安非他命對小鼠中腦紋狀體之細胞凋亡相關蛋白質表現的影響
安非他命的濫用在台灣是非常嚴重的公眾健康及社會問題。安非他命會導致一連串的行\r 為異常,包括在中腦紋狀體內釋放多巴胺及阻止多巴胺回收來增加使用者的活動力。由於安\r 非他命會對腦細胞造成傷害,本研究的目的為探討低劑量、無立即毒性之安非他命(類似於人\r 類使用習慣)於短期內是否會對老鼠大腦紋狀體內的蛋白質表現有影響。因此利用西方點墨法\r 分析施打低劑量安非他命(2 到6 mg/kg)約一星期之後,C57BL6 小鼠的大腦紋狀體中一些重\r 要蛋白質[包括腺?酸受體A2A-R、第五亞型腺?酸環化?AC5、caspase-8、PARP、NF-κB\r 及血紅素加氧?-1(HO-1)]的表現是否有改變。實驗結果顯示,低劑量安非他命處理對大部分\r 蛋白質的表現並沒有明顯的差異,但在施打安非他命老鼠之大腦紋狀體中,HO-1 有些微但明\r 顯的增加,顯示安非他命可能對腦組織產生氧化性傷害。因此長期使用安非他命對中腦紋狀\r 體是否造成傷害是值得關心及繼續探討的課題。The wide spreading use of amphetamine (AMPH) in Taiwan has become a serious\r public health and social problem. AMPH evokes a series of behavior abnormality including\r enhanced locomotor behavior by releasing dopamine and inhibiting dopamine-uptake in the\r striatum. Since AMPH is known to cause brain damage, the purpose of this study is to\r investigate the expression of several important proteins in the mouse striatum after\r treatment with low and non-toxic dosages of AMPH for a short period (mimicking the\r common usage pattern of humans). C57BL6 mice were daily IP-injected with various doses\r of AMPH (0 to 6 mg/kg) for one week. Expression levels of adenosine receptor A2A-R,\r adenyl cyclase type 5,caspase-8, PARP, NF-κB and heme oxygenase-1 (HO-1) in the\r striatum were analyzed by Western blotting technique. Most proteins examined were not\r affected by the 1-week AMPH treatment, except HO-1. A slight but significant increase of\r HO-1 by AMPH treatment indicated that AMPH may cause oxidative damage in brain.\r These results suggest that the injury induced by long-term AMPH exposure warrants our\r further concerns and investigation.
聽聽貝多芬作品的下一代:將碎形及基因演算法應用於數位音樂產生器
本研究整合了碎形圖形的迭代運算方法與基因交配觀念來達到音樂創新,並透過音樂和諧性判別機制來提高創新音樂的悅耳程度。利用基因觀念之交配的方法來解決長短的問題。這個方法是把原始音符輸入後,找出它們的中心點,以這個中心點為準,其他的音符按照一定比例向外延展,成為新的迭代點。再利用這些迭代點,迭代出新的音符。把製造好的音符染色體放置到交配池中,以隨機的方式在交配池中選取其中之一個染色體進行交配的動作,此二音符染色體會交換彼此的基因,產生下一代新的代表音符長短之染色體,隨後以「模仿母體判斷式」來判斷這新一代的音樂是否與母體音樂相似,藉此淘汰掉「不肖的」下一代,而若新一代與母體的相似程度高的話,它的悅耳性相信也會相對提高。最後把這些技術應用於數位音樂創作,以衍生新穎應用與創新的結果。Fractals can be produced by IFS (Iterated Function Systems). By iterative computation of many times, we can obtain the similar graphics. In my research, the methods to generate the iterative algorithms were presented. In addition, I would discuss the regularity and the content as well as the properties of those digital patterns. At last, the advanced application of fractals to digital music pieces was presented. The program took a note of several measure of music as the beginning point, and made the IFS calculations for each new note in each measure. But there was no difference in beats if you just make the IFS iteration. So I changed the beats with genetic crossover method. In this research, the expression of the DNA to each beat of note was adopted. The same way, it took a note as a beginning point. And the system obtained the new DNA from the old notes for new ones randomly. After producing the new pieces of music, I want to know if it is good to listen. So I used the algorithm that checks the simulation to the shape of mother music. If its shape is similar to the mother music, the probability that the new music is pleasing may even increase. That would make a piece of brand new music. What I want to do in this research is improve the multiformity of music and find what the relationship is of ‘good music’ and mathematical algorithms
溫差電池的熱力學研究與應用
溫差電池中若僅進行的反應,則其電池電壓與溫差成正比,且純粹是利用化學反應將熱能轉換成電能,我們稱之為「典型溫差電池」,由熱力學公式可推導出典型溫差電池的電動勢(ΔS = S(s)—S(aq),S為絕對熵, n為得失電子數,1F = 96487 C ),且得到下列三項推論來說明溫差電池的特殊現象。 (1) 同一溫差電池,其電動勢與溫差成正比 (ε∝ ΔT)。(2) 不同的溫差電池,當溫差一定時,電壓ε 與ΔS 成正比,與得失電子數n 成反比。典型溫差電池中,電解液濃度越小,金屬離子濃度也愈小,會使得ΔS = (S(s)—S(aq))的絕對值變大,因此溫差電池的電壓也就愈大。(3) ΔS 值的正負決定電壓ε 的正負。Cu(NO3)2 及ZnSO4 溫差電池的ΔS 為正值,所以高溫杯為正極;AgNO3 溫差電池的ΔS 為負值,所以高溫杯為負極。因水溶液中陰、陽離子不能單獨存在,所以單一離子水溶液的絕對熵無法求得,但科學家把氫離子水溶液的標準絕對熵定為零,藉以求出其它離子的絕對熵,然而我們測得在一定溫差時典型溫差電池的電動勢ε,再查得金屬的標準絕對熵 S(s),代入S(aq) = S(s) — nFε/ΔT,便可得到離子水溶液的絕對熵。Cu(NO3)2 溫差電池的電解液中若含有1M 或0.5M 的KNO3,電池電壓仍然與溫差成正比, 但卻可獲得較大的電流,我們稱此類溫差電池為「改良型溫差電池」。我們利用改良型溫差電池的原理,自製環保、節約能源、可重複使用的實用溫差電池,以PVC 水管當容器,上、下兩端開口用銅片封住當電極,管內裝海棉及0.125M Cu(NO3)與 1M KNO3 溶液,熱源加熱上層銅片形成溫差,當溫差維持在70℃,電壓約為70 mV,若串聯30 個實用溫差電池,電壓可達2 V 以上,就可以對鉛蓄電池充電。實用溫差電池的熱源可由回收冷氣機、工廠的廢熱,或直接利用太陽能來當熱源。
If the temperature difference cell only goes through the following reaction Then the potential created by the cell is proportional to the temperature difference, and such a reaction purely changes the thermal energy into electrical energy through chemical reaction, which we often name it “typical temperature difference cells”. We can come to the following formula for the typical temperature difference cells through a series of thermodynamic formula: ε= ΔT . ΔS/ nF (ΔS = S(s)—S(aq), where S is the standard 3 entropy, and n is the number of electrons gained or lost, and 1F = 96487 C). We also provide the following three inferences to demonstrate the special phenomenon for the temperature difference cells: 1. Within the same temperature cell, the electromotive force (EMF) is proportional to the temperature difference. 2. When the temperature difference keeps constant, the electromotive force is proportional to the ΔS in different temperature cells, and is inversely proportional to the number of electrons gained or lost. Within the typical temperature difference cells, when the concentration of the electrolyte becomes more diluted, the concentration of the metal ions also proportionally become lower, which will make the absolute value of the following equation bigger, as a result, will make the electric potential of the temperature difference cells bigger: ΔS = (S(s)—S(aq)) 3. The value of ΔS decides the value of the electromotive force. The ΔS of the following temperature difference cells is positive value: Cu(NO3)2 and ZnSO4 . As a result, within the copper and zinc temperature difference cells, the higher temperature glass is the anode. On the other hand, the ΔS of the AgNO3 temperature difference cell is negative, which means that within the silver temperature difference cell, the higher temperature glass is the cathode. Meanwhile, because the cations and anions can not exist alone, therefore, it is not possible to find the standard entropy of the single ion solution. However, scientists define the standard entropy of the solution containing hydrogen ion to be zero, as a result, we only have to determine the electromotive force for a typical temperature difference cell, while keeping the temperature difference constant, followed by finding the standard entropy for the said metal S(s). Inserting it into the following equation to find the standard entropy for the ion solution. S(aq) = S(s) — nFε/ΔT If the electrolytes for the Cu(NO3)2 temperature difference cell contains 1M or 0.5M KNO3 , the electromotive force is still proportional to the temperature difference, and we can obtain bigger electric current. We call this kind of temperature difference cells “improved version of the typical temperature difference cells”. We try to make more environmental, energy saving, and recyclable temperature difference cell by applying the theory of the improved version of the typical temperature difference cells. We use PVC water pipe as the containers, both edges of the pipe sealed with copper metals, also work as the electrodes. Within the pipe filled with sponge and 0.125M Cu(NO3) and 1M KNO3 solution. The heat source keeps heating the upper copper metal to keep constant temperature difference. When the temperature difference is kept around 70℃, the electric potential is 70 mV. If we can connect 30 practical temperature difference cells in a series, the electric potential will reach 2V, which can then charge the lead rechargeable battery. The heat sources of the practical temperature difference cells can be supplied by the recycled air conditioners, heat waste from a factory, or directly comes from the solar power.
垂直水柱的成節機制探討
本研究欲探討垂直水柱遇障礙物成節的形成機制。以數位照相機、光電計時器等進行觀測。 實驗結果如下: (一)因往返水柱波速不同,而且節無波腹大幅振動現象,故節不是駐波現象。 (二)細針插入水柱表面時,當針上方超過某長度後,針下方產生V字形震波。但不論針相對水柱的速度是否超過波速,針上方都有節,故不是震波所產生的現象。 (三)根據水波槽模擬實驗,不論木條是否超過波速,木條前方均產生波紋。木條前方的水受到木條推動,往前方加速,因此顯現出波紋了。 我們認為,在水柱中所看到的節,不是震波或駐波,而是相對於木條往前傳遞的波。波源是撞擊物,改變了水柱表面的壓力,而成為波源,水柱的水因受撞擊,某個範圍內流速會小於波速,使得撞擊物前方存在波紋。This experiment uses digital camera and photoelectric timer to discuss the mechanism of causing spouts to form nodes on its surface. Because the downward wave velocity of the spout is different from that upwardand there are no significant vibrations of antinodes, standing waves are not the mechanism of causing nodes. In the experiment of inserting a needle into the spout, we found out that while the needle was inserted above a certain length of the spout, v shaped bow waves emerged. However, no matter the velocity of the needle related to the spout is over the wave velocity, there are always nodes above the needle. Therefore, bow waves are not the mechanism of causing nodes. According to the ripple tank simulating experiment, no matter whether the speed of the wooden stick is faster than the wave velocity or not, there are always waves forming in front of the wooden stick. The wooden stick pushes water in front of it and causes the water to accelerate forward. Therefore, waves appear. We think that the nodes we see on spouts are neither standing waves nor bow waves. The nodes are rather caused by the relatively moving wooden stick. The object, which impacted the spout (wooden stick), changed the pressure of the spout’s surface and became the source of wave. Because of the impact, the velocity of the water current of a certain area became slower than the wave velocity and causes nodes forming on the surface of the spout.