全國中小學科展

2024年

老化相關之組蛋白N端乙醯酶Naa40p於小鼠神經細胞HT-22的功能探討

表觀遺傳學(Epigenetics)是研究行為與環境因子如何對基因表現產生影響, 主要是藉由DNA甲基化、組蛋白修飾、非編碼核醣核酸來影響基因表現, Naa40p (N terminal-alpha-acetyltransferase 40 protein) 也稱為NatD、Patt1或Nat4 是一種組蛋白乙醯轉移酶,修飾組蛋白H2A及H4。在酵母菌的研究中,Naa40p 的缺失可以延長酵母菌的複製壽命(Molina-Serrano D., 2016)。在The Human Protein Altas資料中,可以看出人體中Naa40p在腦部有較高的表現。因此想了解Naa40p在小鼠海馬體神經細胞HT-22中具有什麼樣的功能。 藉由CRISPR基因編輯技術產生Naa40p剔除的突變株,進行細胞功能的檢測,在移動能力相關的傷口癒合測試中,Naa40p剔除的突變株癒合速度明顯快於野生型,期望能藉由更多細胞功能的檢測來更全面地了解Naa40p於神經細胞相關的功能與機制。

排排相扣—2341和3421 – avoiding 交替排列的組合關係探討

abcd–avoiding交替排列中的任⼀偶數項都要⼤於相鄰之奇數項,且其中任意四項皆不能有「abcd」的大小關係(「abcd」為 1 ~ 4 的⼀種排序),⽽偶數⾧度的 2341 和 3421–avoiding 交替排列皆為三維卡特蘭數的組合表徵。 本研究欲探討這兩種交替排列的組合關係以及可能的互相變換⽅法,我們發現兩種排列中「數字 1 在各項出現次數」有相同的分佈。我們推測可以透過移動數字 1 的位置在兩種排列中分別建⽴不同排列之間的對應的關係,並找到了兩種排列中部分的「數字 1 在第(2𝑘 − 1) 項」排列和全部的「數字 1 在第 (2𝑘 + 1) 項」排列互相變換的⽅法。利用這種排列關係,我們還證明了「數字 1 在第 (2𝑛 − 1) 項」的 2341 和 3421 – avoiding 交替排列具有一一對應的雙射變換法。

修正未切換注音輸入法產生之字元

臺灣的許多人要使用電腦打字時皆會選擇「注音輸入法」作為輸入的方法。然而要使用注音輸入法輸入中文時若無切換輸入法則有可能會誤用到英文輸入法。如要輸入「今天天氣好」五字,使用英文輸入法輸入時會輸出「rup wu0 wu0 fu4cl3」。這種文字不易理解。本研究的目的即為研究將未切換到注音輸入法而打出的英數符號混和字元翻譯為漢字的方法。 本研究使用「PTT 中文語料」與「維基百科中文資料庫」訓練 GRU、BiGRU、LSTM 和 Transformer 以及計算維特比演算法,並與 Google 輸入工具的進行比較。整體來看以 PTT 中文語料計算的維特比演算法的 BLEU4 分數最高,在準確率以及 BLEU4 的評分皆高於Google 輸入工具,分數分別為 0.94 與 88.3 分。 本研究之成果在應用方面極廣,可應用於線上翻譯或聊天軟體的即時翻譯。本研究使用之程式碼開源於 GitHub 頁面,除了可讓使用者下載使用外,使用者也可訓練自己的模型。

Look your eyes,know your life~A portable body detection device

本研究是基於去年的 PBC(Protect Brain Cylinder)作品[1],並根據評審指導將作品做了大幅度修改。相較於 PBC 需要一個額外的裝置,本研究僅須在手機相機前方,安裝光源處理模組,搭配本團隊開發的 App,即可清楚地拍攝出瞳孔影像。並經由 App 中的影像分析功能,即可在影像拍攝完成後的兩秒內完成分析, 並將結果繪圖呈現在手機螢幕上。偵測的時間相較 PBC,大幅縮短了 60%。 相較 PBC 僅能偵測患者瞳孔直徑對光刺激的反應速度。本研究除了大幅縮短偵測的時間外,在結果分析及應用上更有長足的進步,能判斷出以下的五種身體狀況: (1)瞳孔形狀 (2)虹膜脂質堆積環 (3)鞏膜血絲分布及比例 (4)水晶體混濁與白內障關係 (5)瞳孔直徑對光刺激的反應速度與酒測值的關係 本研究除了藉由偵測瞳孔來判定身體狀況外,更開發出了身體『傷口大小量化』功能。希望協助醫護人員一機在手,即可解決上列的臨床問題。

圓桌中對應編號的錯排問題

本研究主要探討,有n位教授要在一個圓桌上舉行會議,其中每位教授都有自己的編號 (1~ n號),同時圓桌的 n個位置上也有各自的名牌編號 (1 ~n 號) 以順時針擺放置圓桌上與教授們的編號對應。其中第一個進來的 1號教授坐到了圓桌上 k號位,此後的教授們亂序一個一個進入,若發現與自己編號相同的位置是空的,就直接入座;若與自己編號相同的位置被占走了,就以逆時針方向尋找空位,直到有空入座。在這樣的遊戲規則下,本研究探討了,有 n位教授,且 1號教授坐到 k號位,如何給定一組教授入場的順序,就能即刻的找出對應的坐法,以及計算坐錯人數的期望值和坐錯人數次數分佈表等等,後續再將遊戲規則改為,1號教授不限定為第一個入場的人,同樣的探討上述問題。

Sport specific assessment of inter-limb asymmetries: A way to reduce injuries

In recent years, lower inter-limb asymmetries have become a topic of increasing interest in sports research. Numerous studies have investigated the occurrence of between-limb differences in a variety of physical tests, ranging from strength, sprinting, and change of direction speed to jumping tasks. The main focus has been the association of asymmetries to either enhanced injury risk or reduced physical performance. Sport specific aspects and differences of lower limb asymmetries have not often been analysed. Additionally, most studies have been performed with male athletes. Women, however, present higher prevalence of lower limb asymmetries in strength, coordination, and postural control than men. These two aspects were therefore addressed in the present study. Female youth soccer (n=18, age: 16.7 ± 0.8 years) and floorball (n=18, age: 17.6 ± 0.9 years) players completed a test battery consisting of six unilateral jumping tasks in horizontal and lateral direction to detect sport specificity in inter-limb asymmetries. The test comprised the following hops: (1) Single Leg Hop for Distance and (2) a newly created version of it, (3) Single Leg Triple Hop, (4) Single Leg Crossover Hop, (5) Side Hop and (6) Single Leg 6-meters Timed Hop. The scores of every jump were calculated into Limb Symmetry Indexes for each participant. A linear mixed effect (LME) model (using function lmer in program R) was applied to evaluate the effects of sport and jump type on asymmetries. In terms of the whole test battery, there was a significantly higher magnitude of asymmetries in soccer compared to floorball (p=0.0067) with a mean difference of 1.9%. Three significant differences between the effects of different jump types were detected (5>2: p=0.027; 5>3: p6: p=0.014). Moreover, the results showed no significant effect on leg dominance. According to the findings of this study, soccer appears to be more asymmetric than floorball, leading to the suggestion that inter-limb asymmetries may not only be task-specific, but also sportspecific. Since no significant correlations were detected, this study suggests that inter-limb asymmetries are independent of leg dominance. Considering the possible reduction in athletic performance and increasing injury risk, strength and conditioning coaches are advised to assess athletes' inter-limb asymmetries using a broad, sport specific test battery and decrease them.

In silico Screening of Forty Antiviral Phytochemicals as Inhibitors to the Envelope Protein of Dengue Virus Serotype 2 (DENV-2)

Infections by the Dengue virus (DENV) cause a disease amonghumansreferred to as Dengue fever, which causes thousands of fatalities globally. There is no existing treatment as of yet that successfully targets DENV. Among the factors thatdeterminetheentry of the virus and severity of the disease is the envelope(E) protein of DENV. This study aimed to examine forty antiviral phytochemicals enumeratedinpaststudiesaspossibleinhibitorstotheEprotein of DENV to provide candidates to aid in drug discovery against DENV. The phytochemicals were screened for their likelihood of inhibition of the E protein using AutoDock Suite and LigPlot+. Seven phytochemicals produced favorable binding affinities to the E protein, which are based on the interactions between the phytochemicals and amino acidsintheactivesiteoftheEprotein.Lipinski’s rule of 5 was then used to screen the seven phytochemicals for oral bioavailability. Glabridin has a binding affinity of -7.6 kcal/mol and was predicted to be orally bioavailable. This phytochemical interacts with amino acids in the E protein active site through hydrogen bonds to Asn355, andPhe337, as well as ten hydrophobic interactions. These interactions ensure that glabridin is able to specifically target and fit intotheactivesiteoftheEprotein, preventing its binding to the host cell and activating its viral proliferation. Glabridin is known to be found in the roots of licorice plants, providing anatural source for a possible cure for Dengue fever.

3D Arithmetic Billiards investigating edge points with a number theoretic approach

The billiard table is a cuboid with integer side lengths. A point-wise ball moves with constant speed along segments making a 45◦ angle with the sides and bounces on these. We allow the ball to start from any of the 8 corners, resulting in a periodic trajectory known as a corner path. The geometry of the path depends on the artihmetic properties of the side lengths (for example if these are pairwise coprime). Points of contact between the ball and edges, known as edge points, are inves- tigated and their characteristics like distribution explicitly described. This generalizes a previous work by Perucca, Reguengo da Sousa and Tronto of University of Luxembourg.

探討長片段非編碼RNA:IRX4-AS1在前列腺癌中的角色 Investigate the Role of Long Non-Coding RNA: IRX4-AS1 in Prostate Cancer

前列腺癌(PCa)是全球男性最常見的惡性腫瘤,雄性激素受體拮抗劑是常用的治療藥物,而其存在抗藥性演進問題。人類基因組有98%的基因未編碼蛋白質,許多研究證明這些非編 碼RNA在細胞中具重要功能,並與前列腺癌進程有關。 本研究藉由分析病人檢體資料,發現IRX4-AS1在前列腺癌細胞的表現量高於正常細胞,並且IRX4-AS1表現量較高的病人五年存活率較差。我們也在體外實驗發現,IRX4-AS1的202異構型主要表現在細胞核;203異構型則多在細胞質,兩者可能有不同作用機轉。相較於IRX 4,IRX4-AS1的穩定性較高,顯示其可能與蛋白質共同作用。在抗藥性細胞株中,IRX4-AS1表現量則低於正常細胞株。 未來將研究IRX4–AS1對前列腺癌惡化與抗藥性的影響,期待其能做為疾病診斷及預後的生物標誌物。

探討C1GALT1與IL-1受體醣基化在關節炎中的角色並尋找可能的治療藥物

C1GALT1為氧型醣基化的關鍵基因,若以 IL-1β與 TGF-β刺激 ATDC5細胞株模擬關節炎,C1GALT1 的mRNA 與蛋白質表現量增加,而這樣的趨勢在誘導關節炎的小鼠軟骨組織中也可以觀察到。抑制 C1GALT1 的mRNA 後,促炎基因-一氧化氮合酶(iNOS)與第二型膠原蛋白基因(Collagen II)的 mRNA 表現量下降,代表 C1GALT1 參與了促進發炎的調控路徑。透過以大數據為基礎的 NetOGlyc-4.0平台分析,我們發現小鼠和人類的 IL-1β受體上都含有可能的氧型醣基化位點,C1GALT1 可能透過醣基化 IL-1 受體以增加 iNOS 的表現,進而促進發炎。抑制 C1GALT1的mRNA會使 IL-1R1的蛋白質表現量下降,而抑制 C1GALT1的mRNA或是蛋白質皆可使 IL-1R1在核周內膜系統中的表現量減少,代表 C1GALT1所促進的醣基化修飾是調控 IL-1R1形成的關鍵,抑制 C1GALT1可能具有減緩關節炎的療效。伊曲康挫(Itraconazole)可抑制 C1GALT1 且對人體傷害極小,透過老藥新用,或許能夠應用於治療關節炎。