Recycled PET bottles for vacuum packaging
Vacuum packaging is a packaging technique intended to extend the shelf life of food via the removal of air from an enclosed package prior to sealing. This process limits the growth of aerobic bacteria or fungi due to oxygen deprivation. In this work, we present a novel do-it-yourself vacuum packaging device using the exchange of water and air between two bottles to continuously generate a vacuum-suction effect. The sizes of bottle and vacuum bag were investigated for its impact on the vacuum generation in a plastic bag containing smoked fish sausages. Large commercial 3.1-litre PET bottle generated more vacuum than the smaller ones. An equilibrated vacuum pressure of a smaller plastic bag was lower than that of a larger size. With 3.1-litre PET bottles, the vacuum pressure for 3”x5”, 5”x8” and 6”x9” bags was equilibrated at 8, 10, 18 mmHg, respectively. Sausages packaged by our device last for 14 days when they were kept in -20oC refrigerator, which was comparable to those packed by the commercial vacuum packaging system for household use. This project demonstrates an application of simple science in a real life situation as well as a promotion of environmental protection idea as the electricity is not used in the vacuum generation process and the disposed plastic bottles can be reused.
Research the efficiency of the fog-catching nets
Islands far from lands use the underground or surface water as the water for living. The population of the islands is growing fast and the amount of water usage is increasing year after year. However, the amount of water usage is limited, so that people who live in islands have trouble using water. To compensate this problem, underground water is drawn from deeper underground sites. If this matter occurs continuously, sea-level may rise and then we cannot use underground water. Seawater desalination is a way to solve the water shortage, but it requires a lot of energy. It is difficult for island far away from lands to supply a lot of energy. It is considered the eco-friendly way to minimize the use of energy on the island. In order to solve the problem of water shortage on the island, it is considered fog that on the island occur frequently. It is an attempt to create water from fog, but it is a lack of research of efficiency of fog-catching nets to create water from fog. In this research, I have studied the efficiency of the fog-catching nets, a way to increase the efficiency, the amount of water that is created on the island, usage of discarded fishing net for fog-catching nets. Through this research, I found a kind of fog which can be changed into water and the difference in efficiency due to the difference in the size of the mesh size of the fog-catching nets, wind direction, wind speed, water absorption capacity of thread of fog-catching nets, installation direction of fog-catching nets, a way of installation of fog-catching nets. Also I found fog-catching nets of discarded fishing nets on the island and the possibility of usage for everyday life that the amount of water are created for a day or a month during dry season on the island.
Smart Washer
Data and records show accidents caused by loose bolts or nuts often occur in building or mechanical structures all over the world. They may be train derailments, parts falling off amusement rides, escalator breakdowns or wheels coming off automobiles. These incidents can often cause serious casualties and should not be ignored. At present, the only devices used to prevent screws loose are spring washer and nylon locking nuts, but they are not readily detectable with the naked eye when they failed to tighten. Based on simple mechanics and spring principled, our “Smart Washer” has been designed to detect loosen screws. Whenever the bolt or nut gets even slightly loose, the lower part of the washer will spring up, this is a sign to alert and remind the user to carry out maintenance and re-tighten the loosen screw before serious accidents occur.
Expression of TRPV5 in Astrocytes: Implications for Ischemic Stroke
Elevation of intracellular calcium secondary to increased calcium influx along with increased gliosis are implicated in the pathogenesis of focal ischemic stroke. In astrocytes, which play a major role in maintaining homeostasis in brain ischemia, the identities of the ion channels responsible for increased calcium influx during ischemia is relatively unknown although several Ca2+-permeable transient receptor potential (TRP) channels have been identified to have contributing roles. The transient receptor potential vanilloid 5 (TRPV5) channel is a Ca2+-permeable cationic channel expressed primarily in kidney epithelial cells and at low levels in the brain, but the exact localization and role this channel plays in the brain has not been explored. To investigate the possible role TRPV5 plays in astrocytic calcium influx in ischemia, we examined the functional expression of TRPV5 in astrocytes subjected to hypoxia-ischemia in vitro and in rat models of ischemic stroke in vivo. We hypothesize that TRPV5 contributes to increased calcium influx in ischemia. By treating astrocytes with culture conditions without glucose and with low oxygen levels, we found that TRPV5 is upregulated with increasing durations of simulated hypoxia-ischemia in vitro. Similarly, rat models of ischemic stroke with middle cerebral artery occlusion also show TRPV5 upregulation in reactive astrocytes, suggesting a possible role of TRPV5 in reactive gliosis in vivo. Microfluorimetric intracellular calcium imaging using Fura-2 on primary cultured astrocytes show a voltage-independent increase in astrocytic calcium influx after hypoxia-ischemia in vitro that is selective for extracellular Ca2+ concentration and is reduced by inhibition of TRPV5 with ruthenium red. Electrophysiology measurements using the whole-cell patch clamp technique on primary cultured astrocytes reveal a non-selective cation current similar to that of TRPV5 that is inhibited by Mg2+, another inhibitor of TRPV5. Preliminary results on astrocyte cell viability during hypoxia-ischemia with TRPV5 inhibition by ruthenium red also suggest that inhibition of TRPV5 could enhance astrocyte survival and reactive gliosis in vitro, indicating a beneficial role in blocking non-selective Ca2+ entry via TRPV5 into astrocytes. Since TRPV5 is highly selective for Ca2+ and an important channel for Ca2+ absorption in various epithelial cells, TRPV5 upregulation may contribute significantly to elevated Ca2+ influx in astrocytes in hypoxia-ischemia. Also, Ca2+ influx has been demonstrated to play a crucial role in reactive gliosis, further suggesting that TRPV5 upregulation is involved in reactive gliosis. We propose that TRPV5 is involved in ischemia-induced calcium influx in astrocytes, and might participate in the pathogenesis of focal ischemic stroke.