全國中小學科展

四等獎

Anti-bacterial Crab bio-bandages with Bio-dressings 2.0

Commercially available bandages such as hydrocolloid are neither biodegradable nor anti-bacterial. Chitin is known to be the second most naturally available polysaccharide which could be transformed to chitosan which is known to be anti-bacterial (Hasan, 2018) (Chao, 2019) and haemostatic (Okamoto, 2003) (Hu, 2018). Chitosan can be further converted to hydrogel which is bio-degradable and has good water absorbance. Anti-bacterial crab bio-bandages and crab bio-dressings should be bio-degradable as it took 42 days and a month for complete bio-degradation respectively, so they should be better than commercial bandages such as Nexcare Hydrocolloid as the disposal of anti-bacterial crab bio-bandages with bio-dressings would no longer pose burden to landfilling or threat to our environment. Anti-bacterial crab bio-bandages with bio-dressings are anti-bacterial with degree of deacetylation of DD% (measured using FTIR Spectrum II) 82.6% (due to the presence of chitosan) even without the application of other anti-bacterial agents and hence can provide complete protection of wounds from skin and soft tissues infections and haemostatic (due to the presence of chitosan). After testing and certification based on IS997:2004 and BS EN 13726-1, they should meet many requirements specified. Anti-bacterial crab bio-bandages should be eligible for marketing. Some results were as follows: 1.4 Anti-bacterial effect of crab hydrogels and roasted crab hydrogels Pure chitosan, crab chitosan, crab hydrogels and roasted crab hydrogels showed significant anti-bacterial effect. NO oral bacterial colonies were present in drinking water with crab hydrogels. Thus crab hydrogels could serve as effective anti-bacterial wound dressings. 1.6 Basing on IS997:2004 standard, the load per unit of area of anti-bacterial bio-bandages was 342g/m2 which met the minimum requirement of 36g/m2, the anti-bacterial bio-bandages had stronger tension strength (>20N both in dry and wet conditions) than commercial hydrocolloid. (2.7N dry 2.8N wet) which was comparable with that required (50-67N) and pH of about 7 which met the pH range of 4.5-8. 1.7 The FSA Free-Swell Absorbency of synthetic blood of crab hydrogel bio-dressings was 1.86g per 5cm x 5cm dressing which was much higher than that of commercial hydrocolloid (0.299g per 5cm x 5cm dressing) based on BS EN 13726-1.

搶救生命大作戰 - AI姿態辨識在智慧型高品質CPR訓練引導式教學輔具系統設計之研究

本研究以CPR訓練模型輔具做為研究對象,根據專家說明CPR動作要正確純熟,除了依賴良好的教學輔具系統引導外,其功能上更需要有按壓姿勢的正確判斷,因此本研究藉助科技AI輔助,設計AI姿態辨識的智慧型高品質CPR訓練引導式教學輔具系統,經研究證實系統能逹到: 一、協助學習者熟練CPR的操作流程並解決訓練模型設計問題。 二、成功運用邊際運算功能提高AI辨識的速度。 三、拍肩反應、判斷按壓位置、深度更可利用壓力感測器及超音波感應器進行偵測。 四、能應用AI代替人類專家判斷按壓姿勢之正確性。 五、具專家模式且可獨立操作的CPR引導式教學輔具系統。 期望人人都能學到CPR正確操作技巧及爭取黃金復甦時間,搶救寶貴生命。

利用SLC13A3基因於果蠅體內建構代謝疾病模型

隨著慢性代謝疾病日益普及,藥物的研發及測藥用的生物模型也越來越被重視,因此我們欲建立一個模擬代謝疾病的生物模型,以供藥物測試對象。有研究發現,當果蠅身上發生I'm not dead yet (Indy)基因突變時,會使果蠅在高熱量飲食狀態下延長壽命、降低體重增長,且影響果蠅體內脂質的代謝功能,目前認為這些現象與養分運輸進入細胞的減少有相關。本實驗中我們將存在於人類身上且為Indy基因的哺乳類同源基因Solute carrier family 13, member 3 (SLC13A3)轉殖入果蠅體內,並分別在全身、頭部脂肪體、腹部脂肪體及神經細胞中過度表達後,觀察果蠅的生長與代謝變化。實驗結果發現SLC13A3基因於果蠅的頭部脂肪體及神經細胞表達確實會出現與代謝疾病患者類似的生理現象,如壽命縮短、體重及三酸甘油脂增加等,且此作用機制與AMPK 訊息傳遞路徑相關。因此我們認為SLC13A3基因於果蠅體內過量表達適合做為代謝疾病的生物模型,對於往後藥物開發極具潛力。

CONTACTLESS AND NON-DESTRUCTIVE DETECTION OF CHICKEN MEAT CONTAMINATION WITH LASER SPECKLE METHOD

Harmful microorganisms in food can cause deterioration of human health, poisoning and in some cases even death. Especially fresh meat and chicken products create a suitable environment for the growth of microorganisms in terms of the nutrients it contains, water activity and pH level. For this reason, detection of microorganisms in meat products is an important issue in terms of food safety and human health. In this project, it is aimed to detect live microorganisms in meat products, especially chicken meat, in a simple, non-destructive, non-contact and fast way using laser speckle method. Laser speckle images of healthy and stale chicken meat were taken, contrast parameter and correlation analysis of the obtained patterns were made. It was observed that the contrast parameter for staled chicken meat increased by approximately 3 times compared to fresh chicken. This increase provides an understanding of the difference between contaminated chicken and fresh chicken. Speckle density changes over time in relation to the movements of living microorganisms. Thus, the correlation in laser speckle density patterns taken from contaminated tissues is disrupted. In the measurements taken with photodiode, by analyzing the change of light intensity of the speckle patterns on fresh and contaminated tissues over time, the detection of microorganisms was made easier and more precisely without the need for image processing. The proposed measurement system is a new method that detects meat contamination with laser speckle imaging. It can be developed and made portable and can be used easily in homes. Since it is a simple, non-destructive and fast method, it can be used to determine the shelf life of meat in food distribution places and markets. In addition, it has the potential to be calibrated and used for other food products other than meat products. The system developed with this study is cheap and easy to use, and the laser speckle imaging method is used in a different field other than biomedical, contributing to the literature.

滾動體在旋轉圓盤上運動之軌跡探討(The motion of a rolling sphere on a rotating disk)

球體在旋轉平台上的運動分三階段:進動階段、螺線振盪階段、打滑階段。進動階段、螺線振盪階段為兩個運動模式的疊加:迴旋半徑漸增的螺旋線運動、向平台中心靠近的平移運動。當迴旋半徑漸增至滑動摩擦力的上限值,球進入打滑階段並向外甩出平台。 研究紀錄球體質心運動參數,並以接觸點準靜態理論計算及滑動-滾動摩擦模型進行數值分析,找出各種變因與運動參數間的關係。 結果發現滾動階段中鋼球作迴旋運動的頻率f球和平台旋轉頻率f盤和有正比關係,且比例值和球標準化轉動慣量δ正相關。由滾動階段過渡到滑動階段的最大迴旋半徑Rmax和f球2成反比、和δ呈負相關、和滑動摩擦係數μk成正比。滾動摩擦使球向平台中心靠近,也使迴旋半徑漸增。平台傾斜或呈錐狀時,球體的運動會向水平方向偏移。

以氮化鎵負載之ZSM-5觸媒行甲烷轉化為芳香烴之研究

本研究以沸石觸媒進行甲烷轉化之研究。以水熱法合成的ZSM-5為擔體,藉含浸法合成GaOX/ZSM-5與藉裂解硝酸鎵/三聚氰胺/ZSM-5的混合物合成GaN/ZSM-5。研究發現,這三種觸媒都具有MFI的特徵結晶訊號,且GaOX或GaN都能均勻的分散在ZSM-5內。然負載上GaOX或GaN的ZSM-5酸性基點會被置換,導致酸度降低。於700 oC下甲烷轉化的測試中發現,ZSM-5幾乎無活性,而GaOX/ZSM-5略低於GaN/ZSM-5呈現的轉化率。產物分布上,GaOX/ZSM-5產生大量的積碳;GaN/ZSM-5除了有部分的苯與甲苯外,最多的產物便是乙腈與氰化氫與。乙腈與氰化氫的產生意味著GaN具有活化甲烷的能力。本研究為甲烷轉化提供了一條新的途徑。

三角形可變動的外西瓦線之共點問題

2001 年 Larry Hoehn 提出了 △ABC 的三個旁接三角形的西瓦線之共點性質,近年的相關研究都是探討邊上作正方形或矩形而構造三個旁接三角形。本研究不限於直角,創新探討角度一般化情形。考慮以 △ABC 頂點為旋轉中心,將三邊分別旋轉實數 φ 後,構造出可變動的三個旁接三角形。我們發現可變動的三條外中線交於一點、三條外高交於一點、三條外中垂線交於一點。我們先探討前述三個動點的軌跡,發現著名的 Kiepert 雙曲線,本研究為 Kiepert 雙曲線的新構造法。接續研究任選兩點所構成的直線性質,有趣的是,外高交點與外中垂線交點連線恆通過重心;外高交點與外中線交點連線恆通過九點圓圓心,我們給出共線三點的有向線段比例常數。最後,我們再探討角度與長度同時可變動的三個外西瓦線共點情形。

菌菌有味—真菌及塔粉綠尺蛾幼蟲食草選擇之探討

本研究主要探討塔粉綠尺蛾食草選擇與其葉內真菌之關係。觀察培養四種植物(烏臼、鵝掌柴、白匏子與島榕)葉內真菌,以真菌生長率、菌絲與孢子形態辨識真菌種類。當我們以烏臼葉內間座殼菌屬(Diaporthe sp.)真菌與不同植物放在一起時,幼蟲取食原為非食草的白匏子葉片,而島榕則有嘗試啃食之現象;此外,飼養過程中我們發現取食烏臼的塔粉綠尺蛾幼蟲糞便較其他種幼蟲易長出真菌,進行幼蟲糞便與消化道真菌培養後,皆有與葉內真菌相似的菌體形態。我們推測烏臼、葉內真菌與塔粉綠尺蛾間的交互作用關係之一可能為:烏臼透過葉內真菌誘使塔粉綠尺蛾雌蛾前往產卵,經幼蟲食用葉片後所排出之糞便以作為葉內真菌傳播的媒介。

圓來這麼多變-黑白幻彩

為了解黑白間隔圓旋轉時為何出現彩色圖案?我們在不同種類的光源、不同的光源閃爍頻率、不同圓盤轉速及使用高速攝影來觀察間隔圓旋轉後的影像,尋找黑白間隔圓與出現彩色圖案之間的關連性。我們發現黑白間隔圓旋轉時出現彩色現象時,光源必須閃爍、光源必為線光譜,光源含有螢光物質,並且發現燈管內的螢光物質吸收紫外光後所釋出的白光,可使黑白間隔圓旋轉時出現彩色圖案,而LED燈泡是藉由藍光激發黃色螢光物質後發出的白光,則無法出現彩色現象。實驗進一步發現,若燈管螢光物質的某一光源在發光後至熄滅之間產生發光時間延長的遲滯現象,將會是黑白間隔圓旋轉後所出現的顏色。

3進位Kaprekar變換之結構

b進位的n位數字x,數字x各位數字由大到小排列為p,由小到大排列為q,定義Kaprekar變換T(b,n)(x)=p-q,例如T(10,3)(x)= 954-459。當T(b,n)(x)= x,稱x為Kaprekar常數。 Tk(b,n)( x)=T(b,n)( Tk-1(b,n)( x))= x, k >1時,稱x為k階Kaprekar循環數。本文解答了以下問題: 1. b進位的數字不包含數字b-1的Kaprekar常數的形式。 2. 3,4,5,6進位的Kaprekar常數的一般形式。 3. 對於2,3進位的情形,我們引入三元非負整數的形式來討論Kaprekar變換,轉換成Kaprekar數對(p,q),再進一步,由來探討比值p/q,將Kaprekar變換轉成Kaprekar函數g(x),解決 Kaprekar 循環數的所有形式及解。 最後我們得到Tl(b,n)( x)必是Kaprekar循環數。