全國中小學科展

2002年

平面上三點集中度判別法之探討

關於平面上若干點的集中度之定義,一直很少有人予以仔細的探討,因為判別的方法有很多種。本研究是以平面上三點所構成的三角形之三頂點作討論,分別以三角形的面積、周長,內心、外心和重心至三頂點距離,三點的標準差及平均差的概念,作為不同的判別方式,並以動態幾何化版GSP模擬不同的判斷方法做為研究,在利用統計學上的方法,找出哪一種定義方式最為適合、誤差最少的判斷方法。There are many ways to determine the centralness of three dots on a plane; however, no definition study has been applied. In this study, we focus our interests on the entralness of three vertices of a triangle formed from three dots on a plane. Various methods such as the relationship of area, and the distance of the triangle to the centralness of three dots, the distance from the interior point, exterior point, the barycenter to the vertices of triangle, and the standard error and differences average obtained from three dots were determined with a dynamic geometry software GSP, and a statistic method was used to find a least error way to determine the centralness of three dots on a plane.

Gene Targeting Using Chimeric RNA-DNA Oligonucleotide for Capase-10 in Various Cell Types

The process of gene targeting via Chimeraplasty is achieved by using a RNA-DNA oligonucleotide homologous to a gene of interest ,to introduce a single base pair or frameshift mutation in genomic DNA .The extensive use of chimeraplasty is currently limited by wide variation in its gene conversion rates(.01-40%) and its mechanism of action remains to be fully understood. For cell studies, chimeraplasty is an alternative strategy to homeologus recombination in generating gene knockout models.

聚苯胺導電高分子二次電池之研製及性能研究

塑膠一向被認為絕緣體。但Heeger,MacDiarmid,以及Shirakawa證實,塑膠可以被改製成電的良導體。這須在高分子合成的過程中,使碳鏈呈現單、雙鍵交錯排列情形。此外,高分子也須加以摻雜(doped),換言之,電子必須在氧化過程中被移除或在還原過程中被加進聚合物。電子移除所留下的電洞,或新加進的電子則可自由在分子鏈上移動,形成導電性。我們所要製備的導電高分子二次電池就是由這個原理完成的。所謂的二次電池(Secondary Cell)簡單的定義就是「可反覆充電、放電,循環使用的電池」,我們將會將其與市售電池比較,並改良出合適商品化的高分子二次電池。我們將以聚苯胺做為我們電池的正極,並用鋅片(原本我們要用鋰金屬的,但其再空氣中即易氧化,因此改採鋅)做為負極,製成一聚苯胺二次電池。The plastics is thought to be a insulator, but Heeger, MacDiarmid, and Shirakawa had proved that conductivity of plastics can be improved by doping other media. The conjugated polymers such as polyacetylene、polyaniline have the interlaced single-double bonds that electrons can move from one side to another side caused the production of electric currents. The purpose of this study is try to study the application of the conjugated polymer polyaniline to make a secondary battery. First, we use the chemical and electrochemical method to compose the polyaniline. Then we test the conductivity of the produced polyaniline and test the current and electrical potential of the polymer battery. The battery that we made from our laboratory has the electric potential about 0.5 volts and the electric current is greater than 50 microamperes. Finally, we also try to use the lithium as cathode to improve the performance of the polyaniline battery.

聚球藻 RF 一 1 品系生物時鐘之特性

我們成功的用溶氣計偵測到了聚球藻 RF-1 在 28℃下光合作用的概日韻律。和傳統的研究方法比起來,這個方法具有連續偵測的優點,減少因不斷取樣所造成的影響,此實驗可觀察到聚球藻 RF-1 溶氣量之變化圖與一般藻類(如單殼縫藻)不同.在光 /暗條件下 RF-1 之溶氣量的增加與減少均呈週期性變化,而且此變化現象在進入連續照光後仍然可以維持兩個循環以上,這些結果顯示以溶氣計連續偵測聚球藻 RF -1之概日韻律是可行的,而且所得到的變化圖形遠比傳統方法(於不同時間取樣)所得者自然。本實驗同時發現含聚球藻之培養液的溫度,在進入黑暗週期時會有明顯的上升,由於其變化程度比其他藻類明顯,如加以探討應有助於對此藻以及其韻律特性之瞭解。We successfully detected the photosynthesis circadian rhythm of the prokaryote Synechococcus RF-1 under 28℃ by a DO (Dissolved-Oxygen) meter. The advantage of this method, comparing with the traditional methods, is that it can detect signals continuously, reduce the influence of discrete sampling. The DO curves of the Synechococcus RF-1 are different from that of other algae. Under Light/Dark conditions, the DO values of RF-i increased and decreased periodically. The periodic phenomena progressed over two cycles under constant lighting conditions. These results revealed the feasibility of using DO meter to continuous detect the circadian rhythm of the Synechococcus RF-1 The detected DO curves looked more natural than those obtained in the traditional discrete-sampling method. We also found that the temperature of the culture increased in dark cycle. Since the variation is clearer than that of other algae, further investigation will benefit the understanding of the Synechcoccus RF-i and its circadian rhythm.

The Solution to Global Water Pollution?

The waters of the Benguela, the Atlantic Ocean off South Western African shorelines, are amongst the most productive in the world, supporting prolific marine life. However despite the abundance of animals, survival in this marine ecosystem is not always easy. Oxygen-deficient bottom water, often containing toxic hydrogen sulphide, is a feature of the northern Benguela coastal upwelling system. Here, superfluous cells from excess phytoplankton production, decay and sink to the bottom to form the oozy diatomaceous mud belt sediment off the Namibian coastline. Within this diatomaceous mud further intensified decay takes place to form toxic hydrogen sulphide in the sediment. Sporadically large amounts of the hydrogen sulphide are released into the water column, causing the deadly annual "sulphur" events, as they are locally known in Namibia, to take place. Sulphur eruptions result in the deaths of thousands of marine animals. This marine system off the Namibian coast, with its harsh natural conditions of hydrogen sulphide and low oxygen, is similar to an ecosystem suffering intensive marine pollution. These natural conditions of the Benguelan waters are closely related to the conditions of many coastal areas suffering from the global marine pollution problem, created by man all over the world, through the discharge and disposal of wastes, such as nitrate fertilisers, sewage and biological decay material. A specialsed group of bacteria known as sulphur bacteria occur within the sulphidic conditions of the sediments. Sulphur bacteria actually use some of the produced toxic hydrogen sulphide in the sediments, converting it to harmless elemental sulphur micro granules in their cytoplasm. Where no other life occurs, due to the harsh conditions unfriendly to most marine lifr, sulphur bacteria thrive. Sulphur bacteria control and decrease the amount of hydrogen sulphide, which goes from the sediment into the water, through their complex metabolic systems. The biggest and most effective sulphur bacteria, only found off the Namibian coast, were dubbed Thiomargarita namibiensis. Thio means sulphur and namibiensis refers to its occurrence in Namibia. This giant sulphur-eating microbe is the earth's biggest known bacterium, visible to the naked eye. I conclude with a personal hypothesis to suggest a solution to global water pollution by utilising this magnificent bacterium. Through bacterial cultivation and processing Thiomargarita namibiensis could be employed in tacking extent of global marine pollution. The bacteria use toxic hydrogen sulphide as "fuel" for their metabolism and nitrate as an oxidizing agent, to produce harmless sulphur granules. This explains the bacteria's effectivity in removing nitrate and hydrogen sulphide wastes, the forms most biological wastes eventually occur in, from the water. This spectacular process, as it occurs within these magnificent "sulphur pearl strings", could be the sensational answer to the regeneration of polluted marine waters on a worldwide scale. This absolutely natural treatment of the water would not bear any harmful consequences such as those artificial treatment leaves behind. Sewage treatment or denitrifying treatments applied by man on polluted water leaves chemical discharge and damage to affected ecosystems. Especially Thiomargarita could be used in the regeneration of rural and urban waters (should those survive in fresh water)and sewage schemes as well as most marine waters, due to its high effectivity in removing hydrogen sulphide from affected water. It is not the solution to global water pollution to fight chemicals with chemicals. Nature has provided an excellent and valuable resource that could enable absolute natural recovery within polluted marine ecosystems. We should dedicate ourselves towards such magnificent discoveries and help nature help itself. Research on these Sulphur bacteria(especially Thiomargarita namibiensis)is very recent and ongoing. I recently enjoyed the privilege of a 24 hour marine research ship cruise off the Namibian coast with marine biologists from the Namibian Ministry for Fisheries and Marine Resources, to obtain mud cores holding Thiomargarita namibiensis bacteria. Several chemical tests are done and biological reactions are studied to collect the necessary data concerning Thiomargarita namibiensis. The discovery of Thiomargarita namibiensis awoke worldwide scientific excitement and interest. Its application to solve the problem of global water pollution would be a spectacular scientific breakthrough for the human race.

空間震盪與擴散

筆者將K?Cr?O?置於明膠溶液中煮沸,加入AgNO?水溶液,使之造成空間震盪反應。產生Liesegang ring。並且在研究空間震盪的過程中,筆者在研究過程中同時發展出一個程式,可以完成空間震盪實驗完成之後,由其震盪條紋的時空關係,得知ksp與擴散係數。或者可以在已知ksp和擴散係數的條件之下,預測出空間震盪實驗完成時的情形。We put K?Cr?O? into glutin, boiling it. After it concretizes, add AgNO? aqua into the glutin of K?Cr?O?. It is made to be space oscillation reaction with the Liesegang rings. And when we research for the process of space oscillation, we develop a program which can know the Ksp or diffusing coefficient after finising an experiment in Lablratory. Or we can predict the statement after the experiment on the condition of the Ksp and diffusing coefficient.

遞迥數列及渾沌現象

給定一個P∈(0,1),令k0=0, p0=p,定義k1為能使 的最小正整數k,而 ; 相同的,對於給定的kn-1, kn 為能使的最小正整數k, 。若存在kn 使得,則稱p∈ In; 若對於所有n 與kn ,,則稱p∈ I∞。如此區間(0,1)可分解成集合I1,I2,…,I∞。

Automatic Sterilization System for Operating Rooms?

The ASSOR is a device that allow to perform the sterilization function in hospital areas, the most important sectors of the hospital where it is required to implement this type of systems are the operating rooms, intensive cares, baby care, pathology, etc. The principle of the present project is a system that enables or either disables a valve that allows the fluid of the product, antiseptic or sterilizing, inside the room to be sterilized. The main application of this system is, after the manual cleansing of the room, in a control board, the procedure is turned on. The ASSOR consists, in general, of an electro valve that controls the fluid of the antiseptic into the room, a fan system, the electronic control circuit for the application of the product and electronic circuit to have a synchronized control of the system, and finally the product application in the target room.

台海的冬季訪客-鯔魚洄游的研究

每年一到冬季,常常會看到新聞報導有關烏魚洄游的消息。往往在當季讓漁民有可觀的收穫。恰巧國三的地球科學課時,第四章「地球上的水」有一小段提到:「冬季時台灣海峽由於東北季風吹送,中國沿海的海流向南流至澎湖群島附近,使台灣西南海域的水溫降低,正適合烏魚習性,因此每年在冬至前後左右,大量烏魚為台灣的漁民帶來可觀的魚獲。」為什麼冬季時台灣海峽會因東北季風吹送,中國沿海的海流會南流至澎湖群島附近?台灣酉南海域水溫降低,範圍有多廣?台灣的東南海域呢?烏魚有何習性?適合的海域水溫為何?除了水溫使大量烏魚洄游至台灣西南海域外,是否還有其他的因素?.....經過老師的鼓勵和指導後,我決定把烏魚洄游的情形整理出來並試著找出疑點的答案。

強電場下DNA穿透細胞膜的蒙地卡羅電腦模擬

去年暑假,我們從Newton雜誌得知,布洛貝爾博士(Dr. Gunter Blobel)由於發現了蛋白質如何在細胞之間運送,增進了人們對遺傳性疾病的了解,因而榮獲1999年的諾貝爾醫學獎。同為高分子鏈的DNA,在基因遺傳工程中常被用來在強電場的情況下穿破細胞膜,以進行基因的改造,其穿的物理機制又為何?在一場演講中,我們知道鍵振盪模型(bond-fluctuation model)可用來模擬高分子系統,因此我們想以之模擬DNA在強電場下穿透細胞膜的行為。