In silico identification and physicochemical analysis of potential novel antimicrobial peptides from Momordica charantia L.
The emergence of antibacterial resistance has necessitated the development of alternative treatments, such as antimicrobial peptides (AMPs). AMPs are part of the innate immune systems of various organisms such as Momordica charantia L., a known medicinal plant in Southeast Asia. In this study, potential novel AMPs from M. charantia were derived in silico to provide prospective antibiotic alternatives using promising plant-based peptides. M. charantia protein sequences that were 500 amino acids long were digested using proteolytic enzymes, resulting in 3,621 peptides. Each resulting sequence was characterized as either AMP or Non-AMP using four statistical analysis tools, and those identified as AMPs were analyzed. This led to 102 AMPs, 53 of which were unregistered on the Data Repository for Antimicrobial Peptides, indicating that they have yet to be derived from other species. Six of the eight studied physicochemical properties show strong correlations with each other, suggesting that subsequent AMP design studies may focus on these six properties. As such, M. charantia may be a rich source of potential AMPs and, thereby, alternative antibiotics. The in vitro examination of these novel AMPs is also recommended to further understand their potential as alternative antibiotics sourced from locally available plants.
Two Klatovy copies of Šimon Brixi's Domine ad Ad juvandum me festina and his time
The main goal of this paper was a creation of musical score for the composition from the 18th century Domine ad Ad juvandum in A minor and C major composed by Šimon Brixi. Another goal was finding as much information as possible related to this composition and summarize everything possible that was written previously. Separate parts were transcribed into one partiture. Nevertheless there were difficulties like mistakes in sheet music (corrected according to harmony of other voices), missing beats (replaced by identical parts of score), differences in period station. Hard times also bring on differences of period notation. The resulting musical scores can be directly used for music production, which is schemed for the next year. The second part was researching new or never-published information related to these compositions. Here I describe Š. Brixi´s life. I managed to read out the cause of Šimon Brixi death in a chronicle, which was not in the publication I found. Brixi died of tuberculosis like his son. Transcriptors, J.V.Flaška and J.J.Hoffman, are also mentioned. Lastly the information about sheet music itself and its historical context were described. Another discovery was an identification of watermark on sheet music paper. Assuming the sheet music is truly transcribed in 1742 the Kolinec paper mill watermark on the sheet music paper is the oldest evidence of its use. In this paper I summarized the historical context of the baroque era including social life, art and culture.I mentioned the most important events that were crucial for historical development in Bohemia and especially in the Klatovy region. This context can be useful for today’s musicians who want to understand the historical background and express the composition with full respect to the time of its creation.