熱線式渦流流量計
流量計在實驗室與工業領域裡是重要的儀器，如今已經有數十種依不同物理原理而發展出來的型式，可以配合多變的環境需求與測量條件而使用。然而，各種流量計所適用的範圍備受侷限。本研究主要目的在發展一種熱線式的渦流流量計，供給氣體之流量量測之用。透過自行製作儀器與設備：熱線測速儀(包括探針、探棒及電子處理器)和渦旋產生器(管道中含一三角形截面之鈍體，當流體通過時，在後方尾流產生週期性渦旋逸放)。由於熱線測速儀擁有偵測流體運動時高頻動態變化的能力(約為20000 Hz 以內)，因此結合熱線測速儀與渦旋產生器，經適當的設計與調校，可以測得在不同流體流速時渦旋產生器的三角截面鈍體後方渦旋逸放的頻率。由於渦旋產生器的截面面積為固定值，因此可以從而計算出流量與渦旋逸放頻率的關係。經由嚴格的校準與驗證步驟，本研究的結果顯示自製的熱線測速儀擁有極佳的渦旋頻率偵測能力，所量測到的校準曲線顯示渦旋產生器的三角形截面柱所產生的渦旋逸放頻率與流量成線性關係。為了降低誤差，建議在0 ~ 40 CMM 之量測範圍內分成三條方程式來代表不同範圍內的校準曲線，最大誤差僅在5%以下。若需使用在不同的流量範圍時，僅需改變渦流產生器和幾何尺寸，以使渦旋逸放頻率適合於熱線測速儀的動態響應範圍即可。倘若商品化之後，可以實際應用於風扇流量量測、引擎進氣埠流量的測量等等應用。熱線測速儀本身也可作為風速計，適用於各種場合之風速量測。Flow meter is a instrument that is vital to the laboratory as well as the industrial related field. Based on different physical principles, tens of models that work in harmony with the diverse environmental demands and measurement conditions are developed to date. However, the application of varied flow meters is still under severe restriction. The purpose of this study is to develop a hot-wire type of vortex shedding flow meter for the use of flow rate measurement. Through the home-made apparatus and device, the hot-wire anemometer (includes probe, stem and electronic processor) and the vortex generator. (duct that contains triangle’s section of the bluff body. When fluid passes through, the wake behind produces periodical vortex shedding.) The ability of hot-wire anemometer when it detects the fluid moving changes of high-frequent movement is within 2000Hz, after appropriate design and adjustment, the combination of hot-wire anemometer and vortex generator may investigate the frequency of different flow rate that generated from the vortex shedding behind the bluff body of triangle section. The section area of vortex generator is constant value, thus it can calculate the relationship of flow rate and the frequency of vortex shedding. By means of strict calibration and test procedure, the results reveal that home-made hot-wire anemometer has excellent ability to detect the frequency of vortex shedding. The calibration curve indicates a linear relationship between the frequency of vortex shedding and flow rate. In order to reduce inaccuracy, it is suggested to classify three formulas to represent the flow rate that ranges from 0 ~ 40 CMM. The greatest inaccuracy is under 5%. When applied to different flow rate range, it only has to change the size of vortex generator only if the response frequency of hot-wire anemometer suit for the range of frequency of vortex generator. After commercialization, it can be applied to measure the flow rate of fans, flow rate of intake valve of engine, etc. Hot-wire anemometer also served as anemometer, which can be applied to wind velocity measurement in any situation.
Using clay and paper fibre to treat waste water in the Bay Mau Lake in the Thong Nhat Park, Hanoi, V
The Bay Mau Lake inside the Thong Nhat Park in Hanoi is the second largest lake in\r Hanoi. The lake serves not just as an equitable tank for the rainwater, as a reservoir for\r intake of wastewater, as a wastewater treatment pond, but also as a recreation area for\r Hanoi people and visitors from other provinces. The Bay Mau Lake is polluted at\r alarming level.\r The author has carried out a study on the water pollution level and the sources of\r pollution. The study revealed that the lake polluted mainly by domestic wastewater\r discharging from living quarters with total about 800,000 inhabitants. The author\r demonstrated different measures to reduce the pollution level in the lake such as raising\r environmental awareness for the public, a simple, reasonable cheap measure, which can\r be both realistic and effective. Besides, the author proposed to use bio-treatment of the\r water discharging into the Bay Mau Lake, building of a pumping station, fish farming...\r The clay-paper fibre mixture is used for the biological treatment of wastewater at the\r inlets. The advantage of this method is that it is a simple, cheap and realistic solution.\r Treatment of the water using clay and paper fibre mixture for filtration and plantation of\r aquatic plants as lotus and water lily together will provide oxygen for the aquatic\r environment of the lake. As results of the study, it is recommended to use both methods,\r namely raising environmental awareness for the public and biotreatement method using\r clay and paper fibre, to improve water quality of the lake.
應用生物特徵及視覺密碼學防治車輛失竊和酒醉駕車
車輛遭竊以及酒醉駕車對社會安全造成的危害普遍存在於世界各先進國家。以目前看來，此問題並非嚴刑峻罰就能解決的。若本研究計畫將來普及應用於大眾，必定可以同時減少車輛遭竊之機會和預先防範酒醉者開車駕駛。 生物特徵泛指指紋、虹膜、臉型、聲紋甚至DNA 等等，它的特性即每個人均具有此獨一無二的性徵，加以應用就如同無法被仿造或偷竊的身分證明。若車主能利用自己的生物特徵為車輛上鎖，即可防止車輛遭竊。 視覺密碼學是屬於資訊隱藏技術的一種，藉由兩張以上的影像疊合後呈現其中隱藏之機密資料。雖然視覺密碼學原先純粹是為了加密資料，但我們將之加以修改，設計出複雜且無意義的偽裝影像，使其不易被酒醉後神智不清的人疊合成功而讀出其中的加密資料。 結合生物特徵及視覺密碼學，即可設計出一套可安裝於車上的系統，不僅防止車輛失竊又能防止酒醉駕車事故的發生。 ;The damage which is caused to social security by car stealing and drunk driving universally exist in others developed countries. As things are, these problems can not be solved by severe law. If our project can be applied in the masses extensively, it must be able to prevent both car stealing and drunk driving. Roughly speaking, biological characteristics mean fingerprint, iris, face, pronunciation and DNA. Their uniqueness is a special feature that everyone has. If we can apply it more, the characteristics are identification that can not be imitated or stolen. If the car owner can use his or her biological characteristics to lock the car, the car stealing could be prevented. Visual cryptography belongs to a kind of information hiding. By stacking two or more images, user can recognize the secret information hiding in the stacking. Although visual cryptography is merely created for encryption at first, we modify it and design a complex as well as meaningless stego-image. That makes the unconscious driver who is drunk fail to both stack the images and recognize the secret information hiding inside. We combine biological characteristics with visual cryptography and design a suit of system that is able to install in the car. This system can prevent not only car stealing but also drunk driving.
斑馬魚 SULT2 ST2在早期胚胎發育的 RNA 表現
在哺乳動物裡，硫酸化是一種參與外來物解毒作用、內生組織的荷爾蒙調節、藥物代謝及膽汁解毒之重要路徑。其中， SULTZ ( hydroxysteroid sulfotransferase ）是能進行上述反應的酵素之一，為了對 SULTZ 的功能與早期發育所扮演的角色作進一步的研究，在本實驗中，我們以班馬魚為模式動物，希望藉由原位雜交法（ in situ hybridization ）找到 SULTZ 在班馬魚早期胚胎發育的 RNA 表現位置。由目前的實驗結果，發現從卵巢到1-cell、 dome 、 50 ％一 epiboly 、 12 小時，都可在胚胎細胞看到訊號表現 · 在 24 小時、 36 小時、 48 小時、 72 小時則可在脊椎兩側體節的肌肉、內胚層、嗅窩、頭部看到訊號表現，此外，在成腦的原位雜交染色結果中亦看到了訊號表現。由此可推論 SULTZ 在斑馬魚早期發育確實扮演了重要的角色。Sulphonation is an important pathway for detoxification of xenobioties, bile acids, drug metabolism, and the regulation of endogenous hormones. SULT2 (hydroxysteroid sulfotransferase) is one of the enzymes which catalyse sulphonation. Zebrafish has emerged as a popular animal model in recent years. Compared with other vertebrates, it provides advantages including ease to get embryos, rapid external development, virtually transparent embryos and ease of genetic manipulation. The above-mentioned strong points made zebrafish a good model animal for us to understand the function of SULT2 during early embryonic development. We performed in situ hybridization to find out the RNA expression of SULT2 during zerbrafish early development. According to our present results, we can detect expression signal on the edge of telencephalon and tectum opticum. the edge on the dorsal zone of corpus cerebelli and ventral zone of periventircular hypothalamus of the adult brain. Besides, we can observe signal evenly distributed in blastocytes of the embryo at 1-cell, dome. 50%-epiboly and 12 hours after fertilization. We also find signal on the muscle next to the spinal cord during the stages of 24, 36, 48 and 72hrs. There are also expressions on hypoblast of embryos at 24, 36 and 72hrs, the olfactory pit at 36 and 4hrs, and strong expression in head region at 48 and 72hrs. These results suggest that SULT2 may have some function at the early development of zebrafish.
人面蜘蛛藉調整絲基因表現及網結構促進捕食效率
前人研究顯示結網性蜘蛛會因環境中獵物組成的改變而改變其網結構。然而，除了結構之外，構成網的絲的物理性質與蜘蛛網的捕捉效率也應有密切之關連，但目前並無人探討蜘蛛是否會因獵物而調整絲之化學及物理性質。在本研究中，我以人面蜘蛛為材料，探討：(一)其在面對不同類型之獵物時是否調整其網之結構，絲之胺基酸組成和其物理性質；(二)所產生之改變是否能促進不同類型獵物的捕食效率；以及(三)是獵物之養份或振動刺激引起這些改變。我藉著操控獵物的養份及振動刺激將人面蜘蛛分成四個處理組，分別餵以活蟋蟀(C 組)，活蒼蠅(F 組)，死蟋蟀加蒼蠅振動刺激(Cd 組)及死蒼蠅加蟋蟀振動刺激(Fd 組)。在完成食控處理後比較各組間之網結構、絲胺基酸組成、絲物理性質、對不同獵物之留置能力以及振動訊號傳遞能力。實驗結果顯示F 組之蜘蛛所結之網有較大的面積及較小的網目，並有較佳之蒼蠅留置力；然而此結構之改變卻會降低其振動訊號傳遞率。C 組之蜘蛛所結之網有較強之張力及較佳之蟋蟀留置力，而且其所產之曳絲有較高比例之alanine、glycine 及glutamine 並較佳之延伸度及韌度，以因應體型較大之獵物。上述結果顯示人面蜘蛛能調整網的結構、絲的蛋白質組成及物理性質來更有效率的捕捉不同類型的獵物。而由於上述各樣變數在C 及F 組間有顯著差異，而在獵物養份與振動訊號錯置的Cd 與Fd 組間無顯著差異，顯示此二項刺激需同時存在才能使人面蜘蛛在面對不同類型獵物時產生結網行為及絲基因表現之改變。Previous studies showed that orb-weaving spiders may adjust web structures when encountering different types of prey. In addition to web structures, silk physical properties should also be critical in affecting the prey catching efficiency of webs. However, so far no one has studied whether spiders adjust silk chemical and physical properties when encountering different types of prey. In this study, I used the giant wood spider Nephila pilipes to investigate (1) would N. pilipes adjust web structures, silk amino acid composition and silk physical properties when encountering different types of prey (2) would such changes enhance the catching efficiency of different prey and (3) were such changes generated by prey chemical composition or vibration signals? To answer these questions, I manipulated prey chemical composition and vibration signals by assigning N. pilipes into the following four feeding groups: (1) living crickets (group C), (2) living flies (group F), (3) dead cricket but with fly vibration signals (group Cd) and (4) dead flies but with cricket vibration signals (group Fd). After the feeding treatments were completed the following variables were compared between the feeding groups: web structures, silk amino acid composition, silk physical properties, retention time of different prey and vibration signal transmission rate of webs. The results showed that the webs built by spiders fed with living flies exhibited larger area, smaller mesh and longer fly retention time. However, such structural changes also reduced the vibration signal transmission ability of the web. On the other hand, webs built by spiders fed with living crickets were stiffer and retained crickets longer. Moreover, their silks exhibited higher percentages of alanine, glycine, glutamine and consequently higher elasticity and strength to cope with the larger prey. These results demonstrated that spiders can adjust web structures, silk protein composition and silk physical properties to enhance the catching efficiency of different prey. While the aforementioned variables differed significantly between spiders in C and F groups, they did not vary between spiders in Cd and Fd groups, in which groups the prey chemical composition and vibration signals were decoupled. Such result suggested that prey chemical composition and vibration signal were both needed in generating changes in web building behavior and silk gene expression in spiders.
探討以最經濟的方式偵測低濃度溶液之金屬離子
A novel technology for detection of trace concentration of ions at ppb level was developed. The detection limit of this method can be reached to 1ppb. The Wheatstone bridge usually be used for determination the concentration of metal ion, the major disadvantage of this technique is the precision too low. Atom absorption spectroscopy is better for monitoring trace metal ion, but the cost of instrument is too expensive. In this study, the oscilloscope was used to modify the Wheatstone bridge Circuit. The difference between the voltages of two connection points of Wheatstone bridge was used to estimate the concentration of ions. The amplification system was used in this method to improve the limit to detect ion from 10 ppm to 10ppb. The parameters of measurement including types of probe, match of Wheatstone bridge resistances, parameters of input signal and electrolysis in solution were also being studied. 本研究主要是發展一個富有創新且經濟的ppb ( parts per billion ; 10-9)級，微濃度金屬離子量策技術，目前本實驗設計已可以達到 ppb的偵測極限，尤其本方法不僅能夠應用於定量，尚可做定性偵測。簡易的溶液濃度技術常使用惠司通電橋的方法，但這個方法的缺點在於精確度較低，而另外常用的儀器是原子吸收光譜儀，卻價格高昂。因此，我們回頭對惠司通電橋的技術作改良和創新技術。首先，最重要的新技術是對示波器的改良。示波器被用來量測惠司通電橋的電位，我們比較電橋上兩特定點的電位來判斷濃度，當兩個點的電位相同時代表溶液電阻和控制電阻相同。但當輸入信號的電壓太強時，示波器上並無法判別細微的電位變化，因此造成量測的精度不高。本組設計新的電路用以放大在高強度訊號的波形細微變化，成功將測量極限由 10 ppm推進到 ppb。另外關於此設計中的各種參數，包括探針類型、惠司通電路的電阻匹配、輸入訊號之強度頻率、電極間電容效應及溶液中可能發生的電解效應等，都有精確的探討。最後的設計結果將是創新且有別於以往的設計概念。
台灣植物染與光敏有機太陽能電池
由於低廉成本、無毒及其他原因，由二氧化鈦、有機染料及電解液所製造的光敏有機太陽能電池(DSC)被預測即將成為一種大規模廣泛流行的太陽能電池。本實驗比較合成染料(像紅汞和疊氮離子)及成本較低且無毒的天然植物染料用於太陽能電池的效能。有些天然植物染料的效能大於合成染料的效能，像是紫色高麗菜、地瓜……等。在研製太陽能電池的過程中, 本研究利用自製的幾丁質燒結出的奈米顆粒成效極佳，不但粒徑大小符合，在燒結的過程中也不需經過物理研磨及介面活性劑的輔助，並且在低溫下即可燒結，並由BET 和SEM 測得幾丁質燒結的TiO2 的孔隙度大於標準燒結的TiO2 的孔隙度，因此本實驗決定利用去乙醯化之幾丁質扮演太陽能電池的二種角色:介面活性劑及保護劑。在本實驗中，部分的電池效能不盡理想，或許是因為所有的太陽電池樣本均使用相同的電解液(I3-)。要找到每種太陽能電池最適合的電解液則需要更多的研究。Because of the desirable features of low cost, non-toxin, and other reasons a dye-sensitized nanostructured solar cell (DSC) composed of nanocrystalline TiO2, organic dyes and electroiytes is expected to be in prevalent use. This study compared the differences in the functioning of different dyes in the solar cells, specifically those between the synthetic dyes, such as Merchrochronate and Polypyridyl complexes of ruthenium (N3-), and dyes made of natural plants which cost less and are not toxic. Some natural dyes, such as purple cabbage, sweet potato, etc, were found to have better efficiency than the synthetic dyes. The study makes use of self-made chitin to sinter nano particles, and the result is excellent. First, the particle radius meets the size requirements. Second, in the process of sintering nano particles, no physical pulverizing or surfactant assistance was needed. Third, the nano particles can be sintered at a low temperature. Fourth, from BET and SEM measurements, the degree of aperture of TiO2 sintered with chitin is superior to that sintered with Triton X-100. For these reasons, the de-acetylated chitin was chosen to serve as surfactant assistance and protector in the solar cells in this study. The study also found that some solar cells were not efficient enough. Maybe it is because all the samples of solar cells in this study were produced with the same electrolyte (I3-). Further study is needed to figure out the most suitable electrolytes for each solar cell.
Self Assembly Mechanism of Water Drotlets
這是一系列關於水蒸氣冷凝為極細微小水珠的長程實驗。其中可以分為下列三個階段：第一階段是基礎實驗。將水氣導入至潔淨的光滑表面上(蓋玻片)，觀察水珠冷凝的機制。第二階段是在外加磁場及電場作用下，將水氣導入至潔淨的光滑表面（蓋玻片），觀察水珠冷凝的機制。這部分的實驗推翻了一般「水分子是電中性，在電場或磁場中不受影響 」的刻板觀念！實驗所呈現出來的冷凝水珠，不但有明確的自我組成模式( Self assembly pattern)。並且發現：電場會增速凝結水珠的成長(Aggregation)，而磁場則會抑制凝結水珠的成長。第三階段是將水蒸氣導引至超聲波的環境中：我們先將超聲波訊號產生器(變頻、定頻)面向於載台旁，再讓水氣導入至潔淨的光滑表面上(蓋玻片)，觀察冷凝水珠的機制。當使用固定頻率超聲波波源，我們發現：在超聲波場中水珠的成長會受到抑制，且成長速率會隨著頻率的升高而逐漸減小。第一階段與第二階段的實驗結果與討論已分別發表於2004 年及2005 年的台灣國際科學展覽報告中，本作品將詳述第三階段。 This experiment explores the basic nature of the condensation of water vapor into droplets on the surfaces of cover glasses. This condensation occurs because of the difference in temperature between the water vapor and the cover glass. The condensation process is observed under a microscope. The growth of the droplets can be described as: nucleation, aggregation (piling up) and coalescence. The growth is irrelevant to surfaces or environments. It is found that the temperature difference of moist air over the cover glass do not affect the nucleation size of the droplets in simple plain surroundings; while the change of flow rate does. In general, the coalescence is speeded up at higher temperatures. Furthermore, the effects of electric fields 、magnetic fields and ultrasonic waves are also studied. It can be observed that the size of water droplets become smaller and grow more uniformly under magnetic fields or imposed ultrasonic waves; also, the aggregation rate is decreased by imposed magnetic fields or ultrasonic waves, and it is increased by imposed electric fields. These effects of magnetic fields 、electric fields and imposed ultrasonic waves might be related to the flow conditions and the vibration of surrounding air in the system. This experiment provides the first step in the understanding of the formation of water droplets and their self assembly mechanism in different environment.