未得獎作品

這份報告的主題是藉由實驗，來探討雷射光的性質及它的應用，並實際製作利用雷射光原理的小器具。1.證明雷射光是否為單色光：先將雷射光射入不同顏色的玻璃紙，利用光無法穿透不同顏色玻璃紙的性質，以及將雷射光照射三稜鏡，觀察其反射的光線，藉此來證明雷射光是否為單色光。2.雷射光的直進性質：利用彎管或其他不透光物品將光線阻擋，觀察其是否能穿透物品，並且用不透光物質，使我們能夠看出雷射光的軌跡，來證明雷射光的直進性質。3.雷射光的折射與反射：將雷射光射入不同的物質中，觀察其折射角度，來得知雷射光在不同介質中的傳播速率不同，也觀察其是否有反射的現象。4.雷射光的應用：研究運用雷射光製成的物品，如雷射印表機、光碟機等等，並了解其製作原理。5.自行研製雷射小器具：利用雷射光的原理，來製作簡單而獨特、有效率的小器具。The topic of this report is to study the quality and applications of laser by experiments, and actually make a tool by using the principles of laser.1.Prove if laser is a one-color light: By lasing laser through a triangular prism, observe the light it reflected to prove that laser is a one-color light.2.The quality of in-line approach: By using tubes or other lightproof items to block out the light to observe if it can penetrate things, and also let us see the trace of laser to proof the quality of in-line approach.3.The refraction and reflection of laser: Study the differences of refracting angles by lasing laser into different items, to know the speed of laser in different mediums, and also observe if it will have any reflections.4.The applications of laser: Understand how laser products are made by studying them, like laser printers and CD players. 5.DIY laser tools: Make simple but unique and efficient tools by using the principles of laser.

台灣大多數學校並無全面性脊椎側彎篩檢與追蹤的健康服務，因此學生脊椎側彎的問題都不易被發現。本研究的目的希望藉由長期的追蹤與鼓勵學生進行治療，以降低脊椎側彎的盛行率與側彎角度。台灣地區學齡兒童脊椎側彎的盛行率大約是3-3.5％，而本校脊椎側彎的盛行率大約在1％左右，而且女生明顯多於男生。根據篩檢發現本校脊椎側彎較易發生在BMI（Body Mass Index）值較低的學童身上，也就是說，瘦型體格罹患脊椎側彎的機會較高。脊椎側彎可以藉由簡單的目測及儀器測量實施篩檢，並配合醫師復健運動，可達預防與治療之效果。本校實施兩年即約有60%的成效。因此建議將脊椎側彎納入學校健康檢查的項目之一（包含國小五、六年級與國中階段）。 ;The scoliosis of school children cannot be discovered easily because the overall scoliosis screening and tracing are not provided in most schools in Taiwan. The main purpose of this study is to reduce the prevalence rate and the angle of scoliosis by proceeding scoliosis screening continuously, and encouraging the patients to take physical treatment at the same time. Compared with the scoliosis prevalence rate in Taiwan(3-3.5%), the rate in our school is about 1%. Furthermore, the girls with scoliosis are outnumber the boys with the disease. This study shows children with low BMI value will have higher probability to acquire scoliosis. In other words, thin children may fall victims of the disease more often. The scoliosis can be examined with some easy methods, such as unaided-eyes measuring or scoliosis detecting. Besides screening, hospital treatment can attain the effects of prevention and cure. For the first two years, the practice of scoliosis screening in our school can improve the disease obviously (about 60%). In conclusion, the study strongly suggests the scoliosis screening can be included into an item of health examination from the fifth graders to the ninth.

This study tries to reveal how the velocity of flow influents the orientations and scattering patterns of the two-valve shells (we use Pecten in this study) during depositing processes by comparing the laboratory studies with the field observations. We learn from the laboratory experiments that the shells deposit at random directions when they are too crowded and are oriented to a certain direction when they are more scattered. We also find out that the shell fossils whose dorsal sides towards downstream with upward concaves are most likely to move. On the contrary, the shells whose dorsal sides towards upstream with downward concave are the most reluctant to move Examinations of the outcrop of the bedding of Pecten fossils, which are located near Long Dong, the northeastern tip of Taiwan, show that a lot of the shells settled along a certain direction and most of their concaves are upward. However, some of the shells with obvious orientation have downward concaves, which implies the depositional environments were under high current velocity. And the numbers of the shells whose dorsal sides are oriented to Northern East are about equal to those whose dorsal sides are oriented to Southern West. This result coincides with the results of the laboratory study. However, the experimental results can apply to the field analysis under certain assumptions.本研究就影響貝殼排列的水速作試驗，尋找水流和貝殼排列的關係，並和地層中實際的貝類化石做比較，藉以回推古老沉積環境。結果發現大量貝殼集中的情況下，貝殼間彼此的影響力會大於單純的水流沖擊；當貝殼較分散時，可以看出貝殼的頭部皆會一致朝向水流的來向作排列。而最適合移動的型態是凹口向上且頭部不朝水流來向的貝殼，而最穩定的型態是頭部朝水流來向的凹口向下貝殼。觀察實際地層，貝類化石大致上也有方向性的排列且正反面貝殼都有，可以推得當時沉積的水流大，故有足夠的動能讓貝殼重新排列。但由於試驗環境定和自然環境有所差異，仍有限制存在。

Marsilea quadrifolia, an aquatic perennial rhizomatous fern, is widespread on wetlands in Taiwan. A normal fern frond has the clusters of spore capsules on the back, yet Marsilea quadrifolia reproduces clusters at petiole base from sporocarps which are axillary, pedicellate, ovate and hairy; both microsporangia and megasporangia can be found in sporocarps. It is highly polymorphic. The sterile fronds are erect when growing in mud, and floating in water, and they produce leaflets in the shape of four-leaf clovers. Mature leaves are divided into water leaves and terrestrial leaves according to living environments. Water leaves are divided into submerged leaves, floating leaves, and emergent leaves. These four kinds of leaves have obvious differences in stoma density. The submerged leaves have the least starch which means they have the minimum photosynthetic efficiency. The flexible leafstalks allow the plants to adapt to small changes in water level, while keeping their leaves on the water surface to access light and carbon dioxide. Thus, heterophylly may be seen as an adaptive mechanism which is sensitive to some environmental parameters and that responds accordingly to maximize the capability for survival. The terrestrial leaves have periodic sleep movement according to the alternate changes of day and night. The biggest opening angle is related to a definite leaf closing time cycle. Light interference during the dark period, continuous lighting, or the reversing of day and night all interfere the opening time and speed of them.南國田字草是一種浮葉性、多年生水生蕨類，匍匐莖細長橫臥在水中泥土或濕地裡，葉四枚對生於葉柄頂端，依生長環境不同，可分為水生葉及陸生葉，水生葉又分沉水葉、浮水葉及挺水葉三種。孢子囊群聚集特化成堅硬的孢子囊果，長於葉柄基部，孢子有大、小孢子兩型。四種異型葉在內部構造、氣孔分布密度上皆有相當的差異，以適應環境的變化，葉片澱粉含量以沉水葉最少。南國田字草陸生葉隨著晝夜交替，具有週期性的睡眠運動，每天葉片開啟的最大角度和閉合時間有一定的時間範圍。黑暗期受光照干擾或連續照光、日夜顛倒，皆影響葉片的開閉時間與速率。

間歇泉是一種十分奇特的地質景觀，地下水受到火成岩入侵所帶來的穩定熱源加熱，產生水蒸氣，利用水蒸氣形成的空氣柱壓力推動水由地底噴發出地表，也因為熱源穩定使得噴發時間差接近一定值，形成所謂的「週期性」。而根據實驗結果發現，管壁較薄的管子，在噴發之後，會使管中殘餘的水稍作降溫，加上補充的冷水使系統降溫；管長愈長時，蓄水槽中的水量較多，提高溫度所需的時間也較長，而延長了噴發的週期，最後歸納出間歇泉形成及存在的三個主要條件：(1)穩定的熱源;(2)細長的孔道;(3)豐沛的地下水。 The geyser is a very amazing sort of geologic scenery. Its principle is that the heat source of invasive volcanic rock heats the underground water to produce steam that results an air-columnar with a large pressure to erupt through the underground on the surface. Because of the steady supply of heat source, the period between eruptions is closed to a constant called “periodicity.” According to the result of the experiments, the tube that have thin wall can lower the remaining water’s temperature a little and the cold water’s supply can also lower the plumbing system’s temperature after eruption. Besides, the water in the longer tube needs more time to higher its temperature to protract the period because it have more water in its reservoir. Eventually, there are 3 essential conditions of the geysers’ existence concluded: (1) steady supply of heat; (2) a thin and long standpipe; (3) abundant underground water.

秀麗隱桿線蟲(Caenorhabditis elegans)為在科學界備受矚目的模式生物，對於其生殖及發育過程已有諸多研究，然而對於其覓食及攝食行為的相關研究卻仍闕如；每一種生物都有其獨特的行為模式，而為了解線蟲發展出何種覓食與攝食的行為以適應自然的環境，因此著手進行研究。本研究歸納出線蟲的七種一般行為：前進、探頭、偏移、擺頭、後退、轉向及拐彎行為，並探討其意義。其中，前進及探頭行為為恆常出現。偏移與轉向行為與攝食有關，在有菌環境中出現，可避免其遠離食物來源，而連續的後退及轉向行為也構成特殊行為之樹枝狀行為，推測應為幫助線蟲的消化所出現的行為。擺頭與拐彎行為與覓食有關，在無菌環境中出現。後退行為雖在有無菌環境中皆出現，但由後退後行為的不同可以了解後退行為在兩種環境下具有不同意義。在有菌環境中，後退後通常伴隨轉向行為；在無菌環境中，後退後通常伴隨拐彎行為，為逃離不適環境且藉以覓食的行為。經過研究，發現線蟲的覓食機制為嗅覺，且對嗅覺有依賴性及專一性，在其他氣味存在的環境下會大大降低覓食的效率，但最後仍然能正確地找到食物來源。線蟲於覓食的過程中所出現的特殊行為之混亂行為，由實驗結果發現為是E. coli 所釋放至培養基的化學物質所造成，化學物質濃度越濃，線蟲所表現出的混亂行為就越明顯。推測此種現象發生的原因，應為E. coli 釋放化學物質以干擾線蟲，以避免本身受到捕食，然而，線蟲也演化出適應的能力，因此在發生混亂行為後仍然能抵達菌落，此種特殊的交互作用表現出線蟲與E. coli 間的共演化現象。Cenorhabditis elegans has long been a model organism used for developmental biology studies. However, researches about its foraging and feeding behaviors are still limited. Every organism has its unique behaviors, and this research was conducted in order to understand more on what kind of foraging and feeding behaviors were developed by C. elegans and how they helped C. elegans to adapt to the environment in which it lives. The research concluded with seven general behaviors of C. elegans : advancing, probing, slanting, swaying, backing, turning and bending, and the meanings of it. The advancing and probing behaviors are constant. The slanting and turning behaviors, which appear in presence of food, are feeding-associated behaviors, which help C. elegans avoid leaving the food source. Continuous backing and turning behaviors also make up the branching behavior, one of the special behaviors, which help C. elegans’ digestion. Swaying and bending behaviors, which appear in absence of food, are foraging-associated behaviors. Although backing behavior appear both in presence and absence of food, the difference in post-backing behaviors show that there are distinct meanings of backing behavior in each environment. In presence of food, turning after backing is usually observed; in absence of food, bending after backing is usually observed, which indicates the act of escaping from unsuitable environment and searching for food. Studies show that the foraging mechanism of C. elegans is by olfaction, and C. elegans is highly olfaction-dependent and specific: efficiency of foraging decreases significantly under environment with scents other than that of E. coli, but C. elegans is still able to locate the food. According the experiments, the chaos behavior, one of the special behaviors, appears during the foraging process of C. elegans, and is caused by chemicals released to the medium by E. coli. The density of the chemicals and the extent of chaos behavior is in direct proportion. The theory is E. coli secrets chemicals to disturb the worm to avoid being preyed upon. C. elegans, however, also evolved the ability to adapt to the chemical so that it can still reaches the food source even after chaos behavior appeared. This unique interaction is a manifestation of co-evolution between C. elegans and E. coli.

高效液相層析儀（HPLC）為測定中藥指標成分常用的分析工具，而近年來高效液相層析質譜儀（LC-MS）的應用，亦在天然藥物分析上逐漸扮演重要角色。本研究利用HPLC 分析人參中的12 種人參皂素，並以 LC-MS 確認各化合物。 本研究分為三個部份，第一部份為開發適當的HPLC 分析方法分析人參中的主要成份，並使用LC-MS 確認這些化合物。人參中的12 種人參皂素( ginsenosides Rb1, Rb2, Rc, Rd, Re, Rf, Rg1, Rg2, Ro and malonylginsenosides mRb1, mRb2 and mRc ) 可於60 分鐘內成功分離，在 HPLC 分析方法中使用Cosmosil 5C18 管柱，以200nm 偵測，並以20mM KH2PO4-CH3CN 進行梯度沖提， 在LC-MS 分析中則以20mM CH3COONH4-CH3CN 進行梯度沖提。 第二部份為利用上述分析方法，比較同批人參藥材進行不同處理方式後，所得人參皂素含量的多寡，包含泡茶、煮茶、泡酒等。結果顯示若為切段（飲片）泡茶，至少應浸泡30 分鐘，切段（飲片）水煮則以15-20 分鐘較佳，粉末（茶包）泡茶的變化不明顯，而切段酒浸可以得到最多量的人參皂素，且浸泡越久含量越高。 第三部份為使用多變量統計軟體SPSS 進行區別分析（DA），統計結果可作為人參藥材基原辨識之依據。 High-performance liquid chromatography (HPLC) is a common analytical tool that has been used to analyze the index components of Chinese herb medicine. In recent years, high-performance liquid chromatography-mass spectrometry (LC-MS) gradually plays an important role. In this study, we used HPLC to detect 12 ginsenosides in Ginseng Radix, and used LC-MS to identify the 12 compounds. This study includes three parts. The first part concerns the development of HPLC method for determining the chemical constituents in Ginseng Radix, and using LC-MS to identify them. In Ginseng Radix, components such as ginsenosides Rb1, Rb2, Rc, Rd, Re, Rf, Rg1, Rg2, Ro and malonylginsenosides mRb1, mRb2 and mRc could be separated successfully in 60 mins. They were detected at 200nm by using a Cosmosil 5C18 column with a linear gradient elution of 20mM KH2PO4-CH3CN in the HPLC method, and with a linear gradient elution of 20mM CH3COONH4-CH3CN in the LC-MS method. In the second part, we used this method mentioned above to evaluate the different results from various processes, which include brewing snippets in hot water, brewing powders in hot water, cooking snippets with boiling water, and brewing snippets in rice wine. Our results are listed as follows, First, the tea using Ginseng Radix snippets brewing for about 30 mins in hot water has the best result. Second, using Ginseng Radix snippets cooking for about 15-20 mins with boiling water has the best result. Third, using Ginseng Radix powders brewing in hot water has no apparent difference. Finally, using Ginseng Radix snippets brewing in the rice wine can get most ginsenosides. The third part is using the statistic software SPSS to proceed the Discriminate Analysis（DA）. The results of the statistic analysis can help us to identify the origin of the herb medicines.

隨著科技的發達，工業廢棄物應運而生，在地小人稠的台灣，每年就產生約6000 公噸的廢觸媒，為了讓環境免於遭受廢棄物的污染，本實驗著重於廢觸媒的再利用，探討廢觸媒作為混凝土中礦物摻料對水泥(砂)漿流動性的影響。 本實驗所採用的廢觸媒來自石油裂解廠的靜電集塵廢觸媒，主要由SiO2 及Al2O3 所組成且具有部份非結晶相和卜作嵐材料之特性，外觀為乳灰白色粉末、含水量2.8﹪，溶於水中有放熱現象，pH 值為3.85。廢觸媒對水的親和力大，添加至水泥(砂)漿中會使得漿體的流動性減小，因此必須藉由提昇強塑劑劑量才能提高工作度。 在水膠比(W/B)為0.42 時，隨著強塑劑HPC-1000 劑量的增加，水泥漿的流度明顯增加，而且適當量的廢觸媒取代水泥，能維持水泥漿一定的流度。當強塑劑HPC-1000 劑量為3.0﹪、砂/水泥＝2.75 時，隨著水灰比(W/C)的增加，水泥漿的流度也明顯增加，而同條件下，不論水膠比(W/B)0.485 或0.55 時，廢觸媒取代水泥之水泥砂漿的流度比飛灰、爐石、高嶺土好，與矽灰相當，其最佳取代水泥量為15﹪。 As technology continues to develop, a considerable amount of industrial wastes have been produced.In Taiwan, over 6,000 tons of spent catalysts were brought out yearly. If such wastes can be reused in some ways, it may be helpful to protect our environment from being polluted. This study was taken to determine the influence of spent catalysts on the flowability of mortar while the catalysts were used as mineral additives for concrete. Since the catalysts could reduce the flowability of cement pastes because of the affinity between the catalysts and water, it was necessary to have superplasticizers added to improve the fluidity of the mortar. The spent catalysts used in this study were spent electrostatic precipitator cracking catalysts, called Epcat, which were provided by petroleum refining companies. Comprising mainly silicon oxide and aluminum oxide, the off-white catalyst powder present water content of 2.8%, pH value of 3.85 and exothermic reaction with water. In this study, it was found that while the ratio of water to binder (W/B) was 0.42, the flowability of the cement pastes increased with the increasing addition of superplasticizers. Besides, the flowability of cement pastes kept steady while a proper amount of Epcat was used to replace cement.When the ratio of sand to cement (W/C) was 2.75, with 3.0% superplasticizers HPC-1000 added,the flowability of the mortars markedly increased while the W/C increased, and the replacement with spent catalysts made the mortars show similar flowability as silica fume but better flowability than fly ash, slag and kaolinite, no matter the W/B value of the cement pastes was 0.485 or 0.55. In fact, the best Epcat replacement for cement was 15%.

This research is mainly in observation with Caenorhabditis elegans ’s genetic mutation caused via nervous system abnormal character. In the study, I the sample have been cultivated purified and add some chemical material EMS to speed up C.elegans mutation. Then based on the character to further analysis what causeof gene deal with mutation and observe the effects in heredity. The research has two stages, on the first stage of study the mainly target is to both search and purify the mutation of C.elegans. The second stage is based on the exploration of mutation’s searching andpurifying. Because the certain mutation bodies aren’t easy to find out, the project is still on progress at the beginning of second stage, and we conclude some heredity special cases in preliminary of study. 這個實驗主要是觀察並針對線蟲因為基因的突變所產生的神經系統異常的變異性狀，在實驗中我先將樣品線蟲培養並純化至一定數量，並加入適當藥劑EMS造成其突變，經篩選並分析此性狀，進而找出造成其突變之基因，以及觀察此性狀對遺傳表現所造成的影響。 該計畫分成兩階段，第一階段的實驗重點是在突變株的搜尋以及純化上，第二階段則是在突變基因的探討上，由於特定突變株的搜尋並非容易，所以目前計畫只進展至第二階段的遺傳實驗初期，對於其遺傳特徵與突變形式上已有了初步的分析，但尚未定位出該基因的位置。

Mirabilis Jalapa is a wild flower, which is often seen in countryside of Taiwan. This plant blossom regularly every day around PM 3:00-6:00. Since its regular behavior, it has the potential to become a model plant for research on circadian ryhtem. According to the on spot observation, a Mirabilis Jalapa will bloom consistently, whereas a minority of flower will bloom earlier or later. First, we observe how Mirabilis Jalapa response to disparate environmental conditions. Under a short period of light(8h) and an average period of light(12), Mirabilis Jalapa acts as normal. But under a long period of light, it flowers approximately 1h ahead of time. When under a continuous light, Mirabilis Jalapa shows a regular flowering in the first four day, and after that, circadian rythem of flowering terminate. The ripe flower buds mentain unbloom for 10 days or more, finally end up withered. Meanwhile, floral meristems are also suppressed. Temperature alter seems affct the flowering slightly. Second, we use SDS-page to detect protein divergence 6h interval.But as many of the earlier attempt, the floral stimuli is not sensible for average protein qualitative analysis. So we decide to take more direct approch by genetic experiment. From supplemental material of article” Orchestrated Transcription of Key Pathways in Arabidopsis by the Circadian” we obtain a group of genes regulared by bioclock in Arabidopsis.With Bio Informatics we choose 41 single gene, and compare with sequences in other species, designing promotors to isolate similar genes in Mirabilis Jalapa. Use rt-PCR to detect the level of certain gene expression,and compare characters of those genes in Mirabilis Jalapa with those genes in Arabidopsis. We also blast those 453 genes to the database of animal, trying to recognize some genes that is widely separated between disparat species. And test these genes in Mirabilis Jalapa to confirm this result.本實驗先針對紫茉莉開花生物時鐘的特性進行觀察，並以不同的變因測試對紫茉莉開花時鐘的影響，紫茉莉個別花朵開花生物時鐘並不一致，長日照會對植株造成開花時間提早（約1 小時）的影響，全日照則會對花芽分生及開花行為造成抑制，溫度對於開花影響不明顯。而後利用蛋白質電泳對不同時間點的樣本進行分析，由於開花激素存量極微，故並電泳結果無明顯差異。再來以阿拉伯芥已知與生物時鐘有關的基因中經過生物資訊（Bio Informatics）預測在紫茉莉中存在與開花及光週期有關之基因（CAA75629 CAB56039 AAB60305 AAC49807 AAA82068），針對該基因進行表現量的測定，驗證該基因與開花生物時鐘的關聯性。針對動物界與植物界中已知與生物時鐘相關的基因進行交叉比對，希望找出跨界廣泛存在的生物時鐘基因，接著測試紫茉莉中該基因的存在與否及表現狀況與其他物種的差異，驗證該基因分布的廣泛性。