全國中小學科展

2021年

細胞核 CTEN 蛋白質對 Wnt 訊息傳導路徑之影響及細胞癌化之調控

正常細胞的 C-terminal tensin-like (CTEN) 蛋白質位於細胞質且含量低,但研究發現 CTEN 在大腸癌細胞中會大量表現並累積於細胞核內,進而提升癌細胞遷移與侵襲的能力;而 Wnt 訊息傳遞路徑在癌細胞中常有過度活化的現象。因此本研究主要探討 CTEN 於細胞核內的累積和 Wnt 訊息傳遞路徑的互動關係,進而調控細胞癌化的機制。分析 CTEN 分布有差異之細胞株的轉錄體發現,在較多 CTEN 累積於細胞核的大腸癌細胞株中,其 Wnt6、Wnt11 兩基因的表現量上升,研究指出 Wnt6、Wnt11 皆會影響大腸癌細胞的遷移與侵襲。另外,CTEN 會與 Wnt 訊息傳遞路徑中的 β-catenin 結合,因此本實驗利用啟動子帶有 Wnt 訊息下游轉錄因子結合位的質體,測定其下游 β-catenin/TCF/LEF 轉錄因子的轉錄活性。結果顯示 CTEN 累積於細胞核中會提高 β-catenin/TCF/LEF 之轉錄活性,進而增加 Wnt6、Wnt11 等下游基因的表現量,影響大腸癌細胞的遷移與侵襲。

探討雙黃酮類CRYT對抑制發炎反應與免疫細胞移動的影響

發炎反應與癌症具有密切的關係,而如何抗發炎是現今重要的研究主題。本研究主要探討雙黃酮類藥物CRYT對LPS誘發之發炎反應與免疫細胞遷移的影響。本研究首先以細胞試驗分析細胞激素與趨化因子的表現量觀察CRYT對發炎反應的影響。接著,利用反轉錄聚合酶連鎖反應 (RT-PCR) 和共軛焦顯微鏡觀察黏附分子與調控細胞骨架分子的表現量,以了解CRYT對免疫細胞遷移的影響。再來我們以西方墨點法分析免疫反應調控路徑之蛋白質表現量來研究CRYT的作用途徑。同時,透過程式模擬CRYT的標的蛋白,並用細胞轉染與雙冷光技術驗證模擬結果。本研究發現,CRYT會抑制TNF-、IL-1及IL-6三種促發炎因子與CXCL1、CCL3及CCL4三種趨化因子的表現量。另外,CRYT會抑制黏附分子LFA-1與VLA-4與調控細胞骨架分子Cdc42、PAK1、WASP、Rac1及LIMK1之mRNA表現量,以阻礙F-actin進行聚合作用。本研究也發現,CRYT能藉由抑制NF-B的表現量,進而影響巨噬細胞的發炎作用與細胞移動。在作用機制的方面,CRYT會結合在雌激素受體的配體結合位,並抑制雌激素受體的轉錄作用,而抑制發炎作用與細胞轉移。結論得知,CRYT不但可以抑制發炎反應,還能有效抑制免疫細胞的遷移。

Dependence of Alloy Composition in Color Change of Brass Foil by Oxide Thin Layer Formation

It is known that copper foil undergoes a color change in heating by oxide thin layer formation. Therefore, we focused on the color change by the oxidation of brass foil. Brass foil (Akaguchi (Cu87%Zn13% alloy) and Aoguchi (Cu85%Zn15% alloy)) also undergoes color change by oxidation, and it shows heating time and temperature dependence. Brass foil need longer heating time to appear color change than copper foil, and we can visually confirm that the brass has corrosion resistant. In addition, color change of brass foil depends on the percentage of copper in the brass, and Aoguchi shows rapidly color change in same heating condition. We show that brass has different physical properties than copper, even with a high percentage of copper in brass, and this was verified through comparison using diffusion length and RGB data in Aoguchi and Akaguchi. We demonstrate these colored brass foils are used as art materials, and our results expanded material using possibility of brass foil.

利用近端策略優化演算法結合內在好奇心模組進行2D雙足模型行走模擬

強化學習為當前AI領域的熱門話題,其特點是在環境的獎勵與懲罰下,進行學習。強化學習雖然較為困難,但其成功的項目都非常有名,其中最著名的例子有: AlphoGo、AlphaZero等等。 深度強化學習(DRL)是深度學習與強化學習的結合體,本專題透過DRL實現近端優化策略演算法,來使BipedalWalker環境中的二足模型學會行走,並調適超參數與神經網路來讓模型訓練擁有更好的結果。 經過實驗後發現,適當的降低獎勵折扣衰減率能有效的提升學習速度以及學習上限,同時可以避免分數落差過大導致的Dead relu問題。最終的結果能讓平均分數達到302分,成功達成了BipedalWalker環境要求(平均分數>=300分)。 為了使智能體擁有更好的探索能力,本專題加入了ICM(Intrinsic Curiosity Module),成功提升了最終的平均分數至316分,將不摔倒的機率提升至99%,最高分數則到了320分,使得雙足模型能以更快的速度向前移動並保持穩定。

AUTONOMIC HEATING GLOVES

In today's world, medicine is very advanced, thanks to which many diseases that were previously considered incurable, are now treated almost all over the world. But, unfortunately, some diseases are still incurable and can only facilitate their course. One such disease is Raynaud's disease or Raynaud's syndrome. Statistics show that worldwide the percentage of patients with this disease is 3-4%. Raynaud's disease is a paroxysmal spasm of the arteries of the fingers of the hand, rarely the feet when cooling the extremities. As mentioned earlier, this disease is incurable. That is why the creation of a device that can help people overcome many inconveniences due to the inability to stay in the cold without gloves or the problem of discomfort in heated gloves is relevant. And one of the solutions to this problem is to create special heated gloves. This work is also relevant, because even existing treatments, such as medication and conservative, do not completely solve the problem of reducing the sensitivity of the hands when cooled or even the slightest moisture on the palms. Also, these methods are very expensive, so our device will be cheaper and more affordable than existing ones. When using our gloves together with the two already mentioned methods, the treatment will be more effective. Unfortunately, medical and conservative treatments will lead to complications over time, so we not only maintain sensitivity in the hands, but also prevent further amputation of the upper extremities and the emergence of human health problems associated with the effects of drugs on the whole body. Nowadays, people work hard to be able to live well, but it is difficult for people with Raynaud's phenomenon to do so, as the sensitivity of the upper extremities decreases during the exacerbation of the disease. It is important for us to maintain the sensitivity of the hands by normalizing the thermal balance of the hands, which leads to the elimination of spasms of the atria of the hand. The aim of the work is to create a simple and effective means to normalize and maintain the thermal balance of the upper extremities, in order to reduce the loss of sensitivity of the hands in patients, as well as reduce the likelihood of spasms of the arteries of the fingers. The subject of the study is the course of Raynaud's disease and the current treatments for it. The aim of the study is the creation of special gloves that can stop spasms of arteries and maintain blood flow in them by balancing the heat balance in the hand, and depriving patients of the disease during their wearing During the work the following tasks were set: - to theoretically investigate the peculiarities of Raynaud's disease; - to analyze the existing clothes on the market with heating; - to develop and improve its own design of heated gloves, which will be affordable and easy to use. - calculate the cost of gloves taking into account all factors

新式高能磁場對於骨髓幹細胞軟骨分化的影響

近年來,再生醫療利用幹細胞幫助組織器官新生的方法已有長足進步,但仍然面臨許多的瓶頸。例如: 幹細胞注射進入人體以後,無法繼續分化生長成所需組織。最近,高能磁場的使用也是醫學界蓬勃發展的議題,我們試圖利用高能磁場誘導幹細胞的分化,希望可以增加幹細胞分化的效能,這是一創新的嘗試。我們比較新型高能磁場(RMS)與對照組於骨髓幹細胞(BMSCs)的分化效果,發現RMS 中叢集式刺激(iTBS)的SOX9和COL10的基因表現與免疫組織化學染色明顯優於15Hz RMS 與對照組。最佳的刺激設計為強度50%的iTBS連續施打五天。我們亦發現每回合900 pulses有優於每回合600 pulses的趨勢。此實驗證實iTBS能夠增加BMSCs在體外的軟骨分化表現,反映在基因表現層次到膠原蛋白合成的層次。未來,此方法將進一步使用於動物實驗,期望擴展到臨床上用於退化性關節炎的治療,對再生醫療發展有所幫助。

Imperative Programming程式碼與Functional Programming程式碼的等價性與其證明,使用Agda

本研究主要考慮在盡量保留可讀性的情況下,找出將 Imperative Programming 程式碼對應的 Functional Programming 的程式碼並證明。 結果如下: 一、if statement 等價於由 ifte 函數所構成的程式碼,其中函數ifte定義在本文內 二、某些 for-loop statement 等價於由 foldl 函數所構成的程式碼 三、某些 for-loop statement 等價於由 map 函數所構成的程式碼

Phytochemical screening and evaluation of antiangiogenic properties of sapinit (Rubus fraxinifolius) fruit crude extracts

Plants are potential low-cost alternatives for cancer treatment. Rubus fraxinifolius or “sapinit” has been found to possess phytochemicals with anti-cancer potential. This project aimed to evaluate the antiangiogenic properties of methanolic R. fraxinifolius fruit crude extracts through the chick chorioallantoic membrane (CAM) assay. Through phytochemical screening, leucoanthocyanins, phenols, and tannins were detected. For the CAM assay, 10, 20, and 30 μg/μL extracts, distilled water, methanol, and retinoic acid were applied on 60 ten-day-old chicken eggs. The CAM photographs were analyzed using ImageJ Software. The mean percent inhibitions (MPI) of total length and vascular density from both analyses were subjected to One-Way Analysis of Variance (ANOVA). The ANOVA for the MPI of total length, followed by a Tukey post hoc test, show that only retinoic acid treatment has significantly higher MPI (p = 0.0010). Meanwhile, the results for the MPI of vascular density show no significant differences between all groups (p = 0.1630). It is possible that the concentrations used in the study may not be the concentrations needed to achieve optimal antiangiogenesis. The results may also be due to the absence of phytochemicals that exhibit significant antiangiogenic properties such as alkaloids. Lower concentrations and isolated phytochemicals may also be tested.

將廢矽泥製成奈米矽以及進一步應用於鋰離子電池負極材料之研究

矽元素因具有極高的理論電容和鋰攝取量(Li Uptake)而被認為是有潛力的鋰電池負極材料,然而該材料的特性使其在充放電時體積巨幅脹縮,造成結構碎裂,縮短了電池壽命。 本研究設計一套低耗能、低成本的製程,以現今台灣光伏產業大量難以回收之廢矽泥及鎂金屬為原料,將矽之粒徑縮小為奈米級以解決上述體積膨脹問題而成為極佳的鋰電池負極材料。 我們先將矽合成矽化鎂,再利用二氧化碳及氮氣的氧化還原反應製成多孔矽,隨後再利用創新的化學蝕刻法產出奈米矽。目前業界奈米矽的製備仍以高功率研磨為主,本研究首度使用銅離子、過氧化氫及氫氟酸作為蝕刻液,成功製造出平均粒徑100nm之負極材料。而過程中所使用的鎂粉可回收再使用,回收率高達94%,故幾乎無成本廢料排放,大幅降低當今鋰電池的生產成本,也落實防廢、再生、節能等綠色化學原則。

以奈米錫奈米銦增益小分子團水製備及促進藥物傳輸效能探討

小分子團水(water cluster)的製備及應用一直是科學界的挑戰。本實驗利用熱蒸鍍法,加熱金屬塊材成原子蒸氣,在氬氣環境配合液態氮溫度下冷凝收集,成功製備了平均粒徑10nm金、20nm銀、54nm銦、71nm錫、14nm鎳奈米顆粒。將定量的奈米顆粒滲入去離子水中,以超聲波分散顆粒團聚,再以波長530奈米的綠光照射,讀取拉曼散射譜圖,判定來自小分子團水的振盪強度,探討5種奈米顆粒對形成小分子團水的功效。我們驚訝的發現奈米銦藉表面電漿共振(surface plasma resonance)及表面電子氧化還原功效,弱化水分子團簇成大分子團的功效為奈米金的16倍,奈米錫為12倍,也均高效於奈米銀及奈米鎳。以奈米銦及奈米錫增益小分子團水後,對將癌細胞藥物、養分帶入細胞的功效明顯提升。