全國中小學科展

2022年

纖維素內切酶GsCelA自我斷裂之探討

生質能源製程中,常將植物細胞壁中的纖維素進行水解,此反應在高溫環境中進行較具商業優勢,故開發耐高溫的纖維素酶具重要性。發現自嗜熱菌Geobacillus sp. 70PC53的纖維素內切酶GsCelA 在高溫中具高活性。初步研究發現 GsCelA 蛋白質序列 N 端後第 315 和第 316 個胺基酸間會發生自我斷裂現象,導致酵素活性及熱穩定性提升。本實驗在 GsCelA 的 N 端及 C 端分別製造定點突變,探討 GsCelA 自我斷裂性質。實驗結果顯示突變GsCelA 自我斷裂速率較低,而 EDTA 可抑制自我斷裂現象;已斷裂 GsCelA 可催化自我斷裂發生,而 N 端突變之已斷裂 GsCelA 不具此性質。整體實驗結果顯示GsCelA 自我斷裂受已斷裂GsCelA 催化且為一正回饋機制,並且本次的N端突變區段在催化GsCelA自我斷裂作用上是必要的。

Investigating the application of nanotechnology for detecting fishes hatching time

Introduction: Using advanced technologies such as nanotechnology in the food and fishery industry, as one of the most important industrial sectors of countries, has received too much attention. Traditionally, fishing and hunting have been considered important sources of supplying food. The subject and methodology: The study aims to investigate nanotechnology for detecting fish hatching time. This is a review article that collects the information from databases such as Sid, civilica, and Google Scholar. In the end, 22 papers were studied for extracting and collecting the required information from the abovementioned scientific database. Finding: After examining the food, drug, and agricultural-related papers published from 2009 to 2020, it was concluded that small Nano-sensors, controlling & monitoring systems made from nanotechnology can be installed on fishing nets, fishing rods, and other fishing equipment. These devices (Nano-sensors and controlling & monitoring systems) will help fishes so that they don’t get caught. In this way, as a fish gets close to the fishing equipment, it will receive sound, smell, or heat-based alarm. Therefore, the fish will stay away from the fishing equipment. The result: according to the finding of this study, it can be concluded that excessive fishing in the hatching time will be avoided by the application of nanotechnology in the fishing equipment. As a result, the following advantages will be secured: 1- There are lots of opportunists who misuse fish during the hatching time. With the application of nanotechnology, they will be stopped. 2- Opportunists are ambushing in different time points to misuse fish. Also, the guards might be ignorant. With the application of nanotechnology, guards are no longer required. 3- This plant is cost-effective too.

The Reproduction success of the Cyprinidae and a Claridae fish species and its impact on small- scale fisheries

To investigate the reproduction success and natural recruitment of several Cyprinidae fish and Claridae fish species in the Allemanskraal Dam. The purpose of the project included investigating whether each individual fish species studied has a successful 2020/2021 spawning season in comparison with each other. Sections of the seine net were measured along with a distance of 10 along the shoreline. The ends of the seine nets were attached to one foot and the top of the net was held by hand. Both volunteers moved in unison while covering the 10m. The volunteer in the “deep end” moved towards the shoreline creating a semi-circle while the other volunteer remained stationary. The two ends of the net were then pulled onto the shore and the fish were collected. The results found that the Labeo Umbratus and Cyprinus carpio had the most successful spawning seasons with the highest recorded numbers. These high numbers of the Labeo Umbratus can be due to the fact that the species lays a large number of eggs. The high numbers of the Cyprinus carpio is due to the lower numbers of the other fish species as previous studies have shown that the species negatively impacts the environment which could in turn negatively impact the other fish species. The Claridae gariepinus and Labeobarbus aeneusas were the lowest. The low numbers of the Labeobarbus aneusas may be due to their slow growth and late maturity rate. The Labeo capensis had an average number relative to the other species and this is due to the fact that during the sampling period the dam was at 100% capacity as this is essential for the survival of the juvenile fish. The hypothesis was accepted as the Labeo Umbratus, Cyprinus carpio and Labeo capensis all have a successful spawning season. However, due to the size of the Cyprinus carpio, they would be most suited for a small scale fishery.

閃電發言人!?—探討以雷達回波了解閃電時空分布的可能性

本報告研究台灣地區閃電與雷達回波強度的關係,期望了解雷達回波資訊成為閃電多寡代言人的可能,以及是否存在閃電活躍區及寧靜區。方法為將台灣附近區域分別以不同空間尺度 0.125 度、 0.25 度及 0.5 度、 1 小時的時間視窗將閃電及雷達資料分割。再利用 R 程式將雷達回波圖的不同像素轉化為強度數值,分析不同時間及空間下,第 95 百分位的回波強度和閃電個數對數資料的關係。 我們選取2019 及 2020 年中 8 個閃電個數高的氣象事件進行分析,發現相同時間移動視窗下及固定 空間網格點內的雷達回波強度較小時,閃電個數的對數值偏低 相反的,當雷達回波強度增加,除可能發生較少閃電個數的情況,會出現較多的閃電個數。當我們將各雷達回波強度階層對應到閃電個數對 數值大約的最高 門檻,進行線性迴歸分析,結果呈現三種不同空間尺度的網格得到的判定係數均接近 1 ,斜率稍有不同,但都約為 0.1 ,顯示此關係 可以利用雷達 回波強度進行閃電在空間時間分布的估計。 以上述經驗公式為基準,將各雷達回波 強度階層所對應到的閃電可能個數以 25% 分為低標及高標,繪出閃電寧靜區及活躍區比較,發現台灣西南部及其海域為活躍區,本島為中間區,而寧靜區的分布較不規則,但多在台灣西南、東北海域。

「氫」雲直上-影響氫氣火箭飛行的各項因子探討-Discuss the Factors Affecting the Flight of the Hydrogen Rockets

本實驗主要在研究各項因子對氫氣火箭的影響,我們查了許多影片和資料,發現許多氫氣火箭實驗都沒有記錄各項因子的數據及比較,在歷屆科展的火箭實驗中,將氫氣作為燃料的作品也寥寥無幾,所以決定嘗試這個題目作為本實驗的研究主題。 我們的研究使用電解製造氫氣與氧氣,進行燃料、造型實驗,並用電腦計算出飛行高度,希望能找出最佳飛行高度因子。我們發現各種因子的飛升高度與質量、質心、燃料與造型有很大的關聯。 希望能藉由這些實驗讓人們更認識氫氣火箭,也能藉由氫氣火箭的飛行實驗讓大家更認識、更熟悉火箭之中的科學原理,讓大家對科學更有興趣。

研發奈米材料快速降解水中偶氮染料及其自動化循環系統

本研究為開發可適用於染整廠去除有機偶氮染料使用的自動化淨水設備,於設計設備前先探討奈米零價鐵(NZVI)去除化學染料效率,並針對不同操縱變因進行實驗,了解其化學反應性質。 在淨水設備的設計上,運用區域網路間Webduino Smart開發板、行動裝置、Webduino Blockly進行串聯,進行手動同步操作或自動化控制,使染整廠不僅降低人事成本及環境負擔,也避免染料外洩產生不可逆的環境嚴重汙染,同時節省染整過程使用的水資源。 操作部分選用Webduino Blockly來直接操控Webduino,使設備能在短時間內作動,避免第三方平台問題,也可提升設備的適地性。為了使染整廠能夠以最低成本達到類實驗室環境來處理廢水,特將模組與超音波清洗機調整成物聯網模式,以公式將光照度轉換為染料濃度,進而控制NZVI投加量並上傳雲端資料留存;超音波清洗機用以模擬超音波震盪機,使NZVI投加前均勻分散,發揮最佳降解效果。 本研究亦切合聯合國17項永續發展目標(SDGs)第六項:清潔飲水及衛生設施、第十二項:確保永續消費和生產模式。

推廣圓冪定理及圓錐曲線內接四邊形判定定理之探討

在歐氏幾何和射影幾何中,相異五點可決定一圓錐曲線。若給定任意四邊形,是由四邊形的四個頂點及異於此四頂點的第五點來決定圓錐曲線,則稱此四邊形為圓錐曲線内接四邊形。 圓冪定理是一個圓內接四邊形的幾何定理,包含相交弦定理、割線定理、切割線定理等三個定理,我們將圓冪定理推廣至圓錐曲線內接四邊形。首先由圓錐截痕推廣圓內兩交弦定理,是考慮兩垂直交弦,進而推導出圓冪定理推廣一式及區分圓錐曲線種類。接著利用圓錐曲線的方向直徑、邊或對角線斜率及平行邊的切線長推導出圓冪定理推廣二式、三式及四式,推廣式是採統一與歸納方式呈現。 其次,由解析幾何推導另一種圓冪定理推廣式(推廣五式),加上圓錐曲線直徑性質,論證出二種圓錐曲線及其内接四邊形的作圖及其判定條件。最後也證明圓錐曲線內接四邊形判定定理及有趣的錐線中心軌跡圖形。

休息是為了走更遠—阿拉伯芥之耐熱記憶

阿拉伯芥的長期後天耐熱性(LAT)有如人類的「學習」,如何延長其「記憶」為本研究探討的主要目標。首先以改變熱處理間期之光週期來確立 hsp101、hsa32突變株在光照期變短下,LAT 處理後的存活率有提高之趨勢,而西方墨點法也發現 HSP101 之表現量與存活率有相同的趨勢,LAT 耐熱記憶的延續與否和 HSP101 的量息息相關。低溫降低代謝與光照期變短在存活率、西方墨點法的實驗結果相似,這顯示光照期變短可能也是透過代謝減緩,進而減少 HSP101的降解,使突變株的存活率提高。最後,花椰菜HV-427、HV-178的光週期實驗中,在進行LAT處理後,低光照下的受傷率有降低的趨勢,顯示降低光照能夠提升其耐熱性。我們期望此結果未來可應用在農業生物科技,透過更具經濟價值的方式來降低代謝進而延續LAT耐熱記憶,提升其對抗熱逆境的能力。

Preparation of a Specific Detector for Aspergillus Niger in Swimming Pools

Swimming pools are one of the transmission routes of superficial and cutaneous fungal infections. Maintenance of environmental hygiene in different parts of swimming pools is of great importance, especially the hygiene of water (1). The conventional fungal detection methods include direct smear preparation, culture, and pathological examinations. However, these methods are not fast enough or do not have sufficient sensitivity (2). Therefore, the present research introduces a novel method for detecting Aspergillus niger in pool water through creating optimal conditions for this fungus, which leads to the citric acid production by the fungus and pH changes of the related culture medium. Four experiments in 10 steps were performed to find the optimal conditions for fungal growth. According to our results, adding each of the variables sucrose, soy, and ferrous sulfate can lead to favorable results. Moreover, the shaker speed increase and fungal aeration are important. Also, we showed that soybean led to the best results compared to other variables. Considering the obtained results, including the shortened detection duration and cost-effectiveness, this method can be presented to the swimming pool owners and pathobiology laboratories as the method of choice for Aspergillus niger detection.

CONTACTLESS AND NON-DESTRUCTIVE DETECTION OF CHICKEN MEAT CONTAMINATION WITH LASER SPECKLE METHOD

Harmful microorganisms in food can cause deterioration of human health, poisoning and in some cases even death. Especially fresh meat and chicken products create a suitable environment for the growth of microorganisms in terms of the nutrients it contains, water activity and pH level. For this reason, detection of microorganisms in meat products is an important issue in terms of food safety and human health. In this project, it is aimed to detect live microorganisms in meat products, especially chicken meat, in a simple, non-destructive, non-contact and fast way using laser speckle method. Laser speckle images of healthy and stale chicken meat were taken, contrast parameter and correlation analysis of the obtained patterns were made. It was observed that the contrast parameter for staled chicken meat increased by approximately 3 times compared to fresh chicken. This increase provides an understanding of the difference between contaminated chicken and fresh chicken. Speckle density changes over time in relation to the movements of living microorganisms. Thus, the correlation in laser speckle density patterns taken from contaminated tissues is disrupted. In the measurements taken with photodiode, by analyzing the change of light intensity of the speckle patterns on fresh and contaminated tissues over time, the detection of microorganisms was made easier and more precisely without the need for image processing. The proposed measurement system is a new method that detects meat contamination with laser speckle imaging. It can be developed and made portable and can be used easily in homes. Since it is a simple, non-destructive and fast method, it can be used to determine the shelf life of meat in food distribution places and markets. In addition, it has the potential to be calibrated and used for other food products other than meat products. The system developed with this study is cheap and easy to use, and the laser speckle imaging method is used in a different field other than biomedical, contributing to the literature.