緩步門的木乃伊-會蟄伏的熊蟲
The study is to investigate Taiwanese tardigrades,and the research of that is few. Tardigrades are commonly called water bears and have been identified more than 750 species. Limno-terrestrial tardigrades are small, 0.2-0.5 mm in length, and mostly found in moss cushions growing on rocks, soil, or the wall of houses. When the environment dehydrates in dry weather, Tardigrades desiccate into a reversible state of metabolic suspension called cryptobiosis. We have been finding a number of tardigrades in moss at many places in Taoyuan country. First, we put the moss on the dissected microscope to seek for tardigrades, and then placed it on the microscope for photographing and observing. The study is mainly focus on Taiwanese tardigrades. we have classified 11 Taiwanese species in four families（Echiniscidae,Calohypsibiidae,Milnesiidae and Macrobiotidae）, making Chinese keys of classification. From the habitat envoriment, the species, the density and the diversity we survey as well as the most suitable pH envoriment we experiment, we approach the relationship between the distribution of tardigrades and their habitat. Besides, we also research lots of conditions which bring cryptobiosis and make culture medium in order to inspect its living. These results indicate that tardigrades desiccate into cryptobiosis in ten munites in acid rain(pH4.65). From outdoor surveys, we have noticed tardigrades can’t be found in the moss right next to road.The length of each family is: Calohypsibiidae＞Milnesiidae Milnesium＞Macrobiotidae＞Echiniscidae. 此研究是探討台灣熊蟲，而有關台灣熊蟲的文獻資料極少。熊蟲屬於緩步門，體長約 0.2-0.5 mm，熊蟲在不利的環境會蟄伏，環境有利時又會膨脹而復甦，而其構造系統不因此而破壞。 我們在桃園縣多處的苔蘚發現熊蟲，我們先將採集的苔蘚放至解剖顯微鏡下尋找熊蟲，再由複式顯微鏡觀察構造並拍照紀錄。研究主要是探討台灣本地的熊蟲，我們已分類出十一種台灣熊蟲，製作中文檢索表。藉由觀測採集環境和所測的密度、歧異度和種類，及實驗出其最適宜的 pH值，探討環境對其分布的影響。也探討各種因素與蟄伏的關係，製作培養基以觀測其生活史。研究結果顯示：1.已經分類出台灣有緩步門四科（端爪科、Calohypsibiidae科、Milnesiidae科和 Macrobiotidae科）十一種熊蟲。2. 污染嚴重或環境髒亂的地方，不會有熊蟲的存在，且熊蟲有群居性。3. 端爪科(棕色)熊蟲在 pH4.65（台灣都會區雨的酸鹼值）以下的液體環境活動力明顯降低。4.熊蟲多分布在高溼度（87.5~90.4％）的地方，不分布在中低溼度 （76％以下） 的地區。5. 各科的體長為 Calohypsibiidae科＞Milnesiidae科 Milnesium屬＞Macrobiotidae科＞端爪科。
吸菸、喝酒與嚼檳榔之基因毒性研究
流行病學資料顯示，近年來口腔癌發生率明顯增加。本研究之目的是探討口腔黏膜細胞微核發生頻率及彗星分析法之基因傷害指標是否與菸、酒、檳榔暴露有關，以及是否可以看到菸、酒、檳榔之交互作用。 研究對象為桃園地區山地鄉之民眾，利用問卷收集個人之菸、酒、檳榔及干擾因子之暴露資料，再採集樣本之口腔黏膜細胞及血液樣本，進行基因傷害評估。 結果顯示，口腔黏膜之微核發生率與檳榔之暴露相關性最大，可以解釋12.6％；然而，彗星尾部動量和尾部長度則以香菸暴露解釋力最大；同時，檳榔與香菸的基因傷害交互作用似乎存在。因此我們認為，口腔黏膜細胞之微核發生頻率對於檳榔暴露者應是不錯的基因傷害指標，而香菸暴露則以彗星分析法之指標較為敏感。 ;Epidemiological studies showed that the incidence of oral cancer increased significantly in recent years. The purpose of this study is to evaluate the genotoxicity of cigarette smoke, alcohol and betel quid use as well as the. interaction effect between these three risk factors. The study subjects were selected from residents lived in a mountain area of Tao-Yuan county. We used questionnaire as a tool of exposure measurement. The exfoliated oral buccal cells and blood samples were collected for the assay of micronucleus frequency (MNF) and comet assay. We find that there are higher MNF in smokers, alcohol drinkers, and betel quid users compared with nonusers. However, a significant difference can not be found because of small sample size. The relationship between MNF and three risk factors was strongest in betel quid use, however, for smokers the parameters in comet assay were more sensitive than MNF. There is a tendency of additive effect between cigarette, alcohol and betel quid although without statistical significance. Advanced statistical analysis is suggested to find stronger evidence in the genotoxicity biomarker.
終端速度
液體中之球體運動與液體的黏滯性有關，本實驗找出球體半徑與終端速度之間的關係。利用攝錄機作為紀錄工具，拍攝三種材質（壓克力、玻璃、水晶）的球體在沙拉油中的自由落體過程。使用電腦影像處理軟體將影像分解成幅影像，時間的解析度為1/30秒。測量球體的高度與時間，分析高度與時間的變化情形，發現終端速度與球體半徑之間的關係。
流體中之運動方程Fdrag = -k1V，無法符合實驗結果。我們的實驗結果顯示油中的自由落體的運動方程應該是Fdrag = -（k1V+ k2V2）。由不同材質的壓克力球（~1.18g/cm3）、玻璃珠（~2.47g/cm3）與水晶球（~2.66g/cm3）所獲得的終端速度（Vt）與球體半徑（a）的關係為a3(ρ-ρ') = 0.00003（a Vt）2 + 0.00021（a Vt） + 0.00575，其中ρ與ρ'分別為球體密度與沙拉油密度（0.90 g/cm3）。
再者，在相同半徑的條件下，密度越大的球體終端速度越大，在靜止下落後，越久達到終端速度。
The motion of a sphere which is falling through a fluid is subject to the fluid viscosity. In this study, we find out the relation between the radius of a sphere and the terminal velocity. We used a digital camera to record the sphere's descent in the oil. The three kinds of sphere we choose are acrylic（~1.18g/cm3）, glass （~2.47g/cm3）and crystal （~2.66g/cm3）. Frame-by-frame analysis of the video footage yielded rough estimates of the sphere's location within 1/30 seconds accuracy for statistically consistent results. By measuring the location and time and analyzing them, we find out the relation between the radius of a sphere and the terminal velocity. The expression of the drag force,Fdrag = -k1V, is not cosistent with our results. The study indicates the expression of the drag force should be Fdrag = -（k1V+ k2V2）. The expression for the terminal velocities of the three kinds of sphere is of the form：a3(ρ-ρ') = 0.00003（a Vt）2 + 0.00021（a Vt）+ 0.00575, where a is the radius, ρ is the sphere density and ρ' is the oil density（0.90 g/cm3）. In addition, if the radius is the same, the terminal velocity of a denser sphere is higher and the time to approches the terminal velocity is longer.
水分子自我組裝之機制探討
Up to this time we have spent almost three years in studying condensation and water droplets. Little could we have done as compared with the almighty nature. However we are rewarded by the nature as we gradually found the secrets about electro-magneto field and water droplets: The size of water droplets turn smaller upon electro-magneto field and grow more uniformly especially upon electric field. This experiment presented here is actually the diary of the growth of water droplets in condensation, upon magnetic field and electric field. Through convection, it discusses the self assembly patterns of water droplets and peep into the uniformity both of the size and the distribution mode of water droplets. In former basic experiment, we focus on temperature and the speed of water moisture; generally speaking, higher temperature speeds up the coalescence procedure but does not affects the nucleation size of water droplets in simple plain surroundings; while speed of moisture does affects the nucleation size. As we went farther, deep into convection and found magneto-electric force did play an important role in the self assembly mechanism of water droplets. The topic is mostly concerned as we are surrounded by magneto-electric waves in today’s world. This experiment anchors the first step in discovering the uniformity of water droplets in different environment, and providing insights into the self assembly mechanism of water droplets upon electro-magneto field with nano sizes. 這是一系列關於水蒸氣冷凝為極細微小水珠的實驗。其中可以分為兩大部分； 第一部分是基礎實驗。將水蒸氣導入至潔淨的介面上(蓋玻片)，觀察冷凝水珠的結構。雖然看似簡單平常，但卻有令人驚奇的發現；不同溫度的水蒸氣，其冷凝最初始的細微顆粒之尺寸是相同的 ！爾後隨著溫度的升高，堆疊速率也跟著上升；以致於最後一起呈現出來的水珠大小不一，尺寸不一。 第二部分是將水蒸氣導到磁場及靜電場上，觀察其冷凝結構。這部分的實驗推翻了一般「水分子是電中性在電磁場中不受影響？」的刻板觀念 ！實驗所呈現出來的冷凝水珠，不但於附加磁場中尺寸縮小又不易長大，同時還有固定的自我組成模式( Slef-assembly pattern);而且也發現在磁場中的冷凝小水珠的尺寸比電場中的小，可是電場中的小水珠則表現出較大的均勻特質。
Problems of Safe Storage, Collecting and Recycling of Luminescent Lamps
Our research is connected with the problem of the mercurious wasre products (MWP) recycling, luminescent lamps take a great part among them. The problem of recycling MWP is topical not only in Yakutsk, but also in other cities of Russia because of its toxic influence on the human’s body. Mercury is the top among eight the most dangerous metals. I have analyzed the conditions of recycling of luminescent lamps in Yakutsk schools. I have got data on the problem. There is a great amount of the fused luminescent lamps is stored in the territory of schools, it produces danger for the pupils’ health. On the research work I have proposed some ways of solution of the problem.
天空之城耐震設計與隔震技術之探討
我們的研究包括兩部份，第一部份是實地調查訪問。瞭解地震成因、傷害及現有防震方法，並調查坊間各種建築物類型，及常見私自改變建築物結構現象，做為研究的基礎。第二部份為建築物抗震實驗。研究發現：牆面挖空、頂樓加蓋、樓層挑高，建築物會在該處產生弱點，由此斷裂。柱子數量相同下，散開時支撐力較弱。不對稱建築物遇震時會不自然扭轉且易倒。隔震素材恰當，能有效提高耐震力。滑軌、彈珠隔震效果很好，但位移太大，為實際建築所不容許。建物下加裝阻尼材料，能吸收部份地震能量，降低地震對建築物的危害，並有效控制位移問題，是良好的隔震素材。樓頂加裝消能設施亦能減震，但設計極其不易。 The research includes two parts. In the first part the work is concentrated on on-site visiting and investigation such as understanding the cause of earthquake, the damage and the preventive method currently available, investigating the different types of building and the phenomenon of altering the structure of an existing building without permission by government authorities which is popularly seen in Taiwan. These are considered as the basis of the research. The second part is the experimental study of earthquake resistance of a building. The test results showed that weak-point can be caused at the place where the existing wall is moved or an extra building is attached to the roof or the structure of building has extended space between floors, and fracture always occurs at the weak-point. If the number of columns of a building is the same, then the scattering arranged location of columns is weaker than concentrating type of arrangement of columns. The building having unsymmetrical structure will twist in uneven fashion that causes the building apt to collapse in case of earthquake. Employing proper vibration-absorption material can effectively increase earthquake resistance. Sliding rails and balls can provide satisfiable vibration-isolation effect, but can also cause too much displacement of building structure. Install damping material beneath the building can absorb part of the energy of earthquake, and decrease the damage, and can solve the problem of displacement of building, therefore, damping material can be considered as an ideal vibration-isolation material. Install energy-attenuation equipment can also reduce vibration but the design of the equipment is extremely difficult.
揭開變化球的神秘面紗 --- 探討丘腦至前額葉的路徑連結
During the evolution of humankind, development of frontal cortex has played a critical role, where higher brain function like emotions, self-consciousness, decisions…etc, were all related to frontal cortex. On the other hand, thalamus is usually associated with relaying the sensory signals from peripheral receptors. In order to understand the functional role of frontal cortex, the signal processing mechanisms in the thalamo-frontal cortical pathway became an important research issue. The aim of this experiment was to find a method to dissect a brain slice that contains a connecting route in vitro between thalamus and frontal cortex with functional activity. Through nerve fibers tracking technique using fluorescent-dye (DiI), it was understood that the 3D-space connection between thalamus and frontal surface was an upward curve with a turn of about 110 degrees and bending inwards from the two sides. If a conventional horizontal section was performed, the route would be cut-off and its integrity lost regardless of the direction. To solve this problem, a novel section method was developed to retain the route. Based on the route direction shown by the fluorescent-dye, a piece of brain block was cut and flattened of about 110 degrees. Other sections were performed as control for studying the effectiveness of the sectioning on the plane of the route. Finally, electrophysiological methods were used to verify the connection route was complete and functional. Thalamus-evoked extracellular field potentials in the frontal cortex were observed by changing stimulation strength, adjusting slice temperature and prepared oxygen supply and administration of drugs like CNQX and picrotoxin in the 110 degree flattened slice but not the others. It was found that the reaction was essentially a neuronal response, indicating the pathway between thalamus and frontal surface was retained substantially. With this novel brain slice technique, we can assess the functional connection between thalamus and frontal cortex and investigate the cellular mechanisms of the signal processing in this connection pathway. It is anticipated that present technique provides an important step to further elucidate the functional role of the frontal cortex. 在人類的演化史上，前額葉的發展扮演了極為重要的角色，凡舉情緒、自我意識、決策等，皆與前額葉有關。而丘腦通常與視覺、聽覺及本體感受如痛覺、觸覺、溫度覺的訊息傳遞有關。要了解前額葉的功能，丘腦到前額葉的徑路及訊息處理機制，便成為一個很重要的研究課題。本實驗的目的是尋找一個方法能在離體的腦切片上維持具有丘腦到前額葉連結的徑路並且有功能的活性。經由螢光染料(DiI)神經束追蹤技術，了解從丘腦至前額葉路徑的三度空間連結為一先向下再向上約110度的角度轉折，並同時先由內向外再轉向內的曲線，若用一般水平之切片方法，無論何種方向，其路徑必定會被切斷，不能保持其完整性。為了解決此問題，發展出一種可以保存其路徑的腦切片方法。依照螢光染料所顯示出的的路徑走向，在腦塊的皮質上切一刻痕，將腦塊以110度的角度展平，使其路徑處在一個平面上再切片。最後利用電生理的方法來證明所切出的連結路徑是完整且具有活性。改變在丘腦的刺激強度、調整腦切片(腦脊髓液)溫度、氧氣的供給以及施予藥物CNQX、picrotoxin，觀察其前額葉之電位變化，發現其反應確實為神經反應，表示從丘腦至前額葉的路徑已在這種特殊的腦切片中被完整保存。藉由這個方法，將有助於研究丘腦至前額葉功能性連結，神經網路結構，及其訊息處理機制，並期待以這樣一個全新的方法將來有助於瞭解前額葉的功能。
總站該設在哪裡？—另類費馬點的研究
The definition of "Fermat Point" is that a dot, which lies in a triangle, has the minimum distance to the three apexes. In other words, "Fermat Point" has the minimum distance to three dots which are not on the same line. In the broad sense, then, in a N polygon, a dot which has the minimum distance to the N apexes could be named "Fermat Point." But what if we link up the N apexes and find out that they cannot make a convex polygon? The above is what we wish to fully discuss. Our inspiration comes from a paper on"Fermat Point." It just describes N convex polygon, so we think of putting the case to naturally polygon. The case may be that it is a concave polygon or part of the apexes which lies on the same line. We would not base our study on the conventional methods. Moreover, strictly defined, the repeated line segment will not be taken into account. That is, if the "Fermat Point" drops on the line with more than two dots on it, we just count the\r line segments except for the shorter line segments which were originally included in other studies. According to the theorem, our conclusions are as follows: 1. If N points lie on the same line segment, then the "Fermat Point"can be any point on the line segment. 2. If (N-1) points are on the same line segment, then the "Fermat Point" is on the point which two lines join together. One is that the line segment, and the other is the one which passes the remaining point and\r perpendicular to the first line segment. 3. Now there are (M+N) points. Among them, M points will make a M jog-polygon. The others all drop in the polygon. As the diagram shown beneath, we know that the "Fermat Point" drops on the point which two lines join together. The two lines must pass as many points as possible. 所謂的「費馬點」是指三角形內到三頂點距離和最小的點。換言之，「費馬點」就是到平面上不共線三點距離和最小的點。因此，我們可定義，廣義的「費馬點」即是n 多邊形內到各頂點距離和最小的點，亦即到平面上不共線n 點距離和最小的點，但若平面上n 點不能恰為n 多邊形的頂點呢？這就是我們所要討論的。由於我們的靈感來自一份關於「費馬點」的科展作品，所以我們想到，當平面上n 點不能恰為n 凸多邊形的頂點，甚或其中有一部分的點共線時，將不能以n邊形的方法來探討，但我們可以將之化為m 邊形內（n-m）個點來討論。而更重要的是，我\r 們增加了另一個限制，重複的線段將不被我們列入計算。亦即當所求點落在某一多點共線的線段上時，我們只計算該線段的總長，而不計其中重複的較短線段。根據這個原則，我們試行證明平面上三點、四點、五點及六點的可能情況，期望能從中找出足以推廣至平面上n 點的一般性。結果雖不完美，但我們總算差強人意的歸納出了下列結論：1.若n 點共線段，所求點可為所共線段上任一點。2.若（n-1）點共線段，則由該不共線點引一線與共線段垂直，其交點即為所求。3.若（n+m）個點中有m 個點為一m 多邊形的頂點，另外n 個點落在該m 多邊形內，則由兩個外頂點引直線盡可能通過最多點，該兩直線的交點即為所求。
Isolation, characterization of β-chitin from squid pens and calcium carbonate crystallization on th
Chitin, a polysaccharide common in biocomposites, has an interwoven organic framework that can act as a scaffold for mineralization in natural systems. Acidic amino acids, namely aspartic (Asp) and glutamic (Glu) acids, are the primary active molecules at biomineralisation interphases in mollusks and play an important role in controlling the polymorph and morphology of the associated mineral.\r In this study, chitin was extracted from squid pens and used as a scaffold for crystallization. The chitin scaffold was functionalized with glutamic acid and aspartic acid separately to create an artificial microenvironment for the study of biomineralization of calcium carbonate. The objective was to investigate the usefulness of the extracted chitin to serve as a scaffold for calcium carbonate crystallization, especially the fidelity of the polymorph nucleated to the amino acids and proteins used.