2025年

An exploration of Pachyrhizus erosus as an option for plant-based milk in the 印尼n market for people with lactose intolerance. With its prevalence in tropical climates, Pachyrhizus erosus is an affordable crop in 印尼. Pachyrhizus erosus is a root vegetable containing a calcium content of 15.6 mg per cup (130g) in its unprocessed form, concentrated in its white flesh. Based on its affordability and calcium content, Pachyrhizus erosus can be transformed into a beverage product with nutritional qualities on par with existing plant-based milk, therefore being a solution for calcium sustenance that is more accessible due to its high capability to be locally grown in 印尼. However, this may be a partial case as Pachyrhizus erosus only thrives in regions with long warm seasons. In this research, three trials of Pachyrhizus erosus-based milk recipe have been conducted Trial 1 consists of a 1:1 ratio of Pachyrhizus erosus to water, Trial 2 consists of a 2:1 ratio of Pachyrhizus erosus to water, and Trial 3 consisting of a 10:10:1 ratio of Pachyrhizus erosus to water, and to a small amount of soybean. Based on the results of 14 organoleptic test respondents, it is concluded that the best ratio of ingredients is 10:10:1 (water: Pachyrhizus erosus: soybean) due to an overall preference of the third trial with this ratio, in terms of taste, aroma, color, and consistency. Pachyrhizus erosus is the dominant ingredient in developing alternate plant-based milk. However, findings from the most preferred trial in the organoleptic test suggest that implementing a minor amount of soybean would stabilize the milk-like consistency and flavor. Pachyrhizus erosus’ ability to retain calcium in its water content has been additionally proven in a calcium test using a reagent solution of ammonium oxalate, as even with the trials’ step of straining the liquid content of Pachyrhizus erosus that had been blended with added water, all three trials tested positive based on the high level of the precipitate. Other tests that tackle the quality of each trial include In Silico Testing, biuret protein test, alcohol test, COB test, and pH level testing.

Problem • Rice is the primary cereal crop consumed by nearly half the population worldwide • By 2050, there will be a 50% increase in demand for rice • The world’s poor populations depend more on rice, both for income and consumption, than any other food. Rice is the single-largest source of employment and income for rural people • Worldwide, 51–82% of agricultural crop yield is lost annually due to abiotic stress due to climate change • Climate change causes extreme temperatures, erratic rainfall, dangerous droughts, and increased salinity from rising sea levels Solution • To adapt to abiotic stress, rice has intricate signaling pathways, particularly those mediated by the phytohormone abscisic acid (ABA), that cause an increase in stress tolerance • Clade A genes of the Protein Phosphatase 2C (PP2C) gene family are known to be negative regulators of the ABA signaling pathway. • “Deleting” these genes activates the ABA pathway and increases stress tolerance in rice without inducing stress CRISPR gene editing technology is the ideal solution Research Goal • While the role of PP2C genes in stress response is recognized, there is a gap in understanding the specific genes within this family that contribute significantly to stress signaling. Furthermore, there is a need for a detailed investigation into the effects of targeted CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) genome editing on rice stress response pathways.

Cultivating plants from seeds or cuttings is a fundamental aspect of gardening and agriculture. While traditional methods have been practiced for centuries, there is a persistent need for innovative and efficient approaches to enhance plant growth and development. This section explores the challenges associated with traditional propagation methods and examines potential solutions offered by emerging technologies and materials. Plant propagation is necessary to allow efficient multiplication and distribution of desirable plant varieties (Sorensen & Garland, 2024). Plant propagation is the process of creating new plants. There are two primary methods of propagation: sexual and asexual. ．Sexual propagation involves the union of pollen and egg, drawing genetic material from two parent plants to create a new, genetically diverse offspring. This process utilizes the floral parts of a plant. ．Asexual propagation, on the other hand, involves taking a part of a single parent plant and inducing it to regenerate into a new plant. The resulting offspring is genetically identical to its parent. This method utilises the vegetative parts of a plant, such as stems, roots, or leaves. One emerging technology that has garnered attention in this field is the use of cocopeat, a sustainable growing medium derived from coconut husks (Pane et al. 2021). Cocopeat has been extensively studied as a potential alternative to peat moss in plant propagation (Gericke, 1940). It offers a favourable balance between air porosity and water holding capacity, promoting root development and nutrient uptake (Kalaivani and Jawaharlal, 2019). Furthermore, cocopeat is a renewable and environmentally-friendly resource, making it an attractive option for sustainable seedling cultivation. Research has shown that the use of cocopeat as a growing medium can enhance the growth of both vegetables and various ornamental plants, such as Impatiens. The biostimulant effect of the Trichoderma atroviride fungus, which can readily colonize coir, has been observed to increase aboveground biomass, flower production, pigments, and nutrient concentration in these plants (Traversari et al., 2024).