2005年

This research is mainly in observation with Caenorhabditis elegans ’s genetic mutation caused via nervous system abnormal character. In the study, I the sample have been cultivated purified and add some chemical material EMS to speed up C.elegans mutation. Then based on the character to further analysis what causeof gene deal with mutation and observe the effects in heredity. The research has two stages, on the first stage of study the mainly target is to both search and purify the mutation of C.elegans. The second stage is based on the exploration of mutation’s searching andpurifying. Because the certain mutation bodies aren’t easy to find out, the project is still on progress at the beginning of second stage, and we conclude some heredity special cases in preliminary of study. 這個實驗主要是觀察並針對線蟲因為基因的突變所產生的神經系統異常的變異性狀，在實驗中我先將樣品線蟲培養並純化至一定數量，並加入適當藥劑EMS造成其突變，經篩選並分析此性狀，進而找出造成其突變之基因，以及觀察此性狀對遺傳表現所造成的影響。 該計畫分成兩階段，第一階段的實驗重點是在突變株的搜尋以及純化上，第二階段則是在突變基因的探討上，由於特定突變株的搜尋並非容易，所以目前計畫只進展至第二階段的遺傳實驗初期，對於其遺傳特徵與突變形式上已有了初步的分析，但尚未定位出該基因的位置。

隨著工商業發展，環境污染日益嚴重，對多數生長在這塊土地上的人，無疑造成了非常嚴重的影響。但若想要監控目前環境中的空氣品質，則必須具備專業的訓練，及昂貴的實驗設備，對一般民眾而言，根本就做不到。\r 藉由指標植物對所生長環境的高度敏感性，可以發展出一套純天然且免費的環境污染偵測器，不但方便、免攜帶、無須高級儀器協助、更不需要專業的分析技術。為此，我們以對二氧化硫等空氣污染物極為敏感的地衣作為指標植物，對其進行生態與環境污染關係的一系列觀察，並設計相關的實驗，找出環境污染物對地衣的實際影響，使其能夠實際的應用於日常生活，並可加以推廣，讓人人都可以利用地衣來了解自己所處的環境是否遭受污染，為自己家園的環境優劣把關。\r \r 文摘要 :\r With the development of industry and business , environmental pollutions become more and more serious . Undoubtedly , those pollutions have a great effect on us.\r However , by the means of the indicator plant which is highly sensitive to its environment , we can develop a set of natural and free environmental pollution detectors . In this project , we use lichenes,which are very sensitive to air pollution , to do a series of observations and to find out the influence the pollutants have on lichenes . If we can apply this to our regular lives , everyone can use lichenes to see if their environment is polluted or not .

本報告研究內容，是利用電化學氧化還原方法合成金、銀、銅三種奈米粒子，以及探討電流是否會影響電解合成奈米粒子，在前半部成功地利用控制電解的部份條件，如界面活性劑、以及電流值大小，而合成出金、銀、銅三種奈米粒子，利用UV-VIS的光譜分析，鑑定其三種奈米粒子不同的吸收波長，其光譜出現吸收的現象是因為金屬表面特殊的表面電漿共振吸收現象而產生的。但是在本實驗中發現在UV-VIS的光譜中，電壓值的大小對金奈米粒子吸收波長並沒有關係，這些奈米粒子在水溶液中藉由界面活性劑的包覆，而溶解的相當好。 The content of thesis focuses on using electrochemistry oxidation-reduction reaction to synthesis gold, silver, and copper nanoparticles. We confer whether current of the electrolysis is an influence for the synthesis of nanoparticles. We succeed in synthesizing nanoparticle by controlling some terms of the electrolysis, like the micelle concentration, and current value. Using UV-VIS spectrum to analyse wavelength of three kinds of nanoparticles. The special phenomenon of absorption spectra is appeared because the surface plasma resonance on the surface of metal. From the UV-Vis spectra, we didn’t find the exact relationship between the potential value and the absorption spectra of gold nanoparticles. Finally, we also obtained good results in spectra observation, which meant that these nanoparticles encapsulated with surfactants were well solved in the solution.

在這篇報告中，我們以貝斯曲線的做圖原理建立出一種新的曲線－環狀貝斯曲線，進而得到不少有趣的結果。我們發現有名的古典曲線－蚶線，也是屬於二次環狀貝斯曲線。軌跡方程式為：，此時，係數恰符合二項式定理。之後我們推廣至n次環狀貝斯曲線的軌跡方程式：，也符合二項式定理。 在複數平面上，給定z0、z1、z2三點，我們定義出一個二次變換 ，若，，可映射成蚶線的圖形；若z∈實數，則可映射成拋物線。利用此結果類推我們找到一個複數平面上由 z0、z1、...、zn 所決定的n次變換將以原點為圓心的單位圓，映射成n次環狀Bezier曲線。 In this essay, we use the method of forming a Bezier Curve to establish a new curve, circular Bezier Curve, and find a lot of interesting results. We discover the famous classical curve ＂limacon＂, which belongs to the Quadratic Circular Bezier Curve. The locus of Quadratic Circular Bezier Curve is, where. Its coefficients match the binomial theorem. Then we apply it to the locus of nth-circular Bezier Curve:, and it also matches the binomial theorem.On the complex plane, we define a quadratic transformation corresponding to three points—z0,z1 and z2 as .If , where , a limacon is mapped. If z is a real number, a parabola is mapped. With this result, we will find a nth transformation defined by z0、z1、...、zn on the complex plane. It will form a nth-circular Bezier Curve with unit circle centering on the origin.

由於結網性蜘蛛視覺不靈敏，如何在網上藉振動進行獵捕，這是長久以來頗令科學家困惑的難題，當周遭環境各種振源觸網時，首先會產生不同振盪，蜘蛛是否藉由這些振盪得知獵物資訊？如何迅速準確的定位？又有那些決策條件影響蜘蛛的捕獵行為？更特別的，為何蜘蛛在捕獵過程中會“扯網”？本研究以台灣最大型結網性蜘蛛－人面蜘蛛為研究對象，並設計出一套非接觸式的測量方法，就上述謎題作深入的探討後，成功的解開人面蜘蛛的捕獵機制。簡單來說，其機制分為兩大系統：(1)當獵物擾動不明顯，人面蜘蛛會立即扯網，藉有無產生阻尼振盪，以判斷有無獵物存在；在阻尼振盪產生時，蜘蛛將感知其中具有最大阻尼振盪之放射絲為獵物所在方向，而振盪週期長短，係蜘蛛用以判斷獵物遠近之有效因素。(2)當振源明顯時，蜘蛛直接判斷各種擾動的振幅大小、頻率高低、波形模式、振源質量輕重，決定是否啟動捕獵或逃離反應，並在反應前先行定位，亦即以步足腳勾偵測並比較各放射絲之振盪大小，以振盪最大之放射絲為獵物方向，其次藉由第二對步足之位移所產生之準光角，判斷獵物之遠近。蜘蛛正確的將獵物定位後，會以適當的速度往前衝，一口咬住獵物，以蛛絲重重包裹後，拖往網中央並進行吸食。 Giant wood spider, Nephila pilipes, is the biggest orb web spider in Taiwan. The mature N. pilipes may even grow to exceed 5 cm body length. While waiting for the prey, its giant body hangs quietly on the hub of the web. Owing to its ineffective vision and sense of smell, the spider depends almost on detecting the vibration signal of the struggling of web cause by the struggling prey. When various kinds of sources from the environment contact the web, they will generate various types of vibrations which cause the spider to judge whether they represent danger, prey, or irrelevant signals. Our results suggest that if the disturbance is obvious, through discriminating the amplitude and frequency of the vibration, the spider will make a decision whether to attack or escape immediately. Yet, before any decision is made, it will need to locate the source of vibration. For example, it will locate prey correctly by comparing the vibration transmitted from the radiating strings. The radiating strings that transmitted the largest vibration are where the prey is entangled. The displacement of the second pair of legs will generate a quasi visual angle which enables it to comprehend the distance of prey. When the vibration signal is obscure, it will jerk the radiating string immediately. After jerking it, if there is damping oscillation on the web, then the spider can judge the location of the prey. When there is damping oscillation, the radiating string that transmitted the greatest damping oscillation is where the prey is entangled. Furthermore, the frequency of damping oscillation helps the spider to judge the distance of the prey. After locating the prey correctly, N. pilipes approaches the prey fast, wraps it with silk then drags the prey to the hub to feed.\r

目前市面上可以轉換顏色的物品大多可是經由物理變化所控制的，而以化學發光的產品，往往都只能有單一顏色，例如螢光棒，當它被製造出來時，就註定了本身就帶某種顏色，難道光的顏色無法經由某些特殊反應讓其在不同顏色間轉換嗎 ?

完全圖Kn是指一個圖中有n個點，且任意一個點都跟其它的點有邊相連。兩個圖G和H的卡氏乘積G□H的點集V(G□H)＝{(g,h)| g∈V(G),h∈V(H)}，兩個點(g1,h1)和(g2,h2)有邊相連若且為若g1=g2 且h1~h2，或g1~g2且h1=h2。 三個完全圖Ka、Kb、Kc 的立方乘積是指Ka□Kb□Kc。一個圖G中的一點v所連的其它點稱為這個點v的鄰居，也就是N(v)={x | x~v}。一個點集S中的所有點的鄰居的聯集稱為這個點集的鄰居，也就是N(S)=∪v∈S N(v)。如果一個點集S和它的鄰居N(S)包含了一個圖G的所有的點，也就是S∪N(S)＝V(G)稱這個點集S是這個圖G的一個控制集。我們把圖G的所有控制集中點數最少的稱為最小控制集，並定最小控制集的點數為最小控制數γ(G)，也就是γ(G)＝min { | S |, S是G的控制}。 本文的目的在於研究完全圖立方乘積的最小控制，也就是要給γ(Ka□Kb□Kc)一個上界。特別當 a = b = c = n時，γ(Ka□Kb□Kc) = 。 A complete graph Kn is a graph with n vertices, which any vertex is adjacency to every other vertices. The Cartesian product of two graph G and H which is denoted G□H is define as follow: the vertex set V(G□H)＝{(g,h)| g∈V(G),h∈V(H)}，and two vertices (g1,h1) and (g2,h2) is adjacent if and only if g1=g2 and h1~h2 or g1~g2 and h1=h2. The Cartesian product of three complete graph Ka,Kb,Kc is Ka□Kb□Kc,which is the same with (Ka□Kb)□Kc. In a graph G, the neighbor of a vertex v N(v) is the set of the vertices adjacent to the vertex v, that is N(v)={x | x~v}。 The neighbor of a vertex set S is N(S), which is the union of the neighbors of vertex v over S, that is N(S)=∪v∈SN(v). For a graph G, if a vertex set S unions its neighbor N(S) equal to the vertex set of G, that is S∪N(S)＝V(G), we say that S is a dominating set of G. The domination number of a graph G will be denoted as γ(G), which is the minimum size of all dominating set of G.. We give an upper bound to γ(Ka□Kb□Kc). And when a=b=c, γ(Ka□Kb□Kc) ≦

Up to this time we have spent almost three years in studying condensation and water droplets. Little could we have done as compared with the almighty nature. However we are rewarded by the nature as we gradually found the secrets about electro-magneto field and water droplets: The size of water droplets turn smaller upon electro-magneto field and grow more uniformly especially upon electric field. This experiment presented here is actually the diary of the growth of water droplets in condensation, upon magnetic field and electric field. Through convection, it discusses the self assembly patterns of water droplets and peep into the uniformity both of the size and the distribution mode of water droplets. In former basic experiment, we focus on temperature and the speed of water moisture; generally speaking, higher temperature speeds up the coalescence procedure but does not affects the nucleation size of water droplets in simple plain surroundings; while speed of moisture does affects the nucleation size. As we went farther, deep into convection and found magneto-electric force did play an important role in the self assembly mechanism of water droplets. The topic is mostly concerned as we are surrounded by magneto-electric waves in today’s world. This experiment anchors the first step in discovering the uniformity of water droplets in different environment, and providing insights into the self assembly mechanism of water droplets upon electro-magneto field with nano sizes. 這是一系列關於水蒸氣冷凝為極細微小水珠的實驗。其中可以分為兩大部分； 第一部分是基礎實驗。將水蒸氣導入至潔淨的介面上(蓋玻片)，觀察冷凝水珠的結構。雖然看似簡單平常，但卻有令人驚奇的發現；不同溫度的水蒸氣，其冷凝最初始的細微顆粒之尺寸是相同的 ！爾後隨著溫度的升高，堆疊速率也跟著上升；以致於最後一起呈現出來的水珠大小不一，尺寸不一。 第二部分是將水蒸氣導到磁場及靜電場上，觀察其冷凝結構。這部分的實驗推翻了一般「水分子是電中性在電磁場中不受影響？」的刻板觀念 ！實驗所呈現出來的冷凝水珠，不但於附加磁場中尺寸縮小又不易長大，同時還有固定的自我組成模式( Slef-assembly pattern);而且也發現在磁場中的冷凝小水珠的尺寸比電場中的小，可是電場中的小水珠則表現出較大的均勻特質。

利用陰極電弧蒸鍍各種薄膜，如：類鑽膜（DLC）、氮化鈦膜（TiN）、氮化鉻膜（CrN）、氮化鋁鈦膜（TiAlN）以及先披覆上一層氮化鋁鈦膜（TiAlN）再加上類鑽膜（DLC）的合成膜等。這些薄膜現在已經被廣泛的應用於各種刀具、模具的表面處理之中。本研究主要在探討高速鋼鍍上氮化鉻膜（CrN）之後，對於硬度、磨耗性質的改變，以及觀察氮化鉻膜（CrN）表面結構之組織。 在研究中我們運用陰極電弧蒸鍍系統蒸鍍氮化鉻薄膜，分析上運用SEM來觀察薄膜表面結構組織，以及運用洛氏微硬度機來觀察試片的硬度，另外還有使用磨耗試驗機來進行磨耗測試。以上這些測試總括來說都是在得知性質有無實際上的改變，而這些實際上的改變對於蒸鍍之後的模具或刀具都能夠大幅的提高使用的壽命。 We evaporated different kinds of thin films by using the anode of the electronic arc, such as DLC (Diamond-Like Carbon), TiN (Titanium Nitride), CrN (Chromium Nitride), TiAlN (Titanium Aluminum Nitride), and synthetic films of covering TiAlN and DLC. These thin films have been used widely in processing the surface of a variety of cutters and moulds. The purposes of this research were to investigate changes of hardness and abrasion and to observe the organization of the surface structure of CrN after High-speed steel evaporates CrN. In this study, we use the system of the anode of electronic arc to evaporate CrN. Besides, SEM is used to observe the organization of the surface structure of the thin films and Rockwell Micro-hardness Test Machine is used to investigate hardness of testing samples. Moreover, we use Abrasion Tester to test abrasion. These tests are taken to lead to a better understanding whether the quality really changed. These changes of evaporated moulds or cutters would extend their frequency of using.

The study is to investigate Taiwanese tardigrades,and the research of that is few. Tardigrades are commonly called water bears and have been identified more than 750 species. Limno-terrestrial tardigrades are small, 0.2-0.5 mm in length, and mostly found in moss cushions growing on rocks, soil, or the wall of houses. When the environment dehydrates in dry weather, Tardigrades desiccate into a reversible state of metabolic suspension called cryptobiosis. We have been finding a number of tardigrades in moss at many places in Taoyuan country. First, we put the moss on the dissected microscope to seek for tardigrades, and then placed it on the microscope for photographing and observing. The study is mainly focus on Taiwanese tardigrades. we have classified 11 Taiwanese species in four families（Echiniscidae,Calohypsibiidae,Milnesiidae and Macrobiotidae）, making Chinese keys of classification. From the habitat envoriment, the species, the density and the diversity we survey as well as the most suitable pH envoriment we experiment, we approach the relationship between the distribution of tardigrades and their habitat. Besides, we also research lots of conditions which bring cryptobiosis and make culture medium in order to inspect its living. These results indicate that tardigrades desiccate into cryptobiosis in ten munites in acid rain(pH4.65). From outdoor surveys, we have noticed tardigrades can’t be found in the moss right next to road.The length of each family is: Calohypsibiidae＞Milnesiidae Milnesium＞Macrobiotidae＞Echiniscidae. 此研究是探討台灣熊蟲，而有關台灣熊蟲的文獻資料極少。熊蟲屬於緩步門，體長約 0.2-0.5 mm，熊蟲在不利的環境會蟄伏，環境有利時又會膨脹而復甦，而其構造系統不因此而破壞。 我們在桃園縣多處的苔蘚發現熊蟲，我們先將採集的苔蘚放至解剖顯微鏡下尋找熊蟲，再由複式顯微鏡觀察構造並拍照紀錄。研究主要是探討台灣本地的熊蟲，我們已分類出十一種台灣熊蟲，製作中文檢索表。藉由觀測採集環境和所測的密度、歧異度和種類，及實驗出其最適宜的 pH值，探討環境對其分布的影響。也探討各種因素與蟄伏的關係，製作培養基以觀測其生活史。研究結果顯示：1.已經分類出台灣有緩步門四科（端爪科、Calohypsibiidae科、Milnesiidae科和 Macrobiotidae科）十一種熊蟲。2. 污染嚴重或環境髒亂的地方，不會有熊蟲的存在，且熊蟲有群居性。3. 端爪科(棕色)熊蟲在 pH4.65（台灣都會區雨的酸鹼值）以下的液體環境活動力明顯降低。4.熊蟲多分布在高溼度（87.5~90.4％）的地方，不分布在中低溼度 （76％以下） 的地區。5. 各科的體長為 Calohypsibiidae科＞Milnesiidae科 Milnesium屬＞Macrobiotidae科＞端爪科。