全國中小學科展

2013年

筆筒樹凋萎病防治法 -病原Ophiodiaporthe cyathease sp. nov.拮抗菌之篩選與應用

近在台灣新發現的筆筒樹萎凋病,造成大量筆筒樹(Cyathea lepifera)枯萎死亡,對於此疾病的防治已成亟需解決的問題。先前的研究中,已確認病原菌為新屬真菌並命名為Ophiodiaporthe cyatheae sp. nov.。本研究則在於以生物交互作用方式進行防治,尋找拮抗菌,針對特性實際應用。 研究中因實驗素材與操作方法的差異分為細菌與真菌類拮抗菌兩大主軸,且皆成功自土壤中各分離出數種有應用潛力的拮抗菌。細菌方面,尋獲並經鑑定為Burkholderia gladioli,對於病原菌有良好抑制效果,然由於此菌本身安全性有疑慮,實用上仍有待商榷。真菌方面,拮抗菌以超寄生方式與快速的生長能力制伏了病原菌,期待透過比較消除病原菌的速度,篩選出其中最強大適切的拮抗菌種,運用於實際田間病殘株處理。

Equipping, programming and testing a robot searching for an avalanche transceiver

1. Purpose of the research Because we live in a famous winter sport region in Switzerland, we have been confronted with the problematic of avalanches since we were born. In winter 2011/2012 alone, 179 people were involved in an avalanche accident, of whom 25 died. The most important device for searching and rescuing a buried person is the avalanche transceiver. It creates an electromagnetic field, which can be used to locate a buried person with another transceiver. The most important factor while searching is time: After 30 min. the chance of survival of a buried person has dropped to 40%. Considering the fact that people often make mistakes when they are put under such a big stress, valuable time gets lost. This is where our project comes into play. With an automation of the searching process a fast and reliable search should be achieved. The aim we agreed on for this paper is to develop a prototype of a robot that can find an avalanche transceiver automatically and reliably. To study and optimize the functionality of our robot more easily, our tests have only been performed on flat ground. 2. Procedures The components of an avalanche transceiver with only one transmitting antenna are used as a receiver. With the help of this receiver, the signal of a transmitting avalanche transceiver was analysed. Furthermore, the results were used to evaluate the most suitable search technique. Our robot is based on the “RP6” robot system, which is programmable in the C-language. The signal of the receiver is modified in a way which allows the robot to read it. Based on the results of the signal analysis, a search algorithm is developed. In the final tests the robot was examined as to its functionality and efficiency. Therefore, the robot was positioned at randomly chosen starting points to locate the transmitting device. 3. Data The transmitter could be located correctly in every test. The starting points were successfully located up to 11 m from the transmitter. The search ending points were found at a maximum range of 0.5 m from the correct position. 4. Conclusions In view of the results, the aim of developing an automatic localisation of an avalanche transceiver on flat ground as a prototype is seen as fulfilled. Outside the specified range, the transmitter can only be located unreliably. This is due to the fact that outside that range the signal differential in different directions is too small to be processed by the robot. Our prototype was tested under ideal conditions. Certainly, our robot is still some way from being ready for use in an actual avalanche. Many different questions have not been answered yet or have come up during this project, for example: ‘How should the robot should be applied on uneven terrain?’ or: ‘How should it deal with several buried people at once (multiple transmitted signals)?’ Two aspects of our prototype, the low weight and the low construction costs, however, are particularly favourable.

Building Bridges with Water-The Floating Waterbridge

This paper describes the investigation of a fascinating physical phenomenon called the “floating water bridge”. Despite the fact that water is undoubtedly the most important chemical substance on earth, it is practically ubiquitous and it still represents one of the best explored substances, still not all characteristics are well-understood. There are some phenomena like the “floating water bridge”, which cannot be explained. If high voltage is applied to two beakers, which are arranged close to each other and which are filled with deionized water, a connection forms spontaneously, giving the impression of a floating water bridge. For the experiment discussed in this paper, two beakers with a diameter of 50 mm and a height of 80 mm are filled with triply deionized water. Platinum electrodes are submerged in the center of the beakers, one set to ground potential (anode), the other one on high voltage, up to 25 kV dc. Within the scope of this work, an experimental setup was developed, which enables measuring and demonstrating the most important parameters like voltage, current, length and temperature of the water bridge as well as the mass transfer between the beakers. In addition the correlation between the different parameters and the influence on the water bridge could be estimated. Once the beakers are separated, the bridge remains stable for several hours up to a length of 2.5 cm. With platinum electrodes and no electrolysis observed, a small current (≈300µA), a mass flow from anode to cathode and forces were measured. Pictures, taken with an infrared camera and a new developed method to record "infrared-videos", enabled to visualize the heat flow in the water bridge. Furthermore the conversion of energy and the dependence of charge and mass transfer could be estimated roughly. In the course of the investigations it was also tried to prove the water bridge with other liquids like castor oil, olive oil, a mixture of glycol and water as well as tap water - for some of them for the first time. Supplementary the experimental setup was varied by using different electrodes with different sizes and different material as well as beakers of different sizes and materials. In addition, a qualitative explanation was developed. The results of this work enable a better understanding of the floating water bridge and provide a basis for further research as well as for development of future practical applications. One of these applications could be an improved waste water treatment process.

澱粉吸附蛋白質與澱粉糖的分子交互作用

顆粒澱粉可和碘形成藍色錯合物(starch-iodine complex),我從種子發芽澱粉酶實驗了解澱粉酶(amylase)具糖解酵素活性,會分解澱粉盤上的澱粉,並使澱粉失去與碘形成螺旋狀澱粉-碘錯合物的能力,讓藍色澱粉盤出現透明水解圈。通常澱粉酶之分子結構包含澱粉吸附區及催化區,分別負責吸附大分子或不可溶性澱粉糖(amylose)配體及分解澱粉糖成可溶性小分子糖。本計劃選用分子量、溶解度不同的澱粉糖分別與碘液反應,再加入澱粉酶(glucoamylase)或其澱粉吸附區(starch binding domain, SBD),發現二者均會破壞澱粉糖與碘結合之螺旋狀結構。分析二者之反應效率,可比較不同蛋白質解開澱粉-碘錯合物之能力。此外,本研究亦比較正常與突變株二聚體澱粉吸附區(SBD dimer)之解開澱粉-碘錯合物反應活性,再查詢文獻、比對不同蛋白質的序列、結構、及功能特性進行綜合分析。根據本研究結果,澱粉吸附蛋白質序列中解開配體螺旋結構最重要的官能基為兩個具有苯環的胺基酸,且二者對於可溶性及不可溶性配體之影響不同。本研究提供科學家了解自然界具備重複澱粉吸附區(tandem repeat)功能的邏輯方法,未來可應用於澱粉代謝、合成相關之蛋白質設計、結構預測、及功能分析。.

具備節能協定之智慧型開關裝置的設計與研究

本研究參考智慧型3C產品之節能協定,將其應用於電源開關裝置上,此開關裝置將具備工作模式、待機模式及睡眠模式,利用此三種模式並結合即時輪詢的技術,發展出適合智慧型節能開關裝置的節能協定,達到全自動且節能的目標。此智慧型節能開關裝置主要包括固態開關電路、電流感測電路、RC濾波器電路以及控制電路,本研究針對上述幾種主要電路進行電路的研究及實作,並建立模擬的電路模型,根據此模型設計出完整的智慧型節能開關裝置,進行其節能效率及未來大量應用的可行性分析。經過實作證明,此種具備節能協定的智慧型節能開關的確能有效地省下家電待機耗電,與市面上不具備節能協定的開關相比,有更高的使用便利性。

Microbial diversity in the Mediterranean hypersaline deep-sea lake Tyro

1. Purpose of the research Characterization of bacterial and viral diversity of brine Tyro using molecular methods of identification. 2. Procedures For bacteria: 1. Amplification or multiplication of 16S rRNA gene (one of the most conservative gene) by polymerase chain reaction (PCR). 2. Agarose gel electrophoresis and purification of PCR product 3. Ligation of purified PCR product into the vector pGEM-T 4. Transformation of plasmids containing an insert into competent cells E.coli XB1 5. Blue-white selection (we need white colonies, they contain the insert of interest) 6. Isolation of plasmids containing an insert of interest 7. Sequencing of inserts 8. Bioinformatics analysis: matching homologues from GeneBank database, construction of phylogenetic trees, statistical analysis. For viruses: The same methods were used for gp23 gene, which code a major head protein of T-even bacteriophages; to amplify gp23 gene a special set of primers was used, along with a standard cloning protocol described above. 3. Data 1) Two libraries of clones were obtained during analysis: lake Tyro (24 operational taxonomic units (OTUs), 10 classes) and sea water (6 OTUs, 2 classes of eubacteria). 2) The most abundant classes were: gamma-, delta-, epsilon- Proteobacteria, which is in agreement with previous reports about bacteria in brines of Mediterranean Sea. 3) The number of clones was not sufficient to obtain stable estimates of diversity, the analysis require additional data. 4) The diversity of bacteria was unexpectedly high in brine but not in the seawater, due to higher and more diverse ion composition. 5) Most of the detected bacteria in the deep-sea lake belonged to the previously undescribed (18,75%) bacteria or had unusual metabolism (43,75%). 4. Conclusions The analysis demonstrated unexpectedly high diversity of halophilic bacteria inhabiting Tyro lake. Most of bacteria presented in brine water had unique and uncommon characteristics based on information about its closest relatives. Therefore, the deep-sea hypersaline lakes of Mediterranean Sea have great potential for further investigations. Preliminary results of diversity of viruses of Tyro lake were obtained during analysis, more complete description is coming soon.

On Course Line Management

The Online Course Management system was developed in 2012 by George Moon to address the issue of creating course books at Burnside High School in Christchurch, New Zealand. The course books are designed to inform students, staff and parents of the many courses that are available for students to choose for their next year of study. In the past, the system that the school used consisted of large amounts of paperwork and duplication. Not only did this system require a lot of effort from staff, but the course book cost the school thousands of dollars to produce, as it had to be sent off to be published into a large book that would be read by students for a week, then likely thrown out. This year the school decided to digitise the course book, so that students would look at their courses online. Earlier this year, the school believed that the new School Management System (SMS) ‘KAMAR’ would be able to handle all of the necessary information, however this was not the case. Because of this, they needed a simple solution that would collate all of the course data, and then output it as a course book. I developed my project to do this. It is a web based program that is accessible by staff on their computers which enables them to enter in all of the course and assessment data for their departments. As it is all securely stored on a central database, it reduces duplication and staff workload, as well as the added environmental bonus of less paper being used. The program also outputs data in a number of ways including as a coursebook PDF (digital document which can be uploaded or printed), an Excel spreadsheet and a webpage for easy viewing. It can be sorted or printed by different categories (such as level, faculty, department), which proved to be a very useful feature. Following some research on areas such as design principles, browser compatibility and screen resolution (computer screen size), the program was designed to make best use of this this information. For example, most of the computers that staff would access the website on were of a similar size screen, so I made sure that my website worked well for them. I also used my research on design principles to try and create a simple, clean interface that users with limited computer skills would easily be able to navigate around. The outcome was real, as it was used by the school to generate their coursebook this year. Following a 95% student completion rate of course selection many months earlier than previous years, the system (although it had some issues) was pronounced a success, and the school is looking to use it in the years to come. There are a number of steps I am looking to take in the future with this program including the potential sale to other schools, so they can take advantage of the features it has to offer.

USING IRON-STEEL INDUSTRIAL WASTE SLAGS AS A FERTILIZER

Steel slag, is received from iron and steel production facilities in Turkey, and is up to 25-30% from steel production. These couldn’t have been reclamation for any field of use and free stored in nature. When the structure of this slag examined, it was contained metallic formation (Fe2O3, Al2O3, MnO2, MgO2 etc.) and oxide compositions (SiO2, CaO2 etc.). The aim of this project is investigate metallic content of steel slag used as fertilizer in plants. For this purpose, slag is used to determine the physical and chemical characterization. Then, it is separated from metallic content by using shaking table and multi gravity separative (MGS) method. After these procedures, there are determinate 6 groups with different densities and different grit size. Element analysis was made of these groups by atomic absorption spectrophotometer (AAS) and ICP-OES system. In addition, mineral analysis was determined by XRD device. Thus, within each group minerals have been identified. Slag-soil mixture was prepared for determination the highest yield of using six different slag groups as fertilizer in the plant. Thus, corn crops were grown in different combinations. Then harvested corn plants and done soil analysis of the plants. As a result of experiments, percentage of the most effective slag-soil composition for corn was determined. The highest yield in the slag pot was determined the rate of %12.5 and coarse-grained. Ideally, the slag content found to be chinerals such as merwinite (3CaO-MgO-2SiO2), akermanite (2CaO-MgO-2SiO2), gehlenite (2CaO-Al2O3-SiO2). Thus, it was detected the steel slags can be used as feed material for plants. Hence by maintaining the ecological balance economic contribution to the country was achieved.

星星相映-以理論與觀測探究雙星形成機制

由於在銀河系中的多數恆星是以雙星系統存在,因此雙星的形成機制在恆星演化理論中扮演極重要的角色。但目前其形成的機制未有定論,而爭論的焦點主要為兩個假說:一個假說為當分子雲在裂解為雲核時,同一雲核會形成兩顆恆星互繞;另一假說為不同雲核會分別塌縮為不同的恆星。這份研究中,我們以金斯最小質量與半徑為理論依據,找尋上萬筆的觀測數據,並對其資料來源做觀測限制上的確認,進而分析雙星間距的分布,且以雙星的星團與光譜型為分類作圖。分析後的結果中,我們發現前主序雙星間距分布圖出現了一個小於金斯最小半徑的峰值,得出了分裂說必定存在,然無法排除捕獲說的存在;並且發現不同星團、光譜型對間距的關係有顯著的差別。

利用光合作用機制研發光電材料之研究

葉綠素為植物體內進行光合作用關鍵物質,在能源領域內也有不少的應用。葉綠素是光合作用的鑰匙,葉綠素能夠累積光的能量並放出激發態電子,利用激發態電子的能量進行一連串複雜的反應,本研究的理論就建立在光合作用上,利用葉綠素吸收光能轉為電能的特性開發新型光電池。光電池使用金屬或非金屬材料,照光後能產生電流之材料作為發電材料。電池的製作過程非常簡單,實驗發電材料葉綠素也很容易取得,完全沒有汙染環境的疑慮,是非常乾淨的綠色能源。另外也探討葉綠素濃度對於發電效能的影響。研究使用的裝置是由兩片玻璃與矽膠片組裝而成的電池,在電池中間加入葉綠素與電解液。藉由改變電解液與有無光照來探討葉綠素在該電池中的氧化還原作用是否有明顯的變化,並推出化學反應式。結果顯示在葉綠素與電解液的配合下能夠建立出一套循環產電系統。