耐熱性酵素生產菌Bacillus Subtilis WLA12 之分離與定性
本實驗針對一取自台北縣烏來南勢溪下游之溫泉菌，進行微生物學、生物化學、分子生物學三方面之觀察與實驗，期能得到一可生產特定酵素之菌種，且具耐高溫之抗逆境能力。對其酵素進行定性，並嘗試轉殖出相關基因，使之可大量表現。目前已篩選出一種可生產多種酵素之菌種〔依其採集地點暫稱WL-A12〕經菌種鑑定為Bacillus subtilis WL-A12。藉由菌落檢測法以及Zymogram 的方式做酵素分析，並以電導轉形等技術，希望能成功轉至E. coli 上表現。另外，也對該菌種作了一些基本微生物方面的觀察〔如需氧情況、最適生長溫度〕。We isolated enzyme-producing thermophilic bacteria from hot springs near downriver of Nan-shi, Wulai, Taipei (北縣烏來南勢溪). Through microbiological, biochemistry and molecular biological analyses, a multiple enzyme-producing Bacillus subtilis strain, designated WLA12, has been isolated. The growth condition of WLA12 was observed. Using basic colony assay and zymogram analysis (gel electrophoresis) to observe the expressed enzymes, molecular weight and gene size of the enzymes were revealed. With comparison to E. coli control strain, the related enzymes were only found in WLA12. To express the Bacillus genes in E. coli, molecular cloning and gene transformation via electroporation was carried out.
動力機械(內燃機)模型引擎之研究
最古老之動力機械為蒸氣引擎，在早期蒸氣火車發電機皆由蒸氣引擎發展而來，本研究即利用最古老之蒸氣引擎(俗稱飛龍引擎)之製作，以探討內燃機之基本理論以作為模型引擎設計之基礎。在飛龍引擎製作中，歷經引擎無法轉動之痛苦，並極力找尋一百年前發明蒸氣引擎之同樣思考，最後找尋到引擎啟動之合適尺寸，再用其尺寸延伸做實驗，以發展其理論，最後發現引擎設計之重要變數如管長﹑火焰﹑本身重量均為設計中重要因數，最後用正式機械設計方式完成第四代引擎亦正式運轉，可見本蒸氣引擎之設計基礎具有相當實用之基礎。The oldest locomotive engine was the steam engine. All early steam locomotives were developed from the steam engine (Heron engine) and this research project uses the Heron engine to explore the fundamental theories behind the internal combustion engine as a model for basic engine design. During the production stage of the Heron model, there were difficulties with engine rotation, and so efforts were made to find the same thought processes involved in producing the steam engine 100 years ago. The dimension appropriate for moving the engine was first found, and then the idea was extended with experimentation of the size in order to develop the theory of engine design. In the end, the length of the steam hose, the strength of flame, and the actual weight of engine were all found to be important factors of design. Finally, the fourth generation Heron engine was able to rotate properly using the proper engine design method, so it can be seen that the fundamentals of steam engine design has quite a practical engine design basis.
臉上真的有蟲嗎？~揭開蠕型?的真面目
I’ve heard a report on the internet that there are mites on the face, even a clean face, and I took an interest in doing a research. First, I found that on the face is Demodex, which lives and depends on human beings; besides, nearly seventy percent of people have these mites on their faces. To know more about the habit of Demodex, I sampled forty people of both sexes and different ages. The analysis, not correlating with sex and times of face washing, showed that more mites are found on the forehead than on other parts of the face, and there is more probability to find Demodex on the face of those who are older, those who have oily skin, and those who suffer from acne. I also observed that these mites are photonegative, often gather together in the hair follicles, and feed on sebum. Moved from the human body, Demodex is livelier in sesame oil than in other kinds of oils, but its life ends in about eight to ten hours and dissolutes at last. In addition, I tried to devitalize Demodex by various kinds of medicine, cleansers and cosmetics, but only those containing sulfer, eau de parfum, and the essence of rosemary or lavender are efficacious. 網路傳聞乾淨的臉上也有蟲，引起我探索的興趣，查探後發現是和人類片利共生的毛囊 蠕形?和皮脂蠕形?，初步調查顯示近70%的人臉上都有蟲。為了更了解蟲的習性，以年齡 與性別區分共在40 人臉上採集樣本，統整結果得知，額頭比其他臉上部位多、年齡越大、膚 質油的發現機率較大，但不受面皰多寡、性別、洗臉次數等影響；觀察後發現蠕形?以人類 的皮脂為食，經常群聚在人體毛囊中，離開人體後在芝麻香油中活動力較佳，但約8~10 小時 後就死亡分解導致無法繼續觀察其生活史，對光有明顯的負趨光性，最後試著用各種藥品減 低蟲的生命力，以薰衣草、迷迭香精油、毒藥香水和蜜花沉澱硫等較有效。
口琴簧片振動與氣流的影響
本研究主題在測量口琴簧片受到各種氣流因子影響後，所產生音色、音頻等變化之探討。在過去我們認為，一片簧片不論如何吹奏，其發出的頻率皆相同。但是事實上，演奏家控制氣流的強弱、方向、渦流等，便可吹奏出多樣的音頻。探討形狀因子對簧片頻率的影響，如：長度、寬度、厚度對頻率所造成的影響。自製口琴，利用變壓器控制送風機風速。探討氣流因子對簧片主頻之影響，利用各種不同的自製吹嘴，改變風速、角度、渦流…等，找出可能使簧片改變頻率的氣流因素。實驗結果發現改變風速會影響簧片主頻的變化，風速越大，頻率越大，為一條平滑線。但並非一直都會上升，當簧片頻率上升至某一極限，便無法再利用風速使頻率上升。例如實驗四吸音標準狀態下，風速大於8 Kt 後，頻率一直停在429Hz。在外加障礙物時(模擬吹奏舌頭時隆起)和標準狀態(正常零度入射)下頻率比較吹音和吸音有明顯的差異。吹音時，同風速下，其頻率比標準狀態高，發生音升；吸音時，同風速下，其頻率比標準狀態低，發生音降，具應用性。我們發現在頻率改變時，簧片的振動型態會有所不同，所以利用高畫素像機拍攝和電腦相位差算出簧片之曲折點至尾端的距離，發現頻率和簧片之曲折點至尾端的距離成正向關係。如實驗五中頻率從414 至419Hz，簧片的曲折點到振動端距離也明顯變大。我們發現吹嘴和口琴只要稍有一點空隙(大約在0.2cm 左右)，便會和完全吻合時有顯著的頻率差距(吻合後大約比有空隙低20Hz 左右)，此實驗頻率變化現象和現實壓音頻率變化極為相近。實驗過程中發現，改變簧片吹嘴的吻合程度，吹入口琴的風速相近，但頻率變化卻也有壓音的音頻變化。在實驗三加入各種氣流因子發現入射角度和標準情形差異不明顯，因此推論壓音的頻率變化和風力強度、入射角度關係不大，壓音主要為渦流所造成的現象。簧片振動模式改變，導致簧片振動頻率發生變化，且簧片的自然頻率不變。當壓音產生時，氣流在振動面造成妨礙簧片振動的抗力，但琴格內部同時也給簧片的風壓，使簧片產生一種非自然振動的頻率。The theme of the research is to explore the changes on its timbre and frequency after the harmonica reed is influenced by each kind of air current factor .In the past ,most people think no matter how to play the reed ,the frequency it produced was supposed to be the same. But in fact the frequency will be changeable under different direction, turbulent flow and air intension by the perform. First to explore the basic feature of harmonica reed, for example: The length, the width, thickness cause the influence on the frequency. To make the self-made harmonica, using the transformer control air feeder wind speed. To discussion the influenced caused by air current factors,and use each kind of different self-restraint to boast, change the wind speed, angle, turbulent flow ,in order to discover possible factors the reed causes to change the frequency of the air current factor. The experimental result discovered the change of wind speed can affect the change of basic frequency , the stronger speed cause the bigger frequency, It will be a curve. But it will not be rising continuously, when the reed frequency rise to some limit, it is unable to cause the frequency rise again by using the wind speed. For example experiment four sound absorption standard conditions, after the wind speed is higher than 8 Kt, the frequency continuously stops in 429Hz. To compare obstacle (simulation plays when tongue sticks out) and the standard condition (normal zero degree incidence) , comparison blows the sound agreement sound absorption to have the obvious difference. When blows the sound, under the same wind speed, its frequency is higher than the standard condition, has the sound to rise; When sound absorption, under the same wind speed, its frequency is lower than the standard condition, has the sound to fall. The harmonica terminology for presses the sound, extremely has the application. We discovered when frequency change, the reed vibration condition have differently, therefore use the camera photography and the computer phase different figures out the reed winding point to the end distance, discovered the frequency and the reed winding point relate to end distance is being connected. If tests five medium frequencies from 414 to 419Hz, the reed winding point is away from to the vibration end also obviously changes . The different reed vibration condition cause the frequency to change. Natural frequency is constant. When cause “bending” (the frequency is lower than the standard condition), the airflow make a force keep from reed vibration. But the chamber air pressure still drive reed. therefore cause the reed to give off not natural frequency sound
樂器聲波之探討(The Study of A Sound Wave on Musical Instruments)
演奏樂器時，是使發聲體產生駐波而發出各式各樣悠揚的聲音及音調；樂器主要分成振動體(發聲體)及共鳴器兩部分，依發音方式分為弦樂器(使弦振動產生駐波)、管樂器(利用空氣柱振動產生駐波) 及打擊樂器(利用板、膜或磚等彈性材料的固有頻率振動產生駐波)。聲音有三要素：振幅、頻率及波型，響度取決於振幅大小、音調與頻率有關、波型則由不同的頻率及響度組成。樂音多變的主因是音色及音調。音色是發聲體的發音特性，取決於該發聲體的聲波波型。音調即聲音的高低，與發聲體的振動頻率材質息息相關，頻率愈高，其音調愈高，而樂曲中的音階高低則是由音調高低所構成。樂曲的製作及演奏必涉及到律制，從駐波的產生、律制的探討、頻率的測量和琴鍵的振動模型建立與波形的觀察，我們使用計頻器、示波器及有限元素ANSYS 軟體、數學計算Mathematica 軟體，我們設計一系列實驗，企圖對樂器聲波操作技巧有更進一步的認知。從實地走訪樂器製造廠，了解到設計與改良仍是樂器工藝家重要課題，本文的實驗方法可提供大型演奏會現場調音、樂器調音師或樂器工藝家設計製造樂器時參考用，對於發展文化產業期待提供更經濟與實用的建議。;Playing musical instrument is to make sounding part produce stationary wave so as to give off various gentle sounds and tones. According to different modes of sound producing, musical instruments which comprise vibrator (sounding part) and resonator can be divided into stringed instruments (which vibrate the strings to produce stationary wave), wind instruments (which produce stationary wave with vibration of the air column) and percussion instruments (which produce stationary wave with natural frequency of boards, films or bricks). Three Essentials of sound include amplitude, frequency and waveform, in which the amplitude decides the volume, tones are related to frequency and the waveforms are composed of different frequency and volume. Various musical sounds are mainly due to different timbres and tones. Timbres, sounding characteristics of sounding part, is decided by waveform of the sounding part. Tone means pitch of the sound and is closely related to vibrating frequency of sounding part. Higher frequency makes higher tones and pitch of a musical scale is decided by different tones. Music composing and playing is necessarily connected with music temperament including producing of stationary wave, discussion of temperament, measurement of frequency, establishment of vibrating mode of keys and observation of waveform. We adopted frequency counter, wave inspector, ANSYS software and Mathematica software and designed a series of experiment to get further knowledge of technique of handling musical instrument wave. After visiting musical instrument manufacturers, we learnt that design and improvement are still the essential subjects for instrument craftsmen. Experimental method in his article can provide reference for on-the-spot tuning of large concert, musical instrument tuner and musical instrument designing and manufacturing by craftsmen, and more economic and practical suggestion for cultural industry development.
波動奇蹟—皂膜與皂水共振模式之研究
本研究探討肥皂膜與肥皂水的共振現象。在肥皂膜共振方面，我們以實驗探討皂膜的共 振模式與頻率的關係；並配合共振理論模型求出薄膜厚度，再與體積密度-厚度測量方法比 較。而又發現皂膜在共振時，皂膜保存時間較平常久，故進行皂膜生命期與頻率、強度的關 係。在進行肥皂膜實驗時，肥皂水滴落在喇叭上，振動出奇特的形狀，進而探討皂水共振的 特性並嘗試建立其數學模式。 ;The research is about the resonance of soap film and soapsuds. For the resonante of soap film, we tried to find out the relation between the resonant pattern and frequency by experiment; according to the resonante model, we measured the thickness of soap film, which was compaired with volume-dencity method. We found that the life-time of the resonant soap film is longer than the normal one, so we proceeded to study the relation between the life-time, frequency, and power. We observed special resonant pattern while the soapsuds fell down on the speaker. So we studied the characteristics of resonant soapsuds, trying to make the mathematical pattern of resonant soapsuds.
你喝下了多少？-台灣市售優酪乳乳酸菌生長力及抗酸性之探討
現今乳酸菌飲料風行，但是乳酸菌是否真能通過胃酸的考驗，到達腸道進行複製，利益人體？我們首先以市售乳酸菌粉（加拿大Rosell 公司，含二種菌，暫時命名為"小毛"及"小白"）為預測菌種，利用分光光度計測定乳酸菌於Thioglycollate 培養基中的生長能力（OD600）。小毛在pH 值 1、 3 、5、 7 時之生長力分別為0、 0.008 、0.682 、0.847 ，小白為 0、 0.015 、0.973、 0.636。若於培養基中添加不同濃度的螺旋藻熱分解物，如加入0.01%的添加物後，小毛在以上各種pH 值生長力分別為0.042、1.291, 、1.447, 、1.213 ，小白為 0.053、1.392、 1.531、 0.988，意外發現可大幅提升菌的生長力及抗酸力。再取台灣市售4 種廠牌優酪乳（以甲、乙、丙、丁代表之），分離乳酸菌，再於各種pH 值中培養。結果在pH 3 時，螺旋藻熱分解物僅對丙廠牌有效, 乙廠牌無效, 甲與丙則有無填加生長力都很差。在pH 1 時, 則對乙、丙、丁皆有效，故建議廠商慎選菌種，並於製程及成品中添加螺旋藻熱分解物。The yogurt is a popular drink. But whether the lactobacilli inside can resist the destruction of gastric acid and grow well in the intestinal tract is still questionable. We used pure lactobacilli powder (Rosell Company, Canada, containing two bacteria named in this report as "Little Hair" and "Small White") for pre-test. The growth ability in thioglycollate medium was determined by spectrophotometer (OD600). The results of bacterial growth at pH 1, 3, 5, and 7 for "Little Hair" were 0, 0.008, 0.682, and 0.847, respectively. Those for "Small White" were 0, 0.015, 0.973, and 0.636, respectively. After supplement with 0.01% of the boiled lysate of Spirulina algae (ProBio Biotech, Taiwan), growth abilities at pH 1, 3, 5, and 7 for "Little Hair" were 0.042, 1.292, 1.447, and 1.213, respectively. Those for "Small White" were 0.053, 1.392, 1.531, and 0.988, respectively. The algae extract amply promotes the growth and acid-resistance, especially at pH 3, of these bacteria. The lactobacilli isolated from four different products of yogurt in Taiwan, named as A, B, C, and D, were then tested as above. Results showed the supplement with the boiled lysate of Spirulina algae was very effective, at pH 3, for promoting growth of C, but not effective for B. Growth abilities of both A and D were very unsatisfactory with or without this supplement. At pH 1, algae lysate supplement significantly improved the growths of B, C, and D. Therefore, this supplement in culture and product for yogurt preparation was suggested.
凸多邊形完美分割線的尋找
1) First, we studied the properties of lines and segments that bisect a triangle’s perimeter. By observing the properties, we found a “revolving center” what we defined. We employed the revolving center in the construction with ruler and compass to make “triangle’s perimeter bisectors” that pass the points we desire. Later, we found out the “envelope\r curves’” equations of the “perimeter bisectors” on the triangle’s two sides are parabolic curves. Moreover, the focus of this parabolic is just as same as the revolving center. 2) The curves envelope of area bisectors formed a hyperbolic curves. By similar method of constructing a “perimeter bisector”, we can also construct an “area bisector”’ by using the hyperbolic curve’s focus. We accidentally found out that we can construct the tangent of the conic by using our method, too. Different from the information we found, It supplies a easier method to construct the tangent of a conic. 3) With the rules of constructing perimeter (area) bisectors, we can expand the method to constructing the “perimeter (or area) bisectors” of any convex polygons. 4) We call the lines that bisect the convex polygon’s perimeter and area at the same time the "perfect bisect lines”. Based on the properties of the” perimeter bisectors” and the “area bisectors” in our research, we found out that the” perfect bisect lines” pass the intersection of the” perimeter bisector’s effective segment” and the hyperbolic. Thus, we can construct the “perfect bisect lines”. Moreover, we proved the esistence of the “perfect bisect lines.”1. 首先我們先探討三角形等分周長線的性質，利用性質及觀察等周線的變化，我們找到可利用本研究所稱的「旋轉中心」，以尺規作圖的方式，作出「任意點的三角形等分周長線」。接著我們導出三角形兩邊上等周線所包絡而成的曲線方程式為一條拋物線的曲線段。進而發現上述的旋轉中心，即為等周線所包絡而成拋物線的焦點。2. 三角形兩邊上等積線所包絡出的曲線是一條雙曲線的曲線段。利用等周線的尺規作圖，我們找到同樣可利用焦點當旋轉中心做出等分面積線。意外的發現出圓錐曲線的切線作圖，皆可利用我們的研究方式(有別於已查出的文獻上記載)，較快速的作出切線。3. 利用三角形等周線(或等積線)的尺規作圖，可擴展到「過任意定點作出凸多邊形的等周線(或等積線)」。4. 我們將同時分割凸多邊形等周長與等面積的分割線稱為「完美分割線」。利用三角形研究出的等周線與等積線相關性質，我們找出完美分割線必通過同角的等周有效段與等積曲線段之交點。利用這結果可作出完美分割線。並進一步，我們證明出凸多邊形完美分割線的存在性。