蟲以食為天一線蟲覓食與攝食行為之探討
秀麗隱桿線蟲(Caenorhabditis elegans)為在科學界備受矚目的模式生物,對於其生殖及發育過程已有諸多研究,然而對於其覓食及攝食行為的相關研究卻仍闕如;每一種生物都有其獨特的行為模式,而為了解線蟲發展出何種覓食與攝食的行為以適應自然的環境,因此著手進行研究。本研究歸納出線蟲的七種一般行為:前進、探頭、偏移、擺頭、後退、轉向及拐彎行為,並探討其意義。其中,前進及探頭行為為恆常出現。偏移與轉向行為與攝食有關,在有菌環境中出現,可避免其遠離食物來源,而連續的後退及轉向行為也構成特殊行為之樹枝狀行為,推測應為幫助線蟲的消化所出現的行為。擺頭與拐彎行為與覓食有關,在無菌環境中出現。後退行為雖在有無菌環境中皆出現,但由後退後行為的不同可以了解後退行為在兩種環境下具有不同意義。在有菌環境中,後退後通常伴隨轉向行為;在無菌環境中,後退後通常伴隨拐彎行為,為逃離不適環境且藉以覓食的行為。經過研究,發現線蟲的覓食機制為嗅覺,且對嗅覺有依賴性及專一性,在其他氣味存在的環境下會大大降低覓食的效率,但最後仍然能正確地找到食物來源。線蟲於覓食的過程中所出現的特殊行為之混亂行為,由實驗結果發現為是E. coli 所釋放至培養基的化學物質所造成,化學物質濃度越濃,線蟲所表現出的混亂行為就越明顯。推測此種現象發生的原因,應為E. coli 釋放化學物質以干擾線蟲,以避免本身受到捕食,然而,線蟲也演化出適應的能力,因此在發生混亂行為後仍然能抵達菌落,此種特殊的交互作用表現出線蟲與E. coli 間的共演化現象。Cenorhabditis elegans has long been a model organism used for developmental biology studies. However, researches about its foraging and feeding behaviors are still limited. Every organism has its unique behaviors, and this research was conducted in order to understand more on what kind of foraging and feeding behaviors were developed by C. elegans and how they helped C. elegans to adapt to the environment in which it lives. The research concluded with seven general behaviors of C. elegans : advancing, probing, slanting, swaying, backing, turning and bending, and the meanings of it. The advancing and probing behaviors are constant. The slanting and turning behaviors, which appear in presence of food, are feeding-associated behaviors, which help C. elegans avoid leaving the food source. Continuous backing and turning behaviors also make up the branching behavior, one of the special behaviors, which help C. elegans’ digestion. Swaying and bending behaviors, which appear in absence of food, are foraging-associated behaviors. Although backing behavior appear both in presence and absence of food, the difference in post-backing behaviors show that there are distinct meanings of backing behavior in each environment. In presence of food, turning after backing is usually observed; in absence of food, bending after backing is usually observed, which indicates the act of escaping from unsuitable environment and searching for food. Studies show that the foraging mechanism of C. elegans is by olfaction, and C. elegans is highly olfaction-dependent and specific: efficiency of foraging decreases significantly under environment with scents other than that of E. coli, but C. elegans is still able to locate the food. According the experiments, the chaos behavior, one of the special behaviors, appears during the foraging process of C. elegans, and is caused by chemicals released to the medium by E. coli. The density of the chemicals and the extent of chaos behavior is in direct proportion. The theory is E. coli secrets chemicals to disturb the worm to avoid being preyed upon. C. elegans, however, also evolved the ability to adapt to the chemical so that it can still reaches the food source even after chaos behavior appeared. This unique interaction is a manifestation of co-evolution between C. elegans and E. coli.
長期服用安非他命對小鼠腦部紋狀體內蛋白質表
安非他命的濫用在台灣是非常嚴重的公眾健康及社會問題。安非他命會導致一連串的行為異常,包括在中腦紋狀體內釋放多巴胺及阻止多巴胺回收來增加使用者的活動力。由於安非他命會對腦細胞造成傷害,本研究的目的為探討低劑量、無立即毒性之安非他命(類似於人類使用習慣)長期施打下,是否會對C57BL6 小鼠大腦紋狀體內的蛋白質表現有影響。因此利用西方點墨法分析施打低劑量安非他命(2 到6 mg/kg) 約一星期之後,C57BL6 小鼠的大腦紋狀體中一些重要蛋白質(包括腺.酸受體A2A-R、第五亞型腺.酸環化.AC5、caspase-8 及PARP) 的表現是否有改變。實驗結果顯示,低劑量安非他命處理對這些蛋白質的表現並沒有明顯的差異。但利用二維電泳法可看到有少許蛋白質,在經過安非他命處理下有顯著的差別,如KIAA0193 homolog 、GOS-28、gammacrystallin A、malate dehydrogenase 和phosphoglycerate mutase isozyme B (PGAM-B)。這些蛋白質中,malate dehydrogenase 和PGAM-B 與代謝和產生ATP 有關,但前者是增加的,而後者減少,推測安非他命會影響神經細胞的能量代謝,因此長期施打安非他命對紋狀體造成的影響值得進一步探討。;The wide spreading use of amphetamine (AMPH) in Taiwan has become a serious public health and social problem. AMPH evokes a series of behavior abnormality including enhanced locomotor behavior by releasing dopamine and inhibiting dopamine-uptake in the striatum. Since AMPH is known to cause brain damage, the purpose of this study is to investigate the expression of several important proteins in the striatum of C57BL6 mice after chronic treatment with low and non-toxic dosages of AMPH (mimicking the common usage pattern of AMPH addict). C57BL6 mice were daily IP-injected with various dosages of AMPH (0 to 6 mg/kg) for one week. Expression levels of A2A adenosine receptor (A2A-R), adenylyl cyclase type V (AC5), caspase-8 and PARP in the striatum were analyzed by Western blotting analysis. Most proteins examined were not affected by this 1-week AMPH treatment. By the aid of two-dimensional gel electrophoresis, expressions of a few striatal proteins (such as KIAA0193 homolog, GOS-28, gammacrystallin A, malate dehydrogenase and phosphoglycerate mutase isozyme B (PGAM-B) in AMPH-treated mice were altered. Note that malate dehydrogenase and PGAM-B are two enzymes involved in energy metabolism and ATP generation. Interestingly, the former was increased and while the latter was decreased in AMPH-treated mice. Collectively AMPH may affect the energy metabolism in neuronal cells. These results suggest that the injury induced by long-term AMPH exposure warrants our further concerns and investigation.
Self Assembly Mechanism of Water Droplets
這是一系列關於水蒸氣冷凝為極細微水珠的實驗。其中可以歸納為三大部分,第一部分是基礎實驗,將水蒸氣導引至親水性介面上,觀察冷凝水珠的結構。雖然看似簡單平常,但是卻發現:不同溫度的水蒸氣,其冷凝最初始的細微珠粒,尺寸相同;爾後溫度高者,堆疊速率較大,以至於最後同時呈現的水珠大小不一,尺寸不同!
第二部分,是針對冷凝水珠自我組裝機制的探討。實驗是將水蒸氣導引至密度小於1的高分子溶液上,並藉由揮發性溶劑快速揮發,將水珠粒「分層保留」以便更深入了解「解構」後的水珠群聚機制。在這組實驗中得到兩張有趣的圖片:
在討論時,我是從對流機制切入,嘗試解構上面兩張圖。
第三部分的實驗,是將水蒸氣導引到磁場及靜電場上,觀察冷凝的機構。這部分呈現出來的結果,推翻了一般「水分子為電中性應該在電場與磁場中不受影響?」刻板觀念,實驗呈現水分子:不但在電磁場上不易長大同時也有固定的散佈模式(assembly pattern)。同時也觀察到:水分子在正電場形成的凝結水珠較為均勻,在負電場則表現出較大親水性特質。這部分的實驗對日後研究細胞膜上水分子通道應有助益。
I have tried to ask a famous math professor if he can create a formula describing the ordered array of water droplets. “Then, I should study Physics first!” He said. Condensation is the thing we live with, being found everywhere, passing without notice. But we never know when it dose start? By coalescence, water droplets grow bigger, but are not round again. We used the polymer film as template and designed the solution lighter than water, so the minute droplets will sink to the bottom and layer by layer. After seconds we may have multilayers of ordered array. This experiment presented here is actually the diary of the growth of water droplets through condensation, upon volatile fluid, magnetic field and electric field. Through convection, it discusses the self assembly mechanism of water droplets and peep into the uniformity of the size of water droplets. In this experiment, convection and magneto-electric force did play important roles in the self assembly mechanism of water droplets. The topic is mostly concerned as we are surrounded by magneto-electric waves in today’s world. This is the first step in discovering the homogeneous state of water droplets, providing insights into the self assembly mechanism of water droplets with nano sizes.
鄒之風聲-風笛
「風笛」是台灣原住民鄒族的信號用具及祈雨法器,由一條繩子綁一支竹片構成。轉動風笛時,竹片會繞繩子自轉並拍打空氣而發出聲音,並有上下飛舞的現象。風笛產生聲音的原因,為竹片拍打空氣而造成的渦流共振現象;又由於繩子扭力大小及方向改變,使風笛的音調忽高忽低、響度忽大忽小、且竹片會在兩個平面上公轉,而有週期性變化。施力使風笛公轉轉速加快時,竹片自轉速率也變快,使其音調愈高、響度愈大;而繩愈短、愈粗時,竹片的公轉週期將愈短。The wind whistler was once used by Tsou aborigines as a tool for message transfer. It is composed of a string and a bamboo flapper. When swung around, the flapper spins, beats the air, and makes sounds. Moreover, the flapper flies up and down during the revolution. The spinning flapper beats the air, causes the vortex resonance phenomenon, and thus produces sound. As the twist torque and direction change, there is periodical variation in the sound volume, sound pitch, and the movement of the flapper, which orbits up and down at two planes. If given force to speed up its revolution, the flapper,s spinning frequency also increases, which makes the sound pitch higher and the sound volume greater. Besides, when the string is shorter or thicker, the flapper,s revolution period will be shorter.