全國中小學科展

2022年

纖維素內切酶GsCelA自我斷裂之探討

生質能源製程中,常將植物細胞壁中的纖維素進行水解,此反應在高溫環境中進行較具商業優勢,故開發耐高溫的纖維素酶具重要性。發現自嗜熱菌Geobacillus sp. 70PC53的纖維素內切酶GsCelA 在高溫中具高活性。初步研究發現 GsCelA 蛋白質序列 N 端後第 315 和第 316 個胺基酸間會發生自我斷裂現象,導致酵素活性及熱穩定性提升。本實驗在 GsCelA 的 N 端及 C 端分別製造定點突變,探討 GsCelA 自我斷裂性質。實驗結果顯示突變GsCelA 自我斷裂速率較低,而 EDTA 可抑制自我斷裂現象;已斷裂 GsCelA 可催化自我斷裂發生,而 N 端突變之已斷裂 GsCelA 不具此性質。整體實驗結果顯示GsCelA 自我斷裂受已斷裂GsCelA 催化且為一正回饋機制,並且本次的N端突變區段在催化GsCelA自我斷裂作用上是必要的。

創新散熱系統-致冷晶片於電腦中的應用

1.手機及筆電已成不可或缺生活用品。但,若其充電器如果充電時間太久,可能會因為本身過熱而造成充電完畢要取回時,造成手部的燙傷。因為有此生活經驗,想改善充電器等高溫設備的散熱。 2.本研究探討電腦CPU上如果加上致冷晶片,如何加速散熱,增加使用安全外也進而提高電腦效能。 3.以電腦主機為實驗的操作器材,探究研發該散熱系統,並做為以後其他需要散熱的結構基礎,作為加速散熱的要件。目前致冷晶片在兩面溫差超越20°C時,可以使致冷晶片產生電能,接上微型馬達後,得以驅動,並產生對流散熱。 4.目前有關致冷晶片的實驗中,多數是使用致冷晶片和帕爾帖效應的關係,以通入電能使晶片兩面產生溫差。而本研究將採賽貝克效應做基礎,來探討新式散熱解決方案,使CPU產生的熱轉換成可以驅動微型馬達的電能。

糖果傳遞問題之研究與推廣

n個人圍成一圈,面向圓心,且逆時針編號1,2,……,n。一開始每人手中有一個糖果,由1號開始,逆時針分別給右邊的人一個、兩個、一個、兩個……糖果,手上沒有糖果的人必須退出。我們將此傳遞規則定義為T_1,2,同理T_(1,2⋯,p)。這個傳遞遊戲,最終會有兩種情形,第一種是由一人獨得所有糖果(成功狀態),第二種是數人間傳遞糖果且形成循環(循環狀態)。 研究後得知,在傳遞規則T_(1,2⋯,p) (p≥2)下,若p=〖p_1〗^(α_1 ) 〖p_2〗^(α_2 )⋯〖p_i〗^(α_i )⋯〖p_j〗^(α_j ) ( 為p的相異質因數),任意的n值(n≥p+1)均可唯一表示成n=(p)^t×(〖p_1〗^(s_1 ) 〖p_2〗^(s_2 )⋯〖p_i〗^(s_i )⋅m)+q (t,m∈N, p ∤〖p_1〗^(s_1 ) 〖p_2〗^(s_2 )⋯〖p_i〗^(s_i ), (m,p)=1, q=1,2,⋯,p),令S=(p^t (p-q)+(pq-1))/(p-1)+R⋅p^t,則當m=1時,最終為成功狀態,且獨得糖果者的初始編號為S;當m≥2時,最終為循環狀態,且由m人循環傳遞糖果,而此m人的初始編號是S, S+p^t 〖p_1〗^(s_1 ) 〖p_2〗^(s_2 )⋯〖p_i〗^(s_i ), ⋯⋯ , S+(m-1)⋅p^t 〖p_1〗^(s_1 ) 〖p_2〗^(s_2 )⋯〖p_i〗^(s_i )。上述公式中的R值,可透過我們研究出來的「R值迭代法」求得。更進一步,我們也找出達到成功狀態或循環狀態的最小傳遞數。

半正鑲嵌圖形中的捷徑問題

本研究從在方格棋盤中走捷徑的問題出發,推廣至由多個相異正多邊形所組成的半正鑲嵌圖形棋盤,其沿格線走捷徑的方法數與最短路徑。研究中,我們針對所有8種1律半正鑲嵌圖形進行分類探討,包括截半六邊形、截角六邊形、扭稜六邊形、小斜方截半六邊形、大斜方截半六邊形、扭稜正方形、異扭稜正方形、截角正方形圖形。我們將每種棋盤進行「轉正」,使它對應於唯一的矩形棋盤,達到「捷徑同構」,因而原本半正鑲嵌圖中的捷徑問題就等價於方格棋盤的捷徑問題。我們將走捷徑方法數的通解分類,發現有組合數類、以及階差與指數混合兩大類,並分析康威表示法與通解的關係。

The Reproduction success of the Cyprinidae and a Claridae fish species and its impact on small- scale fisheries

To investigate the reproduction success and natural recruitment of several Cyprinidae fish and Claridae fish species in the Allemanskraal Dam. The purpose of the project included investigating whether each individual fish species studied has a successful 2020/2021 spawning season in comparison with each other. Sections of the seine net were measured along with a distance of 10 along the shoreline. The ends of the seine nets were attached to one foot and the top of the net was held by hand. Both volunteers moved in unison while covering the 10m. The volunteer in the “deep end” moved towards the shoreline creating a semi-circle while the other volunteer remained stationary. The two ends of the net were then pulled onto the shore and the fish were collected. The results found that the Labeo Umbratus and Cyprinus carpio had the most successful spawning seasons with the highest recorded numbers. These high numbers of the Labeo Umbratus can be due to the fact that the species lays a large number of eggs. The high numbers of the Cyprinus carpio is due to the lower numbers of the other fish species as previous studies have shown that the species negatively impacts the environment which could in turn negatively impact the other fish species. The Claridae gariepinus and Labeobarbus aeneusas were the lowest. The low numbers of the Labeobarbus aneusas may be due to their slow growth and late maturity rate. The Labeo capensis had an average number relative to the other species and this is due to the fact that during the sampling period the dam was at 100% capacity as this is essential for the survival of the juvenile fish. The hypothesis was accepted as the Labeo Umbratus, Cyprinus carpio and Labeo capensis all have a successful spawning season. However, due to the size of the Cyprinus carpio, they would be most suited for a small scale fishery.

改良型非整數次方PID控制器設計

傳統控制器都以一次方線性模式調整系統的比例、積分、微分等三參數減少誤差。為發展更佳誤差控制模式, 本研究嘗試加入誤差變數的次方運算。我們先以程式模擬一受固定阻力的線性系統,測試誤差變數次方的可行性,利用倒立擺系統進行模擬,並使倒立擺追蹤正弦波,以印證次方模式的控制效果。 經程式模擬後,我們認為不論目標值的大小,修改誤差參數的次方模式皆可使系統響應更快,控制效果更好。後續將利用倒立擺實測確認調整誤差次方可行性,並調整倒立擺的質量分布來模擬不同情況。相較於傳統的PID控制器,本研究拓增比例參數次方,建立PID三參數的次方模式,確認具更佳控制效果的可能性。希望未來可持續強化模式,將同步PID各參數次方模式,實際應用在機器手臂、溫度控制、建築防震等工程與工業。

Introduction of Omed-Omedan Tradition Through Augmented Reality-Based Card Games To Increase The Love of Local Culture

This research is motivated by the differences in the people's perceptions, mainly youth about the omed-omedan tradition. The difference is that many people think that the omed-omedan tradition violates the norms of decency and is also called the pornography tradition. The purpose of this study is to, 1) describe the mechanism of applying omed-omedan tradition recognition attitudes through augmented reality-based card games, 2) analyz the impact resulting from the application of omed-omedan tradition recognition attitudes through augmented reality-based card games, and 3) analyze about the feasibility of application resulting from the application of omed-omedan tradition recognition attitudes through augmented reality-based card games. This research is a type of R&D research conducted at Bali Mandara State High School from July 1-September 6, 2021. The subjects of this study were the teenagers of Kaja Sesetan Indigenous Village and the object of this study was the omed-omedan tradition through augmented reality-based card games. The data collection method used is the literature studies, questionnaires, interviews, and validation questionnaires. Data is processed based on qualitative descriptive approach and quantitative descriptive approach. The results showed that the mechanism of implementing the omed-omedan tradition originated from the download of assistive devices, namely android studios and applications from AR, the impact of augmented reality-based card games was seen as very helpful in the introduction of the omed-omedan tradition and was seen as able to help increase the love of local culture, besides that the media used was also very popular by players because it was seen as interesting and very helpful. Based on the feasibility tests that have been calculated, this game is considered very feasible to implement and researchers hope that the innovations that have been created can effectively provide understanding to help out the local community about omed-omedan tradition.

在壓力下更要堅強,探討ACE2/Ang(1-7)軸與韌力的關係

背景:韌力 (resilience)指個體遭受壓力時快速調適及復原的能力,是許多疾病的保護因子但生理機轉仍不明。Angiotensin-converting enzyme 2/Angiotensin(1-7) [ACE2/Ang(1-7)]軸具有保護心血管、抗焦慮及保護神經的潛力。目前仍無ACE2/Ang(1-7)軸與韌力相關的研究。另外ACE2是SARS-CoV-2病毒進入人體的受體,相關研究在疫情下更顯重要。 目的:本研究中我們以習得無助行為模式篩選具韌力行為的大鼠(resilient, R),探討韌力鼠與非韌力鼠(non-R) ACE2/Ang(1-7)軸表現的差異。 假說:韌力鼠的ACE2/Ang(1-7)軸活性較非韌力鼠高。 結果:韌力鼠在壓力下其corticosterone (CORT)濃度與控制組無差異。韌力鼠的Ang(1-7)濃度較非韌力鼠及控制組高,暗示較高的Ang(1-7)濃度與韌性表現相關。以Ang(1-7)/Angiotensin II (Ang II)比率作為反映ACE2 轉化Ang(1-7) 功能的指標,發現韌力鼠的ACE2濃度及功能均與未主動逃跑而受電擊的時間相關,暗示其ACE2活性能因應壓力調控上升。韌力鼠的ACE2濃度雖較非韌力鼠低,但其ACE2功能則較其他兩組高。並且,Ang (1-7)濃度及ACE2功能僅在韌力鼠與ACE2濃度呈現正相關,但在非韌力鼠則無相關。暗示在壓力下韌力鼠的ACE2仍維持有效率的功能但非韌力鼠的ACE2功能則受影響。另一方面,Ang (1-7)濃度僅在非韌力鼠與CORT濃度負相關,暗示非韌力鼠個體的ACE2/Ang (1-7)]軸活性與海馬迴-下視丘-腦下垂體(HPA)軸的活性可能相關。 結論:韌力個體的ACE2/Ang (1-7)軸活性較非韌力鼠及控制組佳且可能因應壓力而調節增加。相反的,非韌力鼠個體的ACE2/Ang (1-7)軸功能較差且可能與HPA軸的活性相關。我們的研究顯示ACE2/Ang (1-7)軸扮演調節韌力的重要角色。ACE2/Ang (1-7)軸活性是否可以做為個體韌力的指標或具有增進韌力的臨床價值需進一步研究。

建立大鼠模式之新行為派典以研究跨模式注意力的動態轉換歷程 Rapid switching of attention modality in a novel cross-modal selective attention paradigm in rodents

我們的感官不斷地提供超過大腦可以處理的信息,所以需要注意力來選擇性的處理重要的感官信息。雖然研究指出,人類有能力處理在同時呈現的不同感官刺激中集中注意力在一種感官,但對於這樣的跨模式注意力如何快速移轉在感官間仍不理解。要回答這些問題,首先要建立良好的行為派典,我們才能夠從中研究跨模式注意力的轉換歷程。在本研究,我們設計了一個新的派典,允許自由行為的老鼠在同時呈現的聽覺和視覺刺激間動態地轉移注意力,並透過老鼠的行為選擇反映出他們當下的注意力焦點。我們觀察到老鼠面對同樣的聽覺與視覺刺激時,能在不同的狀況下,將注意力焦點專注於其中一個感官刺激,並忽略另外一個; 且能在單一次刺激呈現之間快速的移轉注意力焦點。這些結果證實我們建立了一個新的跨模式注意力派典,我們能在未來的實驗中研究背後的神經迴路機轉。

Study of Ferrofluid and Magnetic Fields

鐵磁流體同時具有液體的流動性和固體磁性材料的磁性,由磁性微粒、界面活性劑以及載液混合形成的膠體液體。本報告探討鐵磁流體的製備過程,在化學共沉法中改變滴入氨水的濃度 (pH 值),建立一套標準稀釋氨水 pH 值量表。為了研究產物之間的差異,將產物進行 SEM 拍攝與 XRD 分析。 本研究使用低成本自製磁力測量裝置,測量磁力數值繪製多張圖表進行分析。從對磁場研究的過程發現鐵磁流體在受磁鐵吸引時會產生類似泰勒錐之錐體,架設自製觀測設備,觀察多種變因對錐體行為之影響,同時對錐體進行數值分析。此點乃為本研究提出創新科學設計。 未來將探討粒徑對製成鐵磁流體性質的影響,並以更精確的方式探討鐵磁流體在磁場下的受力分析圖。探討鐵磁流體之導電性,分析應用於現有電漿裝置之可能性,期望改良目前的電漿產生裝置中高汙染且不可撓曲的缺點。