有毛!沒毛!哪個好!?探討石田螺及其螺殼上附生藻類與環境因子之關係
This research is about two ponds in the Ｂ park’s and the Ｄ park’s snail(Square Mystery Snail：Sinotaia quadrata) in Taipei city of Nei-hu District for research object, carry out the study of the following research proceed: 1.Discriminate the algae species that are growth on the snail shell and which is a kind of interaction with the snail; 2.The influence of the snail and algae with difference of temperature, salinity, pH value and dark ; 3. The factors affect algae growth on snail shell; 4.Use the variation of snail and algae to be a biological incator. The result manifestation: the algae that are growth on snail shell have two kinds, one is Cyanophyta and the other is Cladophora sp. The interaction between algae and snail belong to communalism, but under the condition of lacking of food, the snail then will eat the Cladophora sp. which grow on the shell of other snails. The temperature adapts aspect, upper limit of the feat existence of the snail should be low in 28℃. When over than 28℃, Cladophora sp. as the most strong, Cyanophyta is secondly, and the snail then is most poor. For the maximum tolerance of the salinity, the snail is about 4.375?, Cyanophyta is about 5.0?, Cladophora sp is then about 7.0?; Under the different salinity for the tolerance , the Cladophora sp. still the most strong, Cyanophyta is secondly, and the snail then is most poor. Under the dark environment, the speed of Cyanophyta begin to be bleaching is very fast than the Cladophora sp.. In the tolerance of pH value range: The snail is about pH=5～10, Cyanophyta is about pH=7～8, Cladophora sp. is about pH=6～8; When the pH value range is in the pH=5～8, the speed of the Cyanophyta occur changing is very fast than Cladophora sp.. The algae are growing on snail shell very different between two ponds, the main reason is water pH value dissimilarly: When pH value over than 8.5, there is no Cladophora sp. to grow on the snail shell, after the pH value to decrease, Cyanophyta then will compare early than Cladophora sp. to grow on the snail shell. Calculate by the classification of the freshwater biological incator : Two organic pollution degree of the ponds may be lain in theβ-mesosaprobic to theα-mesosaprobic, and the polluting degree of the Ｄ pond is more seriously. As for two ponds, have already faced what level of eutrophication? Belong to actually which stage of pollution grade? Not only added the classification data of floating and fixative algea in two ponds, and also according to the parts of chemistry analysis method measure of the data makes the substantial evidence, then could carry out the more accurate and thorough study in the days to come steadily studying process.本研究是以臺北市內湖區兩個綠地公園(Ｂ公園與Ｄ公園)池塘內的石田螺(Sinotaia quadrata)為研究對象，進行以下研究目的之探討：1.鑑別石田螺螺殼上藻類的種類及其與石田螺的互動關係；2.溫度、鹽度、酸鹼值及黑暗等環境因子的差異，對石田螺及螺殼上附生藻類的影響；3.影響藻類附生於石田螺螺殼上的因素；4.將石田螺及螺殼上附生藻類的變化作為監測環境因子或水質變異的指標現象。結果顯示：附生於石田螺螺殼上的藻類有藍綠藻(Cyanophyta)與剛毛藻(Cladophora sp.)兩類；與石田螺的互動關係應屬於片利共生(communalism)，但在缺乏食物的情況下，石田螺則會採食同伴殼上的剛毛藻。溫度適應方面，石田螺適宜生存的溫度上限應低於28℃，超過28℃水溫環境的耐受程度，是以剛毛藻為最強，其次是藍綠藻，而石田螺則為最差。對於環境鹽度最大耐受度方面：石田螺約為4.375??，藍綠藻約為5.0??，剛毛藻則約為7.0?；在不同鹽度環境下，鹽度的耐受程度，仍以剛毛藻為最強，其次是藍綠藻，而石田螺則是最差。在黑暗環境下，藍綠藻褪色產生白化現象的速度明顯地比剛毛藻要快了許多。在環境酸鹼值耐受的範圍方面：石田螺約在ｐＨ＝5～10 之間，藍綠藻約在ｐＨ＝7～8 之間，剛毛藻則約在ｐＨ＝6～8 之間；而酸鹼值範圍在ｐＨ＝5～8 時，藍綠藻產生變化的速度明顯地比剛毛藻還要快。而兩樣區池塘水體酸鹼值的不同，應是造成石田螺螺殼藻類附生現象差異的主要原因：當酸鹼值超過8.5 時，螺殼上就無剛毛藻附生，當酸鹼值降下後，藍綠藻則會比剛毛藻早出現在螺殼上。藉由淡水生物指標的分類推測：兩樣區池塘水體有機污染程度，可能介於β-中腐水性(β-mesosaprobic,βm)至α-中腐水性(α-mesosaprobic,αm)的範圍之間，而Ｄ池塘受污染的程度應會比Ｂ池塘還要更嚴重些。至於兩樣區池塘水體，已面臨了何種優養化的程度？究竟是屬於哪一個階段的污染等級呢？除須補充水體中浮游性及附著性藻類分類的詳細觀察資料外，仍必須參照部分水質化學分析法所測得的數據作佐證，才能在日後持續地研究過程中進行更精確及深入的探討。
A Study For Heavy Metals Detection Using The Solubility change of Different Kinds Solute
A research on how a solution mixed with two or more solutes affect the solubility of each solute was done. If different solutes are put in a solution with a specific concentration, the solubility of the single state solute increases, stays the same, or decreases. The difference in solubility caused by several solutes will give solutions on selectively collecting heavy metals from bodies of water polluted by these heavy metals even more effectively. To test the changes in the solubility of the solution with several solutes, sedimentation has been used; however, colorimetric analysis was also done to observe the changes in color of solutes that show color in the solution. Nitrate compounds of Sodium, Magnesium, Strontium and Potassium were used for the typical metals, and for the transition metals, nitrate compounds of Silver, Cadmium and Lead were used. Test results show that Sodium decreased the solubility of Lead, and as the concentration of Sodium increased, the solubility of Lead increased too. On the other hand, K increased the solubility of Pb, and as the concentration of K increased, the solubility of Pb decreased. We can use the radius of ions and the enthalpy of hydration to explain the changes in solubility. In the case of Cd, as the concentration of K, Mg and Sr increase, the solubility of Cd increased with it. This is because the difference between the ionic radii of Cd and the other ions (K, Mg, Sr) has a tendency to increase in solubility in the enthalpy of hydration. Also, as the concentration of Ca increased, there was a tendency in which the solubility of Cd decreased. This is because the ionic radii of Cd and Ca are similar to each other and there was a tendency that the solubility decreased in the enthalpy of hydration. Through this experiment, the study shows the tendency of the change in solubility of several solutes by comparing ionic radius and the enthalpy of hydration, and if this result is used, the processing of certain heavy metals in the bodies of water can be even more effectively used than the previous method.
Turning Prawn Pond sludge into a fertifizer
This project is a research to find out if prawn pond sludge can be used as a fertilizer . This because so far there is no beneficial use of these prawn pond sludge from over 154,000 hectares of water pond culture in Sarawak . The objectives of this project were to determine if this sludge can be combined with topsoilito produce an eco-friendly and effective fertilizer for plants and to investigate what combination of sludge and top soil is the best for plant growth . It was found that sludge has high nutrient contents such as nitrogen , phosphorus , sulphur , potassium , calcium , magnesium and iron . The effectiveness of sludge as a fertilizer was investigated by growing water spinach or “kangkong” (Ipomoea aquatica) in 5 different mixture of sludge and topsoil for 3 weeks . After the third weeks , the results showed that the combination of 30% sludge and 70% topsoil produced the best results in terms of height of plant , survival , number of leaves , stem thickness and average plant weight as compared to the other combination of 0%：100% , 50%：50% , 70%：30% and 100%：0% sludge top soil ratio . Therefore , prawn ponds sludge has a great potential to be process to become a fertilizer .
綠色陶土分子篩-污染大剋星
現今日常生活充斥著有機污染物，然而處理含有有機污染物的廢水需要極高的成本，有鑑於此，我們參考Fenton Reaction，從成本、毒性、活化能、操作方便性、二次污染及經濟效益的多方考量下，選擇以分子篩來固定鐵、鈷、鎳、錳、鋅之金屬離子，並決定以鐵分子篩為研究主軸，並探討其催化過氧化氫對有機物的分解。鑒於粉末狀的分子篩容易流失，我們以陶土固定分子篩，製作成反應杯槽，發現了分子篩與陶土的結合性。接著藉由二氧化碳感測器及光譜儀來感測有機物的分解速率，在控制溫度，濃度等條件下，探討分解有機物的反應及其反應時的特性。由實驗結果得知，分子篩能有效分解簡單醇類、氯仿、四氯化碳及indigo。使用0.35克陶土鐵分子篩，1M 以下的雙氧水50ml，其分解異丙醇所生成二氧化碳的速率可達0.34-0.55ppm/sec 之間（3.1-4.9×10-9mol/sec），此外有機氯化物分解後生成無毒性的氯離子；indigo染料分解後褪色。本實驗證實，陶土鐵分子篩：一、可以重複使用；二、可在較低濃度環境下運作；三、在酸性較弱環境下運作；四、可在低溫環境下運作(10℃)；五、不須對大量鐵離子做沉澱回收的工作（此五點優於Fenton Reaction）。相較於TAML 等人工合成的催化劑雖有避免污染的優點，但卻有無法重複使用的缺點，綜合以上幾點看來，陶土鐵分子篩在操作方便性及經濟與環境保護上具有相當的潛力及價值。With organic pollutants everywhere and the high cost to dispose of them, this study, a two-stage experiment, aimed first to evaluate the efficiency of zeolite with different metal ions and then to compare their rates in reacting to the decomposition of organic matter with hydrogen peroxide as the catalyst. Since zeolite powder can be easily washed away, we tested zeolite with clay to hold such metal ions as Fe, Co, Ni, Mn, and Zn and finally used the Argillaceous Fe-zeolite for its superiority on the basis of cost, toxin, activation energy, easy operation, and contamination. A carbon dioxide sensor and a spectrometer for visible light were used to measure the decomposition rate of organic matter under controlled temperature and resolution concentrations. The results of the experiments showed that zeolite achieved excellent effects in decomposing organic chlorides such as lower alcohols, chloroform, and carbon tetrachloride. When 0.35g of zeolite and less than 1M of hydrogen peroxide resolution were used the rate of carbon dioxide production reached 0.34-0.35 ppm/sec (3.1-4.9x10-9 mol/sec). The decomposition of organic chloride produced nontoxic Cl and the indigo dye faded after it was decomposed. Our experiments proved that Argillaceous Fe-zeolite has the following five advantages over Fenton Reaction. First, it can be reused. Second, it performed well at lower concentrations. Third, it worked well under weak acid conditions. Fourth, it worked at a lower temperature (20℃). And finally, there was no need to recycle a large amount of Fe ions. Argillaceous Fe-zeolite was also found to be superior to TAML, which has the advantage of avoiding contamination but is not reusable. The above observation and discussion demonstrate that Argillaceous Fe-zeolite possesses very significant value in terms of easy application, economy, and environmental protection.
以Geobacillus thermoleovorans T4 菌株轉化農業廢棄纖維素為葡萄糖以發展生質酒精
我們得知從高雄糖廠及堆肥中篩出的嗜高溫好氧菌Geobacillus thermoleovorans T4，是本種細菌首先被報導具有纖維素分解能力的菌株，但目前僅止於學術研究階段，尚未實際應用於廢棄纖維素的分解上。本研究以廢棄農作物纖維素取代學術研究所用的羧甲基纖維素(Carboxymethyl Cellulose，CMC) ，將T4菌株置於稻稈與米糠培養基內，進行分解效能比較，再利用酵母菌的發酵作用進一步將葡萄糖轉化為酒精。本研究發現，在60℃的環境中，活化的T4 菌株可在CMC、稻稈及米糠培養基中生長繁殖且發揮其分解纖維素為葡萄糖的能力，尤其在米糠培養基中的分解效果最快也最好。此外，將生產的葡萄糖加入酵母菌之後，初步發現也能成功地進行發酵作用產生酒精，生質酒精的產出指日可待！;The Research on Using Geobacillus thermoleovorans T4 to Turn the Deserted Cellulose in Agriculture into glucose in Order to Produce Bio-Renewable Energy It has been found that Geobacillus thermoleovorans T4, a thermophilic aerobic bacterial strain isolated from a sugar refinery wastewater （55-60℃）in Kaohsing, Taiwan, can secrete thermostable endocellulase and hydrolyze carboxymethylcellulose （CMC）in some academic research, but it is stillunknown whether T4 hydrolyzes deserted cellulose in Agriculture. The aim of this study is to investigate the best conditions of T4 cellulase activity after mixing with deserted cellulose (such as rice bran and rice straw) by measuring the glucose concentration and bacteria number, and to produce the ethanol by activated yeast. T4 was added rice bran and rice straw medium, and cultured in 60℃ for 10 hours. The number of T4 and the concentration of glucose were measured every two hours. The best conditions were examined by comparing the hydrolyzation efficiency of T4 in different cellulose medium. We observed that T4 grew efficiently in different cellulose medium and hydrolyzed cellulose into glucose, especially in rice bran medium. The yeast also converted glucose into ethanol. Our research may shed light to the development of bio-renewable energy!
無機砷與硒對染色體傷害之交互作用
中文摘要 重金屬對人體傷害的無遠弗屆-人人皆知，根據流行病學的研究指出，長期處在 重金屬含量過高之地區，易造成生理病變。比方說，長期生活在砷含量高的環境中， 易導致肝臟、周邊血管及神經系的損害，各種癌症的發生機率也大為提高。 本實驗以中國倉鼠卵巢細胞（CHO-K1）為材料，利用微核偵測技術(Michronuclei, MN)及姐妹染色分體互換(Sister chromatid exchanges, SCE)之誘引來探討亞硒酸鈉 (Sodium selenite, NaSeO2) 及亞砷酸鈉(Sodium arsenite, NaAsO2) 的交互作用 (interaction)。實驗結果顯示亞硒酸鈉本身不會造成MN 的增加，但讓SCE 頻率增加； 亞砷酸鈉會誘引增加MN 和SCE。亞硒酸鈉前處理不會減少亞砷酸鈉誘引之SCE 和 MN，但可微微降低亞砷酸鈉抑制雙核細胞(Bunucleated cells, BN)的形成，但是不明 顯。本實驗結果未能看出亞硒酸鈉前處理與亞砷酸鈉有明顯的交互作用，但發現亞 硒酸鈉會增加SCE 頻率，因此亞硒酸鈉做為保健食品值得進一步的關切及探討。 英文摘要 It is well-documented that exposure to heavy metals could cause seriously adverse effects to humans. Epidemiological evidence has shown that illness is frequently observed in residents living long-term in heavy metal contaminated area. For example, arsenic exposure was associated with increased incidences of liver diseases, peripheral vascular and neurological diseases, as well as cancers. In this report we investigated the interaction of selenite and arsenite on micronuclei (MN) and sister chromatid exchange (SCE) induction in Chinese hamster ovary-K1 (CHO-K1) cells. The present results demonstrated that sodium selenite by itself did not induce MN, but it did cause the increase of SCE frequency. Sodium arsenite significantly induced MN and SCE in CHO-K1 cells. Pretreatment of CHO-K1 cells with selenite could not reduce MN and SCE frequency induced by arsenite, but slightly prevent the cells from arsenite-induced inhibition of binucleated cell formation. In the present study, no significant interaction between sodium selenite pretreatment and sodium arsenite was observed. However, sodium selenite was unexpectedly found to enhance SCE frequency in CHO-K1 cells. The application of selenite as health protection agent warrants our concerns and further investigation.
Tharn Din-Alternative Energy Source of The Future
This scientific research project “Tharn Din - Alternative Energy Source of the Future” is directed toward producing a new type of soil charcoal. Tharn Din, with excellent combustibility. The experiments were divided into six steps. First, we selected soils of exceptional adhesion property Next, we looked into many different combustible materials for soil admixtures. Many different formulas of soils and admixtures were tested. The most promising formulas were then optimized for maximum combustibility. After which, the best formula was chosen and we further optimized its combustibility. Combustion and ignition rate and characteristics of this formula were investigated. And lastly the effect of surface area on combustible rate was examined. It was found that mud was the best adhesive and wood chip was the best admixture. Tharn Din made of one part of mud and 3 parts of wood chips released higher thermal energy than normal charcoals. We found that a special type of Thai soil, Din See-eaw, when mixed with wood chips created Tharn Din of exceptional combustibi1it Ignition and combustible rates are proportional to the surface area of Tharn Din.