光基因新角色-cop8
COP8 is the second unit of COP9 signalsome. In the comparison of BLAST ,we found that\r LLPC14 cDNA and the protein sequence are quit similar to the COP8 of Arabidopsis thaliana\r Therefore, we are interested in the role that COP8 plays in the photomorphogenesis and try to find\r the length of the sequence. So far, no result is obtained about the 3’-RACE, but we’re surprised to\r find that the sequence found in 5’-RACE is shorter than what we’re know, which suggests the RNA\r length of the cDNA used in the RACE combination has decreased.\r Although the final results of the COP8 5’and 3’-RACE haven’t been achieved, it is sure the\r COP8 is highly-conserved in many species. Since the similarity between LLPC14 and the COP8 of\r Arabidopsis thaliana is as high as 90 percent, we used the COP8 of Arabidopsis thaliana to detect the\r COP8 protein in pollen.在過去種綠豆芽的經驗中,不難發現它本身因為環境的不同,而有不同型態白化\r 的現象,例如:黑暗下萌發的豆苗。但尚未深入去理解白化的原因;而在後來的種植\r 經驗上,也發現有許多令自己覺得困惑的相同處。\r 在高二的生命科學第四章中,有提到光照與黑暗生長的植株,在構造比較上有明\r 顯的不同;其中,提及植物的活性組織存在「光敏素」(phytochrome),此色素蛋白的\r 存在於光照後,會引發一連串反應,至此,也稍微了解植物的萌發反應的差別。\r 故藉由個人在中研院植物所的資優生培訓過程,便以此為前題,進一步探究植物基\r 因組COP 8 與白化現象的相關性