佛手瓜卷鬚之向觸性及其參與蛋白質之探討
本研究利用佛手瓜的卷鬚探討向觸性的原理。本研究大致分為兩部份,一方面我們在卷鬚中發現了含量極為豐富的構造,此一螺旋狀構造分布於維管束中,且用雙縮?詴劑檢測後發現其含有蛋白質,且不具有運輸水分的功能;並發現此一構造的分布疏密,會影響到螺旋內側外側以及切割後片段泡溫水的彎曲方向。此外,在進行卷鬚蛋白質電泳的過程中,我們發現使用含尿素的緩衝液萃取蛋白質的效果最佳,1克的卷鬚乾重約可萃取到5毫克的蛋白質,且蛋白質總量會隨著卷鬚的成熟而遞減。利用軟體比對及質譜分析八個蛋白質點,得知此八點的蛋白質為:malate dehydrogenase, oxygen-evolving enhancer protein 1, oxyen-evolving enhancer protein 2, calreticulin, peroxidase, stromal 70 kDa heat shock-related protein, and AP2/ERF and B3 domain-containing transcription repressor。由此可知,向觸性為植物經過一連串訊號傳遞後,對外界刺激的順應。
A Novel Contrast-Enhanced Brain Mimicking Hydrogel for Testing Implantable Brain Electrodes
Paralysis is a debilitating disorder that does not currently have safe and effective treatments. Implantable brain electrodes can be used to read brain waves and convert them into a corresponding motor function to restore movement in paralyzed patients. Tissue deformation induced around the implant site is believed to reduce their viability through the foreign body response. Developing electrodes that minimize deformation is challenging because the mechanical aspects of deformation are not fully understood and non-animal tissue models for testing electrodes are unavailable. Development of pre-clinical models for in vitro testing of the mechanical properties of electrodes can lead to a better understanding of this prevalent problem. The objective of this study was to construct a novel contrast-enhanced, brain mimicking hydrogel using photopolymerizable polyethylene glycol (PEG) polymer that contains alginate microspheres with enclosed gadolinium (Gd) contrast agent. 1.5% alginate microspheres were constructed with enclosed Gd-DTPA-BSA contrast agent and successively added into 10% PEG. Then, this mixture was photopolymerized using a 5 mW/cm2UV lamp to result in a successful brain mimicking hydrogel. Rheological testing showed that its elastic modulus was approximately 1.5 kPa, which is similar to that of a normal human brain. The model is valuable because the presence of the contrast agent in the hydrogel resulted in distinct bright spots on the MRI. This can facilitate the visualization of tissue deformation caused by electrode insertion via comparison of pre-insertion and post-insertion images. This brain-mimicking model has the potential to improve understanding of neural deformation from electrode implants in order to assist patients suffering from paralysis.
A.L.E.R.T
My project ‘Avert’ (To avert and vertebrae combined) involves research into posture related back problems and a solution to help prevent back pain by maintaining correct posture. My solution is a chain-like structure fitted with 24 sensors that is sewn into a Skins compression top. When worn the chain is aligned with the user’s spine from the lower back to the base of the neck and moulds to the spine as it moves. As everybody’s back is slightly different it is important that the device can recognise the difference between what may be bad posture for one person, yet relatively normal for another. To solve this the device takes an initial reading of the user’s spine in correct posture and saves it as a reference measurement. Bad posture is defined by the device as angles that exceed the natural movement of individual vertebrae and their platelets. While in use the 24 sensors are continually measuring the shape of the user’s spine and comparing it with the original reference measurement taken earlier. If any measurement links to unhealthy posture a light will flash and an alarm will sound either aloud or through headphones until the posture is corrected. This device can be worn during light activity and non contact sports to encourage the user to maintain correct and healthy posture. The device can also record and save poor posture measurements that can be uploaded later to a software program I designed to draw the shape of the spine as a vector diagram. The device can also be plugged directly into a computer so that the user can see the shape of their spine as it moves in real time. I, along with others, tested my solution with many activities and found Avert to be a beneficial and reliable product as it successfully detects slouching and many potentially problematic spine shapes. Throughout my research I gained a thorough understanding about the potential damage bad posture can cause and the endless number of activities it occurs in. With further development I feel there is a huge potential for Avert to become a marketable product used not only for posture correction in everyday activity, but for many other purposes such as physiotherapy, biomechanics and athletic performance. Many people have approached me with praise and requests for the Avert system which is extremely exciting and also rewarding to know that my research has the potential to help people.
Carbon nanotubes as efficient nanosieve for controlled assembly of nanoparticles
In this work, techniques to explore the capabilities of multi-walled carbon nanotubes\r (MWNTs) in sorting nanoparticles (NPs) were presented. A droplet of a solution comprising of quantum dots (QDs) with various sizes was deposited on an aligned array of intertwined MWNTs. Photoluminescence (PL) and fluorescence microscopy (FM) revealed that MWNTs were effective nano-sieves that could effectively sort out QDs with a size difference of ~ 2.1 nm.\r Cadmium Selenide/Zinc Sulfide (CdSe/ZnS)core-shell QDs and Cadmium Sulfide (CdS) QDs were used to explore whether chemical properties of NPs affect the sieving capability of MWNTs. Further investigation on the effects of micro-patterning on the sieving ability of MWNTs was also carried out.PL and FM results suggested that micro-patterning could aid in separation of QDs and thus improve sieving capability of MWNTs. With the above findings, QDs emitting different colors as a result of size difference could efficiently be assembled onto the MWNTs en route to three-dimensional architectures with controlled assembly of NPs.\r Together with controlled laser power to remove desired amounts of QDs decorated MWNTs, a multi-colored display could be achieved. Further experiments were also carried out to determine the feasibility of introducing MWNTs as filters for NPs. Dilute solutions containing NPs such as gold colloid was run through these MWNTs filters by gravity. Field emission scanning electron microscope (FESEM) images of the samples showed that MWNTs were successful in trapping the nanoparticles. Explorations into the length dependent effect of using MWNTs as filters, suggested that 300μm MWNTs are better nano-sieves compared to 50μm MWNTs.
Cable Stripper
PURPOSE OF THE RESEARCH \r The purpose of this project is to provide a cost-effective and efficient way of \r stripping electrical conductors, with thicknesses of 16mm up to 70mm in diameter, \r of their isolation. The current methods that are available are unsafe and unpractical. \r Therefore this project determines a safe way of stripping cables and also provides a \r new product to improve the worker’s safety during the process of stripping cables. \r PROCEDURES \r The solution can be found by doing research on the types of cable isolations \r currently on the market. By talking to the workers who use these types of tools, and \r strip these types of cables on a daily basis, I can comprehend the problems posed by \r the present methods and provide a solution. \r DATA \r An electrical cable is commonly a conductive wire surrounded by a nonconductive, \r insulation sleeve. In order to splice two cables together or connect the \r cable to an electrical device, the conductive wire inside the sleeve needs to be \r exposed. \r Numerous tools have been developed for slitting and stripping the electrical \r cable in order to expose the said conductive wire. The simplest tool is a knife with \r which the user makes an annular cut in the sheathing. The end portion of the \r sheathing then is pulled away exposing the individually insulated wires and the bare \r ground wire. The knife may also be used to cut away a short portion of the \r insulation at the ends of the wires. During both operations the user has to be \r extremely careful, or else the knife blade may damage the insulation around the \r internal wires and even nick the conductor or he may injure himself. To do so the \r user must first cut away several inches of the plastic sheathing at the end of the \r cable. A short length of the insulation then is removed from around each end of the \r conductors. \r As an alternative to using a knife, various scissors-like wire strippers have been \r developed. Although such scissors-like wire strippers are effective for removing the \r insulation from individual wires within an electrical cable, they are not efficient for \r removing the sheathing from the end of the cable in order to expose the individually \r insulated wires. \r A disadvantage of using a conventional knife and with using the known wire \r stripper is that a cable having a relatively thick insulation sleeve is difficult to strip \r and thus prepare for connections, since conventional wire stripping tools and other \r conventional devices, such as knives or tools with enclosed blades are inefficient for \r stripping thicker cable jackets. \r A further disadvantage of the known wire strippers is that, its basic \r characteristic dictates that the degree of friction between the tool and the wire after \r the insulation has been ringed will be high. This occasions no particular difficulty \r when only a short length of insulation is to be removed from the end of the wire. \r However, when yards and yards of insulation are to be stripped, as may be the case \r when reel ends are to be prepared for scrapping, the conventional wire stripper \r generates so much friction that it cannot be efficiently utilised. \r CONCLUSION \r The developed product enables workers to effectively strip electrical \r conductors without any impeding danger to themselves or the risk of damaging said \r cable. It is cost-effective and saves a lot of time. After several tests of the product no \r problems have been encountered up to this point.