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臺灣

「鹽」來不一樣――碳酸鹽與鹽酸反應特性之研究

本研究主要是釐清碳酸鹽類與鹽酸反應後的吸放熱現象。以實驗室常見的三種藥品碳酸鈣、碳酸鈉和碳酸氫鈉做為探討對象,並自製暗箱,結合感測器測量反應後的二氧化碳、濕度和水溫,經過反覆實驗後,發現(一)異相和均相反應的結果不完全相同,吸放熱與物質狀態有關,特別是碳酸氫鈉,不宜簡化成“酸+鹼必放熱”的論述。(二)異相反應時,碳酸鈣和碳酸氫鈉有粉塵效應,但碳酸鈉的粉塵效應不明顯。(三)比較溼度與二氧化碳的變化曲線,可說明碳酸鹽與鹽酸反應產生的水主要是以液態為主,可以清楚輔助說明化學反應式的狀態。 綜上所述,本研究自製的觀測器,不僅可以即時監測化學變化,還可驗證物質的基本反應熱和狀態的特性。

蟲以食為天一線蟲覓食與攝食行為之探討

秀麗隱桿線蟲(Caenorhabditis elegans)為在科學界備受矚目的模式生物,對於其生殖及發育過程已有諸多研究,然而對於其覓食及攝食行為的相關研究卻仍闕如;每一種生物都有其獨特的行為模式,而為了解線蟲發展出何種覓食與攝食的行為以適應自然的環境,因此著手進行研究。本研究歸納出線蟲的七種一般行為:前進、探頭、偏移、擺頭、後退、轉向及拐彎行為,並探討其意義。其中,前進及探頭行為為恆常出現。偏移與轉向行為與攝食有關,在有菌環境中出現,可避免其遠離食物來源,而連續的後退及轉向行為也構成特殊行為之樹枝狀行為,推測應為幫助線蟲的消化所出現的行為。擺頭與拐彎行為與覓食有關,在無菌環境中出現。後退行為雖在有無菌環境中皆出現,但由後退後行為的不同可以了解後退行為在兩種環境下具有不同意義。在有菌環境中,後退後通常伴隨轉向行為;在無菌環境中,後退後通常伴隨拐彎行為,為逃離不適環境且藉以覓食的行為。經過研究,發現線蟲的覓食機制為嗅覺,且對嗅覺有依賴性及專一性,在其他氣味存在的環境下會大大降低覓食的效率,但最後仍然能正確地找到食物來源。線蟲於覓食的過程中所出現的特殊行為之混亂行為,由實驗結果發現為是E. coli 所釋放至培養基的化學物質所造成,化學物質濃度越濃,線蟲所表現出的混亂行為就越明顯。推測此種現象發生的原因,應為E. coli 釋放化學物質以干擾線蟲,以避免本身受到捕食,然而,線蟲也演化出適應的能力,因此在發生混亂行為後仍然能抵達菌落,此種特殊的交互作用表現出線蟲與E. coli 間的共演化現象。Cenorhabditis elegans has long been a model organism used for developmental biology studies. However, researches about its foraging and feeding behaviors are still limited. Every organism has its unique behaviors, and this research was conducted in order to understand more on what kind of foraging and feeding behaviors were developed by C. elegans and how they helped C. elegans to adapt to the environment in which it lives. The research concluded with seven general behaviors of C. elegans : advancing, probing, slanting, swaying, backing, turning and bending, and the meanings of it. The advancing and probing behaviors are constant. The slanting and turning behaviors, which appear in presence of food, are feeding-associated behaviors, which help C. elegans avoid leaving the food source. Continuous backing and turning behaviors also make up the branching behavior, one of the special behaviors, which help C. elegans’ digestion. Swaying and bending behaviors, which appear in absence of food, are foraging-associated behaviors. Although backing behavior appear both in presence and absence of food, the difference in post-backing behaviors show that there are distinct meanings of backing behavior in each environment. In presence of food, turning after backing is usually observed; in absence of food, bending after backing is usually observed, which indicates the act of escaping from unsuitable environment and searching for food. Studies show that the foraging mechanism of C. elegans is by olfaction, and C. elegans is highly olfaction-dependent and specific: efficiency of foraging decreases significantly under environment with scents other than that of E. coli, but C. elegans is still able to locate the food. According the experiments, the chaos behavior, one of the special behaviors, appears during the foraging process of C. elegans, and is caused by chemicals released to the medium by E. coli. The density of the chemicals and the extent of chaos behavior is in direct proportion. The theory is E. coli secrets chemicals to disturb the worm to avoid being preyed upon. C. elegans, however, also evolved the ability to adapt to the chemical so that it can still reaches the food source even after chaos behavior appeared. This unique interaction is a manifestation of co-evolution between C. elegans and E. coli.

長期服用安非他命對小鼠腦部紋狀體內蛋白質表

安非他命的濫用在台灣是非常嚴重的公眾健康及社會問題。安非他命會導致一連串的行為異常,包括在中腦紋狀體內釋放多巴胺及阻止多巴胺回收來增加使用者的活動力。由於安非他命會對腦細胞造成傷害,本研究的目的為探討低劑量、無立即毒性之安非他命(類似於人類使用習慣)長期施打下,是否會對C57BL6 小鼠大腦紋狀體內的蛋白質表現有影響。因此利用西方點墨法分析施打低劑量安非他命(2 到6 mg/kg) 約一星期之後,C57BL6 小鼠的大腦紋狀體中一些重要蛋白質(包括腺.酸受體A2A-R、第五亞型腺.酸環化.AC5、caspase-8 及PARP) 的表現是否有改變。實驗結果顯示,低劑量安非他命處理對這些蛋白質的表現並沒有明顯的差異。但利用二維電泳法可看到有少許蛋白質,在經過安非他命處理下有顯著的差別,如KIAA0193 homolog 、GOS-28、gammacrystallin A、malate dehydrogenase 和phosphoglycerate mutase isozyme B (PGAM-B)。這些蛋白質中,malate dehydrogenase 和PGAM-B 與代謝和產生ATP 有關,但前者是增加的,而後者減少,推測安非他命會影響神經細胞的能量代謝,因此長期施打安非他命對紋狀體造成的影響值得進一步探討。;The wide spreading use of amphetamine (AMPH) in Taiwan has become a serious public health and social problem. AMPH evokes a series of behavior abnormality including enhanced locomotor behavior by releasing dopamine and inhibiting dopamine-uptake in the striatum. Since AMPH is known to cause brain damage, the purpose of this study is to investigate the expression of several important proteins in the striatum of C57BL6 mice after chronic treatment with low and non-toxic dosages of AMPH (mimicking the common usage pattern of AMPH addict). C57BL6 mice were daily IP-injected with various dosages of AMPH (0 to 6 mg/kg) for one week. Expression levels of A2A adenosine receptor (A2A-R), adenylyl cyclase type V (AC5), caspase-8 and PARP in the striatum were analyzed by Western blotting analysis. Most proteins examined were not affected by this 1-week AMPH treatment. By the aid of two-dimensional gel electrophoresis, expressions of a few striatal proteins (such as KIAA0193 homolog, GOS-28, gammacrystallin A, malate dehydrogenase and phosphoglycerate mutase isozyme B (PGAM-B) in AMPH-treated mice were altered. Note that malate dehydrogenase and PGAM-B are two enzymes involved in energy metabolism and ATP generation. Interestingly, the former was increased and while the latter was decreased in AMPH-treated mice. Collectively AMPH may affect the energy metabolism in neuronal cells. These results suggest that the injury induced by long-term AMPH exposure warrants our further concerns and investigation.

可旋式雙鏡頭立體相機之設計與效果分析

由現有立體相機與人眼之差異發想,本研究架設模擬可調參數之雙相機系統,拍攝兩眼影像,並在偏光式立體顯示系統平台中,定義立體影像品質,如:合成難易、凸出程度、變形程度,並讓受測者評估,以俾找出立體影像品質影響因素。研究發現:透過視線三角形模型推算拍攝立體影像時,最佳鏡頭間距約為6公分,並得以預測與控制立體影像凸出螢幕之距離。我們得到最佳鏡頭間距與影像顯示距離,也發現可旋式鏡頭對變形的影響,並藉由人因工程的研究方法,利用受測實驗得到的結果與參數,建立了一個具有可旋式鏡頭的立體相機雛型,期許未來可將可旋式鏡頭之設計積體化,進而製成一具有最佳立體影像品質的實體相機。

以Hα、K-Line、UV、Soft X-ray波段太陽影像試求太陽光球層之上的徑向較差自轉.

本研究以網路上Soft X-ray、UV195Å、K-Line、Hα波段太陽影像上黑子,光斑經度位置的逐時變量,試求太陽自光球層以上各層是否有越高層轉速越慢的現象?經試用數種方法求大量黑子、光斑位置,最後以省時,且誤差減小的"由PhotoShop軟體讀出黑子、光斑在圖檔中位置,於EXCEL軟體中套用日面座標公式",以Peter Meadows的黑子定位程式抽樣對驗,來求其經緯度值。結果:在任何緯度,光球層以上各層轉速不同(徑向較差自轉現象),但並非越高層越慢,而是在大部分緯度處其各層速度變化呈 的快慢交替現象。

量子點敏化太陽能電池中光電極應用於水裂解之產氫

本研究將探討所合成的金屬硫化物製備於量子點敏化太陽能電池之光電極,並應用於水裂解反應,使之產生氫氣作為新型替代能源。以硝酸鋅、硝酸鎘、氧化硒及硫化鈉作為起始物,利用化學浸泡法(Chemical Bath Deposition, CBD) 於氧化錫參雜氟之導電玻璃(fluorine-doped tin oxide, FTO)表面上,製備不同層數量子點硫化物之光電極。當CBD循環層數為1、5及7時,運用紫外光-可見光吸收光譜儀與掃描式電子顯微鏡分別可觀察到光譜變化,與球狀、團簇狀,以及緞帶狀奈米材料之結構。另外以線性掃瞄伏安法測量光電流,在一個太陽光強度(100 mW cm-2)照射下,當電壓為1.3 V (vs. Hg/Hg2Cl2) 時, TiO2/(CdZnS)4/(CdZnSe)2/ZnS光電極之光電流可達 79.24 mA,可證明經CBD循環製成之量子點電極具有高電催化活性。此外將沉積不同量子點的光電極與硫化鈷反電極結合後製成不同的太陽能電池,在一個太陽光強度照射下測量其光電轉化效率,當使用的光電極為 TiO2/(CdZnS)4/(CdZnSe)2/ZnS 時,可得最大轉化效率η為 2.40 %。更進一步,以此光電極在定電壓下進行水裂解反應,由自製的氫氣收集器收集氫氣,可得其與白金對電極產氫率為 0.98 mmol/hr,以此可確定最佳化的光電極。

凌波仙子─水黽蓮花效應的實驗與探討

Self Assembly Mechanism of Water Droplets

這是一系列關於水蒸氣冷凝為極細微水珠的實驗。其中可以歸納為三大部分,第一部分是基礎實驗,將水蒸氣導引至親水性介面上,觀察冷凝水珠的結構。雖然看似簡單平常,但是卻發現:不同溫度的水蒸氣,其冷凝最初始的細微珠粒,尺寸相同;爾後溫度高者,堆疊速率較大,以至於最後同時呈現的水珠大小不一,尺寸不同! 第二部分,是針對冷凝水珠自我組裝機制的探討。實驗是將水蒸氣導引至密度小於1的高分子溶液上,並藉由揮發性溶劑快速揮發,將水珠粒「分層保留」以便更深入了解「解構」後的水珠群聚機制。在這組實驗中得到兩張有趣的圖片: 在討論時,我是從對流機制切入,嘗試解構上面兩張圖。 第三部分的實驗,是將水蒸氣導引到磁場及靜電場上,觀察冷凝的機構。這部分呈現出來的結果,推翻了一般「水分子為電中性應該在電場與磁場中不受影響?」刻板觀念,實驗呈現水分子:不但在電磁場上不易長大同時也有固定的散佈模式(assembly pattern)。同時也觀察到:水分子在正電場形成的凝結水珠較為均勻,在負電場則表現出較大親水性特質。這部分的實驗對日後研究細胞膜上水分子通道應有助益。 I have tried to ask a famous math professor if he can create a formula describing the ordered array of water droplets. “Then, I should study Physics first!” He said. Condensation is the thing we live with, being found everywhere, passing without notice. But we never know when it dose start? By coalescence, water droplets grow bigger, but are not round again. We used the polymer film as template and designed the solution lighter than water, so the minute droplets will sink to the bottom and layer by layer. After seconds we may have multilayers of ordered array. This experiment presented here is actually the diary of the growth of water droplets through condensation, upon volatile fluid, magnetic field and electric field. Through convection, it discusses the self assembly mechanism of water droplets and peep into the uniformity of the size of water droplets. In this experiment, convection and magneto-electric force did play important roles in the self assembly mechanism of water droplets. The topic is mostly concerned as we are surrounded by magneto-electric waves in today’s world. This is the first step in discovering the homogeneous state of water droplets, providing insights into the self assembly mechanism of water droplets with nano sizes.

鄒之風聲-風笛

「風笛」是台灣原住民鄒族的信號用具及祈雨法器,由一條繩子綁一支竹片構成。轉動風笛時,竹片會繞繩子自轉並拍打空氣而發出聲音,並有上下飛舞的現象。風笛產生聲音的原因,為竹片拍打空氣而造成的渦流共振現象;又由於繩子扭力大小及方向改變,使風笛的音調忽高忽低、響度忽大忽小、且竹片會在兩個平面上公轉,而有週期性變化。施力使風笛公轉轉速加快時,竹片自轉速率也變快,使其音調愈高、響度愈大;而繩愈短、愈粗時,竹片的公轉週期將愈短。The wind whistler was once used by Tsou aborigines as a tool for message transfer. It is composed of a string and a bamboo flapper. When swung around, the flapper spins, beats the air, and makes sounds. Moreover, the flapper flies up and down during the revolution. The spinning flapper beats the air, causes the vortex resonance phenomenon, and thus produces sound. As the twist torque and direction change, there is periodical variation in the sound volume, sound pitch, and the movement of the flapper, which orbits up and down at two planes. If given force to speed up its revolution, the flapper,s spinning frequency also increases, which makes the sound pitch higher and the sound volume greater. Besides, when the string is shorter or thicker, the flapper,s revolution period will be shorter.

奈米碳管的成長機制與電性量測之研究

本實驗是控制奈米碳管的成長條件,研究奈米碳管的成長機制、電子場發射特性。奈米碳管的物理性質會因捲曲度(helicity)、管壁厚度(單層或多層)、管徑所影響,而不同的捲曲度,會形成不同電性(金屬性或半導體性)的奈米碳管。由此可知,探討奈米碳管的成長機制,以控制奈米碳管的穩定成長,及如何長出電性或光學性質相近的奈米碳管,是極為重要之事。為了研究奈米碳管的成長機制,我們使用微波電漿輔助化學氣相沈積法 (Microwave plasma ehanced chemical vapor deposition) ,在市售的縫衣針針尖上,成長奈米碳管,這個技術能夠經由吸收微波達到局部加熱針尖的方式,再透過控制微波功率、通入氣體的種類、壓力的變化以及催化劑的使用,能夠達到有效控制奈米碳管的成長條件。我們改變三個參數:催化劑的使用、氣體種類(Ar+CH4 或N2+CH4)及壓力,之後利用SEM 觀察奈米碳管的成長情形;再測量場發射(測量電性),討論不同成長的條件與場發射電流關係。