全國中小學科展

四等獎

CONTACTLESS AND NON-DESTRUCTIVE DETECTION OF CHICKEN MEAT CONTAMINATION WITH LASER SPECKLE METHOD

Harmful microorganisms in food can cause deterioration of human health, poisoning and in some cases even death. Especially fresh meat and chicken products create a suitable environment for the growth of microorganisms in terms of the nutrients it contains, water activity and pH level. For this reason, detection of microorganisms in meat products is an important issue in terms of food safety and human health. In this project, it is aimed to detect live microorganisms in meat products, especially chicken meat, in a simple, non-destructive, non-contact and fast way using laser speckle method. Laser speckle images of healthy and stale chicken meat were taken, contrast parameter and correlation analysis of the obtained patterns were made. It was observed that the contrast parameter for staled chicken meat increased by approximately 3 times compared to fresh chicken. This increase provides an understanding of the difference between contaminated chicken and fresh chicken. Speckle density changes over time in relation to the movements of living microorganisms. Thus, the correlation in laser speckle density patterns taken from contaminated tissues is disrupted. In the measurements taken with photodiode, by analyzing the change of light intensity of the speckle patterns on fresh and contaminated tissues over time, the detection of microorganisms was made easier and more precisely without the need for image processing. The proposed measurement system is a new method that detects meat contamination with laser speckle imaging. It can be developed and made portable and can be used easily in homes. Since it is a simple, non-destructive and fast method, it can be used to determine the shelf life of meat in food distribution places and markets. In addition, it has the potential to be calibrated and used for other food products other than meat products. The system developed with this study is cheap and easy to use, and the laser speckle imaging method is used in a different field other than biomedical, contributing to the literature.

Anti-bacterial Crab bio-bandages with Bio-dressings 2.0

Commercially available bandages such as hydrocolloid are neither biodegradable nor anti-bacterial. Chitin is known to be the second most naturally available polysaccharide which could be transformed to chitosan which is known to be anti-bacterial (Hasan, 2018) (Chao, 2019) and haemostatic (Okamoto, 2003) (Hu, 2018). Chitosan can be further converted to hydrogel which is bio-degradable and has good water absorbance. Anti-bacterial crab bio-bandages and crab bio-dressings should be bio-degradable as it took 42 days and a month for complete bio-degradation respectively, so they should be better than commercial bandages such as Nexcare Hydrocolloid as the disposal of anti-bacterial crab bio-bandages with bio-dressings would no longer pose burden to landfilling or threat to our environment. Anti-bacterial crab bio-bandages with bio-dressings are anti-bacterial with degree of deacetylation of DD% (measured using FTIR Spectrum II) 82.6% (due to the presence of chitosan) even without the application of other anti-bacterial agents and hence can provide complete protection of wounds from skin and soft tissues infections and haemostatic (due to the presence of chitosan). After testing and certification based on IS997:2004 and BS EN 13726-1, they should meet many requirements specified. Anti-bacterial crab bio-bandages should be eligible for marketing. Some results were as follows: 1.4 Anti-bacterial effect of crab hydrogels and roasted crab hydrogels Pure chitosan, crab chitosan, crab hydrogels and roasted crab hydrogels showed significant anti-bacterial effect. NO oral bacterial colonies were present in drinking water with crab hydrogels. Thus crab hydrogels could serve as effective anti-bacterial wound dressings. 1.6 Basing on IS997:2004 standard, the load per unit of area of anti-bacterial bio-bandages was 342g/m2 which met the minimum requirement of 36g/m2, the anti-bacterial bio-bandages had stronger tension strength (>20N both in dry and wet conditions) than commercial hydrocolloid. (2.7N dry 2.8N wet) which was comparable with that required (50-67N) and pH of about 7 which met the pH range of 4.5-8. 1.7 The FSA Free-Swell Absorbency of synthetic blood of crab hydrogel bio-dressings was 1.86g per 5cm x 5cm dressing which was much higher than that of commercial hydrocolloid (0.299g per 5cm x 5cm dressing) based on BS EN 13726-1.

變動磁場下磁浮振盪的運動分析

此研究期望找到穩定磁浮的方法及探討產生磁浮振盪的變因。首先利用吸附上鐵材的磁浮體,觀察其造成的磁浮減震。實驗過程藉由變動磁場,發現週期性變動的磁通量對鐵磁體的磁化及渦電流產生影響,進而改變磁振盪振幅。本實驗也利用了磁場的橫向位移帶動轉動中的磁浮體,藉此觀察磁浮體轉動對磁浮穩定的影響。研究結果得知磁場的交變頻率越大,會導致磁浮體所受斥力增加且鐵磁體形成的減振效果減緩。而磁浮體的轉速越快,周圍磁路分布越平均,越容易穩定隨載具移動。

頂心三角形誕生的奇蹟

在第 屆科展作品(中華民國第 屆中小學科學展覽會換心手術)有給定了一個新的名詞(頂心三角形):平面上給定△ABC及一點D,分別以A、B、C三頂點為圓心,¯DA、¯DB、¯DC為半徑畫圓,三圓交於三點E、F、G,再以三交點E、F、G為頂點作△EFG,則新△EFG稱為△ABC在D點的頂心三角形,本篇作品主要探討原三角形與其頂心三角形邊長與面積比例關係,並試著利用這些關係求出頂心線以及其他相關性質。 在我們的作品中,我們求出頂心三角形的三邊長為2¯AD sin⁡∠ CAB、2¯BD sin⁡∠ ABC、2¯CD sin⁡∠ BCA,也就是說在原三角形為任意三角形,可以得出頂心三角形的邊長與原三角形之間的邊長關係,我們再進一步利用邊長關係求出頂心三角形對原三角形的面積以及面積比例。我們還發現,當D點在原三角形的外接圓上時,頂心三角形會退化為一直線,稱為頂心線,而此頂心線會通過原三角形的垂心是本篇作品最重要的發現。

彩色二維條碼手持產品開發之探討

QR Code是由黑白模組組成的二維數位條碼,掃描後可讀取儲存的訊息。受限於設計原理,QR Code使用二進位制儲存資料。增加模組數目可增加資料量,但若在條碼內塞進太多模組時,尺寸太小的模組將無法被掃描器讀取。此外,目前QR Code掃描器僅支援單張掃描,並無法應付同時多張條碼掃描的實務需求。 如能克服顏色辨識,理論上彩色二維條碼將能克服現行QR Code的限制,但市面上並無相關產品可供測試。因此本專題設計了一款10×10、具8顏色的"Colour Matrix",並利用Raspberry Pi開發Colour Matrix在手持裝置上運作的軟硬體來進行實驗。此實驗成功利用機器學習演算法在Raspberry Pi上進行的顏色辨識。開發的程式在單張掃描上效能與使用pyzbar辨識QR Code相當;在多張掃描方面,使用pyzbar辨識QR Code的解碼成功率為3.1%,而本專題的方法將成功率提升至92.4%,擴增數位條碼的使用範圍,具商用價值。

癌症治療新利器-奈米氧化鐵藥物磁性複合微粒之應用

溫熱化學治療(Hyperthermic Intraperitoneal Chemotherapy, 簡稱HIPEC),為將化療藥物加熱後灌注到腹腔內殺死癌細胞,但溫熱化學治療只能在開刀時使用,治療次數有限。為達到多次的熱化療效果,本研究開發奈米氧化鐵藥物磁性複合微粒(Nano iron oxide magnetic drug complex particles, NIOMP),可利用開刀時包覆複合微粒於腹腔,術後用磁場加熱,加上微粒化療藥物釋放,達到多次溫熱化學治療。NIOMP以鐵氧化物奈米粒子為核心,海藻酸鈉為外殼體,利用電紡法將前其與氯化鈣交聯產生凝聚性微粒。數據顯示最佳製作條件為16 kv高電壓、距離4 cm、噴速5μm/s,可得直徑大小約為200~450 μm穩定性高微粒。藥物釋放實驗顯示,NIOMP於2~7小時藥物釋放速率較穩定。利用可變磁場之電磁效應加熱NIOMP中氧化鐵磁顆粒,可於30分鐘內快速升溫至43℃。細胞相容性測試結果得知,NIOMP對細胞無毒性。本研究改善HIPEC的治療限制,可多次針對腫瘤患部局部熱化學治療,未來將可改善病人存活率。

摻鈀鹵氧化鉍奈米晶體光催化還原二氧化碳

本研究將以BiOCl、BiOBr以及Pd/BiOCl的晶體製備二氧化碳還原之光觸媒,用以還原二氧化碳,期許能製造出具有經濟價值的還原產物,以緩解全球暖化並利用可再生能源。此外,我們藉由探討此類晶體在二氧化碳還原中的差異,比較加入不同鹵族元素及是否摻入鈀金屬對於光催化還原二氧化碳效率及產物的影響。 合成晶體後,我們透過X射線衍射儀(XRD)、掃描式電子顯微鏡(SEM)、能量散射X射線譜(EDX)進行晶體的鑑定,也確定Pd/BiOCl的晶體結構是以BiOCl為主體,且Pd鑲嵌在其表面。我們也以X光光電子能譜儀(XPS)了解晶體的鍵結型態及推測不同晶體表面的OVs相對含量,並以紫外光/可見光光譜儀(UV-vis)檢測樣品的能隙,推測其光催化性能。 我們發現摻入鈀的BiOCl晶體結構為分散的片狀結構,是三種晶體中唯一的奈米晶體,且能隙為三者中最小(2.46eV)、表面OVs含量也增加;表面附著的Pd奈米金屬顆粒更可以協助主催化劑的電子-電洞對維持分離狀態,促進其光催化效率。 最後我們將合成的BiOCl、BiOBr以及Pd/BiOCl晶體應用於光催化還原二氧化碳,並以氣相層析熱導偵測器(GC-TCD)及氣相層析質譜儀(GC-MS)檢測產物種類及產率,發現還原出的氣體產物有H2、CO、CH4。而總還原產物及含碳還原產物的產率皆以Pd/BiOCl為最高。

Territorial behaviour of the Eurasian Wren (Troglodytes troglodytes) during autumn migration and wintering in the urban environment of Hradec Králové, Czech Republic

In the present paper I studied the winter territories of the Eurasian Wren (Troglodytes troglodytes) in the urban environment of the city of Hradec Králové. The males of this species were detected through the aggressive reactions to the playback of its conspecific call. The Eurasian Wren territories were detected at seven out of the ten observation points in the city. The frequency of the territories decreased during the observation period from October to January and it was also affected by the structure of the biotope. It seemed that the wrens preferred trees and avoided lawns and isolated growths. That illustrates how important it is to maintain wildlife corridors in the cities. Individuals of seven other bird species were seen reacting to the playback of Eurasian Wren songs or warning calls. Eurasian Wren may be applied as an indicator species of the ecological value of the urban environment. Through this species, we can access such quality also for the human inhabitants.

Locus of the Points on Circumference of the n-th Circle that Formed by Moving the Center of any Radius Circles on the Outermost Circumference of Preceding set of Circles

This project aimed to study the motion which occurred from the end point on the circumference of the outermost circle by moving the center on the circumference of a preceding circle and the center of an innermost circle at origin. According to the study, when angular velocity was changed, it caused the different of loci. Based on the above information, finding the locus of the point on circumference of n-th circle that formed by moving the center of any radius circles on circumference of preceding set of circles was studied to get general equation. A set of circle and locus were created with GSP program. First, set the same radius circles on the X-axis with the first circle at origin, then found the relationship that occurred from the characteristics of locus. The result showed that if the ratios of angular velocity are 1:1:1, 2:2:2, 3:3:3, ..., …, n:n:n or 1:2:3, 2:4:6, 3:6:9, …,nw1:nw2:nw3, the characteristics of locus will be the same, while the others will be different. Finally, the equation of locus was found as follow: (x,y) = { ..........see in abstract...........} when .........see in abstract........... Where ri is the radius of i-th circle, zeta i is an angle between the radius of i-th circle and X-axis, wi is the angular velocity, t is elapsed time and alpha i is a starting angle between the radius of i-th circle and X-axis.

Prismalla: Mist water collector

The lack of drinking water in human settlements triggers a series of problems that are linked and affect the development of humanity: health problems, lack of water security for companies, lack of jobs, insecurity, among others. We observe this problem in the communities of the municipality of Las Vigas de Ramírez, Veracruz, where there is a great problem with the water supply, although there is a high presence of mist. Faced with this situation, we undertook the task of investigating a water harvesting method that is easy to implement, operate and maintain. We investigated and analyzed the methods of mist condensation through physical barriers, finding that the polyethylene shadow mesh was the means to achieve this, because it allows the passage of the wind, it is very light, easy to manipulate and above all that it presents the phenomenon of percolation that allows water droplets of various diameters to be accommodated therein. We designed a device that allows to present a mist catchment area through a prismatic structure enabled with meshes and condensed water receivers, portable, easy to use and maintenance and very economical with a performance of 20 liters per day. To achieve our project, factors such as air humidity, dew point, wind speed and direction, height, temperatures and available spaces must be considered.