臺灣

長期以來，臨床研究發現酒精會影響人類胚胎的正常發育，但是其分子機 制尚不清楚。在本研究中利用RNA 定位雜交的方式觀察酒精對於胚胎發育過程 中shh、sox9a、sox9b、col2a1、hand2 的影響，發現這些基因的表現均會受到酒 精的抑制。這項結果顯示在胚胎發育過程中，酒精透過對上述基因的影響，造成 神經脊細胞減少，細胞遷移異常，以及干擾軟骨細胞分化的現象，進而造成頭骨 發育的嚴重缺陷。此外，實驗中亦發現生長激素在腦下垂體的表現亦受到酒精抑 制。這項研究的結果成功地從基因的層次深入了解胎兒酒精中毒症候群造成頭骨 畸形及生長遲緩的病理機制。 It was known that prenatal alcohol exposure may cause serious birth defects and developmental disabilities. The molecular mechanism of this fetal alcohol syndrome still remains unclear. As revealed by whole mount RNA in situ hybridization, it was shown that expression of a number of craniofacial cartilage-related genes, including shh, sox9a, sox9b, col2a1 and hand2, were all inhibited in zebrafish embryo by alcohol exposure. It suggests that alcohol exposure may result in reducing neural crest cell production, interfering neural crest migration, preventing chondrogenesis and eventually cause craniofacial defects. In addition, the transcriptional profile of pituitary hormones were investigated by RNA in situ hybridization. It appears that only growth hormone, but not prolactin and thyroid stimulating hormone, was inhibited by alcohol exposure. The reduction of growth hormone transcription was also confirmed by real time PCR. It also appears that the expression of upstream transcription factor pit1 and downstream target gene igf1 remains unchanged. It suggests that the reduction of gh transcription is mediated by a PIT1-independent pathway. The transcriptional profile of alcohol-exposed embryo was investigated by gene microarray analysis. It appears that the expression profiles of a number of development, cellular signaling, cell growth and apoptosis related genes have be affected by 1.5% alcohol treatment. It was noted that a number of retinal-specific genes were all repressed significantly. It consists with histochemical observation that alcohol exposure results in loss lamination and disturbed differentiation. This study help us understanding the molecular mechanism of fetal alcohol syndrome.

資訊時代的來臨，促使我們的社會型態大幅改變，無一不朝數位化的方向邁進。網際網路與資訊科技的快速發展，近年來影像資料庫和數位圖書館大量的成立，關於影像資料檢索的研究已漸漸成為一門極為重要的研究議題。在本研究報告中，我們提出一種植基於向量量化編碼法的灰階影像檢索技術。向量量化編碼法是一種極為簡單的影像壓縮技術。我們應用這個編碼法，來萃取出灰階影像內容的特徵值。同時我們也計算出整張影像與影像中間位置的像素平均值，作為日後檢索影像時過濾掉影像資料庫中不需要比對特徵值的依據。\r 我們所提出的方法能有效地萃取影像內容的特徵值並且讓使用者可以快速且正確地查詢到所需要的影像。當影像資料庫中存在與查詢影像完全相同的影像時，我們的檢索技術都能在第一時間第一順位檢索出這張影像。即便影像資料庫中不存在與查詢影像完全相同的影像時，我們的檢索技術平均74.3%也能在第一時間前五個順位檢索出最相似的影像。\r \r With the coming of the information age, our sociological system change extensively, and everything has moves toward digitization. Due to the rapid development of Internet, information technology, the rapid growth of image databases and digital libraries recently, the related researches of image retrieval have become a very important issue. In this memoir, we propose an image retrieval scheme based on the vector quantization to retrieve similar images from the image database according to the pre-collected image features. Vector quantization is a very simple image compression scheme. We have applied vector quantization to extract features from grayscale image. In order to speed up the retrieval process, we also calculated the mean pixel values of whole images and the central part of the image to filter the images, which are significantly dissimilar to the query image.\r The experimental results show that our proposed approaches can effectively extract features from the image and enable users to retrieve images from image database quickly and accurately. When images stored in image database match query image, the proposed scheme can instantly retrieve the stored image at the first rank. Even though images stored in image database query image exactly, the proposed scheme can instantly retrieve the stored image over 74.3% at the first five ranks.

目前警察機關採用酚酞法檢驗有無血跡存在的可能性，我們希望研發新的試劑取代酚酞以增加靈敏度。 研發實驗分為四個階段： 一、 定性階段：實驗何種指示劑可取代酚酞法，檢驗有無血跡存在的可能性。 二、 定量階段：實驗新試劑與雙氧水之最適比例以及新試劑與不同血液樣品之反應速率比較。 三、 靈敏度比較階段：實驗亞甲藍法與酚酞法在不同濃度樣品的比較。 四、 反應機構推論階段：由實驗推論可能的反應機構並由新試劑之結構探討可能的反應機構。 實驗結果顯示，我們已成功地找到新的試劑（亞甲藍）作為初步血跡預備試驗。亞甲藍除了較容易配製、顏色不易與血液混淆，且在檢驗有無血跡時，其靈敏度亦較酚酞法高，以上優點證實此新試劑亦可做為血跡檢驗之用，作為初步血液預備檢驗。

筆者利用自製的幾丁質與不同金屬的混合溶液而燒結出的奈米顆粒成效極佳，不但粒徑大小符合，在燒結的過程中也不需經過物理研磨及介面活性劑的輔助，並且在低溫下即可燒結，可以增加實用性以及商業價值。此外，在分解亞甲藍方面也有不錯的效率，並進一步從金屬氧化物之奈米膠體混合溶液比例的不同，決定其粗糙度（以AFM測定）及能隙改變，在陽光下有更高的應用效率。而在實驗的延伸研究上，希望應用在防菌、除污、甚至光電池。 We make use of self-made chitin with the mixed solutions of different metals to solder nano particles, and the result is excellent. The particle radius not only matches up to the size, but in the process of soldering nano particles, it doesn’t need any physical pulverizing or surfactant assistance. Also, it can solder in low temperature. And we could raise its practicality and the business value. What’s more, there is a good efficiency in decomposing Methylene Blue. We can further decide the roughness (measured with AFM) and the variation of the band gap from nano colloid of different metal oxide mixed ratios.Our experiment in the extending research aims to apply it to the defense of bacteria and pollution, and even photoelectric cell.

In this experiment, we hope to produce appropriate-sized nano particles of by using the chitin. By mixing the particles with the metal of Ti, Fe and Zn of proper proportions and therefore narrow their band gaps. Thus, the Bacteriostasis of LightPhotocatalysts could appear under the environment where the energy is lower than ultraviolet ray. We use de-acetylated chitin in three ways -- chelating agent, surfactant and protecter. Then we put a thin layer of the mixture of chitin and metal nano particles on a piece of glass, and afterwards, sintering the mixture on the glass. Next, we scan the surface of the glass with AFM(Atomic Force Microscopy) to measure its particle size. The result we got showed that the surface-roughness of the Ti-Fe nano particles was 30.642nm, the best solution of all the samples. Yet, in this experiment, Fe was not suitable. Therefore we should choose the sample of Ti-Zn mixture, which is slightly smaller in roughness. According to the result we got from the experiment, we found that, under the yellow light, the survivable strain-number ratio of the sintered Ti-Fe-Zn mixture on the glass and empty glass was 0.09±0.06. This was much better than the survivable strain-number ratio of 0.17±0.06, the result we got out from the glass of pure Ti and empty glass. From the measurement, we found that the proportion of mixture could lower the excitation energy Ti needs. Through this experiment, we hope to create a layer of film containing nano particles, and by applying it to daily-use products, we could prevent harmful bacteria. 本實驗的目的，就是希望可以利用幾丁質製作出適當粒徑大小的金屬奈米顆粒，混合適當比例的鈦、鐵、鋅金屬，使其能隙變窄，讓我們能在低於紫外光能量的光譜下，產生奈米光觸媒的抑菌效果。筆者在實驗中利用去乙醯化之幾丁質在本實驗中扮演三種角色:螯合劑、介面活性劑及保護劑，以將幾丁質與金屬奈米顆粒均勻塗抹於玻璃上，並以燒結玻璃的方式進行實驗。並且利用AFM掃描玻璃表面，確認其表面尺度，驗證的結果Ti-Fe混合比例的奈米顆粒之表面粗糙度為30.642nm，為最佳狀態，但在本實驗中Fe並不適用，故應以粗糙度僅次於Ti-Fe的Ti-Zn混合比例為主。根據實驗的結果，在綠光下，混合比例的Ti-Fe-Zn玻璃與空白玻璃的菌落數比，菌落存活率為0.09±0.06，相較於純Ti的0.17±0.06來的低，代表混合比例可降低Ti所需之激發能量。經過此實驗未來筆者希望可以以幾丁質製作出一層含奈米顆粒的薄膜，應用到各種生活用品防止細菌的危害。

本研究主要針對蝴蝶之飛行進行探討，研究中主要探討蝴蝶翅膀形狀、身體重量、翅膀面積、展弦比、拍翅頻率及環境溫度對飛行速率之影響，並利用自製之風洞裝置，觀察蝴蝶之翼翅運動，分析通過蝴蝶模型之氣流方向及相關氣動力。研究結果顯示：紋白蝶展翅約4.5~5 cm，平均展弦比(AR)為1.71 ± 0.12，身體重量約為0.06± 0.02 g，翅膀面積約0.0012 ± 0.0003 m2，當紋白蝶身體重量愈重，則翅膀面積愈大(R2=0.9586)。另外，紋白蝶身體重量愈重、展弦比愈小，則飛行速率亦愈快(R2=0.5559、R2=0.4726)。23℃時，紋白蝶飛行速率為1.01±0.24 m/s，當環境溫度愈高(5、16、23℃)，則飛行速率亦愈快(y=0.07x＋0.7733，R2=0.6967)。風洞實驗發現：蝴蝶會逆風而飛，當風洞的風愈強，蝴蝶翅膀拍動角度愈大，且快而持久，仰角也變大(45 度)；蝴蝶翼尖軌跡呈八字形，翼翅運動包含線性平移及旋轉；蝴蝶拍翅時，可在翅上方及前方產生低壓帶，在後方產生高壓帶，以利蝴蝶向前方飛行。另外，翅緣彎曲角度(上反角)愈大，蝴蝶模型之上升高度亦愈高，當上反角60°時，蝴蝶模型之上升高度最高(2.2±0.1cm)。This research approaches the flying ability of butterflies. Our research mainly discusses the weight, aspect –ratio of butterflies, frequency of flapping, and the shape, surface area of butterflies’ wings, and the connection between temperature and flying velocity. More over, we use the wind tunnel which was made by us to observe the movement of butterflies’ wings and analyzed the direction of airflow and aero-elastic which pass through the wind tunnel. Our research shows that Pieris canidia’s length of wings is about 4.5 to 5 cm. The average of aspect –ratio (AR) is 1.71±0.12 . Its weight is about 0.06±0.02 . And its surface area is about 0.0012±0.0003 m 2 . The heavier Pieris canidia is, the bigger its surface area will be (R2 =0.9586). In addition, the heavier it is, the smaller its aspect –ratio will be (R2 =0.5559, R2 =0.4726), and the swifter its flying velocity will be. When it is 23°C, the flying velocity of Pieris canidia is 1.01±0.24m/s. The hotter temperature is (5,16,23°C), the swifter it flies (y=0.07x+0.7733,R=0.6967). Accroding to the detect of the wind tunnel’s experiment , the butterflies will fly on luff. When the stronger the wind of the wind tunnel is, the larger the angles of wing’s flap are. And they are fast and lasting, the elevation also becomes larger (45°). The butterflies’ trochoids of wings mimic the word “eight”, and the movement of wingspan includes parallel movement of linearity and wheel. When butterflies flap, it will amount depression upon and in front of the wings, amounting the high pressure on the back so that butterflies can fly antrorsely. Furthermore, the larger the curvy angle of marginal wings (Dihedral) is, the higher the ascending height of model butterfly will be. When dihedral is 60°, the ascending height of model butterfly is the highest(2.2±0.1 ㎝).

二十四節氣是中國古代對氣象觀測的智慧結晶，由於近幾年來聖\r 嬰與反聖嬰現象的影響，在透過嘉義氣象觀測站的協助之下，利用\r Excel 進行簡易的雨量和氣溫統計，並配合中央氣象局發布的環流型\r 態和農業災害，來探討二十四節氣在嘉義地區的適用性。\r 以陽曆來區分節氣，每年會誤差6 小時，加上四年一閏，剛好彌\r 補差距，農曆則需19 年。至於聖嬰現象對嘉義地區則呈現涼夏和暖\r 冬，但在反聖嬰現象中則變為加強四季之特性，即是熱夏與寒冬。兩\r 者都造成雨量集中夏秋兩季，形成冬春乾旱。至於二十四節氣則是以\r 夏、秋的氣溫和雨量相關的諺語較適合嘉義地區。\r Abstract\r The 24 Solar Terms, is the achievement of weather observation from\r ancient China. Because of the effects from El Nina and La Nina these\r years, we proceed simple statistics of rainfall and temperature by using\r Excel and the assistance from Chia-yi Weather Station. We adopt the\r Circulation Type and the damage of agriculture from Central Weather\r Bureau, to discuss if the 24 Solar Terms for in Chia-yi area.\r To divide the 24 Solar Terms according to the solar calendar, there’ll\r be a six –hour gap every year. The gap could be made up every four years.\r But with the lunar calendar, it takes nineteen years to offset. The El Nina\r shows cool summer and warm winter in Chia-yi area, but the La Nina\r emphasizes the characteristics of the four seasons. It shows hot summer\r and cold winter. Both of them rain mostly in summer and fall, and cause\r the drought in winter and spring. The saying of the 24 Solar Terms is\r suitable for the temperature and the rainfall during summer and fall in\r Chia-yi area.

在哺乳動物裡，硫酸化是一種參與外來物解毒作用、內生組織的荷爾蒙調節、藥物代謝及膽汁解毒之重要路徑。其中， SULTZ ( hydroxysteroid sulfotransferase ）是能進行上述反應的酵素之一，為了對 SULTZ 的功能與早期發育所扮演的角色作進一步的研究，在本實驗中，我們以班馬魚為模式動物，希望藉由原位雜交法（ in situ hybridization ）找到 SULTZ 在班馬魚早期胚胎發育的 RNA 表現位置。由目前的實驗結果，發現從卵巢到1-cell、 dome 、 50 ％一 epiboly 、 12 小時，都可在胚胎細胞看到訊號表現 · 在 24 小時、 36 小時、 48 小時、 72 小時則可在脊椎兩側體節的肌肉、內胚層、嗅窩、頭部看到訊號表現，此外，在成腦的原位雜交染色結果中亦看到了訊號表現。由此可推論 SULTZ 在斑馬魚早期發育確實扮演了重要的角色。Sulphonation is an important pathway for detoxification of xenobioties, bile acids, drug metabolism, and the regulation of endogenous hormones. SULT2 (hydroxysteroid sulfotransferase) is one of the enzymes which catalyse sulphonation. Zebrafish has emerged as a popular animal model in recent years. Compared with other vertebrates, it provides advantages including ease to get embryos, rapid external development, virtually transparent embryos and ease of genetic manipulation. The above-mentioned strong points made zebrafish a good model animal for us to understand the function of SULT2 during early embryonic development. We performed in situ hybridization to find out the RNA expression of SULT2 during zerbrafish early development. According to our present results, we can detect expression signal on the edge of telencephalon and tectum opticum. the edge on the dorsal zone of corpus cerebelli and ventral zone of periventircular hypothalamus of the adult brain. Besides, we can observe signal evenly distributed in blastocytes of the embryo at 1-cell, dome. 50%-epiboly and 12 hours after fertilization. We also find signal on the muscle next to the spinal cord during the stages of 24, 36, 48 and 72hrs. There are also expressions on hypoblast of embryos at 24, 36 and 72hrs, the olfactory pit at 36 and 4hrs, and strong expression in head region at 48 and 72hrs. These results suggest that SULT2 may have some function at the early development of zebrafish.

本次科展我們所探討的，是關於圓的分割問題，討論披薩被切出來的面積和及切痕的長度和等相關問題。 我們提出並且用較初等的數學方法證明了幾個與圓的分割有關的問題，包括披薩定理以及另外七個定理。 最後我們也利用GSP軟體驗證定理二、三、四分給2人時的對偶結果和定理二分給3人以上時的其他結果，並對於一般情形的推廣，作合理的猜測。