全國中小學科展

2018年

Graphene Nanoplatelet-Embedded Acrylic Paint for Low Cost Waterproof Paintable Capacitive Sensors and Free Standing Supercapacitors

Modern capacitive touch input and proximity sensing technologies are rigid and limited to flat substrates making it impossible to apply them onto objects with irregular geometries like textiles or car handles. Furthermore, the high cost restricts the applications to small surfaces and cannot be scaled up to be applied on large surfaces such as walls. Therefore, a paint-on scheme would broaden the applications of capacitive touch input and proximity sensing devices. Paintable capacitive sensors are an emerging technology hindered by the high cost and lackluster properties of conductive paints. Existing conductive paints utilize expensive filler materials such as silver and gold to achieve high conductivity but suffer from low surface area. High surface area is critical for capacitive proximity sensors to detect objects from far distances and for overall sensitivity. Carbonaceous alternatives using micronized graphite exhibit low conductivity, require high loadings and most disintegrate when in contact with water. Multilayer graphene nanoplatelets are investigated for their high conductivity, high surface area, low cost, flexibility and eco friendliness. A waterproof acrylic latex is combined with multilayer graphene and dispersed via bath sonication. The optimal time of sonication and optimal graphene loading is determined through systematic testing. An Arduino Uno is loaded with a CapSense library and the graphene based paint is utilized as the interface to sense both touch and proximity.

Development of Models for Performance Index (PI) and Score Index(SI) of Individual players based on 5 European Soccer Leagues

Most football managers are not aware of the need for analysis of soccer data, which is one of the dynamic sports. In this study, we developed a statistical model with performance indicators and score indicators of individual soccer player based on various event data including dynamic features such as goal, assist, pass, etc. In this study, the correlation between the dependent variables and the explanatory variables, and each explanatory variable was confirmed through the correlation analysis to solve the problem of multiple communicability from the regression model and to analyze the statistically significant preliminary model. In addition, we analyzed the correlation between individual rating of the players and the data recorded in the soccer games, and found that there has been a problem with the rating of the soccer players evaluated by the reporters and the soccer statistics site. To solve this problem, we developed a model that best fit the performance indicators of individual soccer player using the linear regression model and the beta regression model. The performance index model of the athletes was developed by comparing the R-squared value and the mean absolute percentage error of two models, the linear regression model and the beta regression model, and found out the beta regression model is better model to use. By using the estimated regression coefficients of the regression model we made new PI model. Score Index, which is the attractive point of soccer, was developed by comparing Poisson regression model and negative binomial regression model based on AIC value, and the one using negative binomial regression model was found to be better. Through the model developed by this study, it is possible to collect the event data recorded by individual athletes for each soccer game, and obtain the PI & SI index which are the athlete performance index models. This allows us to evaluate each team's players objectively, analyze the team's deficiencies, and provide tools to find players, who can fill in the missing positions of the teams. This study can also be utilized to grasp the performance of athlete in real time by simulating the resultative model.

Random number generators and their applications in Computer Science with the Monte Carlo Method

Monte Carlo methods are non-parametric algorithms that use random numbers and theorems of probability theory to approximate values that are not random. The purpose of my research was to approximate the surface of different geographical areas that can be easily approximated to polygons (e.g. lakes, glaciers, deserts) with Monte Carlo simulations starting from either Cartesian coordinates or pictures. Computer science would not exist without math, and this research project showed me the importance of a deep understanding of probability theory in the world of simulations and, more generally, the importance of developing new theorems and algorithms. The results of my research could be developed in different ways: it would be interesting to produce software that allows one to approximate areas from pictures taken from a smartphone; as well, the theorem I found has to be proven, and also Monte Carlo methods as a means of random number generation can always be improved. There are still many possibilities.

表格塗色遊戲之分析

這份研究所探討的主題源自於1976年USAMO第一大題:將一4×7矩形方格表的每格塗色黑色或白色,欲使所有能構成矩形頂點的四個方格皆不全為同色。試證明其塗色必定失敗、或給出滿足的塗色方式。此研究從上述題目延伸,增加可填入的顏色數量、改變方格表的長寬,甚至將方格表改為三角格子表。研究過程主要運用鴿籠原理、組合數量之計算及柯西不等式來分析。我們已幾乎完整討論完矩形方格表中填入2色、3色,及三角格子表中填入2色的所有情況;並且對於矩形方格表,我們找到了一條判別式,可以判斷一般化的某情況下塗色是否必定失敗,但有部分必定塗色失敗的情況無法由此判別式判斷,需藉由其他方式討論。此外,我們也嘗試從滿足的塗色方式中找尋規律並建立構造的規則。

味噌萃取物Soyasaponin I 抑制肺癌細胞轉移並且減少細胞激素分泌及抑制惡體質進展

研究指出Soyasaponin I 是味噌中的一種成分,其效果可以抗發炎,因此,本實驗想使用Soyasaponin I 探討其是否能應用於肺癌治療,及其對肺癌的轉移及癌症引起惡體質影響。 方式:使用肺癌細胞A549和H1299及正常的肺細胞HEL299進行WST-8試驗,測試Soyasaponin I 對三種細胞的存活率影響,用螢光試驗及免疫染色確認死亡方式,用Western blot及免疫螢光染色測試Soyasaponin I 對p-NF-κB入核的效果,進行boyden chamber模型測試Soyasaponin I 對於A549和H1299的侵犯速度的作用,並使用ELISA測出癌症引起惡體質的細胞分泌的IL-6細胞激素濃度。為了研究Soyasaponin I對於cachexia (惡體質)的影響,我們以人類脂肪組織利用原始培養出脂肪細胞,加入Soyasaponin I於脂肪細胞,以免疫染色研究。 成果:在細胞存活實驗發現高濃度Soyasaponin I 的濃度以細胞自噬引發癌細胞死亡,且不傷害正常肺部細胞,另外低濃度的Soyasaponin I 則使兩種肺癌細胞的突觸皆縮短及細胞變圓形;用Western blotting 證明Soyasaponin I 治療後mesenchymal的標記 (N-cadherin)明顯減少,而epithelial 的標記 (E-cadherin)明顯增加,接著在boyden chamber模型發現Soyasaponin I 會引發肺癌細胞發生間質-上皮細胞轉型,轉換成爬行較慢的上皮細胞型細胞; Soyasaponin I 減少肺癌細胞IL-6的分泌,抑制發炎。用免疫組織染色證明Soyasaponin I 治療後,原始培養脂肪細胞的惡體質標記 (UCP-1)明顯減少,減少棕色脂肪細胞形成,減少能量消耗及惡體質發生。

CHST11基因表現量對於肺癌細胞產生上皮間質轉化(EMT)之影響 The Expression of CHST11 Controls Epithelial-to-Mesenchymal Transition in lung cancer cells

肺癌的轉移是治療失敗的主因之一,因此了解癌細胞轉移的機制對於治療肺癌極為重要。上皮間質轉化(EMT)是癌細胞轉移的起始,且代謝體異常更是影響肺癌細胞EMT的關鍵,然而相關機制仍不明確。本研究對肺腺癌細胞EMT前後的基因體學資料(GSE49644),以及臨床上246位肺腺癌病人的EMT狀態(GSE31210),分析其關聯性以及預後指標意義。根據分析結果,並進一步查閱相關文獻後發現CHST11在癌症中角色極不明確,因此,我們針對CHST11基因進行進一步的研究,探討CHST11表現量與肺癌細胞發生EMT的關係以及其可能機制。 根據實驗結果,本研究發現:一、葡萄胺聚糖生合成路徑與非小細胞肺癌的 EMT高度相關; 二、CHST11- SNAI2路徑可調控肺癌細胞EMT過程; 三、 CHST11可做為肺癌病人的不良預後指標。這些結果或許能幫助我們更了解在肺癌細胞的代謝體異常對於肺癌細胞EMT過程中的作用。

Novel Biotechnological Approach for Recognition and Purification of Antibody: Lectin Affinity Membranes

Immunoglobulin G is a glycoprotein structured molecule that is produced by the immune system and protects organism from harmful effects of antigens. Ig G amount in the blood plasma is an appropriate indicator of; infection, cancer, diabetes, cardiovascular diseases, Alzheimer and other autoimmune diseases. Besides, purification of Ig G used in the treatment of these diseases from naturel sources is carried out at high costs on the World market. It is hard to obtain Ig G in high amounts and without any decomposes, that’s why it is important to develop new systems that will help to recognize and purify Ig G antibody. In this project, my purpose was; recognizing Ig G antibody with efficient, high amounted, fast, easily, with less toxicity, economically and purifying Ig G in high ratios from its natural sources. For this purpose p(HEMA-EDMA) membranes are synthesized with free radical photo polymerization method and characterized according to SEM images, swelling behaviors FTIR analysis and elemental analysis. In order to adsorb Ig G to polymeric membranes; polymeric membranes are activated with silanization agent (IMEO) and derivatized with Con A which is a lectin affinity ligand. In the SEM results it is examined that membranes are in spherical structures. Highest swelling value is determined as 224.8%.Binding of IMEO was demonstrated with FTIR and Elemental Analysis. Optimum conditions for Ig G adsorption to membranes are; 1.5 mg/ml initial Ig G concentration, 30 minutes of adsorption time, pH 4 citrate buffer 37 0C and without any different ion strength. Optimum adsorption capacity is determined as 253.8 mg/cm2 and it is also determined that this value is 7 times higher than nonspecific Ig G adsorption to p(HEMA-EDMA) membranes. Ig G adsorption-desorption cycles (5 times) proved that product is reusable without losing its adsorption capacity. According to the electrophoresis, Ig G could be desorbed in pure form without any denaturation to its structure.

New approach to the synthesis of functionalized fluoroalkenes

Fluorine has a big influence on physical, chemical and biological properties of organic structures. Organofluorine compounds are widely used in modern medical chemistry to develop new drugs. Insertion of fluorine atom into organic molecules can improve their reactivity in biological systems, increase their metabolic stability, lipophilicity and permeability through membranes. As a consequence, in recent years, the percentage of drugs containing one or more fluorine atoms has increased rapidly up to 40%. The fluoroallylic fragment is also able to change properties of bioactive molecules. Its introduction into such structures as inhibitors of histonedeacetylase, inhibitors of matrix metalloproteinase, asparagine, glutamine, etc. increases their biological activity and electronic properties. We propose a new method for the synthesis of functionalized fluoroalkenes, based on the generation of fluoroallyl nucleophiles from silyl- and boronyl-substituted fluorocyclopropanes and their further usage in the allylation of carbonyl compounds or their derivatives. Due to the fact that the cyclopropanation of alkenyl boronates is not possible under conditions of alkaline dehydrohalogenation of dibromofluoromethane, we have developed a new method for the preparation of silyl- and boronyl-substituted cyclopropanes, which consist of carbene cyclopropanation of multiple C=C bonds by sodium dibromofluoroacetate catalyzed by (IPr)AgCl. The new method is effective for the cyclopropanation of not only boronyl- and silyl-substituted olefins, but also for low-reactivity alkenes, such as monoalkyl substituted alkenes, allyl alcohol ethers and α,β-unsaturated carbonyl compounds. The conditions for isomerization of silyl- and boronyl-substituted fluorohalocyclopropanes in the presence of catalytic amounts of copper (I) bromide in acetonitrile was selected. It was shown that the regioselectivity of the process is determined by the thermodynamic control. Thus, the formation of fluorovinylsilanes or fluorovinylboranes in the isomerization of α-silyl- or α-boronyl-gem-bromofluorophenylcyclopropanes and fluoroallylsilanes upon isomerization of β-silyl-gem-bromofluorophenylcyclopropanes was observed. Thus, new types of fluorinated reagents were obtained that are not previously described in the literature (...)

世界向你招手:文化智商、個人因素與國際地理知識之關係探討

本研究的目的為探討文化智商(CQ)、個人因素與國際地理知識之間的關係。本研究以便利抽樣的方式選取台灣北部、中部、南部的三所普通高中的學生為研究樣本發放紙本問卷,期間為1個月,共發放402份紙本問卷,回收的有效問卷數為364份,問卷回收率為90.5%。資料以描述性分析、差異分析、相關分析和迴歸分析進行變數之間的關係探討。研究結果顯示:經過差異分析,我們得知文化智商、國際地理知識並不會因性別不同而有差異;同時也得知,國際地理知識會因個人文化智商分數的高低而有不同。而經過相關分析可以得出,國際地理知識與自評英文能力、人格開放性、文化智商、日常習慣呈正相關。最後,經由迴歸分析後可以了解到文化智商對國際地理知識有正向影響,同時人格開放性對國際地理知識也具有正向關係。最後,本文提供研究討論、結論、與研究限制以供讀者參考。

利用線蟲模式研究飲食對於神經老化的影響及其分子機制

飲食是否影響老化一直是個有趣的議題。秀麗隱桿線蟲有透明體腔、月餘的生命週期,為研究老化的極佳模式生物,和E. coli OP50相比,Comamonas DA1877會加速線蟲生長並縮短壽命。本研究旨在探討DA1877飲食是否造成線蟲早衰及其分子機制。實驗中先確認DA1877加速線蟲生長,並發現食用此飲食的線蟲具有較嚴重的頭部損害且擺尾速度減緩的老化特徵,說明此飲食使線蟲早衰。也採用Pmec-7::mRFP線蟲比較不同飲食下感覺神經老化狀況,更使用aldicarb藥物探討線蟲的癱瘓速率,發現DA1877不僅使線蟲早衰也改變感覺神經型態與運動神經功能。為了探討此現象的分子機制,採用與氧化壓力相關的核心轉錄因子daf-16 基轉種Pdaf-16:DAF-16a/b::GFP比較不同飲食下細胞核螢光顯現量,得知 DA1877使DAF-16入核表現,推測此飲食對線蟲而言為氧化壓力。已知OP50和DA1877在S-adenosyl methionine (SAM)含量上有顯著差異,未來希望以缺乏SAM合成酶之sams-1突變種進行實驗,了解DA1877使線蟲早衰的路徑。