二等獎

Up to this time we have spent almost three years in studying condensation and water droplets. Little could we have done as compared with the almighty nature. However we are rewarded by the nature as we gradually found the secrets about electro-magneto field and water droplets: The size of water droplets turn smaller upon electro-magneto field and grow more uniformly especially upon electric field. This experiment presented here is actually the diary of the growth of water droplets in condensation, upon magnetic field and electric field. Through convection, it discusses the self assembly patterns of water droplets and peep into the uniformity both of the size and the distribution mode of water droplets. In former basic experiment, we focus on temperature and the speed of water moisture; generally speaking, higher temperature speeds up the coalescence procedure but does not affects the nucleation size of water droplets in simple plain surroundings; while speed of moisture does affects the nucleation size. As we went farther, deep into convection and found magneto-electric force did play an important role in the self assembly mechanism of water droplets. The topic is mostly concerned as we are surrounded by magneto-electric waves in today’s world. This experiment anchors the first step in discovering the uniformity of water droplets in different environment, and providing insights into the self assembly mechanism of water droplets upon electro-magneto field with nano sizes. 這是一系列關於水蒸氣冷凝為極細微小水珠的實驗。其中可以分為兩大部分； 第一部分是基礎實驗。將水蒸氣導入至潔淨的介面上(蓋玻片)，觀察冷凝水珠的結構。雖然看似簡單平常，但卻有令人驚奇的發現；不同溫度的水蒸氣，其冷凝最初始的細微顆粒之尺寸是相同的 ！爾後隨著溫度的升高，堆疊速率也跟著上升；以致於最後一起呈現出來的水珠大小不一，尺寸不一。 第二部分是將水蒸氣導到磁場及靜電場上，觀察其冷凝結構。這部分的實驗推翻了一般「水分子是電中性在電磁場中不受影響？」的刻板觀念 ！實驗所呈現出來的冷凝水珠，不但於附加磁場中尺寸縮小又不易長大，同時還有固定的自我組成模式( Slef-assembly pattern);而且也發現在磁場中的冷凝小水珠的尺寸比電場中的小，可是電場中的小水珠則表現出較大的均勻特質。

1. Purpose of the research Characterization of bacterial and viral diversity of brine Tyro using molecular methods of identification. 2. Procedures For bacteria: 1. Amplification or multiplication of 16S rRNA gene (one of the most conservative gene) by polymerase chain reaction (PCR). 2. Agarose gel electrophoresis and purification of PCR product 3. Ligation of purified PCR product into the vector pGEM-T 4. Transformation of plasmids containing an insert into competent cells E.coli XB1 5. Blue-white selection (we need white colonies, they contain the insert of interest) 6. Isolation of plasmids containing an insert of interest 7. Sequencing of inserts 8. Bioinformatics analysis: matching homologues from GeneBank database, construction of phylogenetic trees, statistical analysis. For viruses: The same methods were used for gp23 gene, which code a major head protein of T-even bacteriophages; to amplify gp23 gene a special set of primers was used, along with a standard cloning protocol described above. 3. Data 1) Two libraries of clones were obtained during analysis: lake Tyro (24 operational taxonomic units (OTUs), 10 classes) and sea water (6 OTUs, 2 classes of eubacteria). 2) The most abundant classes were: gamma-, delta-, epsilon- Proteobacteria, which is in agreement with previous reports about bacteria in brines of Mediterranean Sea. 3) The number of clones was not sufficient to obtain stable estimates of diversity, the analysis require additional data. 4) The diversity of bacteria was unexpectedly high in brine but not in the seawater, due to higher and more diverse ion composition. 5) Most of the detected bacteria in the deep-sea lake belonged to the previously undescribed (18,75%) bacteria or had unusual metabolism (43,75%). 4. Conclusions The analysis demonstrated unexpectedly high diversity of halophilic bacteria inhabiting Tyro lake. Most of bacteria presented in brine water had unique and uncommon characteristics based on information about its closest relatives. Therefore, the deep-sea hypersaline lakes of Mediterranean Sea have great potential for further investigations. Preliminary results of diversity of viruses of Tyro lake were obtained during analysis, more complete description is coming soon.

我們試著尋找所需最小的城堡個數以看守整個a × b × c (a,b,c ? N) 的長方體。所謂城堡是一種棋子，當放置城堡的位置是(x, y, z) ，則(x, y,t)、(x,t, z)、(t, y, z) (t 是任何不超出邊界的正整數)是這個城堡可以看守的格子。我們用這些城堡來完全看守長方體，試著找出其最小值。在2005 年我們猜測了a = b = c 、a = b c 、a > b > c 的上界，而在2006 年時完成了a = b = c 、a = b c 的大部分情況的證明，少數不能解決的部份也提供了不錯的上界。目前我們在a = b = c 、a = b c 的情況幾乎完全解決，目前正在向a > b > c 的部份發展。A generalized searching method of finding the minimum number of castle which can oversee all over the rectangular box, defined as a × b× c (a,b,c ? N) , is presented. The castle here is defined as one kind of chess. The castle positioned as (x, y, z) can direct the lattice points of (x, y,t) 、(x,t, z) 、(t, y, z) (t is the positive integer and smaller than the box size). These castles we use here is to oversee the rectangular box and to help us to find the minimum number. In 2005, we got the upper bound of overseeing the rectangular box in the conditions of a = b = c、a = b c、a > b > c , while in 2006 we complete the proofs of the minimum number of castles based on the conditions of a = b = c 、a = b c . The further work we want to attain is to complete the case of a > b > c.

從直徑數公分的小漩渦，到直徑可達10公尺的巨型海漩；由F1~F5級的龍捲風到輕重度颱風，大自然中有各種渦流。不久之前還有雙眼颱風（杜鵑颱風）經過台灣，颱風出現雙眼時其行進路徑常有不尋常的擺動，行進方向忽而偏北，忽而偏南，擺動的頻率比一般颱風來得頻繁，又其結構紮實，對流旺盛，常造成莫大的災害。研究這個主題主要的目的是嘗試探討此雙渦流運動的起因、其運動方式和可能的控制方法。

鑒於氨氮會消耗水中溶氧，造成水質惡化、危害水域生態，且政府將逐步管制廢水的氨氮含量，處理氨氮的技術日益重要。去除氨氮的方法有物理、化學及生物法，但物理、化學法成本高且有二次汙染的風險，因此本實驗希望找到能有效分解廢水中氨氮之菌種。採取可口可樂工廠的七池汙水，以高氨氮培養基篩出七種細菌(RW、ST、ET、BA、BS、SR、CA)，並挑選出三種氨氮分解效率最佳的細菌(RW、ST、ET)。進一步發現其能有效降低廢水中氨氮，並減少氨氮廢水對水中動植物的危害。因此，我們認為RW、ST、ET能有效處理廢水中的氨氮並減緩氨氮對於環境的危害。

In this work, techniques to explore the capabilities of multi-walled carbon nanotubes\r (MWNTs) in sorting nanoparticles (NPs) were presented. A droplet of a solution comprising of quantum dots (QDs) with various sizes was deposited on an aligned array of intertwined MWNTs. Photoluminescence (PL) and fluorescence microscopy (FM) revealed that MWNTs were effective nano-sieves that could effectively sort out QDs with a size difference of ~ 2.1 nm.\r Cadmium Selenide/Zinc Sulfide (CdSe/ZnS)core-shell QDs and Cadmium Sulfide (CdS) QDs were used to explore whether chemical properties of NPs affect the sieving capability of MWNTs. Further investigation on the effects of micro-patterning on the sieving ability of MWNTs was also carried out.PL and FM results suggested that micro-patterning could aid in separation of QDs and thus improve sieving capability of MWNTs. With the above findings, QDs emitting different colors as a result of size difference could efficiently be assembled onto the MWNTs en route to three-dimensional architectures with controlled assembly of NPs.\r Together with controlled laser power to remove desired amounts of QDs decorated MWNTs, a multi-colored display could be achieved. Further experiments were also carried out to determine the feasibility of introducing MWNTs as filters for NPs. Dilute solutions containing NPs such as gold colloid was run through these MWNTs filters by gravity. Field emission scanning electron microscope (FESEM) images of the samples showed that MWNTs were successful in trapping the nanoparticles. Explorations into the length dependent effect of using MWNTs as filters, suggested that 300μm MWNTs are better nano-sieves compared to 50μm MWNTs.

本研究從1960年到2010年間臺北、臺中、日月潭、阿里山、玉山、臺南、花蓮、成功和恆春測站的時雨量資料中，發現東部測站的夜晚累積雨量多過白天，與其他測站相反，於是進一步挑選臺北、臺南和花蓮測站以探討不同地區海陸風環流對降雨量的影響。經過統計發現，花蓮測站降雨日週期的極值發生在海陸風環流發展後六小時發生，進而造成東部夜晚降雨量比白天多；而臺北測站是在二小時後發生；臺南測站則無時間落後現象。北部地區海陸風與日夜降雨變化沒有顯著的年代際變化，而東部與南部地區則可能因為地表使用改變而有變化。在季節分析方面，我們發現各地主要以夏季的海陸風與日夜降雨變化有明顯的關係。