日長處理對大豆蛋白質合成的影響
日照影響花芽分化及生長試植物生理學上的重要課題,根據相關文獻指出,蛋白質類型的變化是影響開化的重要關鍵,大豆是短日照植物,分別以長日照(LD18hr/DAY)和短日照(SD6hr/DAY)處理,抽取葉片中的蛋白質以SDS-PAGE.2D-SDS-PAGE分析,SDS-PAGE的實驗姐果顯示出在分子量25.2 32.9 38.6 20.6 64.0 100.6 kDa處中的蛋白質SD比LD的量多,但是在分子量16.8kDa以下的蛋白質LD含有高濃度而自SD只有一點,我們使用2D-SDS-PAGE做進一步的分析,SD與LD分為三個區塊討論(SD1-3 LD1-3)有許多蛋白自LD1.LD3出現,但在SD1 SD3中未出現,可能有抑制花芽分化的作用,而在SD2中有多個高濃度蛋白出現,可能在花芽分化上扮演重角色,其中編號P1 P2的蛋白質有較明顯的變化,根據NCBI網站上的資料顯示,P1蛋白質與光敏素C有關,而P2蛋白質則沒有找到分子量等電點類似的蛋白質;The effects of photoeriod on floral initation and develoment is an imporant phyiological subject and have studed ve he past 70 years. Accrding to the reference, polypeptide partten plays an important rolein photoperiodic induction. Soybean s a SD(short day)plant. So we planted them with 6-h(short day,SD)and 18-h(long day,LD)photoperiods. Total proteins n leaves of soyban were extracted from the vegetable,SD and LD. And using SDS-PAGE and 2-D eloctrophoresis to analyze. 25.2,32.9,38.6,50.6,64.0,and 100.6kDa polypeptides in SDS-PAGE were always present in leavess at SD but only a little bands f the polypeptides appeared at LD. And many polypeptides whose molecular weight(Mw)is below 16.8 kDa had higher concentration at LD ad Reduced at SD. We found three different areas(SD1~3,LD1~3) and analyze them. Many proteins were found n LD1,LD3 and reduced in SD2,SD5. Possibly, these polypeptides found in LD1 and LD3 play an important role in restraining floral iniiation in soybean. Many proeins had more concentration in SD2. Probably, they play an important role in photoperiodic induction and floral initiation. We found a protein labeled P1 in SD2 and it is with molecular weight(Mw) of 36kDa at isoelectric point(pI) of5.2, and it may be related to cytochromeC. We also found protein labeled P2 in SD2 and it is with molecular weight(Mw) of 40.2kDa at isoelectric point(pI)of 5.4, and we couldn't find any protein that has the similary Mw and pI in potein database about flowering and daylength.
應用垂直循環層流淨化水質之研究
The results of the thermal diffusion experimental show that the separation efficient\r increases with increasing ΔT, but decreases with increasing Q at C0 constant. When\r Q is kept constant, the efficient of change ΔT are small, and the maximum at C0 about\r 545 mg/L. The purification efficient of raw water for thermal diffusion is low.\r And it is high energy consumption, difficult operation, and high costs. So the potential\r of the application is low. Therefore, the vertical circulatory laminar flow apparatus\r was developed in this study. And the separation equation of this apparatus was also\r set up. The results of vertical circulatory laminar flow experimental show that\r the efficient increase with increasing V, and the optimum conditions are at about\r Q= 1.05 L/min, C0=800±50 mg/L, and V=26 cm/min. The separation efficient of the\r vertical circulatory laminar flow apparatus is higher than thermal diffusion apparatus.\r And its energy consumption and costs are low, and easy to operate. Since all interior\r moveable plates have two operation sides, so the multiple channel apparatus is application.\r Therefore, the multiple channel apparatus can treat large amount raw water and has\r low costs. The efficient of the experimental all are better than the theoretical\r values that may due to: (1)the assumed partical diameter is too small,(2)the flow\r is not real laminar flow, and (3)the end-side effect is not considerate.
\r 熱擴散實驗結果顯示在固定C0下,分離成效隨ΔT增加而增高,但隨進料流率增加而降低。在固定ΔT下,分離效率亦隨進料流率增加而降低。固定進料流率下,增加ΔT對分離效果的影響不大,以C0=545±10\r mg/L時,顯現出最佳成效。採用熱擴散裝置雖對降低原水中之懸浮微粒含量效果有限,且能源需求高,造成高成本和操作複雜,不具實用潛力。為提高分離原水微粒之成率,以及簡化操作與降低成本,本研究開發垂直旋轉帶裝置來製造出上下循環水流,並導出水流與微粒於其中之流速分布式,以及頂部、底部出口之微粒濃度計算式。垂直循環轉帶實驗結果,顯示提高V值有助於降低Ct/Cb比值;Q=\r 1.05 L/min左右、C0=800±50 mg/L下與V在26 cm/min左右,均有較低之Ct/Cb比值。垂直旋轉帶裝置分離成效明顯優於熱擴散裝置,且設備簡單、能源需求低與操作容易。由於旋轉帶之兩面均可以做為移動平板,因此不僅可以用來處理大量之高濁度原水,也能更具經濟效益。由理論與實驗結果之比較,顯示理論成效較實驗差,探討其原因可能是:(1)平均粒徑之假設值偏低、(2)流場並非全然以層流流動、(3)兩端終端效應未加考慮等。
形?與形外
在這篇研究報告中,我用了三種觀點來推廣幾何中的反演變換,首先,把反演變換視為是一種圓內與圓外的一種1-1且onto的映射,第一種推廣,是將變換中心移到視圓心以外的圓內的地方,馬上我們得到一個結論「反演半徑會隨著動點而改變」,接著,我們實驗了一下反演變換用有的一些性質,保角性,保圓性,…等在這個變換視中是否依然存在;接著我們用第二種方法來推廣反演變換,我們將邊界的形狀由圓視改成別的形狀(如三角形,四邊形…等等),然後也試試看在這種變換之下是否還擁視有反演變換的一些性質;第三種推廣,則是在研究的過程中,我發現了一種新的幾視變換,承接第一種推廣,我們將原先為定點的變換中心改為動點,將原先的動點改為定點,做出來的一種新變換。In the study, a new geometric Inversive transformation through three points is discovered. Here is the main result:(1)The first, onto cycle of inside and outside can be proved under invasive transformation. It is changed moving the center from center of cycle, we can get a new ” Inversive radius can be changed by moving drop. (2) We hope to find the answer to this problem by experiment, it is exist with the inversive properties. (3) A new geometric transformation is discovered, a fixed drop can be changed moving drop, then the first moving drop shifted the fixed drop. This leads to a new construction if the new transformation.
Parallelize it! 運算分享與系統自我校調
The research is about the optimal on parallel processing. Through boot disk – which will automatically finish booting configuration, .it is efficient and quick to build high performance PC clusters. The advantage of parallel computing could be applied to massive image processing. By sharing processing and breaking huge processing load into lots of pieces, we could get more efficient result. It is also possible to optimal parallel system through some special means such as dynamic configuration. Through the means, the system could distribute work loading itself. It could also adjust itself to get the highest performance and the most stable environment. 本研究之目的在於探討平行處理中的計算資源的最佳化,透過自動完成開機設定的Boot Disk 來有效快速建製出高效率的PC Clusters 環境,並透過動態配置與類神經網路的校調,使整體叢集的運算能自動調整至最佳化。平行處理優勢,可以應用在耗費極大量的運算資源的影像處理上。透過運算資源分享,可以以很高的效率將極為龐大的運算工作分散成許多較小的程序,使影像處理速度加快。經由平行演算法及實際應用的調整,可對已成形之平行系統作效能上的加強。使用類神經網路的方式訓練,使其系統能夠自我分配運算工作量,且隨著各平行化程式與各節點的不同,能自我校調至最佳化,達到高效率且穩定的運算環境。\r 本研究透過高效率且能自我調校的運算環境,可用於優化其本身結構,以達到\r 演化出更進一步系統,具有相當大的發展潛力。