Anaerobic Respiration: A Novel Bioelectrochemical Copper Recovery System?
Increasing concentrations of copper in discharged effluents pose hazards to aquatic food chains. This project aimed to develop a self-sustained copper remediation system based on electrical and microbiological principles. The production of electrons during yeast fermentation was investigated to catalyze the reduction reaction of dissolved copper ions. An electrical circuit was designed to harness electrons produced from either a pure or mixed culture of yeast, and were compared for voltage outputs. This system utilized a combination of carbon cloth and copper wire as the electrodes, and a magnesium sulfate based electrolyte. The better-performing cell was subjected to copper reduction analysis, in which various initial concentrations of copper were examined. Further data analysis was carried out on the voltage outputs achieved with both the mixed and pure cultures of yeast, in which an average base line was established and voltage flunctuations were compared to that of the base line. In this way, it was possible to determine the amount and severity of each voltage flunctuation — thus demonstrating whether mixed or pure cultures of yeast produced more stable outputs. Throughout the experiment, self-constructed equipment, including arduino microcontroller moderated incubators and drip-feed systems were implemented to maintain an optimum yeast growth rate. It was found that mixed yeast cultures produced smoother electrical potential outputs in response to feeding and stress intervals. The copper recovery experiment was therefore conducted using the mixed culture. Through a series of conductivity measurements indicative of copper concentrations, metal recovery was successfully demonstrated. Trend line analysis indicated similar flunctuations between voltage output and copper recovery rates, demonstrating how copper was recovered as a result of electrons harnessed from the yeast culture. These findings can be applied to the development of an energy efficient and cost-effective copper remediation system for contaminated water effluents.
The Actuator
The purpose of the Actuator is to create a practical device that passively exercises\r the lower legs to help prevent blood clots, Deep Vein Thrombosis (DVT), in\r wheelchair-bound individuals of any age. The secondary purpose of the device is to\r improve range of motion of the lower legs and speed the recovery of their leg action. It is hoped that this invention will help to prevent DVT, and allow some people to even use their legs again, through passive or active muscular motion. The invention was produced using a number of prototypes and design sketches. Although the current model is quite functional, as it keeps the users legs in motion, it is still in the prototypic design stage.\r The Actuator is a simple to use, easy to retrofit device. It is also portable and will be\r able to attach onto the front of any wheelchair. It harnesses the motion of the wheelchair to drive the users legs in a circular motion.\r The invention was tested in a closed and controlled environment: the duration of the\r Actuator’s use was constant, a doctor was present to take blood pressure and heart rate, and the rotation speed of the user’s legs was controlled by keeping the wheelchair speed constant. The data was collected by monitoring heart rate and skin surface temperature of healthy individuals, and blood pressure and heart rate of a wheelchair bound individual.\r As seen with both experiments (wheelchair bound, and healthy individuals) heart rate\r increased. In addition, the wheelchair bound individual’s heart rate also noticeably\r increased, with an evident increase in blood pressure as well. However, skin surface\r temperature is sensitive to surroundings and often provides little indication of deep vein blood flow, thus the skin surface temperature measurements were too inaccurate for any conclusions to be formulated.\r The inventor’s grandmother had developed DVT’s in 2005, and passed away because of them. Had she been given an Actuator it may have prevented such a tragedy. Her death was the driving force behind the project’s development. Over the time period that this project has been in process, the true potential of this invention has been realized.\r I truly believe that it will revolutionize the way that we treat people in wheelchairs, and the way people in wheelchairs can treat themselves.
Up the Creek
After an annual swimming rally in the Nahoon river, concerns were raised after several swimmers fell ill, complaining of possible symptoms of mild E.coli infection. Research was hence conducted to determine the following: \r \r \r The Nahoon River is safe for recreational activity in terms of faecal-coliforms and E.coli \r \r \r Whether the amount of rainfall affects these levels. \r \r \r If faecal pollution is present, to determine the possible point sources of the pollution \r \r \r To devise a method that needs no assistance from a laboratory, and could be done in a home environment at low cost. \r \r \r \r Procedures \r Colilert-18, (the reagent) contains nutrients which react to faecal-coliforms and E.coli in the sample. It causes samples to change colour when contamination is present. A dilution method (used by the Kowie Catchment Campaign) was used to test the severity of the contamination. The dilution levels were as follows: 1, 1/10, 1/100 and 1/1000. A control test was also performed. One Colilert capsule was divided among the five samples, which were incubated in a household stove at 37ºC for 18 hours. A sample was collected every four days and after heavy rainfall. \r The results were rated on a severity scale of 0-4, where a two was considered unsafe for recreational activity. If the undiluted sample remained clear, it was ra ted 0, if all the samples changed colour, it was rated as a four. The same rating system was used when fluorescing samples to test for E.coli. The daily rainfall level was compared to the levels of faecal-coliforms and E.coli. \r Conclusion \r As it has high levels of faecal coliforms and E.coli, the Nahoon River is not safe for recreational activity on a daily basis. \r Heavy rainfall causes the levels of E.coli and faecal coliforms to rise. \r The major source of the contamination is a stream entering the river from a newly developed settlement, and not a leaking sewer. \r This experiment has developed cost-effective home environment testing method which could be used in researching other rivers, marine environments, recreational waters and even drinking water by community researc