臺灣

運用自製低頻噪音( The relations between vehicle average speed with low frequency engine exhaust noise(

我們藉由電腦模擬來研究宇宙微波背景輻射中之Sunyaev-Zel’dovich 效應，以探討星系團及宇宙的一些根本性質。重要的發現有： 以上的結果，將可在短期的未來直接應用在許多期待中的觀測結果上，以揭開星團的總質量、質量密度、以及宇宙中的黑暗能量等神祕面紗。 We study the important properties of the galaxy clusters and our universe by using numerical simulations for the Sunyaev-Zel’dovich effect in the Cosmic Microwave Background. We found that: These results can be applied to the observations in the near future, in order to reveal the total mass of clusters, their mass density profile, and the dark energy of our universe.

線蟲為植物發生病蟲害感染的病源之一，而台灣的松樹，目前正面臨著松材線蟲入侵的危機。從文獻的探討中，發現線蟲有其自然界的天敵 － 線蟲捕捉菌。本實驗著重在探討線蟲捕捉菌特殊的捕捉機制。當線蟲捕捉菌附近出現線蟲時，會展生誘引線蟲的物質，並設計了一步步的實驗，去探討此誘引物質的捕蟲效能及其成分。現在，已經發現此誘引物質為一揮發性氣體。往後將會設法增加其誘引氣體的產量，並使用氣相層析儀分析之。最後希望可以將此物質應用到微生物防治上，期望能解決台灣松樹被線蟲感染的問題。 In Taiwan, all of the pine trees have one common problem - nematodes, which causes diseases in plants. And this experiment focuses on the nematodes’ natural enemy - nematophagous fungi and its “peculiarly caused mechanism.” When nematodes appear near nematophagous fungi, the latter will produce some substance to tempt the former. To investigate this alluring substance, a series of experiments are done and systematic steps are taken. The first finding is that this substance is a volatility gas. Later in this research, measures will be taken to make “rematophagous fungi” produce more of this gas. And “gas chromatograph” will be used to analyze this gas in the future. Finally, the possibility of applying this substance to the defensive measure of microbiology will be discussed.

古籍上記載山藥益肺氣，養肺陰，且最近研究報告發現山藥含有珍貴的dioscorin，其為山藥貯存養分的重要蛋白質，有抑制胰蛋白水解?之活性。而塵?糞便、屍體中的消化蛋白?會破壞呼吸道上皮細胞緊密連接處引起過敏氣喘反應。我對此甚感興趣，進而利用山藥的萃取蛋白dioscorin、塵?粗萃取蛋白及呼吸道上皮癌細胞A549，藉細胞培養、免疫螢光染色、螢光顯微攝影來實驗山藥是否真能保護上皮細胞緊密連接處。再利用膠體電泳、西方墨點法中抗體的高專一性、二抗的高靈敏度來確知我之前的實驗。另外也用ELISA 來實驗dioscorin或塵?引起A549 發炎的情況是如何。由實驗得知，dioscorin 可抑制及預防塵?引起的過敏氣喘反應；而經由ELISA 實驗發現，dioscorin 對於塵?刺激A549分泌發炎物質Eotaxin 並沒有助長或是降低的效果。It was believed that Chinese yam have benefits in treating asthma. Recently, it was found that the valuable dioscorin can be isolated from Chinese yam. The major function of dioscorin is nutrition-storage, however, it also have activities in inhibiting trypsin-like protease. This inhibitory activities trigger my interests. Because the major allergens, mites, and their stool may destroy the tight junctions of airway epithelium cells through their trypsin-protease activities. I therefore carry out an in vitro study to identify whether dioscorin can be used in protecting epithelium from the attack of mites. My results showed that dioscorin can protect the tight junctions of A549 cells from the attack of mite crude extract protein. I believed that dioscorin can be a good candidate in pharmacology application. The genetic and proteomic information are my further focus. On the other hand, we also investigated the possible activity of dioscorin in inhibiting mites-induced inflammation through using A549 as a model and employing ELISA. We found dioscorin neither inhibited nor enhanced the eotaxin being secreted from A549 under the stimulation of mites.

In this study, we mainly explore the geometric construction in 3D. By conducting some problems about constructing circles, we define the PLC construction in 2D as constructing a circle, either passing through a given point (P), tangent to a given line (L) or tangent to a given circle (C). Besides, we aim to discuss the properties of the PLC construction and the relations between each other. We discover if we find a plane satisfying certain conditions in space, the properties in the PLC construction can apply to such a plane. Furthermore, we extend the properties in PLC to the PES construction in 3D, defined as constructing a sphere, either passing through a given point (P), tangent to a given plane (E) or tangent to a given sphere (S). Also we discuss the relations among them.這個研究主要在探討3D 的尺規實作。藉由歸納某些有關作圓的題目，我們定義2D 中的PLC作圖─作圓，過已知點（P）、切已知線（L）、切已知圓（C）。並探討PLC 作圖的性質及彼此的關聯性。而我們發現：在空間中只要找到滿足特定條件的平面，則2D 幾何作圖性質在該平面仍能沿用。此外，運用PLC 作圖性質，我們進一步推廣到空間中的PES 作圖─作球，過已知點（P）、切已知面（E）、切已知球（S），並探討各個類型間的關聯性。

本研究主要是以一連串的地震分析，來討論台灣地區地震能量的釋放型態。 首先是求出地震的活動參數，接著再繪製地震規模的年發生率，最後是推算台灣地區 地震能量釋放時前餘震與主震所占之比重。 在地震參數上，發現地震活動度較高的月份中，在該月都發生了芮氏規模六級以上的 大地震，因其餘震發生的頻率較小震頻繁，因此造成其a值較大。 在地震規模年發生率方面，我們繪製出圖表，而最後發現台灣地區的地震仍以小震多，另外大地震則有一定的週期。 在研究的最後，了解台灣地區的地震常數之後，本研究以ESRI公司的GIS軟體ArcView8.3版疊合各種圖層，分析人口密集區及水庫的地震危害度分析，提出地圖，對大台北地區，人口稠密的幾個行政區提出警訊；另外在水庫方面，本研究則對幾個在斷層線上的水庫提出警訊。 ;This research presents a series ofearthquake analyses to discuss earthquake’s energy release type. First, we evaluated earthquake’s constant and charted the percentage of earthquake measurement every year. Finally, count the after-shocks and the major earthquake measuring 6.0 and above on the ML happening in Taiwan area. Because the after-shock frequency is more than light earthquakes, as a result it causes the “a” enumeration is large. According to the percentage of earthquake measurement happening frequency every year, we illustrate a chart. Finally we find that in Taiwan area mirror earth-quakes account for a large percentage and major earthquakes happened regularly. When we realize the constant of earthquake, the study folds a variety of layers by using ArcView 8.3 edition of GIS (Geography Information System) of ESRI co., LTD, analyzing the density-populated areas and the dams for seismic hazard to export the map. We raise a warning for the greater Taipei where some districts are densely-populated, and we also warn the dams on the line of fault of large of damage.

PPO 是一種含銅的多酚氧化? (E. C. 1. 14. 18. 1)，主要是將酚類 (phenol) 氧化成二酚類(diphenol)，更近一步的變成quinones，後者是一種不溶於水的褐色聚合物，在植物中造成褐化最主要原因，然而在人類皮膚則產生黑色素 (melanin)。我們提出有一種揮發性的抑制劑存在動植物體內，此種抑制劑會抑制生物體內PPO 的活性。植物在收成後，揮發性的抑制劑逐漸消失，導致內生性的PPO 活性逐漸上升，因此植物便會產生深褐色的斑點。在本研究中，我們以玉蘭花作為例子，以生化酵素動力實驗、部分純化黑色素抑制劑來解釋揮發性抑制劑在植物體內的存在及其作用，更進一步對水果快速褐化提出一種新的理論。研究發現PPO 的褐化反應就像是人體內酪氨酸? (tyrosinase) 的催化反應，酪氨酸?可以使人體產生黑色素而累積在皮膚上形成黑斑，利用〝人工皮膚〞模擬揮發性黑色素抑制物的作用，證明，防止PPO 抑制劑之揮發可能在未來美白保養品工業裡扮演一極具潛力的角色。Polyphenol oxidase (PPO) or tyrosinase (E.C. 1.14.18.1) is an important and ubiquitous enzyme responsible for browning in plants and melanization in animals. PPO is a copper-containing enzyme that catalyzes the chain-oxidation from monophenol or polyphenols to o-diphenols and subsequent o-quinones. The resulting quinones are large wate-insoluble polymers with dark brown color. We proposed that volatile inhibitors are associated with the plant PPO and block the PPO activity in vivo. While post-harvesting the volatile inhibitors evaporate, the endogenous PPO is then activated and therefore instantly produces dark quinone pigment. In the present study using magnolia flowers as an example, we show the presence of a potent volatile inhibitor(s) for PPO in plant. The novel finding clarifies the mechanism involved in the browning phenomenon of post-harvesting for most fruits. Since the PPO is also know present in human as tyrosinase responsible for the formation of “darkening spots” on skin, the finding of evaporation of potent PPO inhibitor may be potentially used as a strategy in developing a novel cosmetic product.

在機械系統中，物件表面常產生磨損，但重要物件的磨損將會危及系統的操作及安全。本研究建立了表面磨損自動偵測警告系統，該系統包含監測系統、無線射頻資料讀取器、被動式無線射頻識別標籤(tag)、及電磁波隔離層等，並採”故障觸發”之方式運作。亦即在物件之表面下特定深度埋設有被動式無線射頻識別標籤，但該標籤外層覆有一層隔離層可隔絕該射頻資料讀取器之電磁波。 當物件表面尚未被磨損到特定深度前，讀取器之電波無法穿透電磁波隔離層而激發無線射頻識別標籤，因此無法讀到識別標籤之信息，系統將判定物件磨損狀況良好。等該物件過度磨損時，會把該電磁波隔離層磨除掉，使讀取器之電波可激發無線射頻識別標籤，而讀取到識別標籤內含之信息，系統因此可判別哪一個物件已有嚴重磨損之狀況，而自動提出警告信號。由於使用被動式識別標籤及故障觸發方法，本系統具有體積小、免電池、免維護、成本低、誤判率低等優點。測試結果以車輛輪胎的磨損為對象，證實本系統可以即時有效的偵測到車胎之過度磨損，保障行車之安全。

人類粒線體蘋果酸.可利用NAD+或NADP+為輔.，幫助腫瘤細胞獲得能量，但一般生理條件較傾向以NAD+為輔.。本研究將K346 修改成偏好NADP+之粒線體蘋果酸.家族中具有高度保留性的絲胺酸、及不具極性之丙胺酸，探討為何此酵素較偏好以NAD+為輔.。天然及突變株酵素的催化常數 (kcat)、基質Km 值、及抑制常數 (Ki) 測定結果顯示K346 之點突變不會影響基質Km 值，但K346S 之kcat明顯上升，繼而改變此酵素對NADP+之選擇性。本研究對於人類粒線體蘋果酸.催化機制的了解，有助設計具專一性的活性抑制劑，未來可應用於抑制腫瘤細胞能量來源，進而抑制腫瘤細胞生長。;Human mitochondrial NAD(P)+-dependent malic enzyme can help tumor cells gain energy, using either NAD+ or NADP+ as the cofactor, but prefers NAD+ as the coenzyme. By mutating the K346 to Ser, conserved in NADP+-dependent ME and to Ala with non-polar, we explore why human mitochondrial NAD(P)+-dependent malic enzyme prefers NAD+ as the coenzyme. We measured the proteins kcat, Km and the Ki values. The experiments showed that mutantions don’t affect the Km values, but K346S increased in the kcatvalue, transferring the coenzyme specificity to NADP+. If we develop deeper understanding of the human mitochondrial NAD(P)+-dependent malic enzyme, we can design a specific drag to inactivate the enzyme activity, and inhibit tumor cell growth.

In this research, we investigated the interactions between goldfish’ (Carassius auratus) sensory experiences (electric shock, high osmotic pressure and colors). We separated the sensory experiences into two parts. One of them, we used different kinds of environmental color-models to analyze goldfish’s environmental preference behavior and different kinds of stress experience(electric shock and high osmotic pressure) to build the animal model of environmental preference behavior. We found that goldfish prefer brown-colored environment (compared with white) and different stress has different effects on fish’s environmental preference behavior. It proves that fish has the ability to learn and memorize the stress experience and shows that there are complicated interactions among environmental stimulus, memories of stress and behaviors caused by different kinds of stress. Also, we investigated the interactions between spatial memory and color distinction. We measured the duration of goldfish getting through the color-marked barriers driven by regular electric shock. We found that goldfish spend less time to get through color-marked barriers than those unmarked. It shows that there are some interactions between spatial memory and color distinction. In conclusion, the research proves that different sensory experiences can affect goldfish’s behaviors.本實驗利用感官經驗(電擊、高滲透壓、色彩)，探討與朱文錦(Carassius auratus)行為調控的交互作用。我們將感官經驗分成兩模式：利用環境色彩特徵的差異，探討不同逆境刺激經驗(施予電擊或高滲透壓環境)，與魚類環境偏好行為的交互作用，建立朱文錦環境偏好行為之動物模式。我們發現朱文錦偏好土色環境(相對於白色環境)，而不同性質的逆境經驗，對魚類的環境偏好行為具有不同的影響，顯示魚類具有記憶逆境環境的學習能力，且證明「環境刺激—經驗(記憶)—行為表現」三者之間具複雜的交互作用。另外，我們亦探討空間記憶與色彩辨識的交互作用，利用規律的電擊刺激使朱文錦通過有色彩標示的關卡，以研究色彩差異與空間變化對朱文錦通過關卡能力的影響。我們發現色彩的標示可降低通過關卡的時間，且不同的色彩具不同程度的效果，顯示空間記憶與色彩辨識具交互作用。本實驗證明了不同的感官經驗或刺激，對朱文錦的行為調控具質與量的差異。