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臺灣

吸菸、喝酒與嚼檳榔之基因毒性研究

流行病學資料顯示,近年來口腔癌發生率明顯增加。本研究之目的是探討口腔黏膜細胞微核發生頻率及彗星分析法之基因傷害指標是否與菸、酒、檳榔暴露有關,以及是否可以看到菸、酒、檳榔之交互作用。 研究對象為桃園地區山地鄉之民眾,利用問卷收集個人之菸、酒、檳榔及干擾因子之暴露資料,再採集樣本之口腔黏膜細胞及血液樣本,進行基因傷害評估。 結果顯示,口腔黏膜之微核發生率與檳榔之暴露相關性最大,可以解釋12.6%;然而,彗星尾部動量和尾部長度則以香菸暴露解釋力最大;同時,檳榔與香菸的基因傷害交互作用似乎存在。因此我們認為,口腔黏膜細胞之微核發生頻率對於檳榔暴露者應是不錯的基因傷害指標,而香菸暴露則以彗星分析法之指標較為敏感。 ;Epidemiological studies showed that the incidence of oral cancer increased significantly in recent years. The purpose of this study is to evaluate the genotoxicity of cigarette smoke, alcohol and betel quid use as well as the. interaction effect between these three risk factors. The study subjects were selected from residents lived in a mountain area of Tao-Yuan county. We used questionnaire as a tool of exposure measurement. The exfoliated oral buccal cells and blood samples were collected for the assay of micronucleus frequency (MNF) and comet assay. We find that there are higher MNF in smokers, alcohol drinkers, and betel quid users compared with nonusers. However, a significant difference can not be found because of small sample size. The relationship between MNF and three risk factors was strongest in betel quid use, however, for smokers the parameters in comet assay were more sensitive than MNF. There is a tendency of additive effect between cigarette, alcohol and betel quid although without statistical significance. Advanced statistical analysis is suggested to find stronger evidence in the genotoxicity biomarker.

奈米防蝕專家-微乳液法製備聚苯胺奈米粒子及其在防蝕應用研究

導電高分子在各面之應用非常廣泛,其中聚苯胺因價格便宜,製作簡便,使\r 其應用潛力更為突出。聚苯胺在鐵系及非鐵系金屬之防蝕能力已被證實,但由於\r 聚苯胺與金屬之附著力不良使其應用受到限制。奈米粒子所具有的表面效應、小\r 尺寸效應及宏觀量子隧道效應,使得奈米微粒材料之應用蓬勃發展。但在高分子\r 奈米微粒之製備仍屬有限。本文以微乳液法製備聚苯胺奈米粒子,以提高聚苯胺\r 與金屬間之附著力,使其防蝕能力充分發揮。國外雖已有廠家製作聚苯胺防蝕塗\r 料,但屬於商業機密無從得知其製備方法。本文所研發的微乳液法則是國內首\r 創!\r The development and application of the conducting polymer polyaniline is\r getting prosperous and popular. The capability of polyaniline in corrosion protection\r has been proved. But due to the adhesion of polyaniline on the metal is poor, the\r applications are restricted. By the way, the nanoparticles have the special effects such\r as the surface effect, the small size effect and the macro-quantum channeling effect\r make its applications are prosperous.\r In this paper, the authors utilized the microemulsion method to produce the\r polyaniline, to modify the adhesion of polyaniline on the metal in order to improve\r the effect of the corrosion protection of polyaniline in ferrous metal. The experimental\r results show that the nano-polyaniline has good adhesion on metal. The metal coated\r a layer of nano-polyaniline has the great ability of anticorrosion under different\r corrosion situations after weeks. The nano-polyaniline produced by the\r microemulsion method add the recipes invented by the authors has great potential to\r use in scale-up production in industry.

旋風手裡劍之瓶蓋棒球大解密

我們改裝了JSPB氣槍,藉以產生穩定的推進力,並結合3D列印製作不同的槍頭,加上砂紙的摩擦來模擬出手時產生的旋轉,利用這個發射器,進行不同的實驗。 我們使用了flash、tracker、MPC、excel等軟體,交叉比對實驗結果,並成功利用現有的器材對同時高速移動又高速旋轉的瓶蓋進行了轉速分析,克服沒有專業高速攝影機的困擾。 分析後,我們發現瓶蓋的凹面擺法是產生浮力的主因,可用以對抗重力,延遲下沉,而摩擦產生的旋轉會造成瓶蓋在行進中改變球路,利用不同長度的槍管,就能產生不同程度的旋轉,可有不同程度的球路變化,結合這幾項元素,我們成功實現多種球路,可提供有興趣的人練習打擊,希望在瓶蓋棒球運動的發展上做出貢獻,說不定會掀起風潮。

模擬聲波干涉

在高中光學裡,介紹了許多有關光波之特性,而聲波與光波皆具有波動性,因此聲波應具有如干涉、反射、聚焦等特性,但在物理課本上並未詳加敘述,所以我們開始了本項的研究,希望可以籍由改變聲源及邊界的各項條件,而探討其發生之現象。在本研究中,我們利用聲波之基本原理在電腦上進行聲場的模擬並加以改變其變因(頻率、相位、聲源數、聲源間距、強度、邊界反射),進而明瞭聲場之各項特性及應用與控制方式。經電腦模擬聲場圖中,我們觀察到,兩聲源干涉所形成之圖形為多組雙曲線所組成,近似於光學之雙狹縫干涉,增加聲音頻率與聲源間距離皆可使腹(節)線數目增加。如同現實世界中所知的,隨著頻率的增加,將會具有指向性的產生並且在聲源數目越多時越明顯,但發現頻率增加至一定值之後,指向性反而會降低而形成冠狀面。在延遲了多點聲源間相位之後,聲場分佈有偏轉之現象,利用相位延遲的方法,在多聲源中,將兩旁之聲音偏向中央將可造成聲音的聚焦。在兩聲源干涉中,調整其中一聲源之強度,將可完全消除兩音源連線間一點之聲音,可適當的應用在工業上消除噪音。聲場分佈在具有邊界的環境下,我們試著找出聲源位置及邊界條件對聲場分佈的影響與關係以模擬室內聲場,但在簡化的數學模式下,即無法有我們所希望之最均勻聲場分佈產生。最後我們將實驗中的結果與文獻上的實驗數據加以比較,以探討其誤差。 The optical course in senior high school , which introduced many characteristics of optical wave. However, both of sound and light have the characters of wave; therefore, sound wave should have the characteristics, such as interference, reflection and focalizing. Nevertheless, there are not many details of sound wave in the section of acoustic on our textbook. So we began this research, and discuss the different phenomena by changing many kinds of variables. In our research, we simulated the sound field on the computer, based on sound wave’s principle, furthermore we change many variables, which like frequency, phase, source number, distance, intensity and reflection. It helps us understand the characteristics of\r sound, how to control sound and how to apply these findings. According to the result of computer simulation, we discovered that the graph of two acoustic source’s interference comprised by many pairs of hyperbola, just like optical double slit interference. As the frequency or the sound source distance increased, acoustic direction became more and more obvious. But when the frequency was high enough to over the extreme, instead increasing, the acoustic direction would lower down like a crown. After we make phase differences on one of the two sound sources, sound field generated\r deviation. So if we use this method in multiple sound source, and delay the middle source, the sound field might be converged. In such two-sound-source interference pattern, when we control the intensity of one, a certainly point on the line of the two sources disappeared When the sound field enclose by borderline, the standing wave appear, and we discovered many funny phenomena. We put large amount of source in a narrow slit, the phenomenon of diffraction appeared. Finally, we discussed the discrepancies between interference pattern previously done by others experiments and the simulated one conducted by us.

環境標籤---地衣與環境污染的探討

隨著工商業發展,環境污染日益嚴重,對多數生長在這塊土地上的人,無疑造成了非常嚴重的影響。但若想要監控目前環境中的空氣品質,則必須具備專業的訓練,及昂貴的實驗設備,對一般民眾而言,根本就做不到。\r 藉由指標植物對所生長環境的高度敏感性,可以發展出一套純天然且免費的環境污染偵測器,不但方便、免攜帶、無須高級儀器協助、更不需要專業的分析技術。為此,我們以對二氧化硫等空氣污染物極為敏感的地衣作為指標植物,對其進行生態與環境污染關係的一系列觀察,並設計相關的實驗,找出環境污染物對地衣的實際影響,使其能夠實際的應用於日常生活,並可加以推廣,讓人人都可以利用地衣來了解自己所處的環境是否遭受污染,為自己家園的環境優劣把關。\r \r 文摘要 :\r With the development of industry and business , environmental pollutions become more and more serious . Undoubtedly , those pollutions have a great effect on us.\r However , by the means of the indicator plant which is highly sensitive to its environment , we can develop a set of natural and free environmental pollution detectors . In this project , we use lichenes,which are very sensitive to air pollution , to do a series of observations and to find out the influence the pollutants have on lichenes . If we can apply this to our regular lives , everyone can use lichenes to see if their environment is polluted or not .

智慧型控制界面系統

對數位、光電的初步了解是在小三的科展,當時我是利用一個大型的凸透\r 聚光鏡,匯聚太陽光照射在不同的金屬導線之一端,而另一端接在冰冷的地\r 板上,用不同的金屬材料來做比較。利用微電流計測得不同的材料有不同大\r 小的電流,而且有不同的熱量。到國中的電學時了解到電阻熱功率的關係,\r P=IV 才知道,除非有『超傳導系統』否則任何物體必有電阻。進入國中時,\r 在電工課程中了解了數位的控制,產生了這方面的興趣。\r

假如我是正常的?!—再探渦流脫離是否可能為聖嬰發生動力

聖嬰現象為全球的共同話題之一,由於其發生會影響全球性氣候的改變,使原本乾旱處下起傾盆大雨,原本潮濕氣候的地區成了乾旱地區,導致生命、財產嚴重性損失,這也是引發本研究想站在不同科學領域角度來探就聖嬰現象的發生的原因。目前科學家大都以大氣觀點來說明聖嬰的成因:太平洋上因風向改變引起海水流向或動能變化,導致太平洋赤道地區及南美洲西側海面溫度的改變,而觸發聖嬰的發生。由於海水的熱容量比空氣大,因此我們想以海洋觀點來說明另一種可能觸動聖嬰發生的動力,在生活經驗裡,我們發現水流通過障礙物會在後方形成渦流,因此,當南極繞極環流通過德瑞克通道受到南美洲南端阻礙時,有可能在南美洲形成週期性渦流生成及脫離的現象。「這樣的渦流是否存在?」、「渦流脫離後在南美洲左右岸海面溫度、高度是否發生變化?」、「此動力是否進而觸發聖嬰現象?」,是本研究期待以新思惟解釋引發這個全球現象的另一可能動力。

魚記猶存-觀賞用斑馬魚空間記憶的探討

本研究旨在探討環境對觀賞用斑馬魚(Danio rerio)空間記憶形成的影響。前人研究建構在斑馬魚空間記憶的腦區,或聲音、顏色等刺激對空間記憶的影響;本研究則證實了斑馬魚的記憶學習與環境具關聯性。本研究的原理是利用斑馬魚偏好深水區的天擇特性,加上改良自T字型迷宮的十字型迷宮,觀察訓練兩天後,斑馬魚進入深水區的耗時減少量,以推估空間記憶形成。從迷宮水體溫度及成分兩個向度進行探討,發現:低溫環境有助斑馬魚形成空間記憶。咖啡、酒精及茶有助形成空間記憶;人參、黃耆及菸草則無助於形成空間記憶。此外,本研究更運用自製的制約訓練器,發現空間記憶學習的最佳環境也能促進斑馬魚的制約行為學習,或許能進一步推廣到更多類別的長期記憶。

「混」是「膜」王─探討海藻酸鈉及澱粉混和薄膜的特性

本研究旨在探討海藻酸鈉、澱粉及兩者混和成膜的特性,並討論上述薄膜添加氯化鈣進行交聯後對薄膜特性的影響及應用,希望將薄膜進一步應用在生活中。研究發現支鏈澱粉含量多所組成的薄膜,黏性高、易破、厚度最薄;添加澱粉量愈多的薄膜愈硬脆,若提高海藻酸鈉濃度可使膜較為平整柔軟;與氯化鈣交聯成膜則會使薄膜厚度增加、面積縮小;添加澱粉的海藻酸鈣薄膜吸水率提高,3%糯米粉乾粉混合膜的吸水率達99%最高,薄膜乾燥前期的含水率下降速率較快,將此薄膜重複吸水,第一次吸水量最高,之後的吸水量減低且往復吸水、乾燥的速率提高,將薄膜添加水晶寶寶可使吸水量增加製成吸水墊,以期取代目前生鮮食材用的PE淋膜不織布吸水紙,將能更具環保價值。

擺動知覺曉,觸角知多少!--光線與震動影響美洲蜚蠊觸角擺動模式之研究

During the biology classes from junior to senior high, we have learned many interesting instance of different animal behavior. Most people paid more attention on the Vertebrates as their experimental subjects. The other species around us, although with simple body structures, may behave rather complicated and versatile reactions. In particular, one of the most common insects with simple body structure in our neighborhood is the American cockroach (Periplaneta americana). The aim of this study is to investigate the different swing motion modes of antennae of American cockroach by computer-aided Imaging Analysis. The parameters of each swing motion mode were calculated in order to analyze how light (including light stimulation and light adaptation) and vibration may affect the antennae behavior of American cockroach. It was found that the antennae swing motion modes were significantly different under different types of stimulus. If two types of stimulus occurred at the same time, the reactions of antennae motion may become conformable. In conclusion, antennae behavior has shown to significantly affect the survivability and environmental adaptation of American cockroach. Not only the antennae are considered as the sensitive receivers; but also they are the important transmitters to reflect physiological status and environmental condition.從國中到高中的生物課堂上,我們學到許多有趣的動物行為例子,但前人多以脊椎動物作為研究對象,而我們身旁的許多生物,身體構造雖較為簡單,但行為表現卻豐富多樣,尤其是常見的美洲蟑螂(Periplaneta americana),可說是最親近我們的昆蟲之一。本研究以攝影紀錄的方式,透過電腦進行影像分析,記錄不同刺激下蟑螂的觸角擺動模式,並計算出各項觸角運動的參數,以瞭解光線(照光刺激或照光適應)與震動刺激對其觸角行為的影響。我們發現在不同因子的刺激下,觸角擺動的模式具有差異,若兩種刺激同時發生,蟑螂觸角的行為亦具有整合性的反應,證明蟑螂觸角的行為模式,對其生存與適應具有重要意義。這也代表觸角除了為敏感的受器,亦為能反映出生理與環境狀態的重要動器。