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環境工程

塑膠微粒對大型蚤生殖的影響

塑膠微粒常見於自然環境中,若攝入塑粒是否會對生物生存造成威脅?本研究以塑粒及小球藻餵食大型蚤(Daphnia magna),以螢光顯微鏡觀察大型蚤腸道並監測小球藻濃度以推測大型蚤攝食情形,並監測新生水蚤個體數。 研究發現大型蚤在濾食中會攝入塑膠微粒,在含0.08 mg/L塑粒的培養環境下大型蚤攝食量顯著減少。在含0.01mg/L的塑粒環境下大型蚤開始抱卵天數有延長、第一子代個體數會減少,且平均體長亦減少。若子代出生即放回無塑環境,可恢復生長情形。 在含0.01mg/L的塑粒環境下,族群大小有顯著減少且在0.08mg/L濃度下的族群在第七天全部死亡,也就是有塑環境對大型蚤生殖及生長確實造成影響。

Process of making a new eco-friendly membrane

本研究目的是為了更了解海藻膠製成的膠膜於食品包裝的應用。我們將此膠膜命名為新型環保非塑性保鮮膜。實驗顯示,環保非塑性保鮮膜的成功取決於甘油和醋酸的關鍵比例成份。我們以適當比例的甘油,醋酸和海藻膠糊液,能夠控制薄膜的流動性和柔軟性。藉由透明度、穿刺強度、拉伸強度和伸長率的物理性能測試,我們的研究證明,新型保鮮膜具有初步商品化之水準。環保非塑性保鮮膜之水活性值遠小於0.6。在實際應用上,「自製新型保鮮膜」包裝功能特性、包裝視覺效果甚佳,並具備優良的冷藏保鮮效果,而且不用擔心因保鮮膜接觸油脂、蒸煮或微波加熱處理所衍生的食安問題。「自製新型保鮮膜」之水活性值遠小於0.6,因此,常溫下具有相當好的貯藏性。最後,經3週掩埋測試,證明「自製新型保鮮膜」之腐敗性甚佳,是一種對環境十分友善的環保材質。

以磁性Fe3O4分離微塑膠的成效與機制探討

微塑膠因為其密度小、表面積大之特性可吸附有害物質,並傳播至各地,而對人體和環境造成危害,故回收微塑膠是科學家研究的重要主題。本實驗發現:在中性50 mL水樣品中回收以砂紙磨製的微塑膠時,加入0.050克的Fe3O4和1~2 mL乙酸乙酯或正己烷後,微塑膠、有機溶劑和Fe3O4可互相吸附,再用磁鐵將三者同時吸出而與水分離,可達到清除微塑膠的效果,不同微塑膠(PP、PET、HDPE和PETG)的清除效果皆可高於89%以上。 而在中性的50 mL水樣品中,回收較大顆粒之微塑膠(顆粒大小介於0.500 mm~2.380 mm)時,加入微塑膠0.5克、水50 mL、0.5克Fe3O4¬和不等量正己烷時,會有不同回收效果,但普遍以不加入正己烷,微塑膠(PP、HDPE、LDPE和PS)回收效果最佳,皆約85%以上。 推測此清除機制為微塑膠可吸附有機溶劑,有機溶劑可吸附Fe3O4,三者混合後即可用磁鐵以磁力將此混合物和水樣分離,達到清除微塑膠的效果。但當為塑膠顆粒較大、重量較大時,則會停留在有機層中,反而難以用磁鐵分開,故以Fe3O4即可達到回收微塑膠的效果。

路燈不要變壓器好不好

路燈若變壓器損壞,會連同高效率LED晶片被丟棄,就像勞斯萊斯的引擎壞了就整台廢棄。本作品思考如何回收LED良品,改造成「不需要」變壓器也能發揮高功率路燈LED的強光照明並長時間使用。 用a-IGZO取代電阻器,從已發表的論文知a-IGZO經紫外線或藍光激活後,有超過一天以上的持久性光電流,可讓LED有更好的發光效率,路燈LED發藍光,且適合放在玄關入口照得到紫外光激活可持續到晚上。LED需要直流偏壓3.7V下工作,用110V交流電除了電壓相差太大,也有閃爍問題。我們巧妙利用路燈LED是集成晶片,經設計可在交流電變換極性時都有LED亮著,再用擴散片(從液晶螢幕拆下)將光弄勻,讓燈看起來是恆亮,材料成本僅需一元(電阻器)。回收再製後,可用在大門入口或走廊下長時間照明,環保愛地球省電省錢。利用Arduino設計監測模組並用wifi傳回數據,可長時間觀測,證明可行。

探討溫度和碳源對Pantoea sp.處理養殖廢水之影響及應用

本研究探討改善冬季養殖廢水中亞硝酸降解不良的問題。潘朵拉菌Pantoea sp.可在冬天生長並降解水體亞硝酸,不同於其他菌其在低溫時生長較好但降解較差,顯示兩者非正相關。進一步得知溫度會影響Pantoea sp.細胞內代謝機制,也發現氨濃度降低時降解能力上升,西方墨點法及酵素活性實驗得知MDH表現量和活性在低溫較高。水體中添加葡萄糖可使冬季亞硝酸降解能力提升6倍,且不影響其生長,與文獻添加碳源會促進益生菌生長不同。比較各式糖類後得知單醣和雙醣皆可提升降解能力,其中單醣較雙醣好,且此做法適用各鹽度環境,而碳源可提升降解能力,推測因其影響細胞內TCA cycle運作。最後實際到戶外採集養殖池水研究,結果顯示成本低的擴培方式可有效降解亞硝酸,對改善台灣冬季養殖廢水水質有高度應用價值。

見「塑」不見「鱗」?-魚鱗環保薄膜的研發及應用

本研究探討富含膠原蛋白的魚鱗,藉由嘗試各種電器及配合熬煮時間長短,萃取出最佳濃度之魚鱗萃取液,並找出最佳成膜配方為魚鱗萃取液20 g、5 %TG酵素水溶液0.8 mL、甘油 1 mL、乙醇 1.5 mL,以冰箱冷藏方式製作出魚鱗薄膜,接著以自製儀器測量魚鱗薄膜之透水性、穿刺強度、彈性、水溶溫度、膠熔溫度等物理性質,再將製作出的魚鱗薄膜包覆胡椒乾粉及油料醬包,以熱壓來密封包裝膜封口,封膜效果好不會溢出,放入熱水中 2 分鐘內皆可完全溶解,攪拌後調味料分布平均,是很適合當作泡麵調味包的包裝材料,故將魚鱗廢棄物再利用,製作的薄膜不但能食用,亦能取代塑膠,預期可達到「低污染、省資源、無廢棄」等減量減廢之環保理念。

Utilization of Escherichia coli in Contiminated Water in the Citarum River as a Dual Chamber Baed On Microbial Fuel Cell (MFC) Substrat

Citarum River is the longest and largest river in West Java. The upstream of the Citarum River starts from Mount Wayang, Bandung Regency and ends at the mouth of the Java Sea which is located in Muara Gembong, Bekasi Regency. The Citarum River plays an important role as raw water for PDAM drinking water, supplies electricity in Java-Bali and provides water for rice field irrigation in West Java. Citarum watershed is dominated by the manufacturing industry sector such as chemicals, textiles, leather, paper, pharmaceuticals, metals, food and beverage products, and others. Based on data from the World Bank, every day, the Citarum River is polluted by approximately 20,000 tons of waste and 340,000 tons of waste water with the majority of the waste contributors coming from 2,000 textile industries. By looking at these events, there is no doubt that the sustainability of the ecosystem and the environment in the Citarum River is damaged and polluted. (Zahra Fani Robyanti; 2020). The West Java Regional Environmental Management Agency stated that the content of E. coli bacteria in the Citarum River had increased. The bacteria that cause diarrhea come from industrial and household waste. In addition to E. coli bacteria, other pollutants in the Citarum River that have increased are biological oxygen demand (BOD), chemical oxygen demand (COD) and Suspended Solids. One of the efforts that can be done regarding E. coli bacteria that pollute the Citarum river is to make it as a substrate for Microbial Fuel Cell (MFC). Bacteria present in organic media convert organic matter into electrical energy. The nature of bacteria that can degrade organic media (enrichment media) in MFC produces electron and proton ions. It is these ions that produce an electric potential difference so that energy can be generated. Generally in conventional systems, MFC consists of two chambers consisting of anode and cathode chambers. The two spaces are separated by a membrane where proton exchange occurs. This system has not fully worked with bacteria because only the anode side contains bacteria, while on the cathode side it still works using chemical compounds such as Polyaluminum Chloride (PAC). However, recently MFC has been developed using bacteria at the cathode, or better known as biocathode. Bacteria in the cathode space have the same function as electron mediators that were previously carried out by chemical compounds. In many studies on MFCs, acetate is commonly used as a substrate for bacteria to generate electricity. These chemical compounds are easier for bacteria to process than wastewater. Acetate is a simple chemical compound that serves as a carbon source for bacteria. Another advantage of acetate is that this compound does not cause other reactions to bacteria such as fermentation and methanogenesis at room temperature. Based on this thought, the author will design a study entitled Utilization of Escherichia coli Bacteria in Contaminated Water in the Citarum River as a Dual Chamber Based Microbial Fuel Cell (MFC) Substrate.

Revolutionizing Potato Agriculture: Harnessing Machine Learning Techniques for Disease Detection and Management

Aim: The aim of this study is to make a disease-predicting model trained on data from weather stations and API using machine learning that gives the farmer the ability to predict crop diseases before they set in, allowing them to take timely preventative measures and reduce wastage. Materials and Methods: In this study the Internet of Things (IoT) sensors throughout agricultural fields of potato crops in Jafferabad, Depalpur Punjab. The sensors collect real-time data on environmental conditions, such as precipitation, air temperature, relative humidity, wind speed, and direction, Dew Point, VPD, and the Delta T values, to identify subtle disease indicators and patterns within the environmental data. Our novel machine-learning program makes use of the data collected by the weather station and analyses them. Results: Using the data, one predictive statistical method using Python 3.8.0 was created which uses the data from the weather station which can predict diseases before they set in.

二氧化碳高選擇性轉化生成合成氣之碳中和工程

近年來碳排放的淨零是全人類所想達成的共同目標,因此本研究透過油浴將鐵、鈷或鎳離子與配位基配位,並形成以2-甲基咪唑為配體的前驅物沸石咪唑骨架,以及以雙氰胺和葡萄糖反應為配體的前驅物類石墨相氮化碳,再鍛燒形成可導電的催化劑。研究者分別以上述兩系列的金屬單分散催化劑,使用氣液分隔的氣體擴散電極進行電解人工光合作用,將CO2高選擇性地還原為CO,並探討各催化劑產CO效率。本研究發現鎳金屬類石墨相氮化碳對CO2還原反應的選擇性極高,能使產CO效率達到99%。而CO作為合成氣已有成熟的工業製程,能生成許多具經濟價值的產物,工業需求量非常大。未來若實際執行,則有助碳中和目標的達成。

研究以微生物分解廢食用油降低其對環境汙染

本研究從餐廳截油槽中採菌,並以含Tween80及Ca2+培養基篩選出具lipase的五種菌。進一步在大豆沙拉油與豬油中培養,發現一號菌及二號菌有較佳的分解能力,二號菌最佳,且二號菌在分解沙拉油(6.0%)的能力大於豬油(4.9%)。將此兩種菌進行深入探討,發現二號菌在處理截油槽中的廢油效能上優於一號菌,且當一、二號菌混合時更佳;於不同油脂Nutrient Broth 培養基的生長情況,二號菌表現比一號菌好。經定序後比對序列後,推斷一號菌接近Serratia marcescens,二號菌較接近Serratia grimesii。總結一、二號菌是具備油脂分解潛力的菌種。 未來規劃,將菌加入自行設計的油脂分解截油槽,比較計算其分解能力,運用於含油脂的廢水處理,減少環境汙染。