹪，而線膨脹係數的相對誤差約-1.2~-4.4﹪。
奈米溶膠製成蓮花效應及應用
Lotus effect（蓮花效應）是蓮葉表面化學組成（wax）與物理組成（微纖維結構）兩者所造成。本研究是以模擬Lotus effect，採用Sol-Gel 製成，將氟化矽聚合為奈米膠體。實驗結果發現，以異丙醇為溶劑，再依序加入氟化矽、硝酸以製成的Sol-Gel，將其塗覆於玻璃表面，可得到最高的接觸角（114.71°），且少量的氟化矽可製成大量的成品，已具有實用價值又兼顧成本的優點，最重要的是，本研究克服了目前Sol-Gel 製程與應用的四大難題（機械強度、與基材接著問題、透明度、溶膠凝固問題），可說是一大創舉。利用所研發出來的奈米溶膠，我們能成功地將Sol-Gel 附著於布料、玻璃、釉表面、粉體，也能成功地研發出具有自潔透氣的布料、救生衣、雪衣、棉被及自潔功能的玻璃、磁磚與市面上尚未研發出的防水粉體（接觸角＞140°），因此我們研發出的Sol-Gel 應用甚廣，有無限的發展潛力。Chemical composition (wax) and physical characteristics (microstructure) of lotus leaves are both responsible of the so call Lotus Effect. In this study we intend to demonstrate louts effect by applying Sol-Gel method to polymerize fluorosilane into nano-scale colloid. Our experimental results shown that the sol-gel made based on isopropanol solvent with fluorosilane and nitric acid added in order, when coated on glass plate, can achieve highest (liquid-surface) contact angle of 114.7 degrees. In addition, only small quantity of fluorosilane is sufficient to produce large amount of product, making this method feasible and cost-effective. More importantly, this procedure overcome the four major difficulty of sol-gel processing and application, namely mechanical toughness, adhesion with substrate, transparency, and consolidation. Using the nano-sol-gel developed in this study, we have successfully coated the sol-gel onto fabric, glass, ceramic grazing surface, and powder, which allow one to make self-cleaning breathable clothes, life jacket, snow cloth, futon and self-cleaning glass and tiles, as well as water-proof powder (contact angle > 140 degrees) which is brand new on market. We therefore believe that there is a great potential for the application of sol-gel developed in this study.