人類脂肪幹細胞培養於電漿處理幾丁聚醣薄膜之初步研究
人類的脂肪幹細胞,取得容易,且來源不涉及倫理問題,是一種理想的城體細胞來源,目前已發現其可分化成骨細胞,軟骨細胞,脂肪細胞,等等,因此深具未來發展的潛力,將可應用於組織工程細胞治療上,而Chitosan(幾丁聚醣)則是一種具生物相容性,生物分解性且無生物毒性的材料,在生醫材料的領域一具相當發展性,在這個實驗中,我們將chitosan薄膜經過氬氣電漿處哩,並測量其與水的接觸角.發現電漿處理時間越長,接觸角越小,表示電漿處理有助於增加chotosan薄膜表面的親水性.細胞在經過電漿處理的薄膜上,其黏附情形一隨處理時間增長,黏著數目越多,且形態愈扁平,表示電漿處理過的chotosan表面有助於細胞的吸附.;Human Processed Lipoaspirate cells (hPLA) can be subtracted easily from lipoaspirate and the source of the cells does not violate the moral and ethic standard. Therefore, it is an ideal source of somatic stem cells. Recently, researches show that the hPLA cells have the ability to differentiate into osteoblast cells, chondrocytes cells, adipose cells, and skeletal muscle cells. In the future, they have great potential in tissue engineering or cell therapy. Chitosan is a biocompatible, biodegradable, and non-toxic material. It is also an advanced material to be used in the biomaterial field. In this study, we treated chitosan film with argon plasma and measured its contact angle with water. The contact angles decreased as the duration of plasma treatment on chitosan increased, indicating that plasma treatment has a positive influence on increasing the hydrophilicity of chitosan film surface. Moreover, the numbers of hPLA cells adhering to chitosan films increased and their morphology became flatter when the durations of plasma treatment on chitosan films prolonged. Hence, the result showed that plasma treatment on chitosan films also promoted the adhesion of hPLA cells on chitosan film surfaces.
消波一線牽-花蓮港港池共振
本實驗利用喇叭、鍍鉑黑電極、波型產生器、鎖相放大器、及sciworkshope750 介面卡組合一套系統,此系統可使偵測精密度大幅提升,使得花蓮港模型尺度變小(120×60×4cm),因此可節省實驗的成本與時間。本實驗希望藉由模型的建立,感測系統的整合,傅立葉分析,使研究者可在各種模型條件下,獲得水波傳遞的相關物理量。鑒於花蓮港喇叭狀港池經實驗後證明容易造成港內共振,且不同的入射波頻率不同,所造成的共振現象及共振位置相當複?。本實驗為縮小模型,但卻可測出的共振頻率、衰退時間及振幅且非常明顯(精密度達3.0×10-4cm/mV)。首要課題是要在港內消除反射的波,在模型實驗中,測量有無加消波線及加不同材質 的線時波衰退時間的長短,得到衰退時間牽線消波者比實驗組減少為0.2-0.6 之間;振幅減少為0.03-0.89 之間,且越鬆散、分支越多、孔隙越大的物質消波狀況越佳,所以毛線效果最佳,棉線次之而尼龍繩最差。 This study utilizes the loudspeaker, the platinum-plated electrode, Sweep Function Generators, DSP Lock-in Amplifier, and the sciworkshope750 interface to construct our system. The system will enhance the great precision of detection, which diminishes the Hualien Harbor model yardstick to 120*60*4(cm). Thus we can save the cost and time of the experiments. We combine the setting-up of the model, the integration of the sensors and the Fast Fourier Transform (FFT) system so that the researchers can obtain the physics-related data when the water transmits ripples in various kinds of conditions. Seeing that the bell-shaped Hualien Harbor is apt to cause resonance in its basin, which is confirmed by our experiments later, our primary goal is to find a way to diminish the waves reflected in the harbor. With the diverse frequencies of incident waves, resultant resonance phenomena and positions are rather complicated. Our experiments are based on a miniature model, but the resonance frequency, the time of wave declining and the wave amplitude are easily measured, with the precision up to 3.0×10-4cm/mV. In another experiment, we add strings made of different materials that can subdue waves. Compared with the records without strings, the strings can reduce the time of wave declining by 0.2-0.6 and can reduce the amplitude by 0.03- 0.89. We have come to the conclusion that strings made of looser, more-branching and bigger-interstice material perform better in diminishing waves. Hence, the knitting wool has the best results, followed by the cotton threads and then the nylon ropes.
曲率的奧秘
我們研究的主題是曲率,且以高中所學的函數為主。雖然大學已有曲率公式,但我們將其表示成高中生較易了解的型式,並且以f(x) 的方式呈現。我們在函數曲線上取不共線三點,構成一個三角形,並求出此三角形的外接圓半徑。再將所取三點逼近,所求之半徑即為特定點的密切圓,也就是曲率半徑。而此曲率半徑的倒數,就是所求的曲率,同時我們將公式帶入高中各常見函數,以導出函數上各點曲率。;Our study is about curvature, especially about the fuctions we learn in senior high school. In university, there is a certain formula for curvature, but we hope to change it into a form that can be easily accepted by senior high school students, and express the formula with f(x), the symbol of functions. We pick three incollinear points from the curve of a function, making the three points into a triangle, and figure out the circumradius of this triangle. Then, we approximate the three points to one of them, and the circumradius will also be the radius of the osculating circle of the point. We define the radius as radius of curvature. The reciprocal of the radius of curvature will be the curvature. Then, we use the formula to figure out the curvature of the functions we learn in senior high school.