全國中小學科展

2014年

本土性微藻結合廢水之生質柴油產製

本研究以淡水常見之微藻進行培養,首先篩選出頂棘藻Chodatella sp.為研究對象,第二階段設計不同曝氣量及不同LED燈之佈光面積進行藻類培養比較,再以部分因素法探討微藻培養之最佳化條件;第三階段設計LED光生物反應器培養微藻,探討其可行性及微藻降解畜牧廢水營養鹽之能力。實驗結果顯示,曝氣的培養方式以及增加佈光面積有助於微藻生長,而部分因素法實驗設計結果,影響生質潛勢最重要因子為置換天數。另外,LED光生物反應器培養的微藻其生質物產率高達230.73 mg / L.d,水中的營養鹽去除率皆高達約90 %以上,藻粉油脂含量為28.77 %,脂肪酸組成顯示碳數具有C16-C18之物質,與生質柴油的組成物質相符,非常具有轉化成生質柴油的潛勢。

蒙地卡羅法模擬光跡與病變診斷驗證

此報告是利用蒙地卡羅法模擬光子在組織內的行徑軌跡,並且依照生物組織成分的光學特性,了解頻譜變化。以皮膚組織為例,可分為表皮與基質,依照其成分巨觀量測到的光學參數(如:折射率、吸收、散射及非均向係數),調整光子微觀的位置、方向和能量,藉此累加統計光子的反射、吸收及穿透狀況,解釋組織光學所觀察到光子走越深穿越遠的現象,與對應生理的巨觀的反射光譜變化。模擬數據中可看出波長越長對於病變組織反射率的變化越為敏感,與文獻中病變資料比較,可對應其提供的結果;並且我們延伸探討在紅外光的結果,此範圍的光為生物窗,其穿透深度較深,可以增加應用範圍。

草藥對癌症轉移之影響

癌症轉移是大部分癌症治療失敗的主因。癌症轉移涉及癌細胞不正常活化周圍蛋白水解酶並將胞外基底膜基質(ECM)降解,讓細胞得以轉移至其他器官。本研究聚焦於研發可以阻止癌細胞侵襲其他組織或轉移的藥物,並期望能以藥物控制蛋白水解酶的活性,進而達到降低癌症轉移的機會。我們發現兩種草藥萃取物(MSL-G和MSL-H)可有效地抑制肝、肺和攝護腺癌細胞的移動侵襲力。實驗結果顯示,此二草藥萃取物可有效抑制基質金屬蛋白酶(matrix metalloproteinase 2 and 9 (MMP2/9))的活性,並促進金屬蛋白酶抑制蛋白(TIMP1)的表現。因此這兩種草藥萃取物可能促使TIMP1的表現進而抑制MMP2/9的活性,或同時具抑制MMP2/9的蛋白酶活化機制,並指出此兩種萃取物具有開發為抗癌藥物之潛力,計畫將利用動物實驗進一步驗證此藥物對癌症轉移的效用。

簡易方法測量勞侖茲力

在一個壓克力製的長方形盒中放入兩層濃度不同的食鹽水,將一隻尖端磨平的針頭水平置放在盒中,針頭出水口恰沒入上層濃度較小的食鹽水中,針頭以橡皮管和滴定管相連,滴定管灌滿染料水溶液,打開活栓瞬間,染料在食鹽水中形成漩渦偶極子,由漩渦偶極子移動距離隨時間的變化,可求得染料作用於食鹽水的慣性力。等位線與電場實驗的電場形成盤放入兩層濃度不同的食鹽水,用兩片平行金屬板做電極,在食鹽水面上放一磁鐵,磁鐵下方滴入數滴染料,食鹽水中的離子受電力、勞侖茲力及黏滯力作用亦形成漩渦偶極子,由漩渦偶極子移動的距離隨時間的變化可求得勞侖茲力。

可重複使用之新型固體酸在藥物合成與生質能的催化應用

生質能源是最佳的能源之一,而固體酸觸媒最能符合綠色化學,因此本研究要開發一種可以水解纖維素得到生質能的固體酸觸媒。將芳香族X與3-氯丙基三甲氧基矽烷反應,產物嫁接至中孔洞SBA-15-p上,再進行磺酸化,得到固體酸TPS-SBA-15-p,將其應用在催化酯化反應及阿斯匹靈的合成,催化效果優於已發表的固體酸觸媒。將TPS-SBA-15-p應用在催化水解纖維素,2 g纖維素與0.5 g觸媒在不鏽鋼高壓反應鍋中加熱至150°C反應7天,轉化率達75.7%,主產物為乙醯丙酸及癸烷,兩者碳數佔全部產物碳數的64%,乙醯丙酸的用途很廣,而癸烷是汽柴油的成分。另外,TPS-SBA-15-p具有兩種催化活性位置,此兩種催化活性官能基具有協同催化作用,因此很容易催化水解二噁烷(dioxane)及苯甲醚,也就具有催化水解纖維素的能力。

以魚類耳石探討A.MAMORATA的生長和迴游特性

鱸鰻(Anguilla marmorata)曾經是台灣的保育類動物,雖然於2009年解除其保育類身分,但對於其生活史目前所知仍然非常有限,相關論文也很少。本研究欲藉由鱸鰻耳石上出現的年輪、日周輪以及微量元素之分析,來重建鰻魚的生長及洄游環境史。我們利用台灣大學漁業科學研究所採自菲律賓的野生鱸鰻和海南島的養殖鱸鰻進行分析,並且和已經發表的日本鰻(A. japonica) 文獻比對,以期歸納出鱸鰻的生活史以及棲地利用的特性。本研究的耳石測量在台灣大學漁業科學研究所進行,魚類耳石鍶鈣比則利用中央研究院地球科學研究所的電子微探儀(EPMA) 進行分析。結果發現鱸鰻自海中孵化後約莫經過130天會回到陸地的淡水環境成長,這與日本鰻回到陸地鹹淡水環境成長的特性有很大差別。這項發現對今後鱸鰻保育工作的環境管理措施,具有參考價值。

以黃色小鴨風潮探討高中生對療癒系物品特質與偏好之研究

本研究主要目的是以黃色小鴨的參觀風潮與商品特質,探討高中生對療癒系物品的偏好為何。黃色小鴨在全球形成一股風潮,許多人認為參觀黃色小鴨有紓壓的功能,也有人認為買小鴨玩偶陪伴自己,有療癒心情的效果,因此,本研究的主軸分別從「黃色小鴨的參觀活動」與「療癒系商品(HSP)特質」兩個大面向進行探討分析,釐清高中生對黃色小鴨療癒系物品(RDRP)的偏好為何。本研究對象為臺灣南部地區的高中生共254位,以「療癒商品與相關活動感受問卷」進行施測,分析結果顯示:(1) 對於療癒商品,女生喜好與購買意願均大於男生,其中又以「仿生造形型」類型最受歡迎;(2) HSP以「規律動作型」、「擬生命行為型」,而RDRP以「仿生造形型」、「手觸摸型」較能藉由放鬆及平衡情緒,達到療癒效果;(3) HSP與RDRP均以「聲控及感音型」較能藉由轉移情緖及產生驚喜,達到療癒效果。

絲絲入扣-可發光高分子奈米纖維之探討

過量的紫外線易引發皮膚病變。因此,我希望找出一種能將紫外光轉為有益皮膚之可見光的方法。我以可發光高分子奈米纖維為素材,嘗試了兩種不同的方法。第一種為利用可發光之PFO、PFBT高分子來製造纖維。發現藉由添加特殊基團(BT)修飾,可改變纖維放光波長。另一種為使用聚乳酸混合聚集誘導發光特性(AIE)小分子。在比較各配方後,以HPS小分子與PLA 120 mg/mL溶於二氯甲烷:二甲基甲醯胺(7:3)製出的纖維最佳。而經由光譜圖的觀察比較可證明:電紡的拉伸特性及奈米尺寸的高比表面積,更能增加纖維的發光效率。期望未來能將其運用於光療面膜、抗紫外線衣物等方面。

Reuse Waste and Save the World by Production Fiber Reinforced 'CB' made from Empty Fruit Bunch (EFB)

At present Malaysia is the largest exporter of palm oil in the international market. In the process of extraction of palm oil from oil palm fruit, biomass materials such as palm empty fruit bunch (EFB) and palm pressed fibre (PPF) are generated as waste products. Natural fibres reinforced cement-based materials have gain increasing application in residential housing components. One of the natural fibres considered is oil palm empty fruit bunches (EFB) fibres which offer advantages such as availability, renewability, low cost and the established technology to extract the fibres. This study investigates the properties of cement board incorporated with large amount of oil palm EFB fibres Among the tests conducted was compressive strength, density, water absorption and thickness swelling tests. It was found that high EFB fibres content lead to lower strength and higher absorption . The results also indicate that high EFB fibres contents reduced the self-weight of the blocks and the resulting blocks can be classified as lightweight cement blocks suitable to be used as lightweight walling materials. Our research is to study the production of cement board using Empty fruit bunch(EFB)These board were made from empty fruit bunch, cement and water. Two chemical are added is aluminium sulphate and sodium silicate. Cement : EFB mixture by weight was 2.5:1, 2.75:1 and 3.0:1 used to produces a cement board.

Antimicrobial and Heavy Metal Sequestration Capacities of Graphene Polymer Nanofilms

Membrane bioreactors (MBR) are important components in the production of effluent in wastewater treatment systems. However, MBR are susceptible to biofouling, a process by which bacteria colonize the surface of the membrane in contact with water. Graphene could be a solution to biofilm formation. In this study, the graphene polymer nanocomposite’s antimicrobial and heavy metal removal properties and the mechanisms behind the properties were investigated. Five different films of nanocomposites with a form of graphene and a polymer were synthesized: Graphene, Graphene Oxide, PVK-GO, PVK-G, PVK. A Büchner funnel and a vacuum pump were used to coat membrane filters with solutions of each nanomaterial. Using the Büchner funnel, E. coli and B. subtilis bacteria were filtered through the filter and both the filtrate and the filter were examined for bacterial content. Similarly, a Pb2+ solution was filtered through the coated filters and percentage removal of the ion was calculated using Atomic Absorbtion Spectrometry. Further analysis from SEM data, ATR-IR, and an Oxidative Stress test revealed that the PVK-GO nanocomposite inactivates bacteria by causing oxidative stress and the carboxyl group binds to lead ions. PVK-GO was most effective at removing the highest percentage of heavy metal and inactivated the most bacteria and displayed the most antimicrobial properties. PVK-GO coatings provide an efficient and economical alternative to the current wastewater industry standard and can save millions of dollars and reduce environmental waste. Also, the coatings have applications in indwelling medical devices and can reduce the risks associated with biofilm formational and bacterial infections.