全國中小學科展

四等獎

草藥對癌症轉移之影響

癌症轉移是大部分癌症治療失敗的主因。癌症轉移涉及癌細胞不正常活化周圍蛋白水解酶並將胞外基底膜基質(ECM)降解,讓細胞得以轉移至其他器官。本研究聚焦於研發可以阻止癌細胞侵襲其他組織或轉移的藥物,並期望能以藥物控制蛋白水解酶的活性,進而達到降低癌症轉移的機會。我們發現兩種草藥萃取物(MSL-G和MSL-H)可有效地抑制肝、肺和攝護腺癌細胞的移動侵襲力。實驗結果顯示,此二草藥萃取物可有效抑制基質金屬蛋白酶(matrix metalloproteinase 2 and 9 (MMP2/9))的活性,並促進金屬蛋白酶抑制蛋白(TIMP1)的表現。因此這兩種草藥萃取物可能促使TIMP1的表現進而抑制MMP2/9的活性,或同時具抑制MMP2/9的蛋白酶活化機制,並指出此兩種萃取物具有開發為抗癌藥物之潛力,計畫將利用動物實驗進一步驗證此藥物對癌症轉移的效用。

Migration Data-Driven Mathematical Model for New City Growth

The growth of a city and the population movement has many correlations. However, the complex interaction causes difficulties in developing a mathematical model needed for analyzing the growth factor of a city and the movement factor of population. The model involving traditional equations cannot explain many phenomenon. The newly introduced data-science suggests possibilities to overcome these difficulties. Particularly, the abundant amount of accumulated data proposes a new solution for the problem we have. Throughout these steps, data-utilizing methodology, such as machine learning for artificial intelligence, are researched and developed with attention. In this research, data about accumulated for previous population movement and city growth are collected, and a mathematical science model based on data is developed to explain population movement and city growth by utilizing data analyzing methods such as machine learning. Especially, artificial neural network and stratified advance learning(deep learning) proves possibilities in solving many problems. This research aims to construct an artificial neural network appropriate for population movement and consequently use it in developing population movement model. Using this model, growth of many existing cities can be explained and furthermore, examining the population movement factor of a city and social factor necessary for city growth become possible. This model is expected to become the tool for resolving overpopulation and predicting and deciding factors needed for a new future city. In spite of decreasing population, it is still important to develop a model for population movement that well explains city growth and environment change.

以魚類耳石探討A.MAMORATA的生長和迴游特性

鱸鰻(Anguilla marmorata)曾經是台灣的保育類動物,雖然於2009年解除其保育類身分,但對於其生活史目前所知仍然非常有限,相關論文也很少。本研究欲藉由鱸鰻耳石上出現的年輪、日周輪以及微量元素之分析,來重建鰻魚的生長及洄游環境史。我們利用台灣大學漁業科學研究所採自菲律賓的野生鱸鰻和海南島的養殖鱸鰻進行分析,並且和已經發表的日本鰻(A. japonica) 文獻比對,以期歸納出鱸鰻的生活史以及棲地利用的特性。本研究的耳石測量在台灣大學漁業科學研究所進行,魚類耳石鍶鈣比則利用中央研究院地球科學研究所的電子微探儀(EPMA) 進行分析。結果發現鱸鰻自海中孵化後約莫經過130天會回到陸地的淡水環境成長,這與日本鰻回到陸地鹹淡水環境成長的特性有很大差別。這項發現對今後鱸鰻保育工作的環境管理措施,具有參考價值。

Plexiglas: from synthetic glass to cationic exchanging resin

Plexiglas is a macromolecule (poly-methyl-methacrylate) obtained by polymerization of the Methyl Methacrylate. Cation exchanging resins have acidic groups such as COOH (carboxyl) and SO3H (sulfonic) which fix metallic cations dissolved in water releasing an equivalent of protons through the following reaction: 2 RCOOH + Me2+ (RCOO)2Me + 2 H+ Regeneration is made treating the exhausted resin with diluted hydrochloric acid (HCl) which moves the equilibrium to the left. The aim of our research is to re-use the discarded Plexiglas by transforming it into a cationic exchanging resin. Alkaline hydrolysis transforms the COOCH3 group into COO– group; the obtained group is then transformed into COOH group by means of a treatment with HCl. After the alkaline hydrolysis spectra of the solid show the characteristic band of the asymmetric stretching of the COO– (1610-1550) at 1567 (1st experiment) and at 1555 (2nd experiment). Instead after the acidic treatment the spectra of the solid show that this band has disappeared. On the contrary the characteristic band of the OH stretching of the COOH group (3300-2500) at 3228 (1st experiment) and at 3200 (2nd experiment) appears. The water hardness, due to Ca2+ and Mg2+ ions, is studied to verify the capability of the obtained resin to capture these cations. For this purpose, some mineral water is percolated through the micro-columns. There are three experimental evidences to validate the hypothesis: EDTA molecule (Ethylene Di-amino Tetra-Acetic acid, disodium salt) to estimate hardness is not required The pH of the percolated water through the column decreases from 8 of the mineral water without any treatment, to 6.3 after the treatment as expected The spectrum recorded in the visible range of the percolated mineral water through the column plus EBT (Eriochrome Black T) indicator is the same as the spectrum obtained using de-ionized water plus the same amount of EBT In conclusion, the study has provided evidence that it is possible to convert Plexiglas into cationic exchanging resin.

使用機械手臂實現黑白棋之人機對弈

由工業4.0智慧生產的啟發,建構機械手臂進行人工智慧黑白棋之對弈系統,透過視訊分析棋面資料,輸入人工智慧黑白棋遊戲判斷。依據結果指揮機械手臂進行落子、取子的動作,人機對奕過程亦會判斷有無不合理的地方,以維持棋奕的規則公平。擷取雲端攝影機的盤面影像,使用霍夫找圓演算法取得棋目位置,透過彩度與明度分析黑子、白子或無子,黑白棋AI程式再透過遊戲樹演算決定落子、取子位置。透過畢氏定理及餘弦定理將棋子位置的立體座標轉成工具座標,再傳送至主控伺服器以指揮機械手臂進行正確的動作。透過減輕重量及使用彈力平衡力矩改善,機械手臂可改善硬體準確度,重現率測試達85%以上。黑白棋AI程式棋力可以與黑白棋app的3級力敵。視訊判斷棋局在調整適當彩度明度後可達100%正確率。透過演算法指揮機器手臂下棋,棋局中完成正確動作可達80%以上的成功率。

螂吞虎嚥-探討嗅覺及味覺刺激對蜚蠊口器、唾腺及砂囊的影響

本研究以不同濃度各種溶液作為嗅覺與味覺刺激,探討人體與蜚蠊的辨識率與口器反應,發現蜚蠊口器的嗅覺受器極為敏感,大致呈現劑量效應(dose response)趨勢,且可偵測人體無法辨識之揮發性極低的物質,且具熱量之營養物質可增強口器的反射行為。此外,利用記錄組織電位變化的方式,我們發現餵食蔗糖溶液時,蜚蠊唾腺放電反應減弱,推測蔗糖會抑制唾腺分泌水樣唾液,避免稀釋酵素濃度影響消化分解;餵食麩胺酸及醋酸溶液時,唾腺放電反應增強,推測其會引發唾腺分泌水樣唾液,稀釋過高濃度的溶液。而餵食蔗糖溶液時,蜚蠊砂囊放電反應減弱,推測蔗糖會抑制砂囊肌肉收縮;餵食麩胺酸溶液時,蜚蠊砂囊放電則增加,推測麩胺酸會增加砂囊肌肉收縮,以利磨碎食物。

Development of new manufacturing method to generate hydrogen energy by using waste silicon ~ Reuse of waste of the semiconductor industry for hydrogen community ~

Because of the presence of an activated multiple carbon-carbon bond, α,β-unsaturated sulfones are high-reactive compounds which are widely used in organic synthesis. These compounds readily undergo the reactions of nucleophilic addition and pericyclic processes. At the current moment, a wide range of 1,3-dipolar cycloaddition reactions with α,β-unsaturated sulfones as dipolarophilic systems is known. However the interaction of α,β-unsaturated sulfones with azinium ylides is less studied and limited to only a few examples. In the present study, the interaction between a number of stable isoquinolinium and pyridinium ylides with aliphatic and aromatic vinylsulfones has been investigated. We considered the regioselectivity of these reactions. Mostly cycloadditions of isoquinolinium ylides to α,β-unsaturated sulfones led to the mixtures of isomeric sulfonyltetrahydroindolizines in different ratios. Also we found several examples of high-regioselective addition. The stereochemical result of the cycloaddition was examined by methods of 2D correlational 1H-NOESY NMR spectroscopy and X-ray crystallographic analysis. The process of formation of major regioisomer of cycloaddition N-phenacylisoqunolinium ylide to ethylvinylsulfone was highly stereoselective. The series of new sulfonyltetrahydroindolizines with potential bioactivity were obtained. The structure of all products was proved by IR and 1H NMR

絲絲入扣-可發光高分子奈米纖維之探討

過量的紫外線易引發皮膚病變。因此,我希望找出一種能將紫外光轉為有益皮膚之可見光的方法。我以可發光高分子奈米纖維為素材,嘗試了兩種不同的方法。第一種為利用可發光之PFO、PFBT高分子來製造纖維。發現藉由添加特殊基團(BT)修飾,可改變纖維放光波長。另一種為使用聚乳酸混合聚集誘導發光特性(AIE)小分子。在比較各配方後,以HPS小分子與PLA 120 mg/mL溶於二氯甲烷:二甲基甲醯胺(7:3)製出的纖維最佳。而經由光譜圖的觀察比較可證明:電紡的拉伸特性及奈米尺寸的高比表面積,更能增加纖維的發光效率。期望未來能將其運用於光療面膜、抗紫外線衣物等方面。

多方塊的塗色問題

在本篇研究報告中,主要討論一個關於多方塊的問題:給定一個多方塊,試找出n的最小值使得在無限大的棋盤上,可以塗上n種顏色並且使多方塊沿格線無論如何放置,都不會蓋到重複的顏色。一開始先以V形三方塊的情況開始討論,之後將單方塊至五方塊的所有情況都有系統地討論完畢。 為了給出顏色數的估計,考慮同時適用於所有k方塊的情況。也就是說,要找到一個塗上n種顏色的無限棋盤使得無論任一個被選定的多方塊怎麼被放置在棋盤上,都不會覆蓋到相同顏色的格子。本篇研究成功地給出了此問題的精確解。 除了上面一種估計之外,本篇研究也考慮了矩形多方塊的顏色數,並試圖以之給出所有多方塊所需的顏色數之上下界。最後我得到k方塊所需的顏色數至多為8(k+1)2/25.

圓圓不絕的三角問題-三角形分割內切圓性質探討

任意三角形我們可以從其中一個頂點作分割線將其分割為兩個三角形,若分割三角形的兩個(子)內切圓面積和與原三角形的內切圓面積相等,我們稱此分割法為分切解。以直角三角形為例,很明顯的,對斜邊作高的分割方式為一分切解,更進一步的研究,我們發現將其子三角形的內切圓位置互換,其所得依然為一合法的解(稱為交換解)。有了這樣的經驗,我們試圖將問題推廣至更一般性的情況,對於任意三角形,我們證明了以一頂點分割至多存在二分切解,進而,在這樣不失一般性的假設之下,我們推導出簡潔的判別式以判斷針對一頂點是否存在分切解;更甚,我們證實了交換解對任一三角形依然成立。最後我們也發展出針對直角三角形之分切解的尺規作圖法。