全國中小學科展

2013年

筆筒樹凋萎病防治法 -病原Ophiodiaporthe cyathease sp. nov.拮抗菌之篩選與應用

近在台灣新發現的筆筒樹萎凋病,造成大量筆筒樹(Cyathea lepifera)枯萎死亡,對於此疾病的防治已成亟需解決的問題。先前的研究中,已確認病原菌為新屬真菌並命名為Ophiodiaporthe cyatheae sp. nov.。本研究則在於以生物交互作用方式進行防治,尋找拮抗菌,針對特性實際應用。 研究中因實驗素材與操作方法的差異分為細菌與真菌類拮抗菌兩大主軸,且皆成功自土壤中各分離出數種有應用潛力的拮抗菌。細菌方面,尋獲並經鑑定為Burkholderia gladioli,對於病原菌有良好抑制效果,然由於此菌本身安全性有疑慮,實用上仍有待商榷。真菌方面,拮抗菌以超寄生方式與快速的生長能力制伏了病原菌,期待透過比較消除病原菌的速度,篩選出其中最強大適切的拮抗菌種,運用於實際田間病殘株處理。

The research of Ube anthocyanin characteristics and utilization

Anthocyanin is a water soluble pigment that may appear in various colors such as red, blue, and purple according to the pH. Ube is a fruit in the Philippines that is 3rd of the most cultivated crops. Since Ube contains a lot of anthocyanin, it helps to make the anthocyanin solution. As Anthocyanin reacts to the oxygen quickly it is also used as air pollution indicator because it changes its color according to what substances they are attached to it. To check if the solution reacts to the pollution such as SO2 and NO. When those pollutions are made artificially, examining the intensity of the anthocyanin solution depending on different Mol of the pollutions was able. The power of penetration of lights though the UV-VIS spectrophotometer increases according to the number of molecules of SO2, and also NO. To examine the change of intensity of anthocyanin solution in actual atmosphere, the solutions were exposed outside for several hours. The power of penetration decreases when exposed to an actual atmosphere. The other substances and oxidation were the causes of the changed in color of the solution To facilitate the usage of anthocyanin solution efficiently, it should be preserved, so that the density of specific color will be preserved when used as real air pollution indicator. In order to check what kinds of chemicals can preserve the anthocyanin solution; different kinds of strong acids, strong base, salts, and metals were added to the solution. After getting the data, the characteristics of the chemical which preserved the anthocyanin were scrutinized, than compared to the density of pure anthocyanin solution. To use anthocyanin as air pollution indication as a solid, the Korean traditional paper and cloth were dyed using ube which contains lots of anthocyanin than checked the pixel of red, green, and blue color. Firstly, chose 6 different salts. And then filter and boiled the anthocyanin solution. And then put each different salt in each paper. After that, dried the paper and check the difference of pixel of each paper. As a result, Calcium hydroxide (CaOH2) has highest pixel point. So, using calcium hydroxide to dye clothes is useful and it’s also useful for the air pollution indicator through the experiment. Especially it reacts to Nox and Sox, according to this experiment, it can use for eco-friendly air pollution indicator.

蟑螂心世界-利用心電圖與影像分析法探討昆蟲心臟因應體位變化的調節作用

本研究以心電圖(Electrocardiogram, ECG)與心臟影像分析(Image analysis)法,探討美洲蟑螂與短角外斑腿蝗的體位改變對其心臟生理活動的影響。當美洲蟑螂頭朝上立起時,血淋巴受重力影響而往身體尾端移動,此時心跳率增加,進而維持心輸出量。頭朝下立起時,血淋巴受重力影響而更易流向頭部,此時心搏量減少,進而減少心輸出量,以維持血淋巴循環的恆定。我們亦發現相對於心電圖記錄,影像分析法可收集較多參數,亦不傷害蟲體。總結前人文獻與本研究發現:人體(閉鎖式循環系統)主要透過動脈收縮以調節血壓,美洲蟑螂(開放式循環系統)主要改變心搏活動以維持血淋巴循環的恆定。

澱粉吸附蛋白質與澱粉糖的分子交互作用

顆粒澱粉可和碘形成藍色錯合物(starch-iodine complex),我從種子發芽澱粉酶實驗了解澱粉酶(amylase)具糖解酵素活性,會分解澱粉盤上的澱粉,並使澱粉失去與碘形成螺旋狀澱粉-碘錯合物的能力,讓藍色澱粉盤出現透明水解圈。通常澱粉酶之分子結構包含澱粉吸附區及催化區,分別負責吸附大分子或不可溶性澱粉糖(amylose)配體及分解澱粉糖成可溶性小分子糖。本計劃選用分子量、溶解度不同的澱粉糖分別與碘液反應,再加入澱粉酶(glucoamylase)或其澱粉吸附區(starch binding domain, SBD),發現二者均會破壞澱粉糖與碘結合之螺旋狀結構。分析二者之反應效率,可比較不同蛋白質解開澱粉-碘錯合物之能力。此外,本研究亦比較正常與突變株二聚體澱粉吸附區(SBD dimer)之解開澱粉-碘錯合物反應活性,再查詢文獻、比對不同蛋白質的序列、結構、及功能特性進行綜合分析。根據本研究結果,澱粉吸附蛋白質序列中解開配體螺旋結構最重要的官能基為兩個具有苯環的胺基酸,且二者對於可溶性及不可溶性配體之影響不同。本研究提供科學家了解自然界具備重複澱粉吸附區(tandem repeat)功能的邏輯方法,未來可應用於澱粉代謝、合成相關之蛋白質設計、結構預測、及功能分析。.

星星相映-以理論與觀測探究雙星形成機制

由於在銀河系中的多數恆星是以雙星系統存在,因此雙星的形成機制在恆星演化理論中扮演極重要的角色。但目前其形成的機制未有定論,而爭論的焦點主要為兩個假說:一個假說為當分子雲在裂解為雲核時,同一雲核會形成兩顆恆星互繞;另一假說為不同雲核會分別塌縮為不同的恆星。這份研究中,我們以金斯最小質量與半徑為理論依據,找尋上萬筆的觀測數據,並對其資料來源做觀測限制上的確認,進而分析雙星間距的分布,且以雙星的星團與光譜型為分類作圖。分析後的結果中,我們發現前主序雙星間距分布圖出現了一個小於金斯最小半徑的峰值,得出了分裂說必定存在,然無法排除捕獲說的存在;並且發現不同星團、光譜型對間距的關係有顯著的差別。

利用風洞分析微粒運動量-以蕨類孢子為例

本研究設計了兩個風洞實驗裝置,分為水平風洞與垂直風洞,兩者皆進行飄浮模擬試驗進而推算微小物質的運動量,並以小保麗龍球作為標準圓球,確保儀器的可用性,最後再透過醫檢儀器進行驗證。水平風洞利用機率的觀念統計孢子的分布,透過孢粉落下的高度差,帶入公式求得質量。垂直風洞則使用高倍率攝影鏡頭觀察孢子飛行,利用三力平衡的觀念推算其微小質量。最後,無論是自製風洞測出的質量、精確度、成本、測量速度和加速度的能力以及花費時間的長久,本實驗的儀器皆有優勢。 

Equipping, programming and testing a robot searching for an avalanche transceiver

1. Purpose of the research Because we live in a famous winter sport region in Switzerland, we have been confronted with the problematic of avalanches since we were born. In winter 2011/2012 alone, 179 people were involved in an avalanche accident, of whom 25 died. The most important device for searching and rescuing a buried person is the avalanche transceiver. It creates an electromagnetic field, which can be used to locate a buried person with another transceiver. The most important factor while searching is time: After 30 min. the chance of survival of a buried person has dropped to 40%. Considering the fact that people often make mistakes when they are put under such a big stress, valuable time gets lost. This is where our project comes into play. With an automation of the searching process a fast and reliable search should be achieved. The aim we agreed on for this paper is to develop a prototype of a robot that can find an avalanche transceiver automatically and reliably. To study and optimize the functionality of our robot more easily, our tests have only been performed on flat ground. 2. Procedures The components of an avalanche transceiver with only one transmitting antenna are used as a receiver. With the help of this receiver, the signal of a transmitting avalanche transceiver was analysed. Furthermore, the results were used to evaluate the most suitable search technique. Our robot is based on the “RP6” robot system, which is programmable in the C-language. The signal of the receiver is modified in a way which allows the robot to read it. Based on the results of the signal analysis, a search algorithm is developed. In the final tests the robot was examined as to its functionality and efficiency. Therefore, the robot was positioned at randomly chosen starting points to locate the transmitting device. 3. Data The transmitter could be located correctly in every test. The starting points were successfully located up to 11 m from the transmitter. The search ending points were found at a maximum range of 0.5 m from the correct position. 4. Conclusions In view of the results, the aim of developing an automatic localisation of an avalanche transceiver on flat ground as a prototype is seen as fulfilled. Outside the specified range, the transmitter can only be located unreliably. This is due to the fact that outside that range the signal differential in different directions is too small to be processed by the robot. Our prototype was tested under ideal conditions. Certainly, our robot is still some way from being ready for use in an actual avalanche. Many different questions have not been answered yet or have come up during this project, for example: ‘How should the robot should be applied on uneven terrain?’ or: ‘How should it deal with several buried people at once (multiple transmitted signals)?’ Two aspects of our prototype, the low weight and the low construction costs, however, are particularly favourable.

Building Bridges with Water-The Floating Waterbridge

This paper describes the investigation of a fascinating physical phenomenon called the “floating water bridge”. Despite the fact that water is undoubtedly the most important chemical substance on earth, it is practically ubiquitous and it still represents one of the best explored substances, still not all characteristics are well-understood. There are some phenomena like the “floating water bridge”, which cannot be explained. If high voltage is applied to two beakers, which are arranged close to each other and which are filled with deionized water, a connection forms spontaneously, giving the impression of a floating water bridge. For the experiment discussed in this paper, two beakers with a diameter of 50 mm and a height of 80 mm are filled with triply deionized water. Platinum electrodes are submerged in the center of the beakers, one set to ground potential (anode), the other one on high voltage, up to 25 kV dc. Within the scope of this work, an experimental setup was developed, which enables measuring and demonstrating the most important parameters like voltage, current, length and temperature of the water bridge as well as the mass transfer between the beakers. In addition the correlation between the different parameters and the influence on the water bridge could be estimated. Once the beakers are separated, the bridge remains stable for several hours up to a length of 2.5 cm. With platinum electrodes and no electrolysis observed, a small current (≈300µA), a mass flow from anode to cathode and forces were measured. Pictures, taken with an infrared camera and a new developed method to record "infrared-videos", enabled to visualize the heat flow in the water bridge. Furthermore the conversion of energy and the dependence of charge and mass transfer could be estimated roughly. In the course of the investigations it was also tried to prove the water bridge with other liquids like castor oil, olive oil, a mixture of glycol and water as well as tap water - for some of them for the first time. Supplementary the experimental setup was varied by using different electrodes with different sizes and different material as well as beakers of different sizes and materials. In addition, a qualitative explanation was developed. The results of this work enable a better understanding of the floating water bridge and provide a basis for further research as well as for development of future practical applications. One of these applications could be an improved waste water treatment process.

AI人工智慧-應用社群網站互動於類神經網絡訓練之研究

傳統的類神經網絡人工智慧多半是以受控訓練為主。然而在本次研究中,我們先建構出一套以類神經網絡模型為基礎的人工智慧,再利用社群網站噗浪(Plurk)上使用者與此系統的互動,訓練類神經網絡,以期驗證社群網站作為訓練來源的效率與準確度。我們利用分析詞、句的方式,促使系統做出自動的回應,同時並收集相關資料作為統計與修正之用。經過漸進式的調整與精進後,我們成功利用高度模組化的人工智慧系統,達成「利用社群網站資料自我修正」的目標,且其準確度呈現遞增的趨勢。我們相信只要充分掌握社群文化,社群網站做為資料來源對學術研究必有所裨益,且能為自然語言領域帶來更多可能性。

利用光合作用機制研發光電材料之研究

葉綠素為植物體內進行光合作用關鍵物質,在能源領域內也有不少的應用。葉綠素是光合作用的鑰匙,葉綠素能夠累積光的能量並放出激發態電子,利用激發態電子的能量進行一連串複雜的反應,本研究的理論就建立在光合作用上,利用葉綠素吸收光能轉為電能的特性開發新型光電池。光電池使用金屬或非金屬材料,照光後能產生電流之材料作為發電材料。電池的製作過程非常簡單,實驗發電材料葉綠素也很容易取得,完全沒有汙染環境的疑慮,是非常乾淨的綠色能源。另外也探討葉綠素濃度對於發電效能的影響。研究使用的裝置是由兩片玻璃與矽膠片組裝而成的電池,在電池中間加入葉綠素與電解液。藉由改變電解液與有無光照來探討葉綠素在該電池中的氧化還原作用是否有明顯的變化,並推出化學反應式。結果顯示在葉綠素與電解液的配合下能夠建立出一套循環產電系統。