全國中小學科展

2023年

surgical masks and microplastics in our airways

The surgical mask has been our daily companion since the outbreak of the Corona pandemic. The nonwovens (outer layers, not the filter membrane) from which the surgical mask is constructed consist of very long and thin polypropylene fibers. This leads to the question of whether microplastics are released during breathing through the surgical mask, which could enter the respiratory tract or the lungs. This would have a negative impact on our health, depending on the size of the detached fiber fragments - the smaller the worse because they can enter much deeper in our respiratory tract. In order to investigate the question of whether fiber fragments are released during breathing through a surgical mask, a filtration device was built. The filters were examined under an optical microscope after filtration. If fiber fragments would detach from the surgical mask, they would be found on the filter. Different surgical masks were tested, those that were not worn at all to surgical masks that were worn all day. It was found that fiber fragments were coming off the surgical masks. There were different fiber fragment types. Some fiber fragments were still undamaged (exhibited nice fractures), while others were frayed. Clump-like fragments occurred, but also smaller fine fiber fragments. All these different fiber fragments had a certain size, so that they could be called microplastics. The remarkable result of the whole study is that there is a direct correlation between the wearing time of the surgical mask and the number of detaching fiber fragments. In the case of the unworn surgical masks, 10 times fewer fiber fragments occurred during filtration than in the case of the surgical masks that were worn all day.

Utilization of Escherichia coli in Contiminated Water in the Citarum River as a Dual Chamber Baed On Microbial Fuel Cell (MFC) Substrat

Citarum River is the longest and largest river in West Java. The upstream of the Citarum River starts from Mount Wayang, Bandung Regency and ends at the mouth of the Java Sea which is located in Muara Gembong, Bekasi Regency. The Citarum River plays an important role as raw water for PDAM drinking water, supplies electricity in Java-Bali and provides water for rice field irrigation in West Java. Citarum watershed is dominated by the manufacturing industry sector such as chemicals, textiles, leather, paper, pharmaceuticals, metals, food and beverage products, and others. Based on data from the World Bank, every day, the Citarum River is polluted by approximately 20,000 tons of waste and 340,000 tons of waste water with the majority of the waste contributors coming from 2,000 textile industries. By looking at these events, there is no doubt that the sustainability of the ecosystem and the environment in the Citarum River is damaged and polluted. (Zahra Fani Robyanti; 2020). The West Java Regional Environmental Management Agency stated that the content of E. coli bacteria in the Citarum River had increased. The bacteria that cause diarrhea come from industrial and household waste. In addition to E. coli bacteria, other pollutants in the Citarum River that have increased are biological oxygen demand (BOD), chemical oxygen demand (COD) and Suspended Solids. One of the efforts that can be done regarding E. coli bacteria that pollute the Citarum river is to make it as a substrate for Microbial Fuel Cell (MFC). Bacteria present in organic media convert organic matter into electrical energy. The nature of bacteria that can degrade organic media (enrichment media) in MFC produces electron and proton ions. It is these ions that produce an electric potential difference so that energy can be generated. Generally in conventional systems, MFC consists of two chambers consisting of anode and cathode chambers. The two spaces are separated by a membrane where proton exchange occurs. This system has not fully worked with bacteria because only the anode side contains bacteria, while on the cathode side it still works using chemical compounds such as Polyaluminum Chloride (PAC). However, recently MFC has been developed using bacteria at the cathode, or better known as biocathode. Bacteria in the cathode space have the same function as electron mediators that were previously carried out by chemical compounds. In many studies on MFCs, acetate is commonly used as a substrate for bacteria to generate electricity. These chemical compounds are easier for bacteria to process than wastewater. Acetate is a simple chemical compound that serves as a carbon source for bacteria. Another advantage of acetate is that this compound does not cause other reactions to bacteria such as fermentation and methanogenesis at room temperature. Based on this thought, the author will design a study entitled Utilization of Escherichia coli Bacteria in Contaminated Water in the Citarum River as a Dual Chamber Based Microbial Fuel Cell (MFC) Substrate.

探討不同型態的海底峽谷對底棲動物群聚結構的影響

深海複雜多變的環境因子塑造了多樣的生態棲地,海底峽谷便是其中之一。全球9000個海底峽谷涵蓋了大陸斜坡總面積的11.2%,其中有6個峽谷分佈在臺灣西南海域。本研究結合作者出海採得的樣本與國內海洋研究所的採樣資料,探究西南海域高屏與枋寮峽谷的生態結構。 本研究應用生態統計分析,發現兩峽谷的主要差異為沉積物來源、粒徑分佈與物理性擾動強度不同,使兩峽谷的生物密度隨深度變化趨勢截然相反,生物組成亦存在顯著差異。接著藉由建立模型分析,驗證環境擾動與食物量為形塑區域性生態結構的主導因素,結果發現環境擾動對生物的影響幅度大於食物量,並討論了峽谷地形作為天然實驗室的潛力及未來研究發展。

「噬」者生存—有絲分裂時,USP24的下降誘發細胞自噬,有利於基因體的穩定

抗藥性是癌症治療時的一大阻礙。如何解決癌症的抗藥問題是十分重要的議題。過去的研究指出在肺腺癌細胞株中USP24表現量在有絲分裂期間會明顯下降,但此現象之功能仍有待釐清。在本研究中,我們發現該現象會使TRAF6表現量下降,從而使Beclin-1表現量上升,進而誘發細胞自噬。另外,我們更發現了有絲分裂期的細胞自噬有助於減少DNA碎片,達到穩定基因體的功用。此外,在與抗藥細胞株比較時發現,上述誘發細胞自噬之現象在抗藥細胞株中明顯下降。因此,我們推斷在抗藥細胞株中有絲分裂期的USP24下降變少,細胞自噬被抑制,造成基因體不穩定,最後造成抗藥性的產生。綜上所述,我們的研究不只闡明了USP24的下降在有絲分裂期間是如何誘發細胞自噬,更說明了有絲分裂時的細胞自噬有助於基因體的穩定,從而在肺癌的治療中阻止癌症抗藥性的產生。

合成CaMKII抑制劑作為抗癌潛力藥物

鈣及攜鈣蛋白調節酶(CaMKII)存在於大部分細胞中,研究顯示其在多種癌細胞中過度表現。苯基磺醯胺衍生物為CaMKII抑制劑的常見結構,而吲哚與靛紅是常見藥物結構,因此本研究參考文獻[1],並擴展臨床尚未解決問題,優化此兩類衍生物以製成抗癌藥物。第一系列以苯基磺醯胺為主架構,改良文獻[1]中抑制效果最佳的化合物1,第二系列則修改吲哚與靛紅的取代基,設計出多種候選分子。初步利用分子模擬分析軟體(Discovery Studio)模擬蛋白質與藥物分子結合,考量結合能、結合方位、藥物取得及合成難易度,選定有潛力的WACY與CYWA系列結構進行合成。本實驗使用創新的步驟合成,簡易、高效率且符合綠色化學,再經核磁共振與高解析度質譜儀驗證得出高純度產物。最後根據生物檢測發現WACY-2對乳癌細胞毒性高和對於一般乳腺細胞和乳癌細胞的選擇性都較市售抑制劑KN-93佳,而WACY-6對CaMKII抑制效果為WACY系列最佳;CYWA-2, 3, 6, 7對乳癌細胞的毒性和對CaMKII抑制效果又優於KN-93與WACY系列許多。本研究成功製備出符合理論計算預期且抑制蛋白質的效果較KN-93佳的化合物,未來極具潛力作為抗乳癌的標靶藥物。

利用懸臂梁振動探討物體與黏滯流體交互作用力之關係

本實驗應用3D列印及機械機構設計簡諧運動機制,使懸臂梁(以鋁尺構成)與聚乙烯醇及水混合之黏性物質碰撞,以雷射位移計觀察和測量鋁尺振動波形的振幅,探討其週期振動。 實驗中,調整聚乙烯醇與水的混合比例改變其黏滯度,以單自由度運動方程式探討鋁尺於不同黏滯度下的運動行為,發現鋁尺黏滯時間、脫離黏膠的速度與聚乙烯醇的濃度呈正相關,代表可利用量測黏滯時間、脫離黏膠的速度推測物質的濃度。加速度因同時受時間、速度影響,不與聚乙烯醇濃度呈正相關。另外,以黏度計測量不同溫度、聚乙烯醇溶液濃度下的黏度,發現聚乙烯醇溶液在重量百分濃度15%至25%的區間中,濃度愈高、或溫度愈低的情況下,黏性愈強,且濃度高者隨溫度變化程度愈大。 此外,以快速傅立葉變換處理鋁尺在受不同黏滯度作用下的位移數據,發現位移波形為簡諧運動,其頻率由鋁尺共振的自然頻率組成,不因黏滯阻尼而改變。驅動以振動器試圖改變鋁尺振動頻率,發現鋁尺傾向振動的頻率為其自然頻率,符合以歐拉-伯努力方程式推導出的自然頻率。

不老化動物櫟葉指形軟珊瑚生成天然化合物解析

本研究針對臺灣恆春半島海域所採集的櫟葉指形軟珊瑚Sinularla querciformis進行天然物化學成分研究,由此珊瑚中分離出兩個新型菸草烷類型天然化合物,分別是Querciformolide G (1)與Querciformolide H (2),以及兩個已知菸草烷類型天然化合物,分別是Sinulaparvalide B (3)與3,4:8,11-Bisepoxy-7-hydroxycembra-15(17)-en-1,12-olide (4)。上述化合物的物理性質和化學結構皆是由核磁共振儀、紅外線光譜儀和質譜儀等數據,以及比對相關化合物的文獻來分析確認。 針對化合物1-4進行抗發炎測試,對超氧陰離子產生和人中性粒細胞彈性蛋白酶釋放的抑製作用,發現化合物2針對彈性蛋白酶,有顯著的抑制效果。

提升纜車遭遇陣風之安全性-利用自製調諧質量阻尼器與可調式吊臂

遭遇強風是纜車停駛的條件之一,然而我們認為當低於停駛標準的陣風與纜車產生共振時,更會使纜車產生擺動造成危險。因此我們參考真實纜車的比例,製作出模型探討不同頻率及速度陣風對纜車造成的影響。我們發現即使風速未達停駛標準,但當其頻率與纜車接近時,即會發生共振並產生將近 30度的擺角、強度相較持續風吹拂增加 60分貝。 為了減低振動,我們首先製作可調式吊臂。但因為其在實際製作上具有困難,且可能造成乘客的不適。為此我們製作了調諧質量阻尼器,當共振發生時,將砝碼透過伺服馬達放下,使纜車的振動傳導至垂下的擺減緩振動。結果顯示阻尼器能將振動減低 10分貝,最佳的組別甚至有 16分貝的減振效果,能將擺角減低至小於 2度。期望將來能將系統自動化,在纜車遭遇陣風時自動調變阻尼器,抑制振動。

全等三角形與四邊形構造的衍伸圖形之性質研究

本研究源於 2016 年數學雜誌 Crux Mathematicorum 的三角形定性問題[1],我們將這個問題由四個方向進行推廣——多邊形邊數、頂點連線方式、等腰三角形角度、全等多邊形的夾角,再創新探討其定量與定性性質。首先,我們將任意全等的三角形與任意四邊形夾角為任意實數下的衍伸圖形之定量與定性性質進行完整刻劃,利用測量師公式分別針對不同連線情形下的兩個衍伸圖形的有向面積之和與有向面積之差進行完整討論,再巧妙利用平移不變性處理行列式級數和而給出面積不變量關係式。第二,透過向量、矩陣運算與純幾何方式探討面積不變量的幾何意義,並給出衍伸圖形之間的全等、相似、透視、對稱、共線、平行、退化等優美的定性性質。最後,我們系統性推廣到平面上任意封閉多邊形,嘗試以較高的觀點切入,透過矩陣變換給出其幾何結構,並且發現許多實質有趣的定性與定量統一結果。

探討圓及橢圓上的格子點個數之連乘積表達式

在坐標平面上, 坐標均為整數的點稱為格子點,在這篇作品中,主要探討圓及橢圓x^2+sy^2=m上的格子點個數,並且此個數以連乘積表達式呈現,其中s為黑格納數,此時虛二次體Q[√(-s)]的整數環為唯一分解整環(簡稱UFD),由此性質可得到虛二次體的整數環中任一元素的分解有唯一表示法。 首先探討質數p在虛二次體Q[√(-s)]的整數環中的分解性,是根據分解性分成四類,再由四類決定m的不可約元分解,進一步推導出x+y√s i,x-y√s i可能的唯一表示法,再由唯一表示法來計數圓及橢圓上的格子點個數。