三等獎

我們的研究重心是設計簡便的裝置來檢測生活周邊的用水 · 利用生物對於環境污染的生理特徵、活動力、忍受極限 … 等變化，作觀察、實驗之後，將紀錄結果分析、做成表格，進而形成明確、簡易的指標，以供給一般人更容易的了解用水的品質。本實驗不必使用昂貴的儀器來檢測河水與用水，成本低廉的水生生物為本實驗的最佳選擇 · 可於任何地點完成，作為大及化的檢測方法，本實驗參考水樣急性檢測方法一水蚤靜水式，以水蚤在不同眾屬離子、酸鹼值溶液中的實驗結果，用以做為分析水質的標準，佐證我們生活圈附近的水質現況。Our point of study is design the simple and convenient device to measure the water with peripheral life. Utilize the living beings to the physiological characteristic , energy of the environmental pollution, stand limit. Wait for and change, after making observation , experiment. noting down the result will be analysed , make into the form , and then form the clear , simple and easy index , in order to supply common people with the quality of easier understanding water. This experiment needn’t use the expensive instrument to measure river and water . the best choice of the experiment that the cost is based on living beings cheap aquatically. Can finish in any place , as the popular detection method. This experiment consults the acute detection method of water sample — Water flea’s quiet ability of swimming. with the experimental result in different metal and ion sour soda value solution of water flea, use the standard taking making as water quality of analysis, prove the present situation of water quality of adjacent place of our life range.

1. 遊戲規則：將1～ 2m × 2n的連續正整數，由上而下、由左而右依序填入 2m × 2n的方格內。操作規則允許將2m × 2n做往右或往左或往上或往下的完全對摺，直到操作至所有單位方格均疊成一行，此同時有數字也由上而下形成一數列。2. 本研究即是探討操作完成的數列之數量與數字間的關連性。3. 我們發現：(1) 數列之數量與巴斯卡三角形有關。(2) 形成的數列必符合內文的 [ R(L) 性質]、 [ D(U) 性質]、[ R&D 性質]、[D&R 性質]。 1. Rules of thegame: Fill in order the continuous positive integers 1~ 2m × 2n, from top to bottom and from left to right in the 2m × 2n check. The operational rule allows a complete fold of 2m × 2n either rightward or leftward, or upward or downward, until all the check units pile up in a line. At the same time, all the integers form a series from top to bottom. 2. This study explores the relationship between the number of the series and the integers after the operation. 3. Our findings are: (1) The number of the series is related to Pascal triangles. (2) The series formed meet the properties mentioned in the study: [the property of R(L)], [the property of D(U)], [the property of R & D], and [the property of D & R].

本研究探討大地震前電子濃度的減少與地震之間的關係，以1999年921集集及日本地區2008/06/13規模7.2兩個地震個案進行檢驗。分別以疊圖的方式累積24小時電離層全電子含量(TEC)，採前15天TEC中位數當作背景參考值，探討電子濃度的增加或減少，且以標準差σ檢驗確認其異常現象的程度。此外為分析太陽活動性對電離層TEC之影響，各選取相同磁緯、相同地方時的一點與F10.7指數相似時期做驗證，結果顯示電子濃度值異常減少可排除太陽活動的影響，而極有可能來自於大地震。

本研究是在探討收割後的綠竹筍(Bambusa oldhamii, green bamboo)的老化(aging)現象。一般人說的竹筍老了，通常是指竹筍的質地變硬，口感變差，此即是竹筍硬化的現象，而硬化的主因可能是竹筍受到逆境 (stress) 的刺激後，影響了基因表現的形態，導致纖維素和木質素的增加。竹筍採收後以不同方式處理，觀察切面的變化後發現，以0.2 M蔗糖水浸泡48小時後的竹筍，其切面比浸泡於水中或置於空氣中的竹筍切面較白，筍尖較綠且沒有枯萎的情況。不管是浸泡糖水、水或置於空氣中，都無法防止竹筍的硬化，但浸水和糖水可延緩竹筍硬化的情形，可見要防止竹筍老化，基本上要從抑制合成纖維素與木質素的酵素來著手。抽取竹筍切面處組織中的DNA並以DNA電泳分析之後發現，竹筍的DNA有被降解成小片段的現象，其大小差不多是180 bp的倍數，可見竹筍遇到逆境時也可能會有類似PCD (programmed cell death, 細胞程序性死亡) 的現象。抽取不同處理竹筍的蛋白質進行2D電泳，比較電泳結果發現，三種處理的竹筍的共同點在於減少的蛋白質幾乎都分布在等電點較低的部分。增加的蛋白質大多數分布在等電點較高的區域，這些增加的蛋白質可能和竹筍老現象與PCD有關。本研究還有兩個方向可以繼續延伸研究，第一個是將2D電泳上有明顯差異的蛋白質色點挖出，進行蛋白質定序，再從資料庫中比對，推測可能是何種蛋白質。第二個是研究抑制竹筍合成纖維素和木質素的?的方法，保持竹筍的口感，使竹筍能成為一種能外銷的食品。 The purpose of this study is to investigate and analyze the aging of the harvested green bamboo shoots. The research focused on how to prevent the aging of bamboo shoots and why green bamboo shoots become aging. The term “aging” means that the taste of bamboo shoots becomes hardness post harvest. At first, we tried to find out an anti-aging method, which is not only to keep the green bamboo shoots fresh, but also delicious. The method was to soak bamboo shoots in 0.2 M sucrose, in the water or without any treatment. After 48 hours, the cutting surfaces of bamboo shoots treated with sucrose were whiter, and their outer sheaths were greener than those of shoots soaked in the water or without treatment. The results showed that none of them can stop hardness. But the aging of sucrose- or water-treated shoots was retarded. The results suggest that inhibition of the enzyme activities involving cellulose and lignin synthesis may be required to prevent the aging of bamboo shoot post harvest. To get insight into the reason why bamboo shoots become hardness, the differences of protein patterns and DNA patterns between aging and fresh green bamboo shoots were analyzed and compared. The DNA from the bamboo tissues near cutting surface was isolated and analyzed by agarose gel electrophoresis. The results showed that DNA from shoots was partially degraded. The fragments appear as a ladder of DNA with sizes in multiples of approximately 180 bp. The presence of the olignucleosome-size DNA fragments suggest that the cells may undergo programmed cell death (PCD). The degradation of DNA was not observed in shoots treated with sucrose. By comparing the results of 2-D gel electrophoresis, it was found that some proteins with low pI values decreased or disappeared post harvest, while proteins with increased levels were detected in the high pI area. These changes in these proteins may result in the aging of the bamboo shoots. Prevention of the aging of green bamboo shoots is not easy. However, I found out from this study that soaking the bamboo shoots in the 0.2 M sucrose was a possible way to preserve them. The cutting surface of sucrose-treated shoots remained white, and the sheaths of the shoots was greener than those treated with other methods. Moreover, the degradation of DNA was not observed. However, it still cannot completely stop the aging of bamboo shoots. Reducing the enzyme activities involving cellulose and lignin synthesis may be a direct way to prevent the aging of bamboo shoots. It seems like there are many things to discover in the future.

古籍上記載山藥益肺氣，養肺陰，且最近研究報告發現山藥含有珍貴的dioscorin，其為山藥貯存養分的重要蛋白質，有抑制胰蛋白水解?之活性。而塵?糞便、屍體中的消化蛋白?會破壞呼吸道上皮細胞緊密連接處引起過敏氣喘反應。我對此甚感興趣，進而利用山藥的萃取蛋白dioscorin、塵?粗萃取蛋白及呼吸道上皮癌細胞A549，藉細胞培養、免疫螢光染色、螢光顯微攝影來實驗山藥是否真能保護上皮細胞緊密連接處。再利用膠體電泳、西方墨點法中抗體的高專一性、二抗的高靈敏度來確知我之前的實驗。另外也用ELISA 來實驗dioscorin或塵?引起A549 發炎的情況是如何。由實驗得知，dioscorin 可抑制及預防塵?引起的過敏氣喘反應；而經由ELISA 實驗發現，dioscorin 對於塵?刺激A549分泌發炎物質Eotaxin 並沒有助長或是降低的效果。It was believed that Chinese yam have benefits in treating asthma. Recently, it was found that the valuable dioscorin can be isolated from Chinese yam. The major function of dioscorin is nutrition-storage, however, it also have activities in inhibiting trypsin-like protease. This inhibitory activities trigger my interests. Because the major allergens, mites, and their stool may destroy the tight junctions of airway epithelium cells through their trypsin-protease activities. I therefore carry out an in vitro study to identify whether dioscorin can be used in protecting epithelium from the attack of mites. My results showed that dioscorin can protect the tight junctions of A549 cells from the attack of mite crude extract protein. I believed that dioscorin can be a good candidate in pharmacology application. The genetic and proteomic information are my further focus. On the other hand, we also investigated the possible activity of dioscorin in inhibiting mites-induced inflammation through using A549 as a model and employing ELISA. We found dioscorin neither inhibited nor enhanced the eotaxin being secreted from A549 under the stimulation of mites.

本研究從理想城鎮(Ideal City)街道開始，討論平面上相異n 點到某一點的最短距離和。經研究後發現：當n 為偶數時，則到相異n 點的最短距離和所形成的區域可能是一個點、一個線段或是一個矩形；當n 為奇數時，則相異n 點的最短距離和所形成的區域將會退化成一個點。此外，本研究將理想城鎮的街道換成正三角形的街道幾何平面，同樣是討論平面上相異n 點到某一點的最短距離和。經研究後發現：當n 為偶數時，則相異n 點的最短距離和所形成的區域可能為一個點、一個線段、一個四邊形、一個五邊形及一個六邊形；當n 為奇數時，相異n 點的最短距離和所形成的區域則可能為點、三角形的情況。假使考量各點重要性的比重，分別加權後再求最小點。研究發現無論在理想城鎮或正三角形幾何平面上，皆可將各點視為多個權數相同之點重疊於此點上，便可利用先前的方式求得最小點區域。透過這次的研究，可以利用n 個相異點到某一點的最短距離和實際應用在貨物運送的問題或是消防設施配置等問題。The present study was intended to start with the Ideal City and proceed to discuss the sum of the shortest distance between a point and n different points on a plane. After the discussion, it was found that if n is even, the formed region could be a point, a line segment, or a rectangle. If n is odd, then the formed region must be a mere point. Further, the current study transformed the Ideal City into the geometric plane of an equilateral triangle. Similar to the previous discussion, if n is even, the formed region could be a point, a line segment, a quadrangle, a pentagon, or a hexagon. On the other hand, if n is odd, then the formed region could be a point, or a triangle. The result of this study, which investigated the sum of the shortest distance of a certain point to n different points can be applied to the real life situation, such as transporting goods or distributing fire control facilities.

A pair of bacterial two-component system RssB-RssA was cooperated into Serratia marcescens for toxicity phenolic compound detection. First step of this study, E coli was used to accept the plasmid and certified by fluorescent. Then transfer the system from E coli into Serratia marcescens. Finally, 7 kinds of chemical, included phenol, benzene, toluene, xylenes, 4-chlorotoluene, 2-nitrotoluene, and kerosene, were used to check the sensitivity of this gene modified Serratia marcescens line. The results showed that this gene modified Serratia marcescens line had good performances and responses to those chemicals. 本實驗是以一受到二元訊號傳遞系統調控的發光基因重組質體，送入黏質色拉雷菌中，並以製備好的菌株進行毒性化合物之測試。在實驗的第一階段，我們將重組質體送入大腸桿菌內，並以其發光的有無來判斷是否達到送入的目的，其後再以電泳法確認各基因片段是否正確。第二階段再以相同的方法將選殖好的重組發光質體送入黏質色拉雷菌。第三階段，以發光重組菌針對酚、苯、甲苯、二甲苯、4-氯甲苯、2-硝基甲苯及煤油進行發光測試。結果方面，我們發現黏質色拉雷菌發光重組菌對於這一系列的酚環類化合物的確具有相當高的敏感度。

牛樟芝(Antrodia cinnamomea)是台灣特有的原生藥用真菌，長期被民間認為具有治療癌症、腹瀉、高血壓或保肝的效果。本研究的目的是探討牛樟芝子實體乙醇萃取物對人類乳癌細胞的抑制效用，乳癌是全世界女性最常見的癌症之一，我們透過比較不同培養時期三個月(AC-3)、六個月(AC-6)及九個月(AC-9)子實體之化學指紋圖譜，並測試它們抑乳癌細胞活性之差異。結果顯示，培養三個月的牛樟芝子實體AC-3抽出成份對乳癌細胞的生長抑制作用最佳；而利用西方墨點法探討牛樟芝對細胞週期或凋亡相關蛋白質的變化，推測AC-3可影響乳癌細胞的細胞週期調控，並誘發細胞凋亡。未來我們將進一步深入研究牛樟芝子實體AC-3之抗癌作用機制，並研究對乳癌細胞轉移作用的影響，找出牛樟芝子實體中主要的抗乳癌活性成分。

掃描式穿隧顯微鏡(Scanning Tunneling Microscope)，是在奈米尺度下觀察物體表面的一大利器。但一台精密的STM，造價動輒數百萬元新台幣，本研究嘗試利用簡單、便宜的材料，製作一台簡易的掃描式穿隧顯微鏡。眾所皆知：掃描式穿隧顯微鏡的關鍵，就是量子力學中的穿隧效應原理，及壓電材料的應用，所以這些主題都是本實驗涉及的內容。除此之外，我們也運用簡單的物理原理，取代價格高昂的儀器，例如：利用彈簧代替昂貴的避震系統等。此研究利用穿隧效應的原理，並進而利用電腦得知樣品表面的結構，以達顯微鏡觀察分析物體之功用。 ;The major purpose of our project is to assemble a simple scanning tunneling microscopy with cheap and available materials for ordinary senior high school students. For example, we use springs for damping instead of electromotive force device. Weweld all the electronic circuit boards for ourselves in order to not only lower the cost but also inspect the inner structure more clearly and precisely. After the microscopy is assembled, we will use it as an appliance to observe the samples of nano-particles. We must repeat this procedure several times so as to make sure that the images we’ve gained is true, not just some disturbances produced by the environment. Finally, the microscopy can be applied to research fields as well as academic courses.

本研究自2005 年12 月開始，以臺北市內湖區大湖自公園死亡的漂浮琵琶鼠魚(Many-rayed Sailfin Sucker Catfish )為主要研究對象，探討外來物種與放生行為對大湖生態系的影響。 研究期間共觀察到死亡的琵琶鼠魚隻數計有1085 隻，可記錄到個體形質資料的隻數為910 隻，背鰭棘數則有11 棘、12 棘、13 棘與14 棘四種，分析四種不同棘數的琵琶鼠魚後發現：在體長、頭寬及吻到背鰭長度均無差異(p>0.05)，因此判斷所記錄的個體應是棘甲鯰科(Loricariidae) Pterygoplichthys 屬中的同一種魚種。由檢視魚體並分析記錄數量與環境因子相關性後推測：琵琶鼠魚死亡主因是劇烈起伏的溫度差異，其次是人為因素的刻意傷害。 所記錄到大湖池塘水棲動物有：環節動物、軟體動物、節肢動物、魚類、兩棲動物及爬行動物等六大類共34 種，其中外來物種有16 種，本地入侵物種有3 種，而這些外來動物進入大湖的主要管道應是由個人的棄養或放生行為所造成。琵琶鼠魚因繁殖能力強、具攻擊性、吞食其他魚種卵塊、可適應高污染水體及垂釣客對魚種的篩選等因素，而成為最具生存競爭能力的優勢魚種。不但造成大湖池塘呈現嚴重魚種單一化，更可由靜止水域大量擴散至流動水域。未來如何將政府相關部門、學術研究單位與民間社團等力量結合，共同防範大湖琵琶鼠魚的持續蔓延、宣導民眾正確的放生觀念、積極改善大湖水體污染狀況，並訂定完整之外來物種移除計畫，以期恢復大湖池塘物種歧異度，都將是刻不容緩的重要生態課題。;This research began in December of 2005. Focusing on dead floating Many-rayed Sailfin Sucker Catfish in Dahu Park, Nei-hu Distrct, Taipei city. We discuss the impacts of Alien and of behaviors of the release of captured animals on Dahu Park’s ecosystem. During observation period, there were totally 1085 dead Many-rayed Sailfin Sucker Catfish, of which 910 bodies were found. The number of the thorn bushes on dorsal fins includes 11, 12, 13,and 14. After analyzing four kinds of different thorn bushes, we find that no differences exist in Total length, Head width, Predorsal length (p>0.05). We hence conclude that the recorded object should be species with identical with Loricariidae Pterygoplichthys. With the inspection of the fish’s body and the analysis of the relevance of the recorded quantity and the environmental factor, we infer that the main reason of the Many-rayed Sailfin Sucker Catfish’s death is violent temperature differences and the second is attributed to human’s intentional abuse. The aquatic animals of Dahu Park amount to 34 kinds and six classes such as Annelida, Mollusca, Arthropoda, Pisces, Amphobian, and Reptilia. Among them, 16 kinds that are Alien and 3 kinds are Native Invasive Species. Intentional abandon and release behavior channel of these Alien to Dahu Park. Many-rayed Sailfin Sucker Catfish are highly productive, aggressive, and adaptable to highly polluted water. Besides, they eat ovums from other fishes. Moreover, they are not the fisher’s preference and hence often thrown back into pond once hooked . Thus, they become the most competitive survival fish species in Dahu Park. The high competitive ability of Many-rayed Sailfin Sucker Catfish causes Dahu Park to present a serious unification of fish species. This serious unification of fish species could also be spread from static water areas of Dahu Park to flowing water areas. According to our research, certain urgent ecological issues in Dahu Park are to take precautions against the spread of Many-rayed Sailfin Sucker Catfish, to promote the correct idea of releasing captured animals, to improve Dahu Park’s water pollution, and to stipulate a complete plan about eliminating Alien. Our research suggest that government’s relevant departments, academic research units, and folk corporations should be cooperated to achieve the above four goals. Once the four goals are achieved, we believe that the fish species of Dahu Park will be full of varieties again.