全國中小學科展

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環境工程

Bio-Conversion of Agricultural Waste to Ethanol

a. Purpose of the research: Find a potential way to reduce the global warming, and develop a process for the lignocellulosic ethanol production using rice straw, which is an agricultural waste. b. Procedures: One of the greatest challenges for society in the 21st century is to meet the growing energy demand for transportation, heating and industrial processes. This significantly contributes to “Greenhouse Effect.” Rice straw is one of the lignocellulosic biomasses which are renewable organic substance and alternative source of energy. For the first time, rice straw was pretreated using autoclaving or a hypochlorite-hydrogen peroxide (Ox-B) solution, which is broadly used in potable water treatment. The pretreated rice straw was hydrolyzed with two kinds of enzymes: Cellulase and Spezyme. Following hydrolysis, Saccharomyces cerevisiae and Pichia stipitis were inoculated for ethanol production. c. Data: With the 5% Ox-B sample (initial sugar concentration was 5%), the final ethanol concentration was about 1.1%, is 87.3 % of stoichiometric and fermentation efficiency yield. Currently, we’re developing a new mutant, which can use glucose and xylose simultaneously, by using soft X-Ray. In conclusion, for the first time, rice straw was pretreated by using autoclaving or hypochlorite-hydrogen peroxide (Ox-B), solution which is broadly used in potable water treatment. The Ox-B solution treatment was an essential step for efficient hemicelluloase hydrolysis. Using 5% rice straw sugar, 1.1% ethanol was obtained. d. Conclusion: Further optimization study of fermentation process and strain improvement researches are in progress. We hope to see future cultivators to move through the energy of ethanol produced by rice straw. This project has a great emphasis towards understanding the importance of bio-energy and its nature.

『凍』人心『鹹』,『黃』金Style~以冷凍凝膠法創作速成鹹蛋黃之新『蛋』生

蛋黃酥所使用鹹蛋黃來自於鴨蛋鹽漬而成,取出鹹蛋黃剩餘之蛋白因鹽度高,無法再利用只能拋棄相當不環保,本實驗利用將蛋冷凍再解凍以分離出蛋白及蛋黃,蛋白可加工再利用,凝膠蛋黃則可鹽漬成鹹蛋黃。結果發現蛋黃在-18℃冷凍3天可完全凝膠,以30%食鹽水鹽漬40-60分鐘,其鹽度已與市售鹹蛋黃相當。在喜好性感官品評發現,鹹味、口感及整體喜好性均與傳統鹹蛋黃相似。解凍蛋白製成蛋糕在喜好性感官品評發現,色澤及口感評分最高優於新鮮蛋白,在香味及整體喜好性則與新鮮蛋白無差異。速成鹹蛋黃僅須1/10生產時間且蛋白可回收再利用並減少鹹蛋白廢棄物。

Biodiesel:does it make cents?

My project explores the practicality of biodiesel. It researches the argument of food versus fuel, compares the energy efficiencies of petroleum diesel and biodiesel and studies the effect of temperature on biodiesel. To study the effect of temperature on biodiesel, I blended biodiesel with petroleum diesel. Biodiesel blends are represented by the letter B, and the percentage of biodiesel. I used B5, B10, B20, and B50 blends, as well as pure biodiesel. I then observed the reaction of the biodiesel blends with cold weather. My pure biodiesel and B50 blends gelled to an unusable point within 15 minutes outside at -20oC. The B20 blends didn’t gel until about a temperature of -20oC. I have concluded that the B20 blend would be best for the summer, and the B10 or B5 blends would be the best for winter in northern climates, where I live. Although the B10 blends gelled slightly around -30oC, this would only be a problem for northern climates. As I predicted, more energy is produced by biodiesel than is consumed in the production process. The process of making biodiesel uses 0.31 units of energy to get 1 unit of energy out. More energy (1.2 units) is used to produce petroleum diesel than is yielded (1 unit). Although B20 isn’t as energy efficient as B100, the energy factor is still only 0.98 units of energy in for every 1 unit of energy out. When I started this project I thought that Canada would have enough farmland to produce the canola needed to run the country on biodiesel. This is only partly true. Canada would have enough farmland, but only a fraction of that land is actually used to plant canola. By my calculations, Canada has enough canola to generate enough B20 for a year. This may seem like a drawback but realistically, a higher blend would be impractical due to the gelling factor. Also, if a B20 blend was used, land would be available to grow canola for other markets. If B100 was used, there would only be enough diesel for about three months consumption. By using all of the land for biodiesel feedstock, canola would become unavailable for other markets. If canola exports ceased, the countries that depend on our canola will be in a lot of trouble. Canada already produces a lot of canola and vegetable oil. Most of it goes to the fast food industry. The United States produces over three billion gallons of fryer oil yearly. This could provide Canada with a B50 blend for a year. Utilizing used vegetable oil in making biodiesel actually reduces emissions even more. This is accomplished by using a product that would normally go to waste and decompose, producing more carbon dioxide emissions. During this project, I have found that biodiesel is a practical alternative to petroleum diesel, if it is used in a blend. My experiment proves that biodiesel is a fuel alternative that could be implemented immediately, and one that does not require the research needed for other fuel alternatives such as hydrogen or electricity.

綠色化學-應用吸水高分子螯合銀離子並製成奈米銀

聚丙烯酸(PAA)是尿布中吸水成份,我們利用其結構上的羧基螯合Ag+,並成功以甲醛將Ag+還原成奈米銀。但我們意外發現螯合Ag+的PAA白色粉末在沒有其他還原劑存在下也會漸漸變成黃色,與「利用甲醛還原製得的奈米銀」顏色十分相似。本實驗即揭開此粉末變色真相,希望能在不使用額外還原劑下,直接以PAA將螯合的Ag+製成奈米銀,不僅具環保與實用價值,更能使奈米銀製程朝向綠色化學。我們透過水溶性聚丙烯酸鈉(SPA)在均相中進行變色機制探討及影響變因的研究,找出有利的反應條件,再應用於PAA。研究證實PAA能將螯合的Ag+同步還原成奈米銀,反應機制與檸檬酸鈉作用模式相似,而提升濃度、溫度或照光有利於反應,酸性條件則不利,照各種色光也有所差異。

探討以最經濟的方式偵測低濃度溶液之金屬離子

A novel technology for detection of trace concentration of ions at ppb level was developed. The detection limit of this method can be reached to 1ppb. The Wheatstone bridge usually be used for determination the concentration of metal ion, the major disadvantage of this technique is the precision too low. Atom absorption spectroscopy is better for monitoring trace metal ion, but the cost of instrument is too expensive. In this study, the oscilloscope was used to modify the Wheatstone bridge Circuit. The difference between the voltages of two connection points of Wheatstone bridge was used to estimate the concentration of ions. The amplification system was used in this method to improve the limit to detect ion from 10 ppm to 10ppb. The parameters of measurement including types of probe, match of Wheatstone bridge resistances, parameters of input signal and electrolysis in solution were also being studied. 本研究主要是發展一個富有創新且經濟的ppb ( parts per billion ; 10-9)級,微濃度金屬離子量策技術,目前本實驗設計已可以達到 ppb的偵測極限,尤其本方法不僅能夠應用於定量,尚可做定性偵測。簡易的溶液濃度技術常使用惠司通電橋的方法,但這個方法的缺點在於精確度較低,而另外常用的儀器是原子吸收光譜儀,卻價格高昂。因此,我們回頭對惠司通電橋的技術作改良和創新技術。首先,最重要的新技術是對示波器的改良。示波器被用來量測惠司通電橋的電位,我們比較電橋上兩特定點的電位來判斷濃度,當兩個點的電位相同時代表溶液電阻和控制電阻相同。但當輸入信號的電壓太強時,示波器上並無法判別細微的電位變化,因此造成量測的精度不高。本組設計新的電路用以放大在高強度訊號的波形細微變化,成功將測量極限由 10 ppm推進到 ppb。另外關於此設計中的各種參數,包括探針類型、惠司通電路的電阻匹配、輸入訊號之強度頻率、電極間電容效應及溶液中可能發生的電解效應等,都有精確的探討。最後的設計結果將是創新且有別於以往的設計概念。

Sisa-Friend or Foe?

It is beyond my logistical ability to determine the extent of the sisal problem in Namibia and as such I have investigated the following aspects, which should be of value to countries such as Tanzania and Kenya where there are still large plantations of the sisal plant. There is also a need to establish industries in Namibia and it is for these reasons that plant has been investigated.

七星映月--都市靜水生態系連接度之模擬研究

目的:1. 以地理資訊系統分析臺北市靜水生態系的分布狀況;2. 分析靜水生態系的連接度。過程和結果:我實地踏察了臺北市區的381 個地點,在其中187 處發現224 個靜水生態系,首次統整出臺北市區靜水生態系的現況資料。靜水生態系的平均密度為0.82 個/km2,其密度與人口密度成正比。以 GIS 軟體標定這些水池,發現它們成任意或叢生分布。再以 VisualBasic 程式語言設計程式,以預測移動能力不同的濕地生物在這些靜水生態系間移動的情形。結論:由程式模擬可知,能在所紀錄地點間自由移動的濕地生物種類很少,顯示都市化對靜水生態系的隔絕效應。In a city, still water ecosystems (ponds and lakes) are divided by buildings and roads, so they are not continuous in the space. I surveyed 381 sites in Taipei City and found 224 ponds and lakes at 187 sites. The density of still water ecosystems is 0.82 per square kilometer. The density of still water ecosystems is higher in areas where more people live. I use a geographical information system software to mark the locations of these ponds. Their distribution is either random or clumped. I use Visual Basic to design a program to predict how do wetland creatures move among these sites. My program tells that very few creatures can move freely, indicating still water ecosystems in this city are quite isolated.

Development of a bio-monitoring system using behavioral pattern recognition of the medaka(Oryzias la

Water pollution is a severe problem for human beings. Sewage and hot water\r coming from homes and factories have changed the environment for aquatic\r animals. To manage a polluted environment, an accurately designed monitoring\r system is needed that will detect signs of toxic material or increase in temperature as\r fast as possible. A new and more accurate bio-monitoring system, which involves\r actual creatures, needs to be developed to save the aquatic environment from\r pollution.\r This experiment focused on discovering a new way to monitor aquatic\r creatures by observing the behavioral patterns of the medaka and observing how\r they changed when exposed to external stress, especially an increase in temperature\r or exposure to insecticide. The behavioral patterns of the medaka were observed and\r measured by a tracking program called the Movement Viewer. In the transition\r period, that is, when the temperature was raised from 25°C to 35°C, the total moving\r distance of the medaka suddenly increased compared to that in 25°C. When the\r medaka was in a temperature of 35°C, it slowly adapted to the new environment and\r the total moving distance showed a similar value to that in 25°C. For section\r dominance, the medaka showed a sudden increase in the dominance of the top part\r of the tank, meaning that the medaka tended to swim on the surface of the\r experimental aquarium. Again, it slowly adapted when the temperature had\r completely changed to 35°C.

SEAWEED ON THE NAMIBIA COAST EXTRACTION OF AGAR & USE OF KELP AS FERTILIZER

In Swakopmund we have a problem with seaweed deposits on our beaches. Tons of seaweed are throwni away yearly. The reason I did this project was to see if the seaweed on the Namibian coast can he used instead of just being thrown away.Seaweed is a rich source of natural minerals and vitamins such as calcium, potassium iodine and also Vitamin A, BI and K. The seaweed on our coastline cannot he eaten as the salt content is too high. Seaweed is a marine algae that works like a sponge absorbing nutrients and minerals from the water.I extracted agar (a jelly) from red seaweed called(GracilariaVerrucosa). I dried the agar and seaweed, and I also grew mushroom spawns and bacteria on the agar plates. Secondly, I researched the possibility of using kelp (Laminaria Pallida), as fertilizer and livestock supplements. The unemployment rate in Namibia is very high. With adequate financial resources and proper marketing, seaweed can he used to increase the economy. We can market the agar to pharmaceutical and cosmetic companies, and also supply it to the university for students in Biology to grow bacteria and other micro organisms. The ferti lizer can be used to boost agriculture in Namibia. It is cheap and the farmers will he able to p1 ant vegetables with better nutritional value.All this was done to promote of biggest natural resource to create more work and to find a way for the less fortunate to create a better life for themselves.

金奈米粒子的合成與其光譜特性

當世界各國正全力朝奈米科技發展,而奈米微粒對環境及人類健康影響與否未明朗之際,有關奈米微粒對人類健康影響之探討是刻不容緩之事。本研究的近程目標是合成金奈米粒子,遠程目標是擬探討金奈米粒子的生物毒性。此份研究主要是描述金奈米粒子的最大表面電漿吸收光譜特性及其最大吸收波長,而金奈米粒子的合成是利用四氯金酸( HAuCl4 )與還原劑—硼氫化鈉( NaBH4 )、聯胺水合物( N2H5OH )或檸檬酸鈉在陽離子界面活性劑的溶液( CH 3(CH2)15N+(CH3)3Br– ,溴化十六烷基三甲基銨,CTAB ) 當保護劑中,利用氧化還原方法直接生成金奈米粒子,並探討四氯金酸的濃度、還原劑的濃度、界面活性劑的濃度及反應溫度,對金奈米粒子生成之影響,另外也探討金奈米粒子的穩定性。有關金奈米粒子的光譜特性則利用UV/Vis 光譜儀測試。研究結果發現在相同的條件下,利用冰浴,以NaBH4 當還原劑所合成出的金奈米粒子之平均粒徑為11.70 nm ,而以N2H5OH 當還原劑所合成出的金奈米粒子之平均粒徑為20.25 nm, 有關金奈米粒子之結構及大小藉由穿透式電子顯微鏡( TEM ) 拍攝出的影像得以驗證。;This study mainly focused on the properties of the maximum surface plasmon absorption of gold nanoparticles and their wavelengths. The synthesis of gold nanoparticles made use of HAuCl4 aqueous solution and reducing agent, through the oxidation reduction method, with reductant, sodium borohydride ( NaBH4 ), hydrazine ( N2H5OH ) or sodium citrate solution in the presence of the cetytrimethylammonium bomide ( C19H42BrN,CTAB ) aqueous solution as protecting agent. The influence of concentration of HAuCl4, reductant, surfactant, and temperature on the synthesis of gold nanoparticles were explored. In addition, the stability of the gold nanoparticles was also explored. Measurements of spectral properties of gold nanoparticles were done by UV/Vis spectrometer.When NaBH4 was used as a reductant, the average diameter of gold nanoparticles was 11.70 nm. When N2H5OH was used as a reductant, the average diameter of the synthesized gold nanoparticles was 20.25 nm. The structures and sizes could be identified by the transmission of electron micrograph (TEM) images.