全國中小學科展

2022年

Susanito, autonomous robot body temperature meter: support to reduce infections in rows

A variant of the coronavirus (2019-nCoV or COVID-19) was reported in Wuhan, China on December 31, 2019, spreading rapidly around the world. Owing to a plan to reactivate the economy, the Mexican government, requested to implement protective measures to enter establishments with confined spaces: wear a mask, provide alcohol-based hand rub and the measurement of body temperature, allowing problem when not have a fever; however, these measures cause long waiting lines, causing contagion risk. To support this problem, applied and experimental research was used, generating as a result, the creation of a robot that moves autonomously thanks to a line follower sensor. Dispenses alcohol-based hand rub and determines the temperature by means of an infrared sensor, checking that the distance is adequate, with an ultrasonic sensor, using a buzzer that emits a sound of half a second when it performs a normal detection, but, if it is equal to or greater os 38 ° C, emits a sound for 10 seconds asking the person to leave the line and go to a medical service, helping to reduce problems infections in lines

The Population Structure of the Orange River mudfish (Labeo capensis) in Allemanskraal Dam and Its potential as a Fishery Species

The aim of this research was to investigate whether the ecology and biology of the Orange River mudfish Labeo capensis were suitable for the species to be used in fisheries. Three fleets of the gill nets were set, parallel to the shore. One fleet was lifted, and the fish were collected by hand. The two remaining fleets were lifted the next day. The seine net was pulled for 10 metres within the littoral zone. The net was then pulled towards the shore of the dam and the procedure was repeated four times. The four fyke nets were set parallel to the shore and were left for two nettings nights and then lifted. All fish caught were collected by hand and placed into buckets. The majority (82.93%) of the fish caught were within the 0-100 mm size class. The 101-200mm and 201-300mm size classes contain similar numbers of fish, while no fish were caught in the 301-400mm size class. The hypothesis was accepted. Allemanskraal Dam, as of the study period, has a very small juvenile fish population of L. capensis, as only 7 out of 41 fish individuals caught were within the 101- 300mm fork length size class. These results show that the population of L. capensis is not established as of yet, as the research did was right after their breeding season. Historical research has shown that sexually mature individuals of the L. capensis species tend to be a minimum of 300mm SL, 4-6 years after hatching. The population was largely young-of-the-year and may develop into an established population in 3-4 years (after sexual maturity). The L. capensis population in Allemanskraal Dam has the potential to be a fishery species if suitable conditions are maintained. Establishing this species’ potential will therefore allow economically viable fisheries to utilise them sustainably and to their full economic potential.

分子拓印修飾磁性奈米粒子萃取福壽螺卵中蝦紅素之探討

本研究以二氯化鐵和三氯化鐵所合成之磁性奈米粒子為基底,包覆上以蝦紅素為模板修飾的二氧化矽,製作出具有蝦紅素專一性的磁性奈米粒子,應用於萃取福壽螺卵中之蝦紅素。 利用干擾物證明磁性奈米萃取粒子具有蝦紅素的專一性,再探討奈米粒子合成及萃取條件的影響。福壽螺卵經過打碎離心後,依序加入0.2 M氫氧化鈉及1 mM十二烷基硫酸鈉,使蝦紅素水解並去脫去蛋白質轉換為游離態。當模板濃度為0.059 mg/mL、TEOS濃度為1.892 mg/mL和蝦紅素的濃度為0.07 mg/mL時,會有最佳的萃取率達60.7 %。此磁性奈米粒子在萃取步驟後,再以丙酮進行脫附,至少重複使用3次。此技術可減少福壽螺的農害,也極具經濟價值,很值得研究。

平流層極地渦旋及北極震盪與區域極端寒冷事件之關係

本研究使用NOAA NECP Reanalysis Data 2000年~2020年冬季之緯向風、重力位高度場及其距平變化,探討北極震盪指數(AOI)、極地渦旋對北半球區域極端寒冷事件之關係。AOI與對流層300hPa北緯50~65度緯向平均之緯向風速變動關係較顯著,而與平流層(50hPa)極地渦旋的緯向風速變動僅呈現中度正相關。在太平洋區中,極端寒冷事件發生在AO負相位的比例最高,不過,極端寒冷事件不必然僅發生在AO負相位的大氣條件下,而是與極區的重力位高度場變動有關,且可能發生延遲影響。當極區平流層(50hPa~100hPa)或極區對流層(300hPa~500hPa)的週平均重力位高度距平值明顯上升,代表極地渦旋發生變化,大多有伴隨有極端寒冷事件的出現,即使處於AO正相位的情況亦然,本文對於區域極端寒冷天氣事件之重力位高度場特徵做歸納與說明。

半正鑲嵌圖形中的捷徑問題

本研究從在方格棋盤中走捷徑的問題出發,推廣至由多個相異正多邊形所組成的半正鑲嵌圖形棋盤,其沿格線走捷徑的方法數與最短路徑。研究中,我們針對所有8種1律半正鑲嵌圖形進行分類探討,包括截半六邊形、截角六邊形、扭稜六邊形、小斜方截半六邊形、大斜方截半六邊形、扭稜正方形、異扭稜正方形、截角正方形圖形。我們將每種棋盤進行「轉正」,使它對應於唯一的矩形棋盤,達到「捷徑同構」,因而原本半正鑲嵌圖中的捷徑問題就等價於方格棋盤的捷徑問題。我們將走捷徑方法數的通解分類,發現有組合數類、以及階差與指數混合兩大類,並分析康威表示法與通解的關係。

一種新的複音音樂片段相似性度量

平常聽音樂時經常有種似曾相識的感覺。為了描述這種感覺,我們展開了複音音樂片段相似性度量的研究。因為曾經使用過最長公共子序列實作卻效果不如預期,我們將音樂片段正規化後,視為座標平面上的時間、音高點對的集合,使用點對應與二分圖匹配的方法,定義兩個複音音樂片段的相似度為最大權重匹配的平均邊權。我們計算了資料集(JKUPDD)中相同、相異的音樂片段的相似性,調整算法中的參數,找出最適合的參數組合,並且透過音符之間的權重,畫出自相似度矩陣,發現樂曲中的重複片段。

In Silico Modeling of Lovastatin Analogues as Inhibitors of HIV-1 Nef Protein

Currently, no method can completely eliminate the human immunodeficiency virus (HIV) in an infected person. HIV employs an accessory protein called Nef that forms a complex with cellular AP-1, preventing detection of HIV-infected cells. Lovastatin has been recently identified to inhibit the formation of said Nef-AP-1 complex, but its effective concentration is remarked to be far higher than other Nef inhibitors. This study aims to develop a modified lovastatin molecule exhibiting higher binding affinity to the HIV-1 Nef protein than lovastatin in silico. Modified lovastatin molecules based on the interaction map of lovastatin with Nef were modeled, and flexible ligand-flexible receptor docking to the Nef binding site was performed using AutoDock Vina. Residues within the Nef binding site identified by Liu et al. (2019) to be crucial (Glu-63, Val-66, Phe-68, Asp-108, Leu-112, Tyr-115) were set as flexible. Fragment-based drug design was utilized to append molecular fragments to lovastatin in order to maximize its interactions with said crucial residues. From the fragment-based approach, molecule F4 ((1S,3S)‐8‐{2‐[(2R,4R)‐4‐chloro‐6‐oxooxan‐2‐yl]ethyl}‐3‐(hydroxymethyl)‐7‐methyl‐1,2,3,4‐tetrahydronaphthalen‐1‐yl 4‐aminobenzoate) exhibited a binding affinity of -9.0 kcal/mole, and its estimated IC50 ranges between 0.25-0.51 μM which is at least 7.5 times lower than the reported IC50 of lovastatin from literature. This study presents insights on the key modifications to improve lovastatin as an HIV-1 Nef inhibitor and pertinent information about the Nef binding site for future drug development studies.

Bio-Circular-Green Superabsorber

As the world has become concerned about the global waste crisis and global warming, there has been a surge of research within materials science to find materials that would replace plastic, such as bioplastics or biodegradable materials, in order to reduce environmental pollution. Plastics generates the microplastics that allowed them to become cross contamination enter the ocean through land, sea and river. Science research found (Lusher et al. (2017)) over 220 species of marine animals ingested microplastic, half of them are considered relevant for commercial purpose and increasing the risk of human consumption as it can induce immune response, oxidative stress, cytotoxicity, alter membrane integrity and cause differential expression of genes. Thailand is also experiencing such a challenge, as seen by the overabundance of plastic waste that might take centuries to decompose. For example, around 1680 million personal hygiene products such as diapers, sanitary napkins, and tampons are used each year. This study highlights the use of naturally accessible absorbent fibers from malva nut (Scaphium scaphigerum) (G. Don) Guib & Planch.), which is widely available and biodegradable in nature and has a low carbon footprint. This study also aimed to develop natural absorbent pads using compostable spun, external layers, and biodegradable glue. A prototype sanitary napkin with biodegradable absorbent pads was developed and evaluated for absorption ability, absorption rate, pH, and biodegradability. The absorbent material absorbed up to 19 times its weight in 2 minutes and 33 times its weight in 2 hours, which is enough for an average of 80-150 mL of menstrual blood. The prototype napkin deteriorated within 99 days, based on naked eye observation. Some signs of degradation and microorganisms growing on the prototype were also observed from scanning electron microscopic images. According to the findings, natural absorbent pads made from malva nut have the potential to be converted into sanitary napkins. Furthermore, it is proposed that the components, which include superabsorbent renewable materials, spinning compostable layer, external compostable layer and biodegradable glue, may be used in a variety of goods, including adult diaper pants, incontinence pads, and laboratory bench mats.

蘭陽溪口溼地以及五十二甲溼地水質分析與比較

本研究區域為蘭陽溪口溼地與五十二甲溼地,各選擇5和6個採樣點,檢測水體中的溶氧度、pH值、導電度、總固體溶解量(TDS)、水溫以及濁度並記錄當時氣溫。 在蘭陽溪口溼地中我們發現越靠近出海口,導電度、TDS越高。採樣點4濁度為最高,猜想可能與位置有關。五十二甲溼地中則以採樣點6的導電度、TDS為最高,濁度、pH值及溶氧量則是採樣點3最高。我們還藉由五十二甲濕地分區使用圖,比較人為因素對水質的影響。採樣點3為遊客休憩區,測得的濁度、pH值皆較高,採樣點6為生態區,測值相對較小,推測人為因素與水質有關聯。最後,在10月10日的數據中,發現蘭陽溪口溼地的導電度特別高,推測潮汐現象為可能造成此現象的因素,也是未來研究的方向。

蚊幼蟲與搖蚊幼蟲在水中分布與其血紅蛋白基因表現之關係

蚊蟲呼吸透過氣管系統,本研究假設底棲孑孓與搖蚊幼蟲具備血紅蛋白系統以擷取氧氣。戶外採集8種孑孓和1種搖蚊幼蟲,錄影觀察白腹叢蚊、竹生翠蚊和鹽埕搖蚊幼蟲長時間停留底層;家蚊、斑蚊則反覆上下至水面呼吸。顯微鏡下發現白腹叢蚊幼蟲體壁內側充滿紅點,色澤隨著富氧或缺氧而變化;然二種斑蚊則不明顯。PCR增殖6種蚊蟲和1種搖蚊血紅蛋白基因片段並建構親緣關係樹。定量RT-PCR顯示,白腹叢蚊與鹽埕搖蚊在缺氧條件下,血紅蛋白相對表現量分別增加5倍和150倍,上層埃及斑蚊和白線斑蚊則增加3.9倍和1.5倍。西方墨點法證實孑孓缺氧條件下,17-kDa血紅蛋白皆有大量表現。本研究首次確定6種蚊蟲具血紅蛋白基因,證明孑孓血紅蛋白基因在缺氧下的表現量增加,包括mRNA與蛋白質。此等反應氣候變遷,暖化導致水溫升高與低溶氧條件下,蚊蟲可能有因應環境改變的呼吸機制。未來將進一步標幟血紅蛋白在蚊幼蟲體內組織之表現與其他環境變因之影響。