臺灣

人類粒線體蘋果酸.可利用NAD+或NADP+為輔.，幫助腫瘤細胞獲得能量，但一般生理條件較傾向以NAD+為輔.。本研究將K346 修改成偏好NADP+之粒線體蘋果酸.家族中具有高度保留性的絲胺酸、及不具極性之丙胺酸，探討為何此酵素較偏好以NAD+為輔.。天然及突變株酵素的催化常數 (kcat)、基質Km 值、及抑制常數 (Ki) 測定結果顯示K346 之點突變不會影響基質Km 值，但K346S 之kcat明顯上升，繼而改變此酵素對NADP+之選擇性。本研究對於人類粒線體蘋果酸.催化機制的了解，有助設計具專一性的活性抑制劑，未來可應用於抑制腫瘤細胞能量來源，進而抑制腫瘤細胞生長。;Human mitochondrial NAD(P)+-dependent malic enzyme can help tumor cells gain energy, using either NAD+ or NADP+ as the cofactor, but prefers NAD+ as the coenzyme. By mutating the K346 to Ser, conserved in NADP+-dependent ME and to Ala with non-polar, we explore why human mitochondrial NAD(P)+-dependent malic enzyme prefers NAD+ as the coenzyme. We measured the proteins kcat, Km and the Ki values. The experiments showed that mutantions don’t affect the Km values, but K346S increased in the kcatvalue, transferring the coenzyme specificity to NADP+. If we develop deeper understanding of the human mitochondrial NAD(P)+-dependent malic enzyme, we can design a specific drag to inactivate the enzyme activity, and inhibit tumor cell growth.

本研究自2005 年12 月開始，以臺北市內湖區大湖自公園死亡的漂浮琵琶鼠魚(Many-rayed Sailfin Sucker Catfish )為主要研究對象，探討外來物種與放生行為對大湖生態系的影響。 研究期間共觀察到死亡的琵琶鼠魚隻數計有1085 隻，可記錄到個體形質資料的隻數為910 隻，背鰭棘數則有11 棘、12 棘、13 棘與14 棘四種，分析四種不同棘數的琵琶鼠魚後發現：在體長、頭寬及吻到背鰭長度均無差異(p>0.05)，因此判斷所記錄的個體應是棘甲鯰科(Loricariidae) Pterygoplichthys 屬中的同一種魚種。由檢視魚體並分析記錄數量與環境因子相關性後推測：琵琶鼠魚死亡主因是劇烈起伏的溫度差異，其次是人為因素的刻意傷害。 所記錄到大湖池塘水棲動物有：環節動物、軟體動物、節肢動物、魚類、兩棲動物及爬行動物等六大類共34 種，其中外來物種有16 種，本地入侵物種有3 種，而這些外來動物進入大湖的主要管道應是由個人的棄養或放生行為所造成。琵琶鼠魚因繁殖能力強、具攻擊性、吞食其他魚種卵塊、可適應高污染水體及垂釣客對魚種的篩選等因素，而成為最具生存競爭能力的優勢魚種。不但造成大湖池塘呈現嚴重魚種單一化，更可由靜止水域大量擴散至流動水域。未來如何將政府相關部門、學術研究單位與民間社團等力量結合，共同防範大湖琵琶鼠魚的持續蔓延、宣導民眾正確的放生觀念、積極改善大湖水體污染狀況，並訂定完整之外來物種移除計畫，以期恢復大湖池塘物種歧異度，都將是刻不容緩的重要生態課題。;This research began in December of 2005. Focusing on dead floating Many-rayed Sailfin Sucker Catfish in Dahu Park, Nei-hu Distrct, Taipei city. We discuss the impacts of Alien and of behaviors of the release of captured animals on Dahu Park’s ecosystem. During observation period, there were totally 1085 dead Many-rayed Sailfin Sucker Catfish, of which 910 bodies were found. The number of the thorn bushes on dorsal fins includes 11, 12, 13,and 14. After analyzing four kinds of different thorn bushes, we find that no differences exist in Total length, Head width, Predorsal length (p>0.05). We hence conclude that the recorded object should be species with identical with Loricariidae Pterygoplichthys. With the inspection of the fish’s body and the analysis of the relevance of the recorded quantity and the environmental factor, we infer that the main reason of the Many-rayed Sailfin Sucker Catfish’s death is violent temperature differences and the second is attributed to human’s intentional abuse. The aquatic animals of Dahu Park amount to 34 kinds and six classes such as Annelida, Mollusca, Arthropoda, Pisces, Amphobian, and Reptilia. Among them, 16 kinds that are Alien and 3 kinds are Native Invasive Species. Intentional abandon and release behavior channel of these Alien to Dahu Park. Many-rayed Sailfin Sucker Catfish are highly productive, aggressive, and adaptable to highly polluted water. Besides, they eat ovums from other fishes. Moreover, they are not the fisher’s preference and hence often thrown back into pond once hooked . Thus, they become the most competitive survival fish species in Dahu Park. The high competitive ability of Many-rayed Sailfin Sucker Catfish causes Dahu Park to present a serious unification of fish species. This serious unification of fish species could also be spread from static water areas of Dahu Park to flowing water areas. According to our research, certain urgent ecological issues in Dahu Park are to take precautions against the spread of Many-rayed Sailfin Sucker Catfish, to promote the correct idea of releasing captured animals, to improve Dahu Park’s water pollution, and to stipulate a complete plan about eliminating Alien. Our research suggest that government’s relevant departments, academic research units, and folk corporations should be cooperated to achieve the above four goals. Once the four goals are achieved, we believe that the fish species of Dahu Park will be full of varieties again.

在模式植物──阿拉伯芥（Arabidopsis thaliana）中，AtYAK1（Arabidopsis thaliana Yak1-related protein kinase）是目前發現唯一屬於DYRK（Dual specificity Yak1-Related protein Kinase）的蛋白激?。雖然之前研究已證明，不同物種之DYRKs 和細胞的生長與發育過程有關。然而，其在植物中的生理功能卻尚未被明確地研究報導過。在先前的研究中，為瞭解AtYAK1 在阿拉伯芥內作用之位置，前人選取AtYAK1 基因ATG 上游約2.5 kb 的序列（Upstream Element, 2.5KUSE）建構至一含有GUS（β-glucuronidase）報告基因的質體中，並轉形至阿拉伯芥，進行GUS 組織染色分析。但在初步結果中，並沒有在轉殖株觀察到明顯的GUS 表現。進一步分析，我們發現在2.5KUSE 序列末端約0.5 kb 的5’非轉譯區（5’untranslated region, 5’UTR）中，有四組開放讀序框（Upstream Open Reading Frame, uORF）。有趣的是，許多研究也顯示，uORFs 會影響轉譯過程中的再起始（re-initiation）作用而調控該基因的表現。另一方面，前人亦透過構築好的2KUSE 轉殖株（即不含有5’UTR）進行上述GUS 實驗。結果發現，此2KUSE 轉殖株的GUS 表現非常顯著。本實驗即要瞭解AtYAK1 的uORFs 是否也會影響其蛋白質的合成。首先，我們以點突變的方式將四組uORFs 中之ATG 換成TTG，目的為構築不含有uORFs 之5’UTR（mutated uORFs, ΔuORFs）。在進行原生質體短暫表現分析法（protoplast transient assay）及GUS 組織染色分析後，將結果與含有uORFs 的結果作比較：當缺乏uORFs 後，其3’端報告基因的表現量確實比原來顯著。綜合以上，我們認為此uORFs 對於AtYAK1 蛋白質之表現佔有相當重要的影響地位。最後，我們對5’非轉譯區是否存在開放讀序框進行阿拉伯芥全基因組分析，相關結果亦於本研究報告中分析討論。AtYAK1（Arabidopsis thaliana Yak1-related protein kinase）is the first DYRK（Dual specificity Yak1-Related protein Kinase ） family member identified in the model plant ─ Arabidopsis thaliana and exists as one copy gene in Arabidopsis. Previous studies showed that many eukaryotic DYRKs are involved in regulating the growth and development of cells. However, the study of AtYAK1 in Arabidopsis is lacking to date. In order to understand where AtYAK1 expresses and functions in plants, a 2.5 kb fragment which is located upstream from the major ATG of AtYAK1（termed Upstream Element, 2.5KUSE）was previously constructed to drive the expression of a reporter gene, GUS（β-glucuronidase）, in transgenic Arabidopsis. Much to our surprise, no GUS expression signal could be detected in such transgenic plants. When further analyses were performed, we found that there are four upstream open reading frames （uORFs） in the 5’untranslated region ( 5’UTR ) within the 2.5KUSE. Many studies indicating that the uORFs can regulate the translation of downstream ORF encoding the major gene product through the procedure of translation re-initiation. This action represents a mode of translational regulation for gene expression. Indeed, GUS activity could be readily detected in transgenic plants expression 2KUSE::GUS, a construct lacking the 5’UTR of AtYAK1. In this study, I have tried to elucidate whether the uORFs of AtYAK1 can regulate the translation of the downstream major ORF. First, in order to construct a 5’UTR fragment of which uORFs have been mutated（ΔuORFs）, we apply site-directed mutagenesis to substitute ATG with TTG for the four uORFs and examine the expression of GUS driven by this mutated 2.5KUSE. After analyzing the results in both Arabidopsis protoplast transient assay and transgenic Arabidopsis, stronger expression of reporter genes in both systems were observed when the four uORFs were mutated. We have also confirmed that, in transient expression system, the increase of reporter gene activity was not due to the excess accumulation of the corresponding mRNAs. Rather, it is the four uORFs which play an important role in negatively regulating the translation of AtYAK1, possibly via inhibiting the translation re-initiation of major ORF. A genome-wide examination of uORFs in all Arabidopsis genes was also performed to assess the possible contribution of uORF in regulating gene expression.

本研究利用波以耳定律為基礎，以理論計算推導的方式，模擬水氣壓計的操作模式，並 探討其校準模式與誤差來源。 以此設計出的水氣壓計有以下兩個特色： 1. 大氣壓力的變化與水氣壓計液面的變化為線性關係。 2. 可以得到比水銀氣壓計更佳的靈敏度。 而在水氣壓計的校準上分別探討了標準校準、壓力差校準、溫度校準與重力密度校準。 利用標準校準可以得到水氣壓計液面變化與大氣壓力變化的線性關係式。而溫度校準與密度 校準在儀器操作環境為定溫下則可併入標準校準。 This study based on Boyle's law imitates the operation model of water barometer and discusses its calibration and sources of error. The designed water barometer has two features: 1. The relationship between atmospheric pressure and the height changes of the surface of the water barometer is linear. 2. More sensitive than that of a mercury barometer. We also discuss standard calibration, pressure calibration, thermo calibration and gravidensity calibration. The linearity between atmospheric pressure and the height changes of the surface of the water barometer is known by standard calibration; thermo calibration and gravidensity calibration can be merged into standard calibration under constant temperature surroundings.

本研究目的在探索不同大小之氣泡在不同黏滯性液體中運動情形。實驗結果發現大氣泡向上運動的速度較大，其下方會漸漸向內凹。並且觀察到氣泡間結合時的相互作用：氣泡在相同黏滯性膠水中上升時，若下方氣泡體積較大，其較快的速率會使距離縮短。此時小氣泡的下半向內凹，大氣泡的下半則向外呈現流線型尖端並且在接近小氣泡時速率增加，最後與小氣泡結合。若上方氣泡體積很小，與下方大氣泡的距離縮短至相互貼合，小氣泡會先停留在大氣泡的上半表面，再沿大氣泡表面下滑至大氣泡的下半才與大氣泡結合。This research traces the motions of bubble with different volume in viscid liquid. The experimental results show that the bigger bubble rises at faster speed. The shape of the small bubble is round. As the volume of the bubble increases, it turns hamburger-like. And if the bubble is big enough, its underside would be concaved. In viscid liquid, the speed of the bubble is not smooth but waved. The smaller the bubble is, the more the variation in speed is. The interaction of two bubbles is also studied. There are two types of the combination of two bubbles. While the big one closes to the small one, it is accelerated. The underside of the small one becomes concave. And the big one becomes streamline shape. If the difference in volume between two bubbles is significant, the small one slides along the surface of the big one, and goes into the concave beneath it, then combines with it.

我們藉由電腦模擬來研究宇宙微波背景輻射中之Sunyaev-Zel’dovich 效應，以探討星系團及宇宙的一些根本性質。重要的發現有： 以上的結果，將可在短期的未來直接應用在許多期待中的觀測結果上，以揭開星團的總質量、質量密度、以及宇宙中的黑暗能量等神祕面紗。 We study the important properties of the galaxy clusters and our universe by using numerical simulations for the Sunyaev-Zel’dovich effect in the Cosmic Microwave Background. We found that: These results can be applied to the observations in the near future, in order to reveal the total mass of clusters, their mass density profile, and the dark energy of our universe.

大安水蓑衣（Hygrophila pogonocalyx）為局限分佈於臺中縣沿海溼地之稀有植物。大量境外復育栽殖後，造成近年來黑擬蛺蝶(Junonia iphita iphita)利用此種新寄主植物的比例增加。本實驗比較黑擬蛺蝶在原寄主植物臺灣馬藍(Strobilanthes formosanus)及新寄主植物大安水蓑衣上之生長環境遮蔽度、幼蟲生長發育、雌蝶產卵偏好的差異，探討大安水蓑衣復育對黑擬蛺蝶族群可能造成的影響。實驗結果發現利用大安水蓑衣之幼蟲生長發育較佳、羽化後成蟲體型較大、有效積溫常數較低。雌蝶產卵行為可能存在兩種偏好性，且子代雌蝶對寄主植物的偏好性與親代一致，不受幼蟲期取食植物影響。偏好新寄主植物之雌蝶其子代的生長發育，利用新寄主植物者顯著較利用原寄主植物者佳，顯示其對原寄主植物的適應顯著下降。本研究認為大安水蓑衣的復育結果，造成黑擬蛺蝶族群在可能共域的情況下，因為利用新的寄主植物可能已產生初步分化的現象。Hypgrophila pogonocalyx is a rare plant species which is distributed in wetlands along seashore of Taichung County. Recently, I found that the common butterfly Junonia iphita iphita recognized H. pogonocalyx as a new hostplant because of the restoration practices of H. pogonocalyx. We compared the differences of forest overstory coverage, larval growth performance and female oviposition preference between J. iphita iphita individuals exploiting the H. pogonocalyx and those utilizing the original hostplant Strobilanthes formosanus. Possible effects on J. iphita iphita by restoration of H. pogonocalyx are analyzed and discussed. It turns out that better growing performance and longer adult forewing length were found on the larvae feeding on H. pogonocalyx than those on the S. formosanus. Besides, the larvae feeding on H. pogonocalyx demonstrated lower constant value of effective accumulated temperature, suggesting that the new host may provide more energy to the larvae than the original host does. Two types of female oviposition preference seem to exist. No matter which hostplant the larvae fed on, the female adults still maintained the oviposition preference of the parental generation. Offspring of H. pogonocalyx-preferring female had better performance on this new hostplant. Our studies showed that the restoration of H. pogonocalyx might have caused primary differentiation of J. iphita iphita by using new hostplant in sympatry with the original hostplant.

本研究以高頻阻抗分析儀，針對自製的電容器，以不同比率的三氧化二鐵加二氧化矽混合後所製成的電容器，對頻率、電容直及正切損耗能量直從事研究。實驗結果發現，在低頻率直到60Hz以內，其 Cp及D值變化非常大，明顯的下降，60Hz到1000Hz Cp及D值變化很小。由參考資料中電容器中的損失因數是介電材料是否適合於絕緣材料用途之最基本的準則，一般均希望具有低介電率。即使在最小的空間內也希望有高電容值，如電視、收音機中在介電材料的高頻應用。是故我們所製作的電容器以二氧化矽添加三氧化二鐵後高頻值其Cp值、D值較小，是適合於高頻需求的應用上。We report on the study of high frequency dielectric spetra. On the capacitance, it is made by mnyself. At various mixed ratio value of Fe?O? pius Sio?. In order to understand the frequency capacitance value Cp and tangent loss energy analysis D. The results of our work provide a large of low frequency in o Hz TO 60 Hz in Cp and D. It is obviously descend a small various frequence is 60 Hz to 1000Hz. From reference, we know capacitance energy loss factor is a purpose of suitable an insulator rule. In dielectric constant. We hope a low dielectric value and high capacitance. Even it is a small distance. For example TV. Radio. Etc. it is used to high frequency. So we made a capacitance in difference of Fe?o? pius Sio?. To provide the Cp and D. the value is small. So it can be used in high frequence demand.

Earthquakes are results of rapid sliding of two crystal blocks around fault inducing seismic motions. Huge disasters caused by large earthquakes as the Ji-Ji Earthquakes occurred on 21* September 1999 show importance of earthquakes forecast. In our country and foreign countries , there appeared not a small number of reports suggesting there are clear precursors of ground water and electro- magnetic changes amount other numerous phenomena. Recently there are reports that point out the possibilities to investigate slight motion of ground water near active faults using electro- magnetic instruments. In order to investigate the problem more in detail a simple laboratory experiment is designed to study the electric field induced by the simulated water flow. The electric field changes are compared with at without water flow. It is shown that there appeared electric potential changes of an order larger than measurement limits water motion during earthquakes occurrence process, and that monitoring the flow of ground water near active faults is useful method in the earthquake prediction.近年來科學家從事地震研究，發現地震前斷層附近地下水流以及電磁場會發生變化。但或因位置不確定，或因變化量甚小而不易量測。本研究真對上述地震預警中兩項重要的關鍵 - 水及電，嘗試製作電極，並利用室內模擬地下水流動而野外斷層的電位量測，檢測斷層活動期間電場的變化。其結果顯示，當地下水因地殼受擠壓而流動時，可明顯觀察到電場的變化。這表示借由本實驗測量地震前發生的電位變化，可監測地震前版塊應力的改變，進而提供地震可能發生的資訊。

本研究以b04課程中的巴斯卡三角形為研究對象，將原先巴斯卡以「1」為首、「+」為運算符號的規律三角形，改為以「-1」及「ω 」為首、「×」為運算符號，分別就其產生的新三角形作探討，發現其中似乎隱藏著原先三角形所沒有的規律性。為了更瞭解這種規律，藉由電腦軟體繪出其圖形，圖形顯示出如碎形般的複製關係，不論放大或縮小，其中的遞迴關係並未改變，頗令人好奇，因此著手研究。研究過程中對於圖形的規律性採用先臆測、接著歸納、最後給予證明的方式呈現。得到以下的結論：一、分別以數列呈現新三角形圖形的規律性。二、分別將新三角形中每一列中的某數字（如-1、ω 或ω 2 ）的個數予以通式表之。三、分別推算出新三角形第n 列第j 行的數是「1」或「?1」及「1」或「ω 」或「ω 2 」。四、相同的模式，在特定的圖形範圍中，不斷重複出現。許多研究將巴斯卡三角形中的所有數，以某數為模的餘數紀錄下，去探討其餘數在新產生的巴斯卡三角形中的分布情形；而在碎形的研究中，大部份著重如何畫出碎形。本研究著重圖形其規律性的探討，提供上述研究不同角度的詮釋與探討。 This research subject is based on Pascal’ s triangle in senior high school curriculum. The regular triangle begins with「1」and use「+」as operation. Let 「1」 be replaced with「-1」and「ω 」, the operation sign「+」be changed into「×」. I do research on the new triangle and discover the seemingly hidden regularity which doesn’t exist in the original one. To understand more about this regularity, I draw figures through the computer. The figures show the relationship of reproduction as fractal. Whether the figure is enlarged or minimized, it’s surprising curious the recursive relationship doesn’t change, so we begin to work on research. In the process of the research, we make careful observations, assumptions and deductions about the regularity of the figure. Finally, we come to some conclusions by means of giving proofs:（1）Present the regularity of the new triangle figure with progression.（2） Present such numbers as「-1」, 「ω 」, 「ω 2 」 in each row of the new triangle with formulas separately. （3）Figure out the number in the row n and in the column j of the new triangle is「1」or「-1」,and「1」or「ω 」or「ω 2 」. （4）The same model appears again and again in the specific range of figure. Many researches record Pascal’s triangle modulo certain number to explore the distribution of remainders in the new triangle. In the research of fractal, how to draw fractal is mostly focused on. The exploration of this research emphasizes the regularity of figure, offering the interpretation and exploration of researches above from different angles.