Utilization of Starch for production of plastic-like material
The research is based on the production of biodegradable plastic-like material by only using household materials. Also, it can be made at home and it causes no harm to the environment. The biodegradable plastic-like materials made by different ratio of amylose, amylopectin, glycerol and water has different use. The finished product has smooth surface, highly transparency and well flexibility. Also, it can support strong load and be able to be deformed under stress. Ratios of components are tested on: 1. Easy to injection mold 2. Flexibility 3. Tensile strength and ductility & 4. Water resistance. It is found that the ratio of tapioca starch: glycerol: water = 1.5: 0.5: 9 can withstand 13N of force and 1.5: 0.93: 9 with high ductility. To improve water resistance, more amylopectin should be added to amylose. The best water resistance ratio is glutinous rice flour: tapioca starch: glycerol: water = 0.6:0.91:0.5:9 can withstand 16N force, while 0.6:0.91: 0.93:9 and 1.35:0.16: 0.5:9 with high ductility. All materials are available in supermarkets. Higher ratio of tapioca starch can produce bookmark, with laminate effect. More tough, higher ratio of glutinous rice flour can make cups, spoons and dishes.
Novel Biotechnological Approach for Recognition and Purification of Antibody: Lectin Affinity Membranes
Immunoglobulin G is a glycoprotein structured molecule that is produced by the immune system and protects organism from harmful effects of antigens. Ig G amount in the blood plasma is an appropriate indicator of; infection, cancer, diabetes, cardiovascular diseases, Alzheimer and other autoimmune diseases. Besides, purification of Ig G used in the treatment of these diseases from naturel sources is carried out at high costs on the World market. It is hard to obtain Ig G in high amounts and without any decomposes, that’s why it is important to develop new systems that will help to recognize and purify Ig G antibody. In this project, my purpose was; recognizing Ig G antibody with efficient, high amounted, fast, easily, with less toxicity, economically and purifying Ig G in high ratios from its natural sources. For this purpose p(HEMA-EDMA) membranes are synthesized with free radical photo polymerization method and characterized according to SEM images, swelling behaviors FTIR analysis and elemental analysis. In order to adsorb Ig G to polymeric membranes; polymeric membranes are activated with silanization agent (IMEO) and derivatized with Con A which is a lectin affinity ligand. In the SEM results it is examined that membranes are in spherical structures. Highest swelling value is determined as 224.8%.Binding of IMEO was demonstrated with FTIR and Elemental Analysis. Optimum conditions for Ig G adsorption to membranes are; 1.5 mg/ml initial Ig G concentration, 30 minutes of adsorption time, pH 4 citrate buffer 37 0C and without any different ion strength. Optimum adsorption capacity is determined as 253.8 mg/cm2 and it is also determined that this value is 7 times higher than nonspecific Ig G adsorption to p(HEMA-EDMA) membranes. Ig G adsorption-desorption cycles (5 times) proved that product is reusable without losing its adsorption capacity. According to the electrophoresis, Ig G could be desorbed in pure form without any denaturation to its structure.
Understanding the Modern Diagnoses of Protein C Deficiency "Pcd" with Unknown Gene Plays a Critical Role in the Inherited Thrombophilia
Protein C deficiency (PCD) is found in 1 out of 200 to 500 persons in the general global population which is also one of the common conditions of Inherited thrombophilia, it’s characterized by an increased tendency of blood to clot in human blood vessels. It is caused by several factors including mutations in the genes involved in thrombin binding, protein c activation and numerous clotting factors. This includes F5 (Factor 5 Leiden) gene on chromosome 1q24.2, F7 (Prothrombin) gene on chromosome 13q34, SERPINC1 (serpin peptidase inhibitor C) on chromosome 1q25.2, SERPIND1 (serpin peptidase inhibitor D) on chromosome 22q11.21, HRG (Histidine Rich Glycoprotein) on chromosome 3q27.3, PLAT (Plasminogen Activator) on chromosome 8q11.21 and THBD (Thrombomodulin) gene on chromosome 20p11.21. In the current study, a three Saudi families with inherited thrombophilia has been recruited to identify the underlying cause of this special condition. Whole exome sequencing, targeting all coding exons of the human genome, was performed using Illumina Nextera library preparation kits followed by paired-end sequencing on Illumina NextSeq500 instrument. Reads quality control was performed and reads were aligned to the reference genome using BWA software. Variants calling and annotation was performed using GATK. All known genes involved in causing inherited thrombophilia All known genes involved in causing PCD were excluded by whole exome sequencing. The genes that were previously reported to be involved in inherited thrombophilia were checked for any causative variant. No mutation has been identified in known genes. identifying a novel gene underlying PCD. The Result of this study will hopefully pave the way to better understanding the disease pathophysiology and help in developing DNA based diagnosis, carrier screening and somatic gene therapy.
The Locus of Mid-Tangent Points of Planar Curves
In this project, we defined a mid-tangent point with respect to a fixed point X and a tangent at a point Y on a planar curve C as a point on the tangent that is equidistant from X and Y. We studied the locus of mid-tangent points of conic sections. We found that the locus of mid-tangent points of most conic sections are non-linear curves. However, we observed and proved by using Euclidean geometry that the locus of mid-tangent points of circles are straight lines. The mapping defined by mid-tangent points was studied further. The similarity between a mid-tangent mapping and a stereographic projection was displayed as a one – to – one correspondence function. We also extended the concept of mid-tangent points to three dimensional space and found that the similarity with the stereographic projection was retained in higher dimensions. Finally, we studied the locus of mid-tangent points of a sphere to create a mapping of the sphere to a plane.