植物學

本研究以結構穩定、能溶於水，並會影響人體生長發育的固醇類激素──副腎上腺皮質激素作為探討對象，嘗試了解此類之類固醇類激素對植物的生長發育是否有影響。結果發現無論是雙子葉的豆科植物或是單子葉的禾本科植物，其根、莖、葉的生長皆會受到激素的抑制，且出現激素濃度越高，抑制情形越顯著的情形。此外，統計學的結果顯示，類固醇會蓄積在植物體內，造成累積性的抑制作用，此現象和動物使用過量類固醇類激素時產生之副作用十分相似。 The present study investigates the effect of cortical sterile on the growth of plants. The results show that it has effect on both dicotyledons and monocotyledons in the roots, stems and leaves. It is also shown that the higher the concentration, the more marked suppression of their growth. Statistical analyses suggest a cumulative effect, which has also been found of overdoes of cortical sterile on animals.

Previously, I studied the acute effects of a common broadleaf herbicide, Killex (by Montsano) and a pesticide, Spidercide (by Wilson), on cytoplasmic streaming in detached leaves from the pond weed, Elodea canadensis. I found that Killex, active ingredient 2-4D, an auxin analog, applied at the recommended dose decreased the rate of cytoplasmic streaming by 64% over a 20 minute observational period. The Spidercide, active ingredient d-trans allethrin, a Na+ channel activator, stopped cytoplasmic streaming after a 5 minute exposure and no recovery was observed during the 20 minute observational period.

Few plants can grow in the place where Wedelia trilobata grows. In the following experiment, the leaves ans stalks(of the plants from Wedelia trilobata) are extracted for experimental purpose .The data and records of the experiment have shown that Wedelia trilo ata indeed refain the growth of plants such as mung bean, teeding seed, yeast’s respiration and the photosynthesis. We use the function of dieing the polyphenols to know that the Wedelia trilobata can produce Allelochemicals called as the polyphemols The mesophycell produce the polyphemols from chloroplast of cortex,the polyphemols transport to root and release from root exudation. The plant will absorb polyphemols of the soil, and will affect the growth of the plant. If we can separate and purify the Allelochemicals, we can make it as natural herbicideb. 南美蟛蜞菊所在之處，少有其他植物，本實驗採南美蟛蜞菊植栽區之土壤與南美蟛蜞菊莖或葉之萃取液進行相剋作用之研究，結果顯示南美蟛蜞菊確實會抑制小麥與綠豆種子萌發及幼苗生長，並抑制酵母菌的呼吸作用及植物光合作用中之光反應。 利用多酚類染色方法得知南美蟛蜞菊產生的相剋化合物為多酚類，且該多酚類由葉肉細胞與莖內皮層中具葉綠體之細胞產生，經韌皮部輸送至根，再由根經泌濾作用釋出。土壤中的多酚類會被植物吸收，進而影響植物的生長與發育。 若能將該相剋化合物---多酚類分離與純化，或可作為具選擇性之天然除草劑。

我一直在想，為什麼植物的美麗不能永恆呢？美麗的花朵，伴隨答淡雅的香氣，往往能美化環境，更能淨化人心，自小生長在鄉間的我，更能感受到這份來自花草的神奇力量，因此深深愛上這花草世界．但為何花朵的美麗總是短暫，為了想讓植物開花的時間長久，於是，我著手進行在自然科技雜誌及網路上搜尋相關的資料及訊息，但是找到的大多都是使生長速率變快的報告，卻找不到使生長速率變慢的報告。終於，曾經有人運用圓周運動的原理，而成功的使動物的生長速率變慢，於是，我針對直線運動及圓周運動分別依速度快及速度慢來分成四組，以及一組靜止狀態的綠豆，將五組實驗後的數據相互作比較，結果發現，除了陽光、水、空氣、土壤、養分及本身具有的胚胎外，在不同的運動狀態下也會影響到植物的生長速率 ·I was thinking about the reason why the beauty of plants couldn’t last forever. The beauty of flowers that accompanied with sweet-smelling could beautify the landscapes and purify our heart. I felt strongly about the magic power of the plant because I lived in the country in my childhood. Therefore, I loved plants deeply. Why was the beauty of flowers transient? As a result. I started to search for the related data and information on science and technology magazines and Internet. Most of the reports increase the growth rate of plants, but I cou1dn find the reports about slowing down the growth rate.At last, someone had successfully used the principle of circumference movement to slow down the growth rate of animals. Therefore, I focused on linear and circumference movement and divided them into four groups and one group of green beans with still state. I compared the data of five groups, and found that different movement affected the growth rate of plants m addition to sun, water, air, soil, and embryo.

阿拉伯芥中，生物時鐘 (circadian clock) 會產生 24 小時之概日韻律 (circadian rhythm) 使植物適應環境中的溫度與光線變化。在阿拉伯芥中目前已經發現 LIGHT-REGULATED WD1 (LWD1) 和 LWD2 基因和調控生物時鐘有關，使其維持正常的一天 24 小時週期。當 LWD1 與LWD2 基因產生突變時，植物的生物時鐘週期會由一天24小時變為一天只有18小時。之前研究結果顯示 LWD1/2 會在維管束大量表現，由於目前對於維管束生物時鐘之運轉機制所知甚少，因此我們希望能進一步研究 LWD1/2 與維管束生物時鐘間的關係。 為了解 LWD1/2 在維管束中表現是否與其功能相關，我們將此兩種基因接上只會在維管束或葉肉細胞中表現的啟動子，轉殖入 lwd1 lwd2 雙突變株中進行互補試驗 (complementation test)；藉由觀察生物時鐘週期是否從 18 小時恢復為 24 小時，以了解這兩個基因的作用位置及功能。由實驗結果來看，我們發現不論是單獨在維管束或是葉肉細胞中表現 LWD1 或 LWD2 基因，皆會影響到阿拉伯芥的生物時鐘，整棵植株表現出的綜合週期，介於野生型 24 小時與 lwd1 lwd2 雙突變株 18 小時之間。這個結果顯示，不同組織之間的生物時鐘可能不會互相影響；而 LWD1/2 雖於維管束中有較高的表現量，但仍需要在葉肉細胞表現才能具備完整功能。

此項研究是用溶氧計連續偵測培養液中之溶氧量，藉以分析固氮\r 單胞藻RF-1 的光合韻律。實驗的結果清楚地看出RF-1 之光合韻律，\r 而且能夠清晰地觀察到其中光合作用、呼吸作用和固氮作用之間的相\r 互關係。研究中還發現：在不進行光合作用時，RF-1 的固氮作用也\r 會停止；另一方面，在有進行光合作用、但是停止固氮作用的狀況下，\r RF-1 光合作用之韻律現象會喪失。這些實驗結果值得未來更深入地\r 探討。報告中也包括了單胞藻PCC6803 的光合韻律實驗，藉以做為\r 有固氮能力與沒有固氮能力藍綠藻之間的對照。The variation of dissolved oxygen (DO) in the culture of the\r unicellular nitrogen-fixing cyanobacterium Synechococcus RF-1\r under diurnal light/dark condition was detected automatically by DO\r meter. The results indicated that the algae exhibited circadian\r photosynthetic rhythm. The results also revealed that a respiration\r rhythm occurred at dark phase when the culture was grown in\r nitrogen-free medium. There was correlation between the rhythm of\r photosynthesis and nitrogen fixation. The nitrogen-fixing activity\r could not be detected if the photosynthesis was stop by cultivated\r under continuous darkness. On the other hand, the rhythm of\r photosynthesis could not persist when the nitrogen-fixing activity\r was inhibited by NaNO3. In order to compare the difference\r between Synechococcus RF-1 and the cyanobacterium, which\r cannot fix nitrogen, the DO pattern of Synechocystis PCC6803 was\r also investigated.

在模式植物──阿拉伯芥（Arabidopsis thaliana）中，AtYAK1（Arabidopsis thaliana Yak1-related protein kinase）是目前發現唯一屬於DYRK（Dual specificity Yak1-Related protein Kinase）的蛋白激?。雖然之前研究已證明，不同物種之DYRKs 和細胞的生長與發育過程有關。然而，其在植物中的生理功能卻尚未被明確地研究報導過。在先前的研究中，為瞭解AtYAK1 在阿拉伯芥內作用之位置，前人選取AtYAK1 基因ATG 上游約2.5 kb 的序列（Upstream Element, 2.5KUSE）建構至一含有GUS（β-glucuronidase）報告基因的質體中，並轉形至阿拉伯芥，進行GUS 組織染色分析。但在初步結果中，並沒有在轉殖株觀察到明顯的GUS 表現。進一步分析，我們發現在2.5KUSE 序列末端約0.5 kb 的5’非轉譯區（5’untranslated region, 5’UTR）中，有四組開放讀序框（Upstream Open Reading Frame, uORF）。有趣的是，許多研究也顯示，uORFs 會影響轉譯過程中的再起始（re-initiation）作用而調控該基因的表現。另一方面，前人亦透過構築好的2KUSE 轉殖株（即不含有5’UTR）進行上述GUS 實驗。結果發現，此2KUSE 轉殖株的GUS 表現非常顯著。本實驗即要瞭解AtYAK1 的uORFs 是否也會影響其蛋白質的合成。首先，我們以點突變的方式將四組uORFs 中之ATG 換成TTG，目的為構築不含有uORFs 之5’UTR（mutated uORFs, ΔuORFs）。在進行原生質體短暫表現分析法（protoplast transient assay）及GUS 組織染色分析後，將結果與含有uORFs 的結果作比較：當缺乏uORFs 後，其3’端報告基因的表現量確實比原來顯著。綜合以上，我們認為此uORFs 對於AtYAK1 蛋白質之表現佔有相當重要的影響地位。最後，我們對5’非轉譯區是否存在開放讀序框進行阿拉伯芥全基因組分析，相關結果亦於本研究報告中分析討論。AtYAK1（Arabidopsis thaliana Yak1-related protein kinase）is the first DYRK（Dual specificity Yak1-Related protein Kinase ） family member identified in the model plant ─ Arabidopsis thaliana and exists as one copy gene in Arabidopsis. Previous studies showed that many eukaryotic DYRKs are involved in regulating the growth and development of cells. However, the study of AtYAK1 in Arabidopsis is lacking to date. In order to understand where AtYAK1 expresses and functions in plants, a 2.5 kb fragment which is located upstream from the major ATG of AtYAK1（termed Upstream Element, 2.5KUSE）was previously constructed to drive the expression of a reporter gene, GUS（β-glucuronidase）, in transgenic Arabidopsis. Much to our surprise, no GUS expression signal could be detected in such transgenic plants. When further analyses were performed, we found that there are four upstream open reading frames （uORFs） in the 5’untranslated region ( 5’UTR ) within the 2.5KUSE. Many studies indicating that the uORFs can regulate the translation of downstream ORF encoding the major gene product through the procedure of translation re-initiation. This action represents a mode of translational regulation for gene expression. Indeed, GUS activity could be readily detected in transgenic plants expression 2KUSE::GUS, a construct lacking the 5’UTR of AtYAK1. In this study, I have tried to elucidate whether the uORFs of AtYAK1 can regulate the translation of the downstream major ORF. First, in order to construct a 5’UTR fragment of which uORFs have been mutated（ΔuORFs）, we apply site-directed mutagenesis to substitute ATG with TTG for the four uORFs and examine the expression of GUS driven by this mutated 2.5KUSE. After analyzing the results in both Arabidopsis protoplast transient assay and transgenic Arabidopsis, stronger expression of reporter genes in both systems were observed when the four uORFs were mutated. We have also confirmed that, in transient expression system, the increase of reporter gene activity was not due to the excess accumulation of the corresponding mRNAs. Rather, it is the four uORFs which play an important role in negatively regulating the translation of AtYAK1, possibly via inhibiting the translation re-initiation of major ORF. A genome-wide examination of uORFs in all Arabidopsis genes was also performed to assess the possible contribution of uORF in regulating gene expression.

本研究針對台灣產水生植物，蓴菜之構造與生長環境、蓴菜對腸胃道常見致病細菌之抑菌效果以及主要成分暨化合物分析。由本研究結果得知，崙埤湖內之稀有浮葉型水生植物蓴菜，其生長環境為無汙染之乾淨偏酸性水源，最適合生長之生深為50-160 ㎝；水溫則為22-25℃；而蓴菜之地下根莖對表皮金黃葡萄球菌（Staphylococcus aureus）具有輕度之抑菌效果，經由分離純化得知為BS-1：沒食子酸（Gallic acid）；另外，由蓴菜之葉片分離出十種成分分別為BS-2 （Kaempferol-7-O-Glucosids）、BS-3 （Quercetin-7-O-glucosids）、BS-4（5,8,4’-Trihydroxyflavone-7-O-glucosids）、BS-5 （3,5,8,3’4’-Pentahydroxy flavone）、BS-6（Vitamin E: d-Tocopherol）、BS-7 （Glyceride）、BS-8 （Phenolic A）、BS-9（Quercetin）、BS-10（Kaempferol）、BS-11（Phenolic B）。其中發現BS-8 對神經膠腫瘤細胞株有18.42%之抑癌效果，另外，BS-2、BS-3、BS-5、BS-10、BS-11 等成分，呈現良好之美白作用。This investigation is to analyze Brasenia schreberi Gmel., a native rare floating water plant in Taiwan, focusing on the plant’ s structure, its growth environment and, most importantly, the effect of chemical compounds it produces on restraining the common pathogenic bacteria in human stomach. The result indicates that the most suitable growth environment for Brasenia schreberi Gmel. is in slightly acid, pollution-free water such as that in the lake Lung Pi in northern Taiwan. The ideal water depth for its growth is 50-160 cm, and the water temperature is 22-25°C. The impractical BS-1 (Gallic acid) extracted from the izome of Brasenia schreberi Gmel. by separation and purification has a light effect on restraining Staphylococcus aureus, a bacteria in the stomach. From the epidermis of the blade of Brasenia schreberi Gmel., ten other ingredients are also isolated, including BS-2 (Kaempferol-7-O-glucosids), BS-3 (Quercetin-7-O-glucosids), BS-4 (5,8,4’-Trihydroxyflavone-7-O-glucosids), BS-5 (3,5,8,3’,4’-Pentahydroxyflavone), BS-6 (Vitamin E: d-Tocopherol ), BS-7 ( Glyceride ), BS-8 (Phenolic A ), BS-9 (Quercetin), BS-10 (Kaempferol),and BS-11 (Phenolic B). BS-8 is found to resist cancer C6 ( Glioma ) by 18.42%, while BS-2,BS-3, BS-5, BS-10, and BS-11 show an outstanding effect on skin-whitening.

在「植物的秘密生命」這本書中提到植物在電場或磁場中會生長的更好，我最近正好學到了電磁場如何產生，以及一些產生電磁場的方法，所以本研究即利用螺形管線圈來產生均勻的電磁場，並用運用安培右手定則來控制磁場產生的方向，來探討電磁場在不同條件下對實驗植物－綠豆生長的影響。本實驗使用銅質線圈纏繞鐵碗的方式，且通入不同大小電流來改變磁場的強度，以探討對實驗植物－綠豆生長的影響。本研究所得到的結果顯示：(1)綠豆在通入電流50mA 時所產生的電磁場可促使綠豆達到最好的生長效果；(2)栽培綠豆環境中所架設的電磁場較高，可使綠豆表現出較為明顯的生長速率；(3)促進綠豆的生長並不需要二十四小時都通入電流；(4)綠豆在N 極朝上的電磁場中生長的速率較S 極朝上的磁場中要快；(5)在較低溫度環境中，電磁場促進綠豆生長的效應較為顯著。;It has been mentioned that the plants will grow faster in the electronic or magnetic field in the book of “the secret life of plants”, I learned about the knowledge and methods of how to produce the electromagnetic field just now, I use the screw wire to produce electromagnetic field and control the direction by the Amplifier’s rule to investigate the growing speed of green beans under electromagnetic field in those different temperature. In this experiment, I use the wire to screwed around the ironed bowl, and make different electric current to generate different strength of electromagnetic field, the results indicate that (1) The faster growing speed will only exist in some strength of electromagnetic field, (2) The higher ironed bowls that full of screwed wire will result in the faster growing speed, (3) It will not need electromagnetic field all days, (4) The green beans will grow fast in those north magnetic pole than those in south magnetic pole, (5)The effect of electromagnetic field to increase growing speed will be significant in higher room temperature.