2005年

由於結網性蜘蛛視覺不靈敏，如何在網上藉振動進行獵捕，這是長久以來頗令科學家困惑的難題，當周遭環境各種振源觸網時，首先會產生不同振盪，蜘蛛是否藉由這些振盪得知獵物資訊？如何迅速準確的定位？又有那些決策條件影響蜘蛛的捕獵行為？更特別的，為何蜘蛛在捕獵過程中會“扯網”？本研究以台灣最大型結網性蜘蛛－人面蜘蛛為研究對象，並設計出一套非接觸式的測量方法，就上述謎題作深入的探討後，成功的解開人面蜘蛛的捕獵機制。簡單來說，其機制分為兩大系統：(1)當獵物擾動不明顯，人面蜘蛛會立即扯網，藉有無產生阻尼振盪，以判斷有無獵物存在；在阻尼振盪產生時，蜘蛛將感知其中具有最大阻尼振盪之放射絲為獵物所在方向，而振盪週期長短，係蜘蛛用以判斷獵物遠近之有效因素。(2)當振源明顯時，蜘蛛直接判斷各種擾動的振幅大小、頻率高低、波形模式、振源質量輕重，決定是否啟動捕獵或逃離反應，並在反應前先行定位，亦即以步足腳勾偵測並比較各放射絲之振盪大小，以振盪最大之放射絲為獵物方向，其次藉由第二對步足之位移所產生之準光角，判斷獵物之遠近。蜘蛛正確的將獵物定位後，會以適當的速度往前衝，一口咬住獵物，以蛛絲重重包裹後，拖往網中央並進行吸食。 Giant wood spider, Nephila pilipes, is the biggest orb web spider in Taiwan. The mature N. pilipes may even grow to exceed 5 cm body length. While waiting for the prey, its giant body hangs quietly on the hub of the web. Owing to its ineffective vision and sense of smell, the spider depends almost on detecting the vibration signal of the struggling of web cause by the struggling prey. When various kinds of sources from the environment contact the web, they will generate various types of vibrations which cause the spider to judge whether they represent danger, prey, or irrelevant signals. Our results suggest that if the disturbance is obvious, through discriminating the amplitude and frequency of the vibration, the spider will make a decision whether to attack or escape immediately. Yet, before any decision is made, it will need to locate the source of vibration. For example, it will locate prey correctly by comparing the vibration transmitted from the radiating strings. The radiating strings that transmitted the largest vibration are where the prey is entangled. The displacement of the second pair of legs will generate a quasi visual angle which enables it to comprehend the distance of prey. When the vibration signal is obscure, it will jerk the radiating string immediately. After jerking it, if there is damping oscillation on the web, then the spider can judge the location of the prey. When there is damping oscillation, the radiating string that transmitted the greatest damping oscillation is where the prey is entangled. Furthermore, the frequency of damping oscillation helps the spider to judge the distance of the prey. After locating the prey correctly, N. pilipes approaches the prey fast, wraps it with silk then drags the prey to the hub to feed.\r

利用陰極電弧蒸鍍各種薄膜，如：類鑽膜（DLC）、氮化鈦膜（TiN）、氮化鉻膜（CrN）、氮化鋁鈦膜（TiAlN）以及先披覆上一層氮化鋁鈦膜（TiAlN）再加上類鑽膜（DLC）的合成膜等。這些薄膜現在已經被廣泛的應用於各種刀具、模具的表面處理之中。本研究主要在探討高速鋼鍍上氮化鉻膜（CrN）之後，對於硬度、磨耗性質的改變，以及觀察氮化鉻膜（CrN）表面結構之組織。 在研究中我們運用陰極電弧蒸鍍系統蒸鍍氮化鉻薄膜，分析上運用SEM來觀察薄膜表面結構組織，以及運用洛氏微硬度機來觀察試片的硬度，另外還有使用磨耗試驗機來進行磨耗測試。以上這些測試總括來說都是在得知性質有無實際上的改變，而這些實際上的改變對於蒸鍍之後的模具或刀具都能夠大幅的提高使用的壽命。 We evaporated different kinds of thin films by using the anode of the electronic arc, such as DLC (Diamond-Like Carbon), TiN (Titanium Nitride), CrN (Chromium Nitride), TiAlN (Titanium Aluminum Nitride), and synthetic films of covering TiAlN and DLC. These thin films have been used widely in processing the surface of a variety of cutters and moulds. The purposes of this research were to investigate changes of hardness and abrasion and to observe the organization of the surface structure of CrN after High-speed steel evaporates CrN. In this study, we use the system of the anode of electronic arc to evaporate CrN. Besides, SEM is used to observe the organization of the surface structure of the thin films and Rockwell Micro-hardness Test Machine is used to investigate hardness of testing samples. Moreover, we use Abrasion Tester to test abrasion. These tests are taken to lead to a better understanding whether the quality really changed. These changes of evaporated moulds or cutters would extend their frequency of using.

流行病學資料顯示，近年來口腔癌發生率明顯增加。本研究之目的是探討口腔黏膜細胞微核發生頻率及彗星分析法之基因傷害指標是否與菸、酒、檳榔暴露有關，以及是否可以看到菸、酒、檳榔之交互作用。 研究對象為桃園地區山地鄉之民眾，利用問卷收集個人之菸、酒、檳榔及干擾因子之暴露資料，再採集樣本之口腔黏膜細胞及血液樣本，進行基因傷害評估。 結果顯示，口腔黏膜之微核發生率與檳榔之暴露相關性最大，可以解釋12.6％；然而，彗星尾部動量和尾部長度則以香菸暴露解釋力最大；同時，檳榔與香菸的基因傷害交互作用似乎存在。因此我們認為，口腔黏膜細胞之微核發生頻率對於檳榔暴露者應是不錯的基因傷害指標，而香菸暴露則以彗星分析法之指標較為敏感。 ;Epidemiological studies showed that the incidence of oral cancer increased significantly in recent years. The purpose of this study is to evaluate the genotoxicity of cigarette smoke, alcohol and betel quid use as well as the. interaction effect between these three risk factors. The study subjects were selected from residents lived in a mountain area of Tao-Yuan county. We used questionnaire as a tool of exposure measurement. The exfoliated oral buccal cells and blood samples were collected for the assay of micronucleus frequency (MNF) and comet assay. We find that there are higher MNF in smokers, alcohol drinkers, and betel quid users compared with nonusers. However, a significant difference can not be found because of small sample size. The relationship between MNF and three risk factors was strongest in betel quid use, however, for smokers the parameters in comet assay were more sensitive than MNF. There is a tendency of additive effect between cigarette, alcohol and betel quid although without statistical significance. Advanced statistical analysis is suggested to find stronger evidence in the genotoxicity biomarker.

The study is to investigate Taiwanese tardigrades,and the research of that is few. Tardigrades are commonly called water bears and have been identified more than 750 species. Limno-terrestrial tardigrades are small, 0.2-0.5 mm in length, and mostly found in moss cushions growing on rocks, soil, or the wall of houses. When the environment dehydrates in dry weather, Tardigrades desiccate into a reversible state of metabolic suspension called cryptobiosis. We have been finding a number of tardigrades in moss at many places in Taoyuan country. First, we put the moss on the dissected microscope to seek for tardigrades, and then placed it on the microscope for photographing and observing. The study is mainly focus on Taiwanese tardigrades. we have classified 11 Taiwanese species in four families（Echiniscidae,Calohypsibiidae,Milnesiidae and Macrobiotidae）, making Chinese keys of classification. From the habitat envoriment, the species, the density and the diversity we survey as well as the most suitable pH envoriment we experiment, we approach the relationship between the distribution of tardigrades and their habitat. Besides, we also research lots of conditions which bring cryptobiosis and make culture medium in order to inspect its living. These results indicate that tardigrades desiccate into cryptobiosis in ten munites in acid rain(pH4.65). From outdoor surveys, we have noticed tardigrades can’t be found in the moss right next to road.The length of each family is: Calohypsibiidae＞Milnesiidae Milnesium＞Macrobiotidae＞Echiniscidae. 此研究是探討台灣熊蟲，而有關台灣熊蟲的文獻資料極少。熊蟲屬於緩步門，體長約 0.2-0.5 mm，熊蟲在不利的環境會蟄伏，環境有利時又會膨脹而復甦，而其構造系統不因此而破壞。 我們在桃園縣多處的苔蘚發現熊蟲，我們先將採集的苔蘚放至解剖顯微鏡下尋找熊蟲，再由複式顯微鏡觀察構造並拍照紀錄。研究主要是探討台灣本地的熊蟲，我們已分類出十一種台灣熊蟲，製作中文檢索表。藉由觀測採集環境和所測的密度、歧異度和種類，及實驗出其最適宜的 pH值，探討環境對其分布的影響。也探討各種因素與蟄伏的關係，製作培養基以觀測其生活史。研究結果顯示：1.已經分類出台灣有緩步門四科（端爪科、Calohypsibiidae科、Milnesiidae科和 Macrobiotidae科）十一種熊蟲。2. 污染嚴重或環境髒亂的地方，不會有熊蟲的存在，且熊蟲有群居性。3. 端爪科(棕色)熊蟲在 pH4.65（台灣都會區雨的酸鹼值）以下的液體環境活動力明顯降低。4.熊蟲多分布在高溼度（87.5~90.4％）的地方，不分布在中低溼度 （76％以下） 的地區。5. 各科的體長為 Calohypsibiidae科＞Milnesiidae科 Milnesium屬＞Macrobiotidae科＞端爪科。

我們的研究包括兩部份，第一部份是實地調查訪問。瞭解地震成因、傷害及現有防震方法，並調查坊間各種建築物類型，及常見私自改變建築物結構現象，做為研究的基礎。第二部份為建築物抗震實驗。研究發現：牆面挖空、頂樓加蓋、樓層挑高，建築物會在該處產生弱點，由此斷裂。柱子數量相同下，散開時支撐力較弱。不對稱建築物遇震時會不自然扭轉且易倒。隔震素材恰當，能有效提高耐震力。滑軌、彈珠隔震效果很好，但位移太大，為實際建築所不容許。建物下加裝阻尼材料，能吸收部份地震能量，降低地震對建築物的危害，並有效控制位移問題，是良好的隔震素材。樓頂加裝消能設施亦能減震，但設計極其不易。 The research includes two parts. In the first part the work is concentrated on on-site visiting and investigation such as understanding the cause of earthquake, the damage and the preventive method currently available, investigating the different types of building and the phenomenon of altering the structure of an existing building without permission by government authorities which is popularly seen in Taiwan. These are considered as the basis of the research. The second part is the experimental study of earthquake resistance of a building. The test results showed that weak-point can be caused at the place where the existing wall is moved or an extra building is attached to the roof or the structure of building has extended space between floors, and fracture always occurs at the weak-point. If the number of columns of a building is the same, then the scattering arranged location of columns is weaker than concentrating type of arrangement of columns. The building having unsymmetrical structure will twist in uneven fashion that causes the building apt to collapse in case of earthquake. Employing proper vibration-absorption material can effectively increase earthquake resistance. Sliding rails and balls can provide satisfiable vibration-isolation effect, but can also cause too much displacement of building structure. Install damping material beneath the building can absorb part of the energy of earthquake, and decrease the damage, and can solve the problem of displacement of building, therefore, damping material can be considered as an ideal vibration-isolation material. Install energy-attenuation equipment can also reduce vibration but the design of the equipment is extremely difficult.

Up to this time we have spent almost three years in studying condensation and water droplets. Little could we have done as compared with the almighty nature. However we are rewarded by the nature as we gradually found the secrets about electro-magneto field and water droplets: The size of water droplets turn smaller upon electro-magneto field and grow more uniformly especially upon electric field. This experiment presented here is actually the diary of the growth of water droplets in condensation, upon magnetic field and electric field. Through convection, it discusses the self assembly patterns of water droplets and peep into the uniformity both of the size and the distribution mode of water droplets. In former basic experiment, we focus on temperature and the speed of water moisture; generally speaking, higher temperature speeds up the coalescence procedure but does not affects the nucleation size of water droplets in simple plain surroundings; while speed of moisture does affects the nucleation size. As we went farther, deep into convection and found magneto-electric force did play an important role in the self assembly mechanism of water droplets. The topic is mostly concerned as we are surrounded by magneto-electric waves in today’s world. This experiment anchors the first step in discovering the uniformity of water droplets in different environment, and providing insights into the self assembly mechanism of water droplets upon electro-magneto field with nano sizes. 這是一系列關於水蒸氣冷凝為極細微小水珠的實驗。其中可以分為兩大部分； 第一部分是基礎實驗。將水蒸氣導入至潔淨的介面上(蓋玻片)，觀察冷凝水珠的結構。雖然看似簡單平常，但卻有令人驚奇的發現；不同溫度的水蒸氣，其冷凝最初始的細微顆粒之尺寸是相同的 ！爾後隨著溫度的升高，堆疊速率也跟著上升；以致於最後一起呈現出來的水珠大小不一，尺寸不一。 第二部分是將水蒸氣導到磁場及靜電場上，觀察其冷凝結構。這部分的實驗推翻了一般「水分子是電中性在電磁場中不受影響？」的刻板觀念 ！實驗所呈現出來的冷凝水珠，不但於附加磁場中尺寸縮小又不易長大，同時還有固定的自我組成模式( Slef-assembly pattern);而且也發現在磁場中的冷凝小水珠的尺寸比電場中的小，可是電場中的小水珠則表現出較大的均勻特質。

During the evolution of humankind, development of frontal cortex has played a critical role, where higher brain function like emotions, self-consciousness, decisions…etc, were all related to frontal cortex. On the other hand, thalamus is usually associated with relaying the sensory signals from peripheral receptors. In order to understand the functional role of frontal cortex, the signal processing mechanisms in the thalamo-frontal cortical pathway became an important research issue. The aim of this experiment was to find a method to dissect a brain slice that contains a connecting route in vitro between thalamus and frontal cortex with functional activity. Through nerve fibers tracking technique using fluorescent-dye (DiI), it was understood that the 3D-space connection between thalamus and frontal surface was an upward curve with a turn of about 110 degrees and bending inwards from the two sides. If a conventional horizontal section was performed, the route would be cut-off and its integrity lost regardless of the direction. To solve this problem, a novel section method was developed to retain the route. Based on the route direction shown by the fluorescent-dye, a piece of brain block was cut and flattened of about 110 degrees. Other sections were performed as control for studying the effectiveness of the sectioning on the plane of the route. Finally, electrophysiological methods were used to verify the connection route was complete and functional. Thalamus-evoked extracellular field potentials in the frontal cortex were observed by changing stimulation strength, adjusting slice temperature and prepared oxygen supply and administration of drugs like CNQX and picrotoxin in the 110 degree flattened slice but not the others. It was found that the reaction was essentially a neuronal response, indicating the pathway between thalamus and frontal surface was retained substantially. With this novel brain slice technique, we can assess the functional connection between thalamus and frontal cortex and investigate the cellular mechanisms of the signal processing in this connection pathway. It is anticipated that present technique provides an important step to further elucidate the functional role of the frontal cortex. 在人類的演化史上，前額葉的發展扮演了極為重要的角色，凡舉情緒、自我意識、決策等，皆與前額葉有關。而丘腦通常與視覺、聽覺及本體感受如痛覺、觸覺、溫度覺的訊息傳遞有關。要了解前額葉的功能，丘腦到前額葉的徑路及訊息處理機制，便成為一個很重要的研究課題。本實驗的目的是尋找一個方法能在離體的腦切片上維持具有丘腦到前額葉連結的徑路並且有功能的活性。經由螢光染料(DiI)神經束追蹤技術，了解從丘腦至前額葉路徑的三度空間連結為一先向下再向上約110度的角度轉折，並同時先由內向外再轉向內的曲線，若用一般水平之切片方法，無論何種方向，其路徑必定會被切斷，不能保持其完整性。為了解決此問題，發展出一種可以保存其路徑的腦切片方法。依照螢光染料所顯示出的的路徑走向，在腦塊的皮質上切一刻痕，將腦塊以110度的角度展平，使其路徑處在一個平面上再切片。最後利用電生理的方法來證明所切出的連結路徑是完整且具有活性。改變在丘腦的刺激強度、調整腦切片(腦脊髓液)溫度、氧氣的供給以及施予藥物CNQX、picrotoxin，觀察其前額葉之電位變化，發現其反應確實為神經反應，表示從丘腦至前額葉的路徑已在這種特殊的腦切片中被完整保存。藉由這個方法，將有助於研究丘腦至前額葉功能性連結，神經網路結構，及其訊息處理機制，並期待以這樣一個全新的方法將來有助於瞭解前額葉的功能。

Chitin, a polysaccharide common in biocomposites, has an interwoven organic framework that can act as a scaffold for mineralization in natural systems. Acidic amino acids, namely aspartic (Asp) and glutamic (Glu) acids, are the primary active molecules at biomineralisation interphases in mollusks and play an important role in controlling the polymorph and morphology of the associated mineral.\r In this study, chitin was extracted from squid pens and used as a scaffold for crystallization. The chitin scaffold was functionalized with glutamic acid and aspartic acid separately to create an artificial microenvironment for the study of biomineralization of calcium carbonate. The objective was to investigate the usefulness of the extracted chitin to serve as a scaffold for calcium carbonate crystallization, especially the fidelity of the polymorph nucleated to the amino acids and proteins used.

Based on Ampere，s Law, the magnetic field intensity of the solenoids is B=μ0μr?n?I, where μ0 is the magnetic permeability of free space, μr is the relative magnetic permeability, n is the number of coils per unit length and I is the solenoidal current. The end magnetic field of the solenoid must multiply by one half. According to the above result, it can be greatly strengthened by the addition of a ferromagnetic core. First, we observe three different inserted materials of coils (wood, iron and magnetite), whose magnetic induction in different solenoidial current. By experiment, when the iron and magnetite materials were inserted into the coil, it would produce larger magnetic induction. The calculated relative magnetic permeabilities of wood, iron and magnetite materials are 0.57, 18.37 and 18.32, which are close to the reported paper (1). When the driving field is removed, the fraction of the saturation magnetization of the magnetite is retained, which is called hysteresis and is related to the existence of magnetic domains in the material. In the second part, we change the frequency of circuit switch, which induced different current. Compared with the result of the first part, it would fit the result, which is the induced magnetic field is proportion to the solenoidal current. 根據安培定律，螺線管的磁場為B=μ0μr?n?I。其中μ0為真空中的導磁率，μr為相對的導磁率，n為單位長度的線圈匝數，I則為通入螺旋管的電流。至於螺旋管的端點磁場須再乘上1/2。所以根據上述的結果，當螺旋管插入鐵磁性物質，會增強螺旋管的磁場。首先，觀察三種不同的芯物質；非鐵磁性材料，軟磁材料，硬磁材料（木棒，低碳鋼棒，磁鐵棒）在不同的外加磁場下的感應磁場，得到芯物質的磁化曲線，而計算出來的相對導磁率分別為0.57, 18.37 和18.32與參考文獻(1)接近。而當外加磁場移走時，硬磁性物質的磁性仍然存在，稱為殘磁現象。在第二部分，我們改變線路開關的頻率。發現不同的開關頻率，會得到不同的螺旋管電流，而造成不同的感應磁場。再度驗證了感應磁場大小是正比於螺旋管電流的大小。

本文中我們探討一個有趣的數列。這個數列有一個非常特殊的性質：將數列相鄰兩項的前項當分子，後項當分母，所產生的分數數列，恰好會出現所有的正有理數。 這個特殊的性質表示，可以將正有理數按照這個方式作排序，這個排序將完全不同於常見的正有理數排序的方法。 (1). 在正有理數的排序的結構中，我們做出許多有關於此數列的定理。 (2). 用數學歸納法證明此分數數列涵蓋所有正有理數，且每一正有理數只出現過一次。 (3). 將數列分割後，利用試算表製成數列規則表，並整理出快速的方法將數列表達出來。 (4). 將an 數列排成“樹＂的模式，可更快速的把正有理數寫下來。 (5). 最後，設計出搜尋正有理數的演算法，解決在分數數列中第n個正有理數會是多少；以及正有理數會出現在數列中第幾項的問題。 Let’s discuss an interesting sequence. There is a very special quality in it. In this sequence, choose two numbers, which are close to each other, and suppose the first number as “member” while the second one as “denominator.” Then we can get a fraction sequence that includes all of the positive rational numbers! According to this special quality, we can arrange positive rational numbers by the following method. Then we can get a brand-new way of the arrangements. (1). We can find many theorems about this sequence according to this special arrangement of the positive rational numbers. (2). We can prove the rule that this fraction sequence includes all of the positive rational numbers by mathematical induction. Furthermore, every positive rational number appears only once. (3). After dividing this sequence into several parts, we can get a sequence rule list by using trial balance and find a faster method to express the sequence. (4). Arrange the an sequence by the tree model. By this way, we can get all of the positive rational numbers much faster. (5). Finally, we can develop the operation method to solve the questions that what position would one positive rational number be in the sequence and what is the first, second, third or nth positive rational number of the sequence.