黑龍仔知人間冷暖
為了瞭解黃斑黑蟋蟀叫聲和溫度之間的關係,我應用物理熱平衡原理,自製水浴槽控制環境溫度,以電腦錄音程式分別錄製15℃、20℃、25℃、30℃、35℃時的蟋蟀叫聲,利用Sound Forge軟體分析並比較不同溫度下的各種特性。結果發現所分析的chirp、chirp period、syllable period、pause均和溫度呈負相關,而單位時間內的叫聲次數則與溫度成正相關,並推導出15~35℃範圍內蟋蟀叫聲次數與溫度成正相關的公式。最佳直線為:﹙15秒內叫聲次數+19.385﹚÷2.483=當時溫度﹙℃﹚,呈高度正相關﹙相關係數:0.9398﹚。本實驗也比較母蟋蟀對不同溫度下雄蟋蟀叫聲的偏好程度,發現母蟋蟀較偏愛25℃時的雄蟋蟀叫聲,此結果可能與雄蟋蟀在25℃時叫聲的波形最為穩定有關。為探測蟋蟀觸角上的溫度感受器位置,我們曾將不同部位的觸角加以剪除,結果顯示觸角剪除面積越大的雄蟋蟀越不傾向鳴叫,但無法證實是否為溫度偵測異常所導致。本實驗除供學術研究外,因黃斑黑蟋蟀在世界各地均有分布,所以希望能藉由分析溫度與蟋蟀叫聲的相關性實驗,未來嘗試能探討其在不同環境的適應行為亦或種化的可能性。另外,全球暖化問題日益嚴重,也希望能藉由相關實驗探討暖化對蟋蟀生態上的影響。最後,我們的實驗證明了蟋蟀叫聲和環境溫度確實有極大的關聯性,未來或許能參考蟋蟀感覺溫度的機制,製作出一個天然零污染的溫度計。 To find out the relationship of cricket’s calling and temperature, we use a hand-made water-bath tank to control the temperature and record the callings with microphone and software, Sound Forge. After analyzing the collected data, we’ve found that cricket’s chirp, chirp period, syllable period, and pause are indeed affected by temperature (15 degrees - 35 degrees C). Moreover, we also compare female cricket’s preference to the callings, and the result indicates that female cricket’s preference is changing with temperature. In the last, we tried to find out where the thermoreceptors are by cutting out the antennae. After cutting, crickets tend to not to make any calls at all, so we conclude the antennae might play an important role in sensing and calling. The experiment proves that this communication system is temperature coupled. Because the cricket, Gryllus bimaculatus, is a worldwide species, we may learn the accommodation or the possibility of performing a new species by researching the relationship of temperature and cricket’s callings. By the way, the Green House Effect is getting more and more serious, so we want to search for the influences on crickets that are caused by Green House Effect. The last but not the least, according to the report, maybe we can investigate the mechanism of sensing temperature and then make a natural thermometer that is no pollution in the future.
牛魔王的故鄉-台東利吉惡地之探討
本研究針對利吉惡地進行探討,研究此區之泥岩含水量、有機質含量、pH值、比重、可溶性陽離子含量、滲水特性和該區之植物種類,並探討坡度、水量對沖蝕率、山脊密度和溝痕形成之影響。 研究結果發現: 一、表層泥岩之含水率較高,深層泥岩最低。中層泥岩之有機質含量較高,表層與深層泥岩較低。各層泥岩pH值約8.1;無植被採樣點之 pH較高,有植被採樣點偏中性。有植被採樣點,其 Ca2+含量較高。 二、此區共發現十九種植物,其中銀合歡、相思樹屬優勢種。 三、坡度增加時,沖蝕率亦增加;水量增加時,沖蝕率、溝痕寬度也隨之增加,兩者呈高度正相關。第一區坡度較緩,山脊密度較大,第二區坡度較陡,山脊密度小,表面較平坦,溝痕較淺。Our research discusses with the contents of water in mudstone, the organic content, pH, the specific weight, the contents of dissoluble cation, dankness and the category of plants in Ligiligi Badland. Dissecting it`s slope, the abrasion of water, the density of mountain ridge and the formation of scuff mark. Outcome of our research: 1.Solum of mudstone is the dampest. Intermediate of mudstone has more organic content than others. Every bed of mudstone`s PH is 8.1. Having plants area is indifferent and having more Ca2+.2.We found nineteen categories of plants. For example Leucaena glauca and Taiwan acacia. 3.The more augmentation of gradient,the more increase of the abrasion of water and the breadth of the density and the abrasion of water too.
愛的教育,鐵的紀律-果蠅的學習與記憶
The main purpose of this study is to explore whether fruit flies (Drosophila melanogasters which background is 2U) can distinguish and memorize different colors and smells by learning. Futhermore, to find out the relation between age and learning ability, and analyze the influence of repeated training on how long the memory would last. According to our experiment, in smell training, the fruit flies are able to distinguish and memorize different smells, and young fruit flies have better memory than old flies. In color training, the fruit flies can distinguish and memorize different colors, but the effect was less than that of smell training. In intensive learning, proper times of learning increased the memory ability, but excessive learning exhibited an opposite effect. In the labyrinth experiment, we select and nurture the fruit flies having the best color memory in order to further understand the gene correlated with the memory learning. In addition, in one-time training, fruit flies are able to remember the smells and colors in four minutes, and young ones have the better ability. In six-time training, fruit flies have the ability to remember the smells and colors in twelve minutes. In the end, we hope you can know more about the learning and memory ability of fruit flies through our report. 本實驗主要探討果蠅是否可經由學習分辨、記憶顏色與氣味,並探討年齡與學習能力的相關性、分析多次訓練對記憶時間的影響。由實驗結果得知,氣味方面,果蠅有辨識記憶氣味的能力,以年輕果蠅的效果較佳;顏色訓練方面,果蠅亦可分辨記憶顏色;密集學習方面,適當學習次數內,有助於增加果蠅的記憶能力,但過度的學習刺激會降低學習效果。並且,我們選顏色訓練中記憶較佳的一組進行迷宮實驗,經三次的岔路選擇,選出對顏色記憶最佳的果蠅,希望能探討訓練過的果蠅子代能否有較優良的記憶。至於記憶時間方面,一次氣味學習,果蠅在四分鐘內皆有記憶,且年輕果蠅記憶效果較強,達六分鐘;另外,多次氣味學習,果蠅於第四次訓練效果最佳,且記憶能力可達十二分鐘。希望藉本實驗報告,以期更瞭解果蠅學習與記憶的資料。
無機砷與硒對染色體傷害之交互作用
中文摘要 重金屬對人體傷害的無遠弗屆-人人皆知,根據流行病學的研究指出,長期處在 重金屬含量過高之地區,易造成生理病變。比方說,長期生活在砷含量高的環境中, 易導致肝臟、周邊血管及神經系的損害,各種癌症的發生機率也大為提高。 本實驗以中國倉鼠卵巢細胞(CHO-K1)為材料,利用微核偵測技術(Michronuclei, MN)及姐妹染色分體互換(Sister chromatid exchanges, SCE)之誘引來探討亞硒酸鈉 (Sodium selenite, NaSeO2) 及亞砷酸鈉(Sodium arsenite, NaAsO2) 的交互作用 (interaction)。實驗結果顯示亞硒酸鈉本身不會造成MN 的增加,但讓SCE 頻率增加; 亞砷酸鈉會誘引增加MN 和SCE。亞硒酸鈉前處理不會減少亞砷酸鈉誘引之SCE 和 MN,但可微微降低亞砷酸鈉抑制雙核細胞(Bunucleated cells, BN)的形成,但是不明 顯。本實驗結果未能看出亞硒酸鈉前處理與亞砷酸鈉有明顯的交互作用,但發現亞 硒酸鈉會增加SCE 頻率,因此亞硒酸鈉做為保健食品值得進一步的關切及探討。 英文摘要 It is well-documented that exposure to heavy metals could cause seriously adverse effects to humans. Epidemiological evidence has shown that illness is frequently observed in residents living long-term in heavy metal contaminated area. For example, arsenic exposure was associated with increased incidences of liver diseases, peripheral vascular and neurological diseases, as well as cancers. In this report we investigated the interaction of selenite and arsenite on micronuclei (MN) and sister chromatid exchange (SCE) induction in Chinese hamster ovary-K1 (CHO-K1) cells. The present results demonstrated that sodium selenite by itself did not induce MN, but it did cause the increase of SCE frequency. Sodium arsenite significantly induced MN and SCE in CHO-K1 cells. Pretreatment of CHO-K1 cells with selenite could not reduce MN and SCE frequency induced by arsenite, but slightly prevent the cells from arsenite-induced inhibition of binucleated cell formation. In the present study, no significant interaction between sodium selenite pretreatment and sodium arsenite was observed. However, sodium selenite was unexpectedly found to enhance SCE frequency in CHO-K1 cells. The application of selenite as health protection agent warrants our concerns and further investigation.