全國中小學科展

2022年

水熊蟲於化學環境壓力耐受機制探討Tolerance mechanisms of Tardigrade under chemical environmental stresses

常見模式生物檢測化學環境壓力後無法重複使用。本研究探討對環境變化敏感的大生熊蟲抗化學環境壓力機制,評估其作為重複使用模式生物可行性。得知多數大生熊蟲於硝酸鹽、酸鹼值改變與殺蟲劑環境壓力下仍能活動或隱生。以亞甲藍簡易染色可確認化學環境壓力下大生熊蟲是否受傷害,如體表角質層被破壞會導致完全染色。分析大生熊蟲於常見硝酸鹽環境壓力下體內脂質含量、總蛋白質單體表現量與總抗氧化能力,推測出大生熊蟲對抗硝酸鹽環境壓力機制:1.藉由> 20 kDa持續性活化蛋白抗硝酸鹽環境壓力。2.活動大生熊蟲增加體內脂質含量隔絕環境硝酸鹽。3.隱生大生熊蟲以抗氧化系統降低硝酸鹽產生的氧化壓力傷害。以上結果得知大生熊蟲具潛能檢測常見化學環境壓力並作為重複檢測之模式生物。未來將持續評估大生熊蟲可檢測的化學環境壓力並為人類健康把關。

Investigating the application of nanotechnology for detecting fishes hatching time

Introduction: Using advanced technologies such as nanotechnology in the food and fishery industry, as one of the most important industrial sectors of countries, has received too much attention. Traditionally, fishing and hunting have been considered important sources of supplying food. The subject and methodology: The study aims to investigate nanotechnology for detecting fish hatching time. This is a review article that collects the information from databases such as Sid, civilica, and Google Scholar. In the end, 22 papers were studied for extracting and collecting the required information from the abovementioned scientific database. Finding: After examining the food, drug, and agricultural-related papers published from 2009 to 2020, it was concluded that small Nano-sensors, controlling & monitoring systems made from nanotechnology can be installed on fishing nets, fishing rods, and other fishing equipment. These devices (Nano-sensors and controlling & monitoring systems) will help fishes so that they don’t get caught. In this way, as a fish gets close to the fishing equipment, it will receive sound, smell, or heat-based alarm. Therefore, the fish will stay away from the fishing equipment. The result: according to the finding of this study, it can be concluded that excessive fishing in the hatching time will be avoided by the application of nanotechnology in the fishing equipment. As a result, the following advantages will be secured: 1- There are lots of opportunists who misuse fish during the hatching time. With the application of nanotechnology, they will be stopped. 2- Opportunists are ambushing in different time points to misuse fish. Also, the guards might be ignorant. With the application of nanotechnology, guards are no longer required. 3- This plant is cost-effective too.

Expectations for extension of cell life and next generation anticancer drugs by using secondary metabolites of actinomycetes

Inhibitory effects of the secondary metabolite of actinomycete were examined on cell cycle of the yeasts of S. pombe and S. cerevisiae. The secondary metabolite was obtained from cultivation of the actinomycete isolated from the soil of Owakudani in Hakone, Japan. The fifth fraction of the secondary metabolite by ODS column separation (HK-T5), which was soluble to pure methanol, was used in the present experiments. The HK-T5 brought about the delay of forming colonies of S. pombe for about 11 days compared to that cultivated without the HK-T5. The delay of the colony formation was longer for the S. pombe cultivated with more amount of the HK-T5. The cultivation with HK-T5 also brought about the extension of the lifespan of the S. pombe for more than 10 weeks in a liquidus medium. The cell life recovered the ordinary manner by removal of the HK-T5, meaning that the activities of the HK-T5 is reversible. These facts confirm the suppression of cell cycle, and the delay of cell growth by the HK-T5. These phenomena were similarly observed for S. cerevisiae. Comparison of the action of HK-T5 with hydroxyurea, which is an anticancer drug inhibiting the cell cycle at S phase, clarified that the inhibitory action of HK-T5 worked at the phase earlier than S phase. The combined effects of HK-T5 on the cell cycle were evaluated with triamcinolone acetonide (TA), or aspirin, the former of which is a drug synchronizing cancer cells in S phase, and the latter keeping human cells in G1/G0 phases. The combined use of HK-T5 with TA synchronized the cells at the phase slightly proceeding from G1 to S phase without toxicity. On the other hand, the combined use with aspirin made the inhibitory effect of HK-T5 inactive. Hence, the HK-T5 is attractive as a drug for the extension of cell lifespan, and anticancer therapy.

Expectations for extension of cell life and next generation anticancer drugs by using secondary metabolites of actinomycetes

Inhibitory effects of the secondary metabolite of actinomycete were examined on cell cycle of the yeasts of S. pombe and S. cerevisiae. The secondary metabolite was obtained from cultivation of the actinomycete isolated from the soil of Owakudani in Hakone, Japan. The fifth fraction of the secondary metabolite by ODS column separation (HK-T5), which was soluble to pure methanol, was used in the present experiments. The HK-T5 brought about the delay of forming colonies of S. pombe for about 11 days compared to that cultivated without the HK-T5. The delay of the colony formation was longer for the S. pombe cultivated with more amount of the HK-T5. The cultivation with HK-T5 also brought about the extension of the lifespan of the S. pombe for more than 10 weeks in a liquidus medium. The cell life recovered the ordinary manner by removal of the HK-T5, meaning that the activities of the HK-T5 is reversible. These facts confirm the suppression of cell cycle, and the delay of cell growth by the HK-T5. These phenomena were similarly observed for S. cerevisiae. Comparison of the action of HK-T5 with hydroxyurea, which is an anticancer drug inhibiting the cell cycle at S phase, clarified that the inhibitory action of HK-T5 worked at the phase earlier than S phase. The combined effects of HK-T5 on the cell cycle were evaluated with triamcinolone acetonide (TA), or aspirin, the former of which is a drug synchronizing cancer cells in S phase, and the latter keeping human cells in G1/G0 phases. The combined use of HK-T5 with TA synchronized the cells at the phase slightly proceeding from G1 to S phase without toxicity. On the other hand, the combined use with aspirin made the inhibitory effect of HK-T5 inactive. Hence, the HK-T5 is attractive as a drug for the extension of cell lifespan, and anticancer therapy.

IoT based automatic water temperature adjustor

This paper represents IOT Based Automatic Water Temperature Adjustor. IoT (Internet of Things) refers to the network of physical objects that are embedded with sensors, software, and other technologies for the purpose of connecting and exchanging data with other devices and systems over the internet. This system is for adjusting water temperature according to the possible surroundings such as home temperature, atmosphere temperature, etc. To solve problems like high water temperature while using, time-consuming waiting for water to heat and cool, high power consumption, and not satisfying water temperature this system offers the feature for automatically adjusting the temperature. Arduino, DHT11 (Temperature-Humidity Sensor), Bread Board, DS18B20 (Water Temperature Sensor), Jumper Wires, Resistor, I2C OLED, Water Heating Coil, Relay and LED are used for operating this system. The application of this system is very vast as it can be implemented in power plants, hospitals, mountain regions, local homes, and lodges. This system is time-saving, cost-efficient, easy to implement, provide automatic features, less power consumption, safety, and many more. Compared to other water geyser systems it has the feature of automatically detecting the environmental temperature and adjusting the temperature of the water accordingly. This system is still in its developing phase.

Development of an Android Application for Triage Prediction in Hospital Emergency Departments

Triage is the process by which nurses manage hospital emergency departments by assigning patients varying degrees of urgency. While triage algorithms such as the Emergency Severity Index (ESI) have been standardized worldwide, many of them are highly inconsistent, which could endanger the lives of thousands of patients. One way to improve on nurses’ accuracy is to use machine learning models (ML), which can learn from past data to make predictions. We tested six ML models: random forest, XGBoost, logistic regression, support vector machines, k-nearest neighbors, and multilayer perceptron. These models were tasked with predicting whether a patient would be admitted to the intensive care unit (ICU), another unit in the hospital, or be discharged. After training on data from more than 30,000 patients and testing using 10-fold cross-validation, we found that all six models outperformed ESI. Of the six, the random forest model achieved the highest average accuracy in predicting both ICU admission (81% vs. 69% using ESI; p<0.001) and hospitalization (75% vs. 57%; p<0.001). These models were then added to an Android application, which would accept patient data, predict their triage, and then add them to a priority-ordered waiting list. This approach may offer significant advantages over conventional triage: mainly, it has a higher accuracy than nurses and returns predictions instantaneously. It could also stand-in for triage nurses entirely in disasters, where medical personnel must deal with a large influx of patients in a short amount of time.

Investigating the application of nanotechnology for detecting fishes hatching time

Introduction: Using advanced technologies such as nanotechnology in the food and fishery industry, as one of the most important industrial sectors of countries, has received too much attention. Traditionally, fishing and hunting have been considered important sources of supplying food. The subject and methodology: The study aims to investigate nanotechnology for detecting fish hatching time. This is a review article that collects the information from databases such as Sid, civilica, and Google Scholar. In the end, 22 papers were studied for extracting and collecting the required information from the abovementioned scientific database. Finding: After examining the food, drug, and agricultural-related papers published from 2009 to 2020, it was concluded that small Nano-sensors, controlling & monitoring systems made from nanotechnology can be installed on fishing nets, fishing rods, and other fishing equipment. These devices (Nano-sensors and controlling & monitoring systems) will help fishes so that they don’t get caught. In this way, as a fish gets close to the fishing equipment, it will receive sound, smell, or heat-based alarm. Therefore, the fish will stay away from the fishing equipment. The result: according to the finding of this study, it can be concluded that excessive fishing in the hatching time will be avoided by the application of nanotechnology in the fishing equipment. As a result, the following advantages will be secured: 1- There are lots of opportunists who misuse fish during the hatching time. With the application of nanotechnology, they will be stopped. 2- Opportunists are ambushing in different time points to misuse fish. Also, the guards might be ignorant. With the application of nanotechnology, guards are no longer required. 3- This plant is cost-effective too.

Prismalla: Mist water collector

The lack of drinking water in human settlements triggers a series of problems that are linked and affect the development of humanity: health problems, lack of water security for companies, lack of jobs, insecurity, among others. We observe this problem in the communities of the municipality of Las Vigas de Ramírez, Veracruz, where there is a great problem with the water supply, although there is a high presence of mist. Faced with this situation, we undertook the task of investigating a water harvesting method that is easy to implement, operate and maintain. We investigated and analyzed the methods of mist condensation through physical barriers, finding that the polyethylene shadow mesh was the means to achieve this, because it allows the passage of the wind, it is very light, easy to manipulate and above all that it presents the phenomenon of percolation that allows water droplets of various diameters to be accommodated therein. We designed a device that allows to present a mist catchment area through a prismatic structure enabled with meshes and condensed water receivers, portable, easy to use and maintenance and very economical with a performance of 20 liters per day. To achieve our project, factors such as air humidity, dew point, wind speed and direction, height, temperatures and available spaces must be considered.

A New Method For Microplastic Removal and Optical Measurement

Microplastics are tiny invisible plastic pieces that are piling up in the marine environment emerging as one of the many environmental issues which our planet is facing today. Researches for the removal of these particles are important because studies that have been made so far haven't come up with an effective solution. This project aimed to detect microplastics and remove them from aqueous environments with an effective and practical method then it was aimed to determine the removal amount of microplastics by optical measurements with the developed system. Firstly, the magnetic carbonanotubes (m-CNT) which is intended to hold onto the surfaces of microplastics was synthesized and added to the mixture of microplastics. Then the magnet within a glass tube was passed through the mixture and the sample was cleared of microplastics. A spectrometer was made to monitor this process and after its calibration, it was used to measure coffees with different concentrations. It has been shown that their concentrations can be determined by calculating the transmission values and Rayleigh scattering. In the end, it has shown that there are no micro or nano-sized plastic particles when removed with M-CNT, within the accountable range of the spectrometer that had been made. Hence the removal of the microplastics: an invisible threat for the environment has been studied by combining nanomaterials with unique surface properties in the removal process and an optical principle such as Rayleigh scattering, a new technique has been developed that can measure quickly, economically,

彩色二維條碼手持產品開發之探討

QR Code是由黑白模組組成的二維數位條碼,掃描後可讀取儲存的訊息。受限於設計原理,QR Code使用二進位制儲存資料。增加模組數目可增加資料量,但若在條碼內塞進太多模組時,尺寸太小的模組將無法被掃描器讀取。此外,目前QR Code掃描器僅支援單張掃描,並無法應付同時多張條碼掃描的實務需求。 如能克服顏色辨識,理論上彩色二維條碼將能克服現行QR Code的限制,但市面上並無相關產品可供測試。因此本專題設計了一款10×10、具8顏色的"Colour Matrix",並利用Raspberry Pi開發Colour Matrix在手持裝置上運作的軟硬體來進行實驗。此實驗成功利用機器學習演算法在Raspberry Pi上進行的顏色辨識。開發的程式在單張掃描上效能與使用pyzbar辨識QR Code相當;在多張掃描方面,使用pyzbar辨識QR Code的解碼成功率為3.1%,而本專題的方法將成功率提升至92.4%,擴增數位條碼的使用範圍,具商用價值。