二等獎

Feeding behavior is the action which animals depend on maintain livelihood. Snakes usually use the three following ways to catch their preys: winding, venom-releasing and pressing their game to death. However, previews study is rare about the feeding behavior of Ramphotyphlops braminus. This puzzles us, prompting us to do in-depth research on it. When performing an experiment, we will use the camera with infrared function to record entire experiment and the obtained data will transform the graph. Our result shows the feeding behavior of R.braminus is a new way to catch their game, and the minute process of this special way is also written down in our report. We hope that the result can let everyone be clear about Ramphotyphlops braminus of soil ecosystem status, and it is an essential contribution for building the archive of Family Typhlopidae. 攝食行為是動物賴以維生的行為。在蛇類中，常見的捕捉方式有：纏繞、 使用毒液、壓斃等三種類型。但，文獻中卻沒有任何有關於鉤盲蛇(Ramphotyphlops braminus)的捕食行為。這使我們感到疑惑，並想深入探討。在實驗進行當中，我們使用紅外線攝影機將實驗全程錄製下來，並將所得的數據轉化成圖表。而其結果顯示鉤盲蛇(Ramphotyphlops braminus)的捕食行為是一種全新的模式，這種模式的詳細過程也被我們全部收錄到報告中。我們希望做出來的結果能讓大家對鉤盲蛇(Ramphotyphlops braminus)在土壤生態系中的地位更加了解，而且對於建立盲蛇科(Typhlopidae)資料庫有實質的貢獻。

震盪反應有許多種反應類型，至今已被完整探討，但對於震盪反應之催化系統，卻鮮少有文獻提及，本研究小組偶然發現，醇類對於震盪反應具有明顯的催化作用，本文嘗試探討各級簡單的醇類對於震盪反應影響，並透過活化能的改變了解醇類在催化過程中所扮演的角色。The many types of BZ oscillation reaction have already been thoroughly discussed. However, little mention has been made in literature regarding the catalysis system of the reaction. The researchers have accidentally found that alcohols exert an obvious effect on the oscillation reaction. The study attempts to discuss the different influences that various kinds of alcohols have on the oscillation reaction and to understand the role alcohols play in the catalytic process through the change of activation energy.

1. Purpose of the research\r Nowadays, many encryption software are available to computer user. However, very few people use them which result in many cases of information and data leakage due mainly over the Internet. Most people use USB Flash Drive to store and carry confident files back home to work on. When the USB Drive is lost, the files stored are often leaked. To prevent this type of incident occurring, “CryptoDefender” is developed to enhance files protection and to change user’s habit in handling confidential information. \r 2. Procedures\r “CryptoDefender” is an add-in for common application software, e.g. Word, Excel, etc. It will appear as a new tab in the ribbon which provides the software an alternative for saving and opening a file. It protects the files in two ways: encryption and steganography. The data is first encrypted into cipher text as these encrypted data will be hidden in an image file to lower suspicion of readers. All of these procedures will be done by one click. For encrypt and decrypt, the user just needs to click the “Protect” and “Decrypt”. An extremely fast way to encrypt the file is “Quick Protect”. It will randomly choose an image from the image folder according to their file size. This function saves the time for choosing an image.\r 3. Data\r The method for steganography is using last two bit of R, last three bit of G and B to store the encrypted data. In 24-bit bitmap, RGB is represented the color of each pixel and each of them is 8-bit. The leftmost bit of each byte contains the highest value, and the right one contains the lowest value. Therefore, any change in the rightmost byte will only cause a minimal change in a byte value, and the color as well. 2-3-3 method is adopted to replace the pixels as encrypted data. It is used because it is in coordination with the program’s fluency. 2-3-3 is obtained so that 1 pixel will contain 1 byte of encrypted data. For R, the last two bit will be replaced by encrypted data. For G and B, the last 3 bit will be replaced by encrypted data. If the Bitmap size is larger than the encrypted data, random data will be replaced into the remaining pixels.\r 4. Conclusion \r “CryptoDefender” is user-friendly and efficient way to protect important and confidential digital files on most personal computers. This software will help to change the user’s habit in securing their information as to avoid data leakage even when they are accidentally lost.

本研究嘗試以沒有經濟價值之農業廢棄物，經由化學反應轉換為氫能源，並藉由化工程序來達到連續製程。 我們用爛掉的香蕉為基質，從校園及生活周遭的環境尋找產氫微生物，發現可生產氫氣之微生物隨處可得。在培養溫度35℃下，選擇不同濃度之基質，發現基質濃度與產氫量成正比，且產氫微生物適合在弱酸（約pH=5.5）環境中生長。 以此條件進行連續式反應，當反應槽體積為700ml時，經過26小時後，槽內基質消耗殆盡，此時開始連續進料，當進料流速為138毫升/小時，則水力停留時間為11.67小時，產氫量為5.43升/天，最後連接氫燃料電池，可得到連續產生的電能。另外本實驗也以爛木瓜進行批次反應，同樣也能獲得氫氣。 經由實驗結果可得知，利用農業廢棄物為基質，可運用化工程序發展為連續產氫製程，且應用於實務上，能提升全球環保的概念，我們期待能開創一個能取代「碳經濟」的「氫經濟」。

Methods of nonlinear time series analysis were compiled for use in the analysis of Electroencephalogram (EEG) tracings of children aged three to seven with varying degrees of autism in order to provide a quantitative means of diagnosing autism and determining its severity in a child. After determining the EEG leads to be used for analysis, the identified methods were coded and saved as functions on Scilab. To test the compiled program, a minimum of five EEG readings per cluster of children diagnosed with mild, moderate, severe and no autism will be obtained. The project was able to identify the mean, standard deviation, skewness, kurtosis and other higher order moments, the autocorrelation function, and the Fourier Series as the time-resolved statistical methods to be used for time series analysis. The nonlinear analysis methods identified include the use of the correlation integral, time-delay embedding and the Lorenz equations. One-way ANOVA testing will then be used on the numerical data obtained from the analysis to determine if a significant numerical differentiation has been obtained between the different clusters of EEG. This will provide a definitive way to medically diagnose autism, pinpointing children afflicted with the disorder and giving them proper treatment.\r Two copies of the "Abstract of Exhibit" (in English) should be sent to the National Taiwan Science Education Center or email to fung@mail.ntsec.gov.tw or yuonne@mail.ntsec.gov.tw before December 31, 2009.

Nicholas’s project is a transforming robot, who he has named Reggie. He's about a foot tall and can transform from a biped into a vehicle and vice versa?\r Reggie has been Nick’s project for just under a year now, mainly working on him in technology classes. He operates without physical connection to anything else, so everything he needs from micro controllers to power is stored somewhere on Reggie’s body?\r Reggie is controlled through a programmable Arduino board (programmed in C++) that is connected up to a motor driver, for controlling the two driving motors and an SSC-32 servo controller for controlling the 14 servos via serial port. The Arduino uses the SSC-32 to coordinate the servos movements using pre-programmed sets of movements stored in procedures in order to complete all actions.\r All the parts were produced on a 3D CAD system to begin with, as well as the robot as a whole, although the design did change drastically over the course of its construction and now is considerably different from the original design as there were many problems and complications ranging from space to torque issues that called for a change in design.\r Most of the parts were from the servo erector set from lynx motion, although some of them were used in the way they were intended to and most of them were modified slightly. Some parts Nick manufactured himself either on a rapid prototyping machine, a lathe or milling machine and sometimes a combination of them or even high temperature soldiering?\r Currently Reggie is capable of driving and standing up and is currently radio controlled. He will eventually be capable of walking and modifications to allow him to (such as stronger leg servos) have been made to facilitate that, but it is possible more may have to be made.

特定基因的表現與不同氧自由基的產生，已知會影響細胞的生長和死亡。我\r 個人有興趣利用間質幹細胞體外培養擴充，以為筋骨組織再生的可能應用。因此\r 利用轉植(transfect)突變ras 基因(Glu61Leu)進入骨髓間質細胞株(HS-5 cells)，來\r 控制ras 基因表現的高低，進而研究這些間質細胞隨著Ras 蛋白質表現的高低，\r 對氧自由基引導細胞生長與死亡之影響。結果發現ras 基因高度表現的間質細胞\r 生長減緩；相較於原生株平均減少62.4%。進一步研究其生長減少是否與細胞凋\r 亡有關，發現ras 基因高度表現的間質細胞凋亡確實比原生株高22.6%。探究其\r 凋亡原因，發現與caspase-3 有關但和粒腺體功能無關：因為caspase-3 有活化，\r 但是以粒腺體膜電位螢光追蹤劑JC-1 測得的膜電位卻沒有改變。追蹤ras 基因高\r 度表現的間質細胞其細胞內氧自由基的產量，發現Ras 高度表現株其細胞內氧自\r 由基明顯增加。當細胞外加入超氧根轉化酵素(SOD, 500 U/ml)去清除超氧根時，\r 對Ras 高度表現細胞的凋亡沒有影響；但是外加觸化酵素(catalase, 500u/ml)於培\r 養液中，卻可以抑制ras 基因高度表現的間質細胞內caspase-3 活化和細胞凋亡；\r 並且增加細胞生長循環促進分子Cyclin D1 的表現。從這些研究我們歸結出兩點\r 重要新發現: 1) ras 基因突變造成Ras 高度表現時，會促成細胞內特定氧自由基產\r 生，使得細胞生長減緩並進行細胞凋亡，只有特定抗氧化酵素(catalase)才能恢復\r 其異常； 2) Ras 蛋白高度表現而引導氧自由基產生的細胞凋亡與caspase-3 活化\r 有關，但與粒腺體功能無關。根據這些發現，未來我們或許可以朝調節特定氧化\r 還原反應或使用caspase-3 抑制劑去調控間質幹細胞的生長，以供筋骨組織再生\r 的應用。Certain gene’s expression as well as different species of oxygen radicals can\r affect cell growth and apoptosis. We are interested in amplifying mesenchymal\r progenitor cells for the application of musculoskeletal tissue regeneration. Thus, we\r transfected a mutant ras gene (61Glu/Leu) to change Ras protein expression in the\r mesenchymal progenitor cell line (HS-5 cells) and studied how ras expressing levels\r influenced intracellular oxygen radicals, and its relationship to cell growth and\r apoptosis. Results showed that Ras over-expressing HS-5 cells grew slower than those\r with wild type ras HS-5 cells and revealed a higher apoptosis rate. The higher\r apoptosis in Ras over-expressing cells was not related to mitochondrial dysfunction\r since mitochondrial membrane potential was normal as determined by flow\r cytometric analysis of JC-1 fluorescent staining assay. The higher apoptosis was\r related to higher caspase-3 activation. Further studies showed that Ras\r over-expressing HS-5 cells revealed a higher production of intracellular oxygen\r radicals in comparison to those with wild type ras HS-5 cells. Addition of catalase\r (500 u/ml) but not superoxide dismutase (SOD; 500 u/ml) specifically revived the cell\r growth associated with increase of cyclin D1 expression, but decrease of apoptosis\r associated with lower caspase-3 activation. Results from these studies demonstrated\r two important findings: 1) the ras gene over-expressing in a ras-mutant HS-5 cell line\r triggers a higher production of intracellular oxygen radicals resulting in higher cell\r apoptosis; and 2) the higher oxygen radicals related cell apoptosis is mediated by\r caspase-3 but not mitochondrial dysfunction. Based on these findings, we may\r propose to regulate mesenchymal progenitor cell growth for musculoskeletal tissue\r regeneration via modulation of redox reactions or caspase-3 inhibitors in the future.

A program based on the simulated annealing (SA) algorithm was developed to allow for a longer-term prediction of the occurrence of solar flares, thus reducing the risks they may pose to astronauts, satellites, and ultimately the planet Earth. Sunspot data such as the solar cycle, the appearance of sunspots, the sun's magnetic field, the occurrence of solar flares and other factors that concern the sun were considered in the development of the algorithm. The program, coded in C++, included providing for an initial random state that pertained to the sun's state at a specified time. A neighborhood function was designed based on how sunspots are formed and how they disappear, and the probability function was designed using previous solar cycles to show relationships between the number of sunspots and the time it takes for the sun to reach solar maxima. Finally, the cooling function was designed as a representation of the time. Recorded sunspot counts obtained from the US National Geophysical Data Center (NGDC) during the solar cycle 22 from 1986 to 1996 was used as test data. The output was compared to the actual recorded solar events or the period. The program was found to be 66.7% accurate in predicting solar flares when compared on a smoothed trendline, while it exhibited a 50.5% accuracy when comparing day-to-day data. Since solar flare acitivty is predicted in terms of the trend of activity and intensity, improving daily data accuracy is superfluous. Smoothed trendline prediction accuracy may however be improved by altering the importance of each factor in predicting solar flare activity and by devising a different sunspot classification scheme for intensity and activity.

在機械系統中，物件表面常產生磨損，但重要物件的磨損將會危及系統的操作及安全。本研究建立了表面磨損自動偵測警告系統，該系統包含監測系統、無線射頻資料讀取器、被動式無線射頻識別標籤(tag)、及電磁波隔離層等，並採”故障觸發”之方式運作。亦即在物件之表面下特定深度埋設有被動式無線射頻識別標籤，但該標籤外層覆有一層隔離層可隔絕該射頻資料讀取器之電磁波。 當物件表面尚未被磨損到特定深度前，讀取器之電波無法穿透電磁波隔離層而激發無線射頻識別標籤，因此無法讀到識別標籤之信息，系統將判定物件磨損狀況良好。等該物件過度磨損時，會把該電磁波隔離層磨除掉，使讀取器之電波可激發無線射頻識別標籤，而讀取到識別標籤內含之信息，系統因此可判別哪一個物件已有嚴重磨損之狀況，而自動提出警告信號。由於使用被動式識別標籤及故障觸發方法，本系統具有體積小、免電池、免維護、成本低、誤判率低等優點。測試結果以車輛輪胎的磨損為對象，證實本系統可以即時有效的偵測到車胎之過度磨損，保障行車之安全。

高血壓所誘發的機械性展延是造成心肌肥大的基本因子，本實驗即藉由微陣列基因分析法同時大量的分析機械性展延所造成心肌細胞的基因表現。將新生鼠的心肌細胞施以 20﹪的機械性展延，抽取其 mRNA製作成 cDNA 探針與現成的 cDNA 晶片進行雜漬反應 （此晶片上包含了 480個如訊息傳遞、控制細胞生長週期、細胞骨架等的已知基因），在眾多有因為機械性展延而造成基因表現差異的基因中，我們選擇了 eNOS 基因（內皮細胞 NO合成?）進行西方墨點法及 NOS活性和 NO 產生量測定的實驗，進一步證實 eNOS 的基因表現量的確是增加的，此一結果與微陣列基因分析所得之結果不謀而合。 Mechanical stretch induced by high blood pressure is an initial factor laeding to cardiac\r hypertrophy. The use of cDNA microarrays has made it possible to simultaneously analyze\r stretch-induced gene expression in cardiomyocytes. Neonatal rat cardiomyocytes were cultured on\r malleable silicone dishes and were stretched by 20%. We compared the transcript profiles of\r cardiomyocytes under mechanical stretch for 60 minutes by hybridization of cell-derived cDNA to\r DNA probes immobilized on microarrays. The microarrays contained probes for 480 known genes\r including signal transduction, cell cycle regulators, cytoskeleton and cell motility, and so on. Eighteen\r genes were indentified that showed significantly differential expression in response to mechanical\r stretch in cardiomyocytes. Of the represented genes expressed, endothelial nitric oxide synthase (eNOS)\r genes was the most interesting one. Northern blot and western blot analysis further quantified the\r expression of eNOS gene. Mechanical stretch also increased constitutive NOS activity and NO\r production. Our results indicate that mechanical stretch induces eNOS gene expression thus increases\r constitutive NOS activity and NO production in cardiomyocytes.