2025年

本研究主旨是在探討颱風與季風互動對颱風風場不對稱性變化的影響，分析了2013至2024年9月期間的颱風數據，結果顯示，季風是影響颱風風場形狀的關鍵因素。在東亞特有的季風氣候中，84%的颱風受到季風共伴的影響，我們發現，在季風共伴下，颱風的七級風場會呈現螺旋形，東北季風影響下多呈「6」形，西南季風影響下多呈「9」形，這些形狀可用「等角螺線」來描述，對於季風影響不明顯的颱風，風場形狀則更接近橢圓。我們進一步計算集合重合率以驗證形狀描述的準確性。 此外，本研究將颱風生活史的流型演變分為五類，結果顯示，環境條件相似的颱風，在流型變化上具有相似性。我們還利用颱風氣流場裝置模擬颱風風場，測量風速和風向，深入探討環境風場對颱風不對稱性的影響。

本研究開發基於RNN和LSTM的獨居者居家行為監控系統，利用樹莓派、多種無侵入式感測器及鏡頭實現獨居者日常活動的實時監控與情緒評估。系統收集各感應器數據，透過 LSTM 進行學習與預測，並使用隨機森林演算法分析行為狀態，提供客製化數據處理與預測性能優化。 長期數據收集可觀察獨居者的活動力及情緒變化，早期發現健康風險，針對現有求救設備頻繁異常通知的問題，我們開發了能夠識別長期身心狀況變化的監測系統，並整合人體紅外線感應器、都卜勒雷達感應器、日夜感測器等技術，透過Python程式及MQTT進行數據收集與處理。模型訓練使用SQLite資料庫數據，經過多次測試選擇最佳參數以提高預測準確度，隨機 森林演算法也強化決策準確性。 通過長期的數據收集和分析，本系統能夠觀察並預測獨居者的活動力和情緒的變化，早期發現獨居者的身心健康是否有風險。

克雷伯氏肺炎菌（Klebsiella pneumoniae）近年來於亞洲盛行，其除了傷害健康外，亦對於醫療經濟造成一定程度的影響。隨著時間發展，此細菌也逐漸獲得了抗藥性，使早期使用之抗生素不再有效，因此，尋找治療此疾病的替代療法成為近年來持續被關注的議題。由於其對細菌具高度專一性，噬菌體之莢膜多醣分解酶被認為極具開發抗生素替代療法之潛力。 本研究陸續於污水中分離純化出1212P2、H-P7、H-P8、0505P5、0505P6、05052P5、0505Kp3等噬菌體，並藉由一系列實驗，篩選出可能具有莢膜多醣分解酶的噬菌體。最後將候選噬菌體1212P2、0505Kp3進行全基因定序。 未來，將進行全基因序列分析，若成功比對並找到具有莢膜多醣分解酶潛力之基因，我們將對其加以表現並測試活性。並進一步進行體內試驗檢驗此噬菌體或其莢膜多醣分解酶的效力，探討其是否能作為開發藥物的工具，為對抗克雷伯氏肺炎菌盡一份努力。

利用鏡檢大生熊蟲形態檢測蔬菜中硝酸鹽壓力，常有形態判別問題，本研究想利用其自體螢光開發新型檢測模式，利用硝酸鹽壓力下其活動與隱生比例差異與自體螢光強度關係，檢測硝酸鹽濃度。顯示其自體螢光最佳激發波長為488 nm，製作檢量線(R2=0.99)與自製裝置使用470nm波長激發以壓克力濾光(R2=0.97)可檢測0〜156 mg/L硝酸鹽，可改善鏡檢缺點，並嘗試應用，發現蔬菜硝酸鹽 (小白菜492mg/L)，超出其自體螢光檢測極限，且蔬菜萃取液會影響大生熊蟲自體螢光，目前能進行定性分析，後續將分析蔬菜中造成干擾物質，繼續評估其應用性。探討其螢光機制，利用組織切片，探討大生熊蟲自體螢光強度與表皮層厚度在隱生和活動狀態下，是否具有相關性，發現脫水樣本自體螢光強度與螢光面積較活動樣本無差異(p>0.05)，推測自體螢光強度會受到其隱生時體表收縮程度有關。

抗癌藥物的研究一直受到重視，吲哚(indole)衍生物可助抵擋自由基，而二吲哚(Di-indole)衍生物已成為抗癌劑。鈣離子/鈣調蛋白依賴性蛋白激酶 (Ca2+/calmodulin-dependent protein kinase II，CaMKII)之抑制劑為癌症標靶藥物重要研究方向之一，抑制CaMKII可降低各種癌細胞增殖和存活，但目前尚無CaMKII抑制劑藥物。本研究以三吲哚為主架構，發展衍生物作為CaMKII抑制劑，期望可應用於抗癌劑。電腦軟體Discovery Studio2016模擬各種三吲哚衍生物分子模型與CaMKII α(PDB: 2VZ6)之結合能，選出結合能較大之化合物3，並延伸結構/活性(SAR)最佳化，進行一系列高效率藥物合成純化工作。經由送測生物細胞活性，其中先導化合物(lead compound) 3-1對癌細胞之毒性高且對CaMKIIα的抑制效果佳，符合癌症治療上的需求，將繼續最佳化此結構，並進行細胞訊號傳送途徑及動物實驗。

微塑膠在自然環境中普遍存在，甚至被生物攝取後進入食物鏈間傳遞。由於水蚤族群敏感性高，常作為監測水域環境變化的重要指標。我們以不同PVC濃度（0.01mg/L及0.1mg/L）為變因，探討微塑膠對於水蚤的生理、生殖及晝夜垂直遷徒(DVM)行為影響。結果發現0.01 mg/L、0.1 mg/L PVC濃度環境，皆不會影響蚤狀溞的存活率，但是會提高蚤狀溞的心率及降低趨光行為。而0.1 mg/L PVC濃度環境會使蚤狀溞提前進入生殖階段，子代數量明顯高於對照組，出生在0.01 mg/L 、0.1 mg/L PVC濃度環境的初生蚤體長有離散程度大的趨勢。蚤狀溞DVM屬於日間在深層、夜間在表層的Nocturnal migration 模式，但在具有PVC的環境中，夜晚有較高比例停留在深層，此一行為改變，也會反應在食物鏈後端掠食者上，最終甚至可能影響魚獲，若在水面增設燈光照明可以改善蚤狀溞夜晚停留在深層的現象。

Climate changes that include ocean acidification and global warming are serious problems in the ecosystem, affecting marine phytoplankton, including Nannochloropsis oceanica. In the effort to further explore the impact of rising temperature and carbon dioxide (CO₂) concentrations on oceanic ecosystems, the phytoplankton Nannochloropsis oceanica was used as a model organism. This study explored the effect of temperature change and CO₂ concentration on the growth of Nannochloropsis oceanica, achieving 243 samples that were tested with three different temperatures (24 degrees Celsius (°C), 28°C, 32°C) and CO₂ concentrations (0 milliliter (ml)/min, 0.4 ml/min, 0.6 ml/min), utilizing a hemocytometer counting method. Results indicate that the CO₂ concentration has a significant effect on the population of Nannochloropsis oceanica. But the temperature doesn't affect a lot. The Nannochloropsis oceanica in the lowest temperature and highest concentration of CO₂ in its environment had the highest population growth, and in the highest temperature and lowest concentration of CO₂, it had the lowest population growth. Results show the serious negative effect of climate change on the cosystem and the importance of environmental protection. Population blooms due to excess CO₂ taking up ocean resources causing dangerous ecological imbalances.

We invented the Midori which means green Bioreactor (MBR), beads of euglena and other microalgae fixed in calcium alginate that absorbs carbon dioxide (CO2). We examined the effect of 19 different solutions and two different organisms on MBR cultivation. Surprisingly, when the MBR was supplied with carbon dioxide or cultured with yeast, they became drastically darker green. Chromatography revealed this green color to be that of microalgae such as green algae or Euglena because chlorophyll a and chlorophyll b were detected. Under sunlight, MBR absorbed CO2 and the absorption rate was 1.5 L CO2/day/1L of MBR. Furthermore, when we put MBR in the water tank, they increased the amount of dissolved oxygen without polluting the environment. These results indicate that MBR can absorb CO2 by photosynthesizing without leaking out the inside microalgae.

本研究設計與合成一系列的電子供體分子，以研究分子單體的化學結構對於所形成的激發複合體光物理性質的影響。 五個所設計的供體分子已被成功的合成並確定均具有分子內電子轉移的性質 其躍遷偶級距變化分布範圍在17.6-28.6D之間。 將此五個供體分子分別與兩種電子受體分子在溶液聚集在一起，利用在長波長處所新生的螢光發光，推測激發複合體的形成。研究的成果並顯示，具有類似三角形結構的供體分子將更容易形成激發複合體，而具有棒狀結構的分子則較不易形成之。此成果有效的提供有關於單體分子結構的設計對於所需激發複合體光物理性質的影響，形成可快速地提供各式不同發光波長的材料，將可作為在發光二極體發光層材料、螢光感測器、生物成像等領域需求時的分子設計藍圖與指引。

According to the 印尼n Association of Olefin Aromatic and Plastic Industries/INAPLAS, 2019 national plastic consumption still relies on plastic packaging at 65% and surprisingly, around 60% of plastic waste is absorbed by the food and beverage industry. The waste has been widely sought to be environmentally friendly, one of which is by developing biodegradable packaging. The purpose of this research is to make durian peel cellulose nanocomposites impregnated with TiO2 NPs, to form antibacterial properties against E. coli wild type and resistance. In this research, there are research methods consisting of nanocomposite synthesis, PSA test, FTIR, physical characteristics test and resistance test. The results analyzed that the nanocomposite nanocellulose-TiO2 NPs was successfully made using a 1:1 ratio and had a particle size of 458.7 nm based on the PSA test, which is classified as a nano size. The success of nanocomposite synthesis was proven by the results of FTIR analysis, which showed the formation of 698.65cm-1 and 1633.99cm-1 spectra, indicating the peak of TiO2 NPs and O-H functional groups on TiO2 NPs, as well as 1028.98cm-1 and 1158.42cm-1 showing C-O and C-O-C bonds in cellulose. The antibacterial test performed showed no significant activity in disc diffusion and well diffusion tests against E. coli wild type and resistance. This is potentially caused by inhomogeneous particle size variation. Physical characteristics test showed that the tensile strength test (0.075 > 0.0125 MPa) Durio Nano-Pack is superior to styrofoam, but the compressive strength test (0.125 > 0.875 MPa) shows the opposite. In this study, nanocomposite has a potential innovation that provides good mechanical properties and has a dual function mechanically as bio-based food packaging and chemically as antibacterial. Further research is needed to improve the particle size homogeneity of nanocomposites, modify the impregnation method, so that it has the potential to develop multifunctional materials that excel in various applications.