氫氣在燃燒後只會產生水而不產生溫室氣體之二氧化碳，可謂一種潔淨能源。 生質能源是屬於碳中性(Carbon neutral)型之利用方式，因此本研究著眼於如何建構一個操作簡便的共代謝系統，將生質料源從微生物之發酵反應中釋放氫氣出來。 實驗的主要方法是利用好氧性的Bacillus thermoamylovorans 與厭氧性的Clostridium butyricum 共培養分解廢紙漿以生產氫氣。廢紙漿是混合的基質，內富含纖維素、並含一些油墨及少許雜質。利用Bacillus thermoamylovorans 是好氧菌，同時也能將廢紙漿中的纖維素轉換成還原醣的特性，將原本有氧的環境轉換成絕對厭氧的環境，並將廢紙漿中的纖維素轉化成Clostridium butyricum 可以利用的還原醣。如此一來，原本不利於Clostridium butyricum 生長的環境，卻能透過簡單的共培養方式創造出有利於Clostridium butyricum 生長的環境並產生氫氣。除此之外，我們也對不同碳源、不同的植菌量、不同的氧氣量，比較其產氫能力差異，發現增加氧氣量可以提升最後的產氫量大約2.7 倍。 ;Our major goal is to develop a cost-effective biohydrogen production system by the co-culturing of Bacillus thermoamylovorans and Clostridium butyricum. The aerobic Bacillus thermoamylovorans will consume oxygen and converse waste paper pulp into reductant-sugar and the anaerobic Clostridium butyricum will generate hydrogen after oxygen is consumed. With the increase of aeration, the aerobic Bacillus thermoamylovorans growsappropriately leading to more biohydrogen production. However, in enhanced aeration condition, the Bacillus thermoamylovorans will consume sugars that can offer for the Clostridium butyricum. So we can conclude that the control of oxygen is the key point for the system to operate.

含羞草會因為受到各種不同外界刺激(光照、外力等等)，而造成膨壓改變。外 觀上表現出葉片閉合或有葉柄下垂的現象。其中含羞草處在黑暗的環境下一段時 間後，會做出睡眠運動。當它重新暴露於光照之中，將會需要一段時間以恢復原 先葉片張開的樣子。這個實驗是研究不同類型的環境因子(主要是光照和溼度)在打 破睡眠運動之後，對其葉片復原時間所造成的影響。我們針對上述環境因子在不 同狀況時，進行我們的實驗上百次，進一步得到了多項的數據。也在實驗中，為 了精確了解整個恢復的程序，而將恢復程序程序做成書面說明。以下是我們所紀 錄與分析完數據後的結果，以及我們在實驗期間，進行一些不同的實驗嘗試，所 發現不同於表面所見的驚人事實。 The mimosa can accept plenty of stimulation (light, force, etc), which results in the change of turgor pressure, and on outward appearance, it shows the phenomenon that the leaves become closed or that the stalks get pendent. Among the stimulation, the mimosa will undergo nyctinasty when it is left in the dark for a period of time, in order to enable it to be exposed to the light once more, which requires certain time. This experiment is based on how different kinds of elements of the environment (primarily light and hydro level) effects the rehabilitation time after the nyctinasty is broken. A large number of data are gained after experimenting on it for hundreds of times. Steps of habilitation are also made into illustration in writing, so as to understand the whole steps accurately in the experiment. Below are our records and analysis based on the data, including a few special experimental tries during our working time, in which some surprising facts that were discovered are different from what are seen on the surface.

陸地上的金字塔無時無刻沒有受到氣體的包覆，這個實驗透過風洞模擬金字塔模型，在流體中的壓力變化，並藉由煙線來觀察流體的移動情形，可具體看到當流體經過物體週遭時流場的改變。經由測量壓力，可更深入的探討風速與壓力的關係。在實驗中，可發現當流體迎面而來，在金字塔後方形成的流場變化中，以渦流最易觀察；且透過壓力的測量，發現模型的前、後方，會有明顯的壓差，會對模型造成力矩，可能會使金字塔結構不穩定。越往金字塔上方壓差越小，因此其受阻力所產生的合力矩應較同底面積、同高的長方體小，故金字塔可以長久在沙漠中屹立不搖。此外，流體流過模型兩側會產生分離的情況，分離點的位置會影響渦流區的大小；一般而言，分離點發生在物體的越後方，尾流的尺寸越小，壓差所造成的阻力越低，實驗中發現流速對分離點的位置影響不大，金字塔的擺放形狀卻會對分離點的位置產生明顯的影響，所以越流線型的物體，其受到壓差所產生的阻力越小。希望透過以上的研究，能夠對金字塔周圍複雜的流場有更深入的了解。The pyramid on land is constantly surrounded by gas. Using the wind tunnel, we can investigate the distribution of pressure surrounding the model of pyramid; furthermore, we can discuss about the relationship between pressure and wind velocity. In addition, with the smoke wire, we can visually observe the dynamic flow filed. In our experiment, we discovered that when fluid comes, there will be obvious difference of pressure between the front and the back of the pyramidal model, causing a torque to the model. At higher parts of the model, there is less difference of the pressure, so the resulting torques of a pyramid should be less than that of a cuboid. Therefore, a pyramid can stand in the desert for a long time. Besides, when the fluid flows through the two sides of the model, it will separate, and the position of the separation points will influence the size of the turbulence area. Generally speaking, the farther the separation points are to the back, the smaller the size of the turbulence area, and the less the drag caused by the difference of pressure. With the study above, I expect to have more understanding about the complex flow field of pyramid.

本研究主要是以台北市中心的氣溫觀測站，以及市區外圍東邊的汐止，南邊的屈尺，以及西邊的山佳等三個氣溫觀測站，從1998 年至2004 年的七年期間的氣溫資料中，來探討台北地區的熱島效應現象。這個研究的結果發現，台北市區與周遭鄰近地區確實會因為熱島效應的影響而產生1~2℃的溫差。這個溫差在白天時不甚明顯，在中午過後，便由台北市中心逐漸向郊區遞減，形成類似同心圓狀的分布。溫差最大值發生在夜晚，使得台北地區晚間宛如一座夜晚增溫的城市，同時夏季的熱島效應又較冬季顯著，氣溫又逐年遞升，造成夏季台北市區的高溫屢創新高，將是未來都市發展的危機。This study is focused on the urban heat island effect of Taipei Basin, northern Taiwan. The hourly temperatures of Taipei meteorological station and three rural sites eastern Hsi-Chih, southern Cyu-Chih and western San-Chia, were compared from 1998 through 2004 to illustrate the temperature differences between city center and surrounding country areas. The results show that a difference of 1~2℃ exists between city center and surrounding country areas, indicating the extent of urban heat island effect in the Taipei Basin. The daily temperature contours show a clear high-temperature bull’s eye at the city center during the midnight, implying the high latent heat trapped by the dense and high rise buildings. This phenomenon is not sharp in the high noon due to the reduction of temperature difference between city center and surrounding country areas. The heat island effect is most prominent in the summer than that of the winter. Along with the increasing temperature-difference trend through years, the summer times often experience record-breaking heat waves and pose great risks for the city development in the future.

本研究主要以正三角形作為基本單元，透過窮舉討論得到正三角形邊的作用方式只有五種，再經由排列組合歸納出11 種正三角形密鋪磁磚設計方法。進一步，運用我們的研究結果，配合數學簡報系統製圖，創作新圖樣，也彌補了Escher 在手繪時所造成的誤差，達到完全密鋪的效果。碎形磁磚的部份，我們也依據其背後的數學理論創作幾套結構圖，利用結構解析，碎形密鋪磁磚將變得十分容易，學習者將可輕鬆製作富有創意的新圖樣。 ;This research mainly takes the regular triangle as the basic unit. Through the enumeration, we obtain that there are only five operations for edge of the regular triangle, and then 11 kinds of regular triangle design methods are induced. Even more, utilizing our findings and Mathematical Presentation System (Math PS), we created the new pattern which makes up Escher’s errors and achieves the tiling. As to Fractal Tiling, we create several sets of structure drawings according to its mathematics theory. Using structure analysis, the Fractal Tiling will become extremely simple, and the learner can make the rich creative new pattern easily.

豆類澱粉?抑制劑具害蟲防治與血糖調節之功效。本研究從台灣五種豆類中進行澱粉?抑制劑活性初篩，篩選出四季豆對麗蠅的澱粉?有明顯的抑制效果，粗萃後分離純化出單一蛋白質，經胺基酸定序結果得到兩個多?片段-VGLDFVLV 與TETSFNIDG ， 與已發表文獻比對推測為腰豆澱粉?抑制劑—αAI-1。經測試發現此抑制劑在85℃時仍具備抑制果蠅澱粉?之活性，為一熱安定性蛋白質，其抑制作用受pH 值影響很大，在偏酸性環境下的效果最好，且其抑制作用具特異性，可明顯抑制果蠅、入侵紅火蟻、白蟻、蟑螂及麵包蟲等昆蟲的澱粉?活性。在調節血糖方面，本研究利用豬胰臟澱粉?進行抑制活性測試，篩選出四季豆、花豆與黑豆對豬胰臟澱粉?有明顯的抑制效果，進一步利用Wistar 品系大鼠，進行豆類澱粉?抑制劑降血糖功效之活體試驗，將四季豆、花豆和黑豆粗萃經濃縮乾燥後餵食大鼠，發現花豆、黑豆在第一小時具有顯著抑制血糖增加效果；四季豆、黑豆在第三小時血糖回升，花豆仍可維持較低血糖濃度。此初步結果顯示，花豆粗萃物可能具備減緩消化吸收作用，具應用潛能，其確切分子作用機制值得深入探究。Amylase inhibitors can be applied in pest control and glucose adjustments. The study screens Phaseolus vulgaris from five Taiwan’s beans that has the most significant inhibition towards Chrysomia megacephala. The protein was isolated and sequenced two fragments, -VGLDFVLV and TETSFNIDG, highly homologous to that of αAI-1 from Phaseolus vulgaris. The pure protein still inhibits the amylase from Drosophila melanogaster at 85℃, suggesting it is thermal-stable. Its activity was specific and was affected by pH, reaching the peak in weak acidic condition. It obviously inhibits amylases from D. melanogaster, Solenopsis invicta, Odontotermes formosanus, Periplaneta Americana Linnaeus, and Alphitobius sp. In addiction, the study points out Phaseolus vulgaris, Phaseolus coccineus L. and Glycine max, which have significant inhibition toward pancreatic amylase. The amylase inhibitors’ functions of decreasing in blood glucose were detected by animal experiments on Wistar rats. The rats fed with the extracts of Phaseolus coccineus L. and Glycine max showed the inhibition of glucose increasing at the first hour, while blood glucose concentration after rats fed with Phaseolus vulgaris and Glycine max increase at the third hour, and blood glucose after rats fed with Phaseolus coccineus L. maintains low. Its premier result indicates that the extract from Phaseolus coccineus L. might postpones digestion and has potential to be applied. According to these results, amylase inhibitors are worthy to further analysis.

直接乙醇燃料電池以酒精與氧氣透過氧化還原反應產生電能，但化學反應緩慢，需利用觸媒以增加其速率。本實驗目的在於盡可能找出一個表現最佳的觸媒。本實驗利用活性碳粉作為觸媒（鉑、錫）的載體，以酸性（HNO3）與鹼性（NaOH）環境分別處理碳粉，再以含浸法與多元醇含浸法將觸媒還原。我們得到以HNO3 處理的碳粉無法保有原碳粉的型態，較利用NaOH 處理為差。在觸媒製備方面，多元醇含浸法還原效果比含浸法可得到較小的觸媒尺寸，在本實驗中，溶液中鉑與加入的碳粉重量比為3:7 時，可得到最大的反應面積。此外，當鉑與錫原子數比為4:1 時，可得到最大的乙醇氧化電流。Direct ethanol fuel cell is a kind of power source which generates electrical power by a redox reaction involving ethanol fuel and oxygen. However, this reaction takes place slowly; therefore, catalyst is needed to improve its activity. The goal of this project is to get an optimize catalysts ratio to obtain the best catalyst activity. Activated carbon is used as the support of catalyst (platinum and tin) particles in this project, which is pre-treated in acid (HNO3) and alkaline (NaOH) solutions respectively. Then, the precursor is reduced by impregnation and EG-impregnation. We learned that activated carbon pre-treated with NaOH activates better than which pre-treated with HNO3 because the latter bear less resemblance than the former. As for the catalyst, the results of EG-impregnation show smaller size of catalyst particles than those of impregnation. In this project, when the ratio of the weight of platinum and activated carbon added into the solution is 3:7, we can get the largest surface area. In addition, when the ratio of the amount of platinum and tin atoms is 4:1, we can get the largest current of ethanol oxidation.

在炎炎夏日總希望來點可以「降火氣」的食品，依據古籍記載某些中藥材具有降火氣的效應，而中醫所謂的降火氣與西醫的抗發炎反應密切相關。本實驗利用巨噬細胞株受到LPS (Lipopolysaccharide，革蘭氏陰性菌細胞壁表面所含有的脂多醣體)刺激後，會分泌發炎介質的現象，再分別加入各項傳統認為可降火氣(以菊花、薏仁、仙草作為樣品)或上火氣(選用龍眼作為樣品)的中藥食材，來評估中藥材對巨噬細胞分泌發炎介質能力的影響。經由實驗，我們發現市售的『降火氣』中藥食材，在適當的濃度下，確實具有降低IL-6、TNF-α 分泌量的能力，但濃度過高時卻會造成細胞凋亡。另外，傳統以水熬煮的方式比冷凍乾燥取得的樣品有更好的降低IL-6、TNF-α 分泌量的能力。 我們期望以此一系列的實驗，來建立篩選『降火氣』食材的抗發炎效用的研究模式，並在後續實驗中可用以篩選出更多具抗發炎效應的中藥材。In summer, people always consider eating some special things to refresh themselves. According to ancient books, there are some Chinese herbal medicines that were recorded to have the function of cooling us down, which is related to anti-inflammation in the Western. Macrophage cell lines can be stimulated by LPS (Lipopolysaccharide) and secrete proinflammatory mediators. Thus, it is often used to evaluate the anti-inflammatory effects of different herbs. In our experiment, we also use macrophage cell lines to test different anti-inflammatory effects between chrysanthemum, Job’s tears and grass jelly. In addition, some Chinese herbs such as longan that can parch us are also tested in our experiment. In our results, we found these herbs that are available indeed have ability to anti-inflame in appropriate consistency; however, high dose does harm to cells. In addition, the samples that are poached in ancient ways have better ability to show the low dose of IL-6 and TNF-α. Finally, we would set up a model to test the anti-inflammatory ability of different herbs that were said to be able to cool us down, and then we could test more herbs in the future.

政府最近公佈在東沙群島設立第一個國家海洋公園，顯示國人重視海洋資源的保育與復育；但是東沙島特殊的自然景觀「會擺動的沙嘴」—龍擺尾，卻因興建八座消波塊構築的突堤而消散停擺。本研究以一個高中生的觀點，設計及進行一系列的模型實驗，探討東沙島的形成機制、各種環境因素對海灘及龍擺尾的演化、驗證龍擺尾的現況及如何讓龍擺尾復活，再由東沙島海灘復育看世界的海岸問題。雖然設備很克難，但都能完成預定的目標，證實東沙島的海灘演化。希望此次對東沙島海灘的研究成果與初步探討，能應用於世界上遭受侵蝕而需復育的海岸。In 2007, our government proclaimed Dongsha archipelago as the first national marine park, signifying her emphasis on the conservation and restoration of marine resources. However, the sand spit on Dongsha island, a unique natural wonder commonly referred to as the “swaying dragon tail”, has become motionless since the construction of a total of eight groins along its SE and NE coast during the period between 1993 and 1996. This study has conducted a series of model experiments to verify several fundamental issues, ranging from the formation and the factors affecting the stability and the status quo of Dongsha island and its spit. The experimental results find several favorably outcomes, giving the coast evolving evidences of Dongsha island. Furthermore, strategy to stabilize the coast and revitalize the spit are then considered, which could hopefully benefit the coast suffering erosion on the world’s coastline.

全球暖化造成水災頻繁，嚴重威脅植物生存。看似耐淹水的水稻，在完全淹水下亦有其生存危機。水稻FR13A 因耐水性極佳而常用於分子育種，IR64 產量高卻不耐水。是那些特質使稻種間有不同耐水機制？我們觀察其幼苗淹水24 小時後生長情形、通氣組織 (aerenchyma) 變化及應用即時反轉錄聚合?鏈反應 (real time RT-PCR)，研究酒精醱酵的主要蛋白質：乙醇脫氫? (alcohol dehydrogenase, ADH1, 2) 及丙酮酸脫羧? (pyruvate decarboxylase, PDC2) 基因之表現量。兩種水稻的胚鞘及根都因淹水減緩生長，以FR13A 減緩最明顯。通氣組織在淹水期間都有增加，FR13A 中的形成近似於對照組，IR64 則明顯較差。FR13A 中ADH1 及ADH2 在淹水一小時後迅速增加60 至100 倍，IR64 僅增加10 至20 倍。PDC2 在IR64 中表現量的增加幅度較大，但最大值仍小於FR13A 之基礎表現量。由此可知，FR13A 在完全淹水時成長減緩而原有通氣組織則持續生長，且酒精醱酵中的基因有獨特誘導反應，因此耐水性較佳。藉由此研究揭開水稻細胞及分子生物學上的耐水反應策略，將可更精準地改良稻作使其對抗淹水逆境，解決未來因環境造成的糧食危機。Global warming increases the frequency of flooding, which drastically reduces the growth and survival of plants. Although rice (Oryza sativa) appears well-adapted to flooding of roots as it is often farmed in paddies, problems arise when the whole plant is submerged in water. I am interested in the structural and molecular responses that result in different submergence tolerances in rice cultivars. Indica rice FR13A is submergence-tolerant and frequently used in molecular breeding for this trait, while IR64 is a high-yield but submergence-intolerant cultivar. In this study, I monitored the growth rate, aerenchyma formation, and gene expressions of the carbohydrate metabolism in FR13A and IR64 seedlings subjected to submergence for 24 hours, by means of real time RT-PCR and microarray. FR13A had prominently inhibited coleoptile growth and sustained levels of aerenchyma formation whereas IR64 did not. The mRNA levels of alcohol dehydrogenase 1 (ADH1) in FR13A was induced prominently, while ADH2 was induced in IR64 during early hours of submergence. The induction of pyruvate decarboxylase 2 in FR13A was stronger than IR64. The expression of sucrose synthase was similar in both strains. Expressions of the genes involved in anaerobic carbohydrate metabolism were also studied by analyses of microarray data. My findings demonstrate that elongation quiescence, persistent aerenchyma formation and shifts in anaerobic carbohydrate metabolism gene expressions are beneficial strategies of FR13A towards submergence. Through elucidating the molecular basis of coordinating submergence tolerance genes as this study provided, it will be possible to discover multiple traits associated; hence crop improvement for flooding tolerance could be achieved.