吸〝氣〞大法-QCM 對有機氣體之吸附與偵測
中文摘要：\r 化學實驗中，常常會使用到各種具有揮發性的有機溶劑，如醇類、酸類、醛類及酮類等。\r 當吸入過量的揮發性有機溶劑時，將會對人體造成嚴重的傷害，故使用時格外需要留意。有\r 鑑於此，我們開始著手研究如何偵測氣相中的有機氣體分子。\r 本研究中，我們利用聚苯胺薄膜吸附氣體分子的特性並結合對質量變化極靈敏的石英晶\r 體微天平，自行設計了一套簡單、藥品用量少且不需昂貴儀器的實驗系統。藉由此系統，我\r 們有效的針對各種揮發性有機溶劑進行偵測，並進一步地探討不同氣體分子對於聚苯胺薄膜\r 的吸附現象。\r \r 英文摘要：\r Students usually use volatile organic solvent in laboratorys such as alcohols, carboxylic acids,\r aldehydes, ketones, etc. It will injure our body when we breathe in too much volatile organic\r solvent. For this purpose, we begin to study how to detect volatile organic compound in air.\r In this work, we combine polyaniline membrane which can adsorb gas molecules and quartz\r crystal microbalance which is highly sensitive to mass changes to design a simple and inexpensive\r system which needs only little chemicals. With this system, we can effectively detect different\r volatile organic compounds. Furthermore, we can also study the adsorption of polyaniline\r membrance for different volatile organic compounds.
The Flying Chloroplasts Drug Test
Previously, I studied the acute effects of a common broadleaf herbicide, Killex (by Montsano) and a pesticide, Spidercide (by Wilson), on cytoplasmic streaming in detached leaves from the pond weed, Elodea canadensis. I found that Killex, active ingredient 2-4D, an auxin analog, applied at the recommended dose decreased the rate of cytoplasmic streaming by 64% over a 20 minute observational period. The Spidercide, active ingredient d-trans allethrin, a Na+ channel activator, stopped cytoplasmic streaming after a 5 minute exposure and no recovery was observed during the 20 minute observational period.
微陣列基因分析法探討心肌細胞在機械性展延下的基因表現
高血壓所誘發的機械性展延是造成心肌肥大的基本因子，本實驗即藉由微陣列基因分析法同時大量的分析機械性展延所造成心肌細胞的基因表現。將新生鼠的心肌細胞施以 20﹪的機械性展延，抽取其 mRNA製作成 cDNA 探針與現成的 cDNA 晶片進行雜漬反應 （此晶片上包含了 480個如訊息傳遞、控制細胞生長週期、細胞骨架等的已知基因），在眾多有因為機械性展延而造成基因表現差異的基因中，我們選擇了 eNOS 基因（內皮細胞 NO合成?）進行西方墨點法及 NOS活性和 NO 產生量測定的實驗，進一步證實 eNOS 的基因表現量的確是增加的，此一結果與微陣列基因分析所得之結果不謀而合。 Mechanical stretch induced by high blood pressure is an initial factor laeding to cardiac\r hypertrophy. The use of cDNA microarrays has made it possible to simultaneously analyze\r stretch-induced gene expression in cardiomyocytes. Neonatal rat cardiomyocytes were cultured on\r malleable silicone dishes and were stretched by 20%. We compared the transcript profiles of\r cardiomyocytes under mechanical stretch for 60 minutes by hybridization of cell-derived cDNA to\r DNA probes immobilized on microarrays. The microarrays contained probes for 480 known genes\r including signal transduction, cell cycle regulators, cytoskeleton and cell motility, and so on. Eighteen\r genes were indentified that showed significantly differential expression in response to mechanical\r stretch in cardiomyocytes. Of the represented genes expressed, endothelial nitric oxide synthase (eNOS)\r genes was the most interesting one. Northern blot and western blot analysis further quantified the\r expression of eNOS gene. Mechanical stretch also increased constitutive NOS activity and NO\r production. Our results indicate that mechanical stretch induces eNOS gene expression thus increases\r constitutive NOS activity and NO production in cardiomyocytes.
光子晶體合成、特性與應用
We report an investigation on the synthesis, characterization, and application of photonic crystal. In the study of the synthesis of SiO? nanoparticles for the building blocks of photonic crystal, it is found that by changing the concentration of NH3 solution, we are able to control the size of SiO? nanoparticles. After trying several different methods, we discover that the vertical substrate method is the best way to arrange nanoparticles into a periodic structure. From scanning electron microscope, we confirm that SiO? nanoparticles can form a three dimensional hexagonal photonic crystal. From transmission experiment, we find that the wavelength of the minimum transmission is proportional to the size of nanoparticles. This result implies that using photonic crystals we can control the behavior of electromagnetic wave. Finally, we fabricate CdS nanoparticles on the top of photonic crystals with different diameter of SiO? nanoparticles. Using photoluminescence measurements, we show that by controlling the lattice constant of a photonic crystal the luminescent efficiency of CdS nanoparticles can be substantially enhanced. Out results, therefore demonstrate that photonic crystals are very important for the application of light emitting devices. 本研究主要是著重於探討光子晶體合成、特性分析及其應用。在有關合成光子晶體之奈米二氧化矽顆粒方面， 發現在合成過程中利用氨水的溶量可以控制顆粒的大小。在將奈米顆粒排列成光子晶體的研究中， 嘗試了多種方法後， 發現垂直基座法為最快速有效的方法。從掃瞄電子顯微鏡的觀測， 證實奈米顆粒是以六角對稱排列成整齊的光子晶體。在光子晶體的特性分析中， 利用光穿透實驗， 發現電磁波穿透率最小的波長與奈米顆粒成正比關係， 這顯示出可以利用光子晶體來控制光的行為。最後，本研究將光子晶體與硫化鎘奈米顆粒結合，經由光激螢光譜， 證明光子晶體確實可以增進物體之發光效率,這對發光元件的應用， 將有很大的幫助， 可以節省大量的能源
雞胰臟去氧核醣核酸水解？多型性之探討
去氧核糖核酸水解?，(deoxyribonuclease,簡稱DNase)為一種核酸內切?。目前研究得最透徹的去氧核糖核酸水解?為牛胰臟的DNase I，有四種以上的同功?存在。而牛和雞的DNA序列有極高的相似度，由之前的研究中在等電點焦集電泳法顯示雞胰臟DNase I 具A、B 兩種同功?，本實驗則希望能進一步了解兩種同功?的差異原因。在抽取RNA 後，以RT-PCR 方法合成cDNA，將其以限制?切割，再以大腸桿菌作為載體進行轉殖及繁殖培養，最後將其DNA 定序後進行分析比較。目前已完成第一次定序，但因引子接合過程中有部分位置錯亂造成殖體無法進行表現。另外在此次所得之序列中有發現一個胺基酸的轉變，而其是否為多型性之表現則有待進一步的實驗證實。The most advanced research on deoxyribonuclease in current states is on the bovine pancreatic DNase I, more than four of which isoforms have been found. It is shown that the DNA sequences of chicken and bovine have relatively high similarity. In addition, according to the result of isoelectric focusing from previous researches, chicken pancreatic DNaseI has two isoforms (A and B.) In this research, the author expects to establish more knowledge on the differences on the isoforms and the causes. After receiving the RNA, the RT-PCR is preformed to incorporate the cDNA, which is later cleaved by restriction enzyme and inserted into the plasmid DNA of the E. coli host cell to be cloned. So far a polynucleotide sequence has been deduced from clones of the cDNA, but it cannot be expressed successfully in E.coli due to few random mistaken splicing. There is an alternation of one amino acid, and whether it is the actual state of isoform or not still requires further proof.
海格的爆尾釘蝦－鹼性電池的探討
鹼性電池使用的電解液均為強鹼，電池中的鋅極會與其發生腐蝕反應，使得電池放電壽命降低，並產生氫氣，而大量氫氣使得電池有爆裂的危險，為了改善上述問題，我們自行設計了氣體觀測儀器，用來檢測銀鋅電池充放電與靜置時之氣體產生量，更藉由探討影響變因的過程找出銀鋅電池較合適之使用條件，其中包含電解液種類及濃度之選擇、電極面積與充電電流對使用效果之影響，並改善電池腐蝕程度；我們將鋅極以浸鍍處理(Sn:Pb ＝1:1 )並搭配電解液添加物(KOH:Zn(OH)42 －＝ 2:1) 可效率地抑制氣體產生；更用氯化銀粉末取代傳統氧化銀極片為正極，發現氯化銀可代替氧化銀電極，且有不錯的放電效果。;As we know, the electrolyte solution used in an alkalinebattery is a concentrated alkaline solution, which corrodes the zinc electrode in a battery of this kind. The corrosive reaction not only reduces the lifespan of the battery but also produces hydrogen, causing the battery to explode. This study is intended to help us design a device to measure the quantity of hydrogen gas generated in the charging of a zinc-silver battery. We have studied several factors affecting zinc-silver battery, including the variety of electrolyte, the concentration of alkaline solution, the surface of electrode, and the density of charging current, etc. To reduce the corrosion of zinc electrode, we plate the zinc electrode by the immersion electroless plating method, using several kinds of low-polluting anti-corrosive additives (metallic compounds such as lead and tin). We also add zincate ion into electrolyte solution to further reduce the quantity of hydrogen produced. The experiments show a zinc-silver alkaline battery works most efficiently, if the powder of silver chloride is used instead of silver oxide.
無孤力點無交錯分割的區塊細分及五個新的Riordan組合結構
將一個集合{1,2,...,n}分成數個非空的集合（組，區塊），稱為此集合的一個分割。如果可以找到1 ≦ a 已知無孤立點無交錯分割以Riordan 數{rn}n≥0 =1,0,1,1,3,6,15,36,... 來計數。在這篇文章中我們研究無孤立點無交錯分割的一些性質。
首先我們考慮無孤立點的無交錯分割按區塊的細分。我們得出：集合{1,2,...,n}恰含k個區塊的無孤立點的無交錯分割的個數為：
其次，我們證明bn,k和多邊形的剖分有令人訝異的關連。令dn,k是用不相交對角線將凸n 邊形分成k 塊的方法。我們用代數方法證出 bn,k = dn+2−k ,k，也給了一個新的組合證明。
最後，透過對應的方法，我們找出了七個嶄新的組合結構，這些結構都是以Riordan 數來計數。
Partition the set {1,2,...,n} into several nonempty sets (blocks) and call it a partition. If there exists 1 ≦ a It is known that the nonsingleton noncrossing partitions are counted by Riordan numbers {rn}n≥0 =1,0,1,1,3,6,15,36,... In this paper we study the properties of them.
First we consider the enumeration of nonsingleton noncrossing partitions in respect to the blocks. We prove that the number of nonsingleton noncrossing partitions of {1,2,...,n} with k blocks is
Then we give a connection between nonsingleton noncrossing partitions and polygon dissections. Let dn,k be the ways to dissect an n –gon with noncrossing diagonals. We prove that bn,k = dn+2−k ,k
We also give a combinatorial proof. Furthermore, by way of the technic of bijection, we find 7 new combinatorial structures counted by Riordan numbers.