全國中小學科展

由溶氧量之變化分析單胞固氮藍綠藻光合韻律之特性

科展類別

臺灣國際科展

屆次

2003年

科別

植物學

學校名稱

國立高雄師範大學附屬高級中學

指導老師

陳桂芳、蕭淑娟

作者

嚴婉禎

關鍵字

溶氧量,單胞固氮藍綠藻

摘要或動機

此項研究是用溶氧計連續偵測培養液中之溶氧量,藉以分析固氮\r
單胞藻RF-1 的光合韻律。實驗的結果清楚地看出RF-1 之光合韻律,\r
而且能夠清晰地觀察到其中光合作用、呼吸作用和固氮作用之間的相\r
互關係。研究中還發現:在不進行光合作用時,RF-1 的固氮作用也\r
會停止;另一方面,在有進行光合作用、但是停止固氮作用的狀況下,\r
RF-1 光合作用之韻律現象會喪失。這些實驗結果值得未來更深入地\r
探討。報告中也包括了單胞藻PCC6803 的光合韻律實驗,藉以做為\r
有固氮能力與沒有固氮能力藍綠藻之間的對照。The variation of dissolved oxygen (DO) in the culture of the\r
unicellular nitrogen-fixing cyanobacterium Synechococcus RF-1\r
under diurnal light/dark condition was detected automatically by DO\r
meter. The results indicated that the algae exhibited circadian\r
photosynthetic rhythm. The results also revealed that a respiration\r
rhythm occurred at dark phase when the culture was grown in\r
nitrogen-free medium. There was correlation between the rhythm of\r
photosynthesis and nitrogen fixation. The nitrogen-fixing activity\r
could not be detected if the photosynthesis was stop by cultivated\r
under continuous darkness. On the other hand, the rhythm of\r
photosynthesis could not persist when the nitrogen-fixing activity\r
was inhibited by NaNO3. In order to compare the difference\r
between Synechococcus RF-1 and the cyanobacterium, which\r
cannot fix nitrogen, the DO pattern of Synechocystis PCC6803 was\r
also investigated.

由溶氧量之變化分析單胞固氮藍綠藻光合韻律之特性

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