Blood Brain Barrier Breached!
The purpose of this project is to determine if it is possible to use Ascorbic Acid Sodium- Dependent Vitamin C Transporter Type II, SVCT2, as an effective and safe protein to attach to certain brain tumor treatments to bypass the Blood Brain Barrier (BBB). Stemming from this problem, a procedure was created to use in vitro engineering with the aid of a professor at the University of Calgary to combine SVCT2 and three specific tumor treatments; Imatinib Mesylate (STI-571), Temsirolimus (CCI-779), and Suberoylanilide Hydroxamic Acid (SAHA). Following this, a metabolic barrier had to be created to simulate the BBB. To do this, the use of three enzymes were mixed and held together using specific bonds. Finally, a special bio-tracer was placed within the barrier to detect any toxic effects that may be produced. Then two trials were made with each treatment on the barrier at 34°C, 37°C, and 39°C. Once this was done observations could be made. When the newly isolated SVCT2 attaches to the three cancer treatments, they would all be able to connect and form bonds with each other. Once the incubation period is over for the first trial at 34°C, 37°C and 39°C, several things would be observed within the data. When counting the number of cells that were able to get into the engineered metabolic barrier, it could be seen that there was a dramatic increase in the number of cells in the 37°C range. SVCT2 can be a powerful tool in combating cancer. Because of its specificity, it may prove to be more advantageous over the currently used drugs which may have unwanted toxic side effects on the CNS. In the near future, SVCT2 could have the potential to be adopted as a promising therapy against cancer and certain tumors. Furthermore, SVCT2 has the potential to be applied to many situations and can be modified to fit a number of situations that deal with getting past the Blood Brain Barrier. Initially, SVCT2 was only modified with three forms of treatment for Glioblastoma Multiforme, STI-571, CCI-779, and SAHA, however there are countless other treatments that have been developed, but that are not in use due to the BBB. This project was successful in determining an appropriate temperature of 37°C for the procedure to be used. The limitations of this experiment include the fact that this experiment was performed in vitro and so complexity among individuals cannot be analyzed. However, this is an early step for the future of SVCT2 as a treatment, and clinical trials to test SVCT2 in vivo may not be too far off.
SMS Link-Interactive
SMS.Link-INTERACTIVE is a system that facilitates the exchange\r of information between a central computer server and a remote cellular\r phone user, and allows remote modification of data in the computer\r through SMS. A cellular phone with a GSM modem is connected to the\r computer physically via a data cable. A program in the computer is\r notified whenever the cellular phone receives new messages, processes\r new messages by first authenticating the user’s identity, then checks for\r the information being asked for, as well as the changes that the user wants\r to be done. Information is then retrieved from a database in the computer\r and the appropriate changes are made. A message containing the\r requested information and a list of changes done is then sent to the user\r through SMS.\r Tests were made to get the speed of the system. Accuracy tests were\r done to determine if the program was able to ascertain the validity of the\r user correctly, return the requested data, and change the specified\r information.\r Using this system, doctors can access a patient’s data and change\r prescriptions; teachers can correct errors in grades; clubhouse personnel\r can add memberships; all these from a remote location by sending a SMS\r request to the cellular phone connected to the server.
Technology of web site advancement
Internet by its content represents a fountain of information, while from the point of view of its arrangement it is a huge dump. There are an enormous number of web sites. Multiple web sites are commercially directed, i.e. are aimed at profit earning. As profit depends on the number of visits to web site, no visitors means no profit. So, to obtain more orders, web site producers should first of all ensure good inflow of visitors (web site attendance). Every year this task becomes more and more critical for commercial web site owners (and not only for them), as the number of similar content web sites increases steadily along with competition intensifying correspondingly. The process of establishing conditions to attract more visitors is called web site advancement. The present paper discusses various ways of how to increase the number of web site visitors, it also describes the particular process of "Theater to Children" (www.teatrbaby.ru) web site advancement. Based on the paper outcomes a CD multimedia manual "Technology of web site advancement" has been developed that will help web site producers to achieve good attendance for their network resources. As the purpose of web site advancement is visitor number increase then the main criterion of web site advancement efficiency should be the number of visitors for a certain time period, e.g. for 24 hours, a week or a month. Taking into consideration that about 80% of Internet users retrieve information through search systems, the major growth of visitors will occur owing to the enhancement of web site visibility in search systems.
Problems of Safe Storage, Collecting and Recycling of Luminescent Lamps
Our research is connected with the problem of the mercurious wasre products (MWP) recycling, luminescent lamps take a great part among them. The problem of recycling MWP is topical not only in Yakutsk, but also in other cities of Russia because of its toxic influence on the human’s body. Mercury is the top among eight the most dangerous metals. I have analyzed the conditions of recycling of luminescent lamps in Yakutsk schools. I have got data on the problem. There is a great amount of the fused luminescent lamps is stored in the territory of schools, it produces danger for the pupils’ health. On the research work I have proposed some ways of solution of the problem.
Tamarind(Tamaridus Indical.)Seed Coat Extracts As Coconut Oil Antioxidant
After short period of storage the coconut oil at room temperature we found that it becomes rancid. The oxidizing flavor is disgusting and causes economic loss. Some chemicals are used to prevent the oxidation of coconut oil but they are expensive and may be harmful to consumer’s health if used daily. Moreover, they are not suitable for homemade coconut oil and residual waste from the process may be environmental toxic. The present work thus aims at extracting substances from local plants and used as antioxidant for coconut oil. Seven kinds of local Caesalpiniaceae plants in Eastern Thailand namely Tamarind (Tamaridus indica L.), Peacock flower Caesalpinia pulcherrima L.), Flame tree (Delonix regia L.), Golden shower (Cassia fistula L.), Siamese cassia (C. siamea Lamk.), Candelabra bush (C. occidentalis L.), and Copper pod (Peltophorum pterocarpum DC.) were used. Ten grams of seed coats were mixed with 50 ml of the distilled water and 95% Ethyl alcohol (1:1) mixture in a closed container. The mixture was heated in a water-bath at 60 ℃ for about 2 hrs. and then kept in 10 ℃ icebox about 2 hrs. The mixture was then filtered and the filtrate was heated in an hot-air oven at 80 ℃ for about ? hrs. Ten ml of coconut oil was added with 0.5g of the extracts and kept at room temperature for 5 days. The pH and peroxide value (using the Iodometric titration method) of the oil were measured. It was found that the coconut oil with the Tamarind seed extract had the lowest peroxide value and was selected. Next the suitable amount of the Tamarind extract was studied by adding the extract to 10 ml coconut oil at 0-7% (w/v) and the contents were kept at room temperature for 5 days. The result showed that the 3% (and more over) Tamarind extract had the lowest peroxide value. Then the coconut oil with 3% Tamarind extract was kept at room temperature for 30 days. The pH and peroxide value were measured every 5 days. It was found that the oil could be stored for 25 days without significant change in pH and its peroxide value was less than 10 milliequivale n t oxygen per kilogram oil according to FAO/WHO (Codex) standard .Therefore, Tamarind seed coat at 3% could be used to retard the coconut oil oxidation for 25 days. This work presents the applicable use of plentiful local plants such as Tamarind seed, which is normally discarded, as an antioxidant for coconut oil.