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三等獎

實驗探討奈米氣泡水溶液的物理性質

將氮、氧、二氧化碳等三種氣體,分別注入水中成為飽和水溶液,將氣體水溶液分裝入塑膠試管中,用超音波洗淨儀以42kHz的頻率振動,可形成奈米氣泡水溶液。利用奈米粒徑及界面電位量測儀測量氣泡的尺寸以及界面電位後,作實驗探討不同尺寸的奈米氣泡對水溶液的磁性、表面張力、折射率、黏滯係數、以及擴散係數等物理性質的影響。 實驗得知:(1)氮和二氧化碳的奈米氣泡的尺寸愈小,水溶液受到的磁力愈大,氧氣則相反。(2)三種氣體水溶液的表面張力都是奈米氣泡尺寸愈小,表面張力愈小。(3)三種氣體水溶液的折射率都是奈米氣泡尺寸愈小,折射率愈大。(4)三種氣體水溶液的黏滯係數都是奈米氣泡尺寸愈小,黏滯係數愈小,110nm的二氧化碳奈米氣泡使水的黏滯係數降低38.8%。(5)三種氣體水溶液都會阻止甘油在水中的擴散,使其擴散係數變小。

一個不定方程q=a2+b2+c2/1+ab+bc+ac整數解的探討

How to spill your coffee

We all do it – walk along with a cup in hand, and carelessly spill it. While it’s usually more annoying than anything else, it happens to affect almost all of us, and little is done to minimise the likelihood of it occurring. So my aim was to explain the physics behind why we spill drinks when we walk, and to investigate how we can minimise the likelihood of this occurring. I broke this investigation into two distinct parts, explaining the system of the cup, and explaining the effect of walking. From initial observations, it was clear that the cup was a resonating system. Like any resonating system, the cup has a natural frequency. When the cup is oscillated – moved back and forth – at near this frequency, the size of the liquid oscillations is very large. This is because the acceleration is in phase with the motion of the liquid, so in each cycle maximum energy is input into the system. In my investigation I experimentally measured this natural frequency, and created a mathematical model to explain this frequency. It was also found that as the size of liquid oscillations in the cup increases, so does distortion of the fluid surface, possibly enabling spilling. To systematically analyse the effect of walking, I had subjects walk on a treadmill, so walking surface and speed were controlled. However, I also needed an accurate way of measuring the motion of a carried cup. Firstly, I tried to use video analysis; however I found this far too imprecise for measuring small changes in velocity of a cup. In the end I used a smartphone to record the acceleration of a carried cup, as acceleration is what causes the movement of liquid in a cup. This allowed surprisingly accurate measurements to be made, and allowed both the size and frequency of the acceleration to be recorded. In order to relate the system of the cup and the oscillation provided whilst walking I conducted a qualitative experiment into the effect of stride frequency on the likelihood of spilling. When stride frequency was very close to the natural frequency of the cup, spilling occurred almost instantly, while it did not occur if stride frequency was much higher or lower. In the end, my research showed that to minimise the likelihood of spilling your drink walk slowly, use a narrow cup, focus on walking smoothly, and fill the cup well below the rim. Despite this, some people happen to be much smoother cup carriers than others, likely due to their individual biomechanics. And, if you really don’t want to spill your drink, you can always use a lid.

塑化劑對斑馬魚生長發育與活動力之影響

鄰苯二甲酸二(2-乙基己基)酯(di(2-ethylhexyl) phthalate, DEHP)是一種被廣泛使用的塑化劑,具內分泌干擾性質的環境污染物。研究目的是建立斑馬魚模式來檢驗DEHP對環境健康之衝擊。本研究區分為四組,包括DEHP暴露組(10 mg/L與100 mg/L組)與控制組(Fish water與DMSO組)。DEHP暴露10 mg/L組與100 mg/L組的濃度選擇,是參考DEHP污染環境地下水濃度的30與300倍而決定。研究發現DEHP暴露組(100 mg/L)的幼魚身長與後端腹腔有明顯下降(P

Relationship between sexual deprived Drosophila, NPF, and the following behaviors

研究指出,公果蠅在求偶的過程中若被母果蠅拒絕,腦中的神經傳導蛋白NPF(neuropeptide F)會隨之下降,並提升其對酒精食物的攝取量。 我們的研究結果顯示求偶被拒的公果蠅是在「攝食酒精」後才對酒精成癮,而非在求偶被拒之後馬上去攝食酒精。而且,「求偶被拒」的公果蠅除了飲酒行為,也會提升其再求偶慾望。另外,我們利用Gal4-UAS系統調控了公果蠅腦中NPF神經的活性,發現公果蠅的NPF神經活性被抑制時,會出現與求偶被拒的公果蠅類似的行為。 最後,我們使用了NPF的螢光蛋白的抗體來標定NPF神經活性被激發以及抑制時腦中NPF的含量變化。透過這個結果,我們可以確定腦中NPF量的變化可以控制公果蠅對酒精的趨性、求偶慾望以及對酒精的攝食量。

VERMICOMPOSTING-EFFICIENT DAIRY SLUDGE MANAGEMENT

The continued growth of dairy farming in NZ and the move toward keeping cows on stand-off pads has seen a major increase in two significant waste streams, the wood fibre that is scrapped off the surface of the standing pads and the effluent that is now concentrated at the site of these pads. In combination these waste streams offer the farmer an opportunity to recycle valuable nutrients back into the soil as an up-valued soil conditioner. This investigation explores vermicomposting as a tool to efficiently manage these two significant waste streams. Sludge was removed from a settling pond and mixed with a range of carbon products that are recommended by Dairy NZ for use in stand-off pads: wood chips, post peeling, sawdust and also wood shavings (used in calf sheds). The wood fibre/sludge mixtures were assessed on their acceptability to tiger worms (Eisenia fetida) by measuring the pH of the mixture and seeing if they corresponded with the preferred pH for tiger worms. The vertical spatial distribution of tiger worms was measured over a period of 15 days and the rate at which the worms moved into the different mixtures was assessed. The worm mass before and after this 15 day period was also measured to ascertain the mixtures’ ability to support worm growth. Finally, different ratios of sludge and post peelings removed from a calf shed were used in a choice chamber experiment to establish the worms’ preference. Tiger worms were used throughout the investigation as they represent the worm species most widely used in vermicomposting in New Zealand. Tiger worms feed on decomposing organic matter, bacteria and fungi in the upper organic horizon of soil. All of the unused wood fibre and dairy sludge tested lay within the acceptable pH range for tiger worms. Wood fibre exposed to large amounts of urine ie calf shed post peelings, that lie outside the acceptable range can be favourably adjusted with the addition of dairy sludge. All the particle sizes of the wood fibre tested were found to be acceptable to tiger worms and capable of supporting increase in their body mass beyond that of the compost. Due to the observation that the worms did not integrate themselves as fully in sawdust as the other fibres tested it is recommended that further investigation should be carried out before sawdust is used for vermicomposting. While a comparison of the average worm density in each mixture may indicate a preference for post peelings this cannot be statistically proven and more trials are recommended. The preferred ratio within the limits that were tested is 1:3 calf shed post peelings to sludge (41% dry weight). Vermicomposting can therefore be recommended as a possible onsite technology to process the twin waste streams of wood fibre and effluent generated by dairy farms. The next step would be to implement medium scale field trials with a continuous windrow system, testing resulting compost for its nutrient content and then comparing this output to that of current practises

聚乳酸/天然纖維複合材料之研究-探討加入玉米葉纖維對機械性質之影響

本研究以玉米葉纖維做為聚乳酸纖維的補強材料,並以加入的玉米葉纖維長度為操縱變因,探討其對聚乳酸/玉米葉纖維複合材料機械性質的影響。實驗設計以純聚乳酸為對照組,以加入1mm, 2mm, 5mm, 13mm玉米葉纖維的聚乳酸複合材料為實驗組。本研究以拉伸強度和耐衝擊值來判斷機械性質的強度。 實驗數據顯示,實驗組的拉伸強度與對照組差距不大,但在耐衝擊值卻比對照組高出許多。除此之外,拉伸強度和耐衝擊值都顯示加入2mm玉米葉纖維在實驗組擁有最佳的數值。另外,加入越長的玉米葉纖維反而不會擁有較佳的機械性質。未來期待聚乳酸複合材料能夠應用在更廣的層面。

葉酸缺乏及葉酸補充調控乳癌細胞轉化成癌幹細胞並促進體外腫瘤形成

研究指出葉酸營養狀態與乳癌的風險有相互關聯且癌幹細胞是扮演癌症轉移的主要角色。葉酸缺乏和葉酸補充皆能促進癌細胞的轉移。本研究探討葉酸營養不良和葉酸補充是否會促進乳腺癌細胞轉變成癌幹細胞,將乳癌細胞株養於正常葉酸、低葉酸和高葉酸中,藉以模仿癌症病人的葉酸營養狀態。細胞在每個葉酸濃度中培養2、 4 、 8 天後再培養於腫瘤球體培養液中並測量四種幹細胞標記-mTOR,SHH,Sox2和Nanog。結果顯示:低葉酸會導致癌細胞的生長停滯,藉由標記的表達增加顯示長期低葉酸會促進細胞轉變成癌幹細胞。於腫瘤球體培養液時,低葉酸組亦較對照組於體外產生較大及較多的腫瘤球。高葉酸組會加速癌細胞的增殖和向癌幹細胞的轉變且細胞增生較快並表達較多的標記,其腫瘤球體亦是最大和豐富的。總之,低葉酸和高葉酸都增強了乳腺癌細胞的腫瘤生成和轉變成癌幹細胞。

反轉式風力發電之磁浮轉子研究之探討

本研究的風力發電裝置除了在轉子裝上旋翼外,再將定子裝上另外反轉旋翼,並分析單雙組旋翼在不同電阻、風速等變因下所受的影響,以及磁浮軸承的擺動軌跡。以QBLADE軟體設計旋翼,並以飛機木製作。利用送風機產生風能,以自耦變壓器控制風速、可變電阻改變電阻並進行單雙組旋翼測量;用不同水平力施於磁浮軸承,觀察其擺動。最後將測得數據製成Excel圖表,分析趨勢。

揮發性化合物抑制水稻去白化的分子機制

植物受逆境時常釋出VOCs (Volatile organic compounds)。本研究首度發現一種VOC-月桂烯(myrcene)可抑制水稻芽鞘之去白化現象(de-etiolation)。我們分別在螢光顯微鏡與穿透式電子顯微鏡(TEM)觀察水稻芽鞘,發現葉綠素分布與葉綠體分化均被月桂烯所抑制。研究發現水稻在月桂烯處理下去白化現象受抑制,是因葉綠素合成相關基因ACSF被抑制所致。同時有一些參與光合作用光反應、 碳反應基因表現也有受影響。月桂烯影響光合作用相關基因,有三種不同類型:一、黑暗下基因產物幾乎不表現,照光後才累積,累積量受月桂烯抑制,如RCA、 ISP、G3PD;二、在暗處下基因產物之累積就被月桂烯大量抑制,如ACSF,ATPS;三、黑暗下基因產物之累積受月桂烯抑制,同時月桂烯抑制照光後光誘發基因累積速率,如FNR。由基因產物累積量推測轉錄因子MYB-TF1參與月桂烯抑制去白化現象,且推測RCA、ISP、G3PD為其下游基因。我們進一步分析轉譯後修飾,在磷酸蛋白分析中,我們發現月桂烯會抑制35 kD 的phosphoprotein (pp35) 之去磷酸化,這暗示pp35可能參與月桂烯抑制水稻去白化現象。VOCs含天然毒素,可抑制附近植物生長,可為天然農藥。