Investigating the Effects of Temperature and Carbon Dioxide Levels on Nannochloropsis oceanica Using a Hemocytometer Counting Method
Climate changes that include ocean acidification and global warming are serious problems in the ecosystem, affecting marine phytoplankton, including Nannochloropsis oceanica. In the effort to further explore the impact of rising temperature and carbon dioxide (CO₂) concentrations on oceanic ecosystems, the phytoplankton Nannochloropsis oceanica was used as a model organism. This study explored the effect of temperature change and CO₂ concentration on the growth of Nannochloropsis oceanica, achieving 243 samples that were tested with three different temperatures (24 degrees Celsius (°C), 28°C, 32°C) and CO₂ concentrations (0 milliliter (ml)/min, 0.4 ml/min, 0.6 ml/min), utilizing a hemocytometer counting method. Results indicate that the CO₂ concentration has a significant effect on the population of Nannochloropsis oceanica. But the temperature doesn't affect a lot. The Nannochloropsis oceanica in the lowest temperature and highest concentration of CO₂ in its environment had the highest population growth, and in the highest temperature and lowest concentration of CO₂, it had the lowest population growth. Results show the serious negative effect of climate change on the cosystem and the importance of environmental protection. Population blooms due to excess CO₂ taking up ocean resources causing dangerous ecological imbalances.
King's Power - The Utilization of Agricultural Waste in the Production of Sustainable Dry Cells
The idea of dramatically reducing the cost of the production dry cell, reducing its carbon footprint, and being able to be an alternative to current materials such as biochars really propels the interest of performing this project research. Biochars from durian husk, bamboo and coconut shell are promising alternative chemical materials of the anodes in the dry cell due to their eco-friendly traits and availability in the trophic areas which covers about 40% of the land on earth. Using the technique of pyrolysis, the latest and the best technique to produce a high carbon content biochars, the dry cell uses the potassium hydroxide as the electrolyte and manganese dioxide as the catalysts that make the biochar mixture to produce maximum voltage of 65% from the dry cell sold in the current market. The voltage analysis of the biochar dry cell was done in our school science laboratory and then, characterization tests analysis was carried out on the products from the specific biomass namely the SEM/EDX analysis, at the Material Characterization Laboratory (MCL), Department of Chemical and Environmental Engineering, Faculty of Engineering, University Putra 馬來西亞. Based on our research, the biochar obtained from the raw materials (Durian Husk, Bamboo and Coconut Shell) had shown different characteristics. The bamboo biochar had shown the most amount of carbon content which is 86.64% more than the durian husk biochar (72.77%) and coconut shell biochar (65.57%). On the other hand, based on the micrograph, we observed that the durian husk biochar had shown much created pores rather than bamboo biochar and coconut shell biochar. In our study, we found out that the average voltage produced by the three different biochars have shown that Durian Husk char dry cell produced the highest voltage which is 0.97V, more than the bamboo char (0.62V) and coconut shell char (0.73V). In conclusion, the biochar dry cell produced are much cheaper in term of its production as our biochar dry cell uses biomass that are freely available and comes from renewable source of energy, the best ingredient for Green Technology.
Quantitative environmental DNA metabarcoding for the enumeration of Pacific salmon (Oncorhynchus spp.)
Understanding species abundance is critical to managing and conserving planetary biodiversity. Pacific salmon (Oncorhynchus spp.) are keystone species of cultural, economic, and ecological importance in Alaska and especially Southwest Alaska. Traditional methods of enumerating salmon such as weirs and visual surveys are often costly, time-intensive, and reliant on taxonomic expertise. Environmental DNA (eDNA), which identifies and quantifies species based on DNA they shed in their habitats, is a potential cost- and time- saving alternative. The relative ease of collecting eDNA samples also enables citizen scientist involvement, expanding research coverage. Currently, more research is required to define eDNA’s potential and limits. This project investigates whether quantitative eDNA metabarcoding can accurately quantify the abundances of six fish species: the five Pacific salmon species plus rainbow trout. Water samples were collected from eight creeks in the Wood River watershed of Southwest Alaska. eDNA metabarcoding and subsequent bioinformatics processing produced a read count for each species. These were compared to visual survey counts, taken to be the true counts for the purposes of this study. Data analyses showed a positive, linear relationship between visual survey count and eDNA count for sockeye salmon. The regressions were significant for both the early (p = 0.089) and late (p = 0.030) sampling dates when 𝛼 = 0.10. eDNA detections of non-sockeye species generally corresponded to visual survey observations of species presence or absence. Overall, the results of this study support eDNA’s potential to be an alternative or supplement to standard methods for the enumeration of fish species.